LOCUS       sequence-424094-       10945 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expression of human DLX2 and GFP under the human GFAP ABC1D 
            promoter.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10945)
  AUTHORS   Zhang Y, Li B, Cananzi S, Han C, Wang LL, Zou Y, Fu YX, Hon GC, 
            Zhang CL
  TITLE     A single factor elicits multilineage reprogramming of astrocytes in 
            the adult mouse striatum.
  JOURNAL   Proc Natl Acad Sci U S A. 2022 Mar 15;119(11):e2107339119. doi: 
            10.1073/pnas.2107339119. Epub 2022 Mar 7.
  PUBMED    35254903
REFERENCE   2  (bases 1 to 10945)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2022-03-15"; volume: "119(11):e2107339119. 
            doi"; pages: " 10.1073/pnas.2107339119. Epub 2022 Mar 7"
COMMENT     Sequence Label: LV-hGFAP-DLX2-ires-GFP
FEATURES             Location/Qualifiers
     source          1..10945
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             262..442
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(468..485)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    474..698
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      744..1172
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(831..850)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1041..1062
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(1181..1201)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        1186..1515
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      1366..1501
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     1428..1447
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        1563..1610
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             1629..2003
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    2133..2254
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(2170..2189)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     2224..2243
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(2303..2319)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2303..2319)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2316..2338)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    2327..2343
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(2351..2357,2358..2375,2376..2381))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2396..2417
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2534..2551)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2705..3293)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2785..2804)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3464..4255,4256..4324))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(4325..4429)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(4504..4523)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        4695..5074
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        5076..5274
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     5229..5249
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             5292..5472
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    5519..5644
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    6137..6370
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6555..6599
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6873..6990
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     CDS             8648..8668
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=7xHis
                     /translation="HHHHHHH"
     misc_feature    8761..9328
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     misc_feature    8779..9330
                     /label=IRES
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8923..8940)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9150..9169
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(9333..9335,9336..9338,9339..10052)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(9333..9335,9336..9338,9339..10049)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(9378..9399)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(9639..9658)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     9986..10007
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    10100..10688
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(10153..10173)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(10571..10582)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 acacaaggct acttccctga ttggcagaac tacacaccag ggccagggat cagatatcca
       61 ctgacctttg gatggtgcta caagctagta ccagttgagc aagagaaggt agaagaagcc
      121 aatgaaggag agaacacccg cttgttacac cctgtgagcc tgcatgggat ggatgacccg
      181 gagagagaag tattagagtg gaggtttgac agccgcctag catttcatca catggcccga
      241 gagctgcatc cggactgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
      301 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
      361 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
      421 tcagtgtgga aaatctctag cagggcccgt ttaaacccgc tgatcagcct cgactgtgcc
      481 ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg
      541 tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag
      601 gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga
      661 caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaaccag
      721 ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
      781 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
      841 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
      901 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgatta
      961 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
     1021 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
     1081 ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
     1141 tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg tcagttaggg
     1201 tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag
     1261 tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     1321 catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact
     1381 ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag
     1441 gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc
     1501 ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc tgatcagcac
     1561 gtgttgacaa ttaatcatcg gcatagtata tcggcatagt ataatacgac aaggtgagga
     1621 actaaaccat ggccaagttg accagtgccg ttccggtgct caccgcgcgc gacgtcgccg
     1681 gagcggtcga gttctggacc gaccggctcg ggttctcccg ggacttcgtg gaggacgact
     1741 tcgccggtgt ggtccgggac gacgtgaccc tgttcatcag cgcggtccag gaccaggtgg
     1801 tgccggacaa caccctggcc tgggtgtggg tgcgcggcct ggacgagctg tacgccgagt
     1861 ggtcggaggt cgtgtccacg aacttccggg acgcctccgg gccggccatg accgagatcg
     1921 gcgagcagcc gtgggggcgg gagttcgccc tgcgcgaccc ggccggcaac tgcgtgcact
     1981 tcgtggccga ggagcaggac tgacacgtgc tacgagattt cgattccacc gccgccttct
     2041 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
     2101 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
     2161 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
     2221 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
     2281 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
     2341 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
     2401 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
     2461 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
     2521 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
     2581 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
     2641 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
     2701 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
     2761 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
     2821 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
     2881 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
     2941 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
     3001 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
     3061 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
     3121 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
     3181 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
     3241 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
     3301 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
     3361 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
     3421 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
     3481 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
     3541 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
     3601 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
     3661 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
     3721 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
     3781 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
     3841 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
     3901 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
     3961 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
     4021 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
     4081 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
     4141 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
     4201 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
     4261 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
     4321 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
     4381 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
     4441 gaaaagtgcc acctgacgtc gacggatcgg gagatctccc gatcccctat ggtgcactct
     4501 cagtacaatc tgctctgatg ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt
     4561 ggaggtcgct gagtagtgcg cgagcaaaat ttaagctaca acaaggcaag gcttgaccga
     4621 caattgcatg aagaatctgc ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc
     4681 cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     4741 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     4801 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     4861 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     4921 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     4981 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     5041 gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc agtacatcaa
     5101 tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca ttgacgtcaa
     5161 tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta acaactccgc
     5221 cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa gcagcgcgtt
     5281 ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac
     5341 tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg
     5401 cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga
     5461 aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac cagaggagct
     5521 ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg ggcggcgact
     5581 ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg ggtgcgagag
     5641 cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt taaggccagg
     5701 gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc tagaacgatt
     5761 cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac tgggacagct
     5821 acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata cagtagcaac
     5881 cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt tagacaagat
     5941 agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg atcttcagac
     6001 ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat aaagtagtaa
     6061 aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg cagagagaaa
     6121 aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca ggaagcacta
     6181 tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct ggtatagtgc
     6241 agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg caactcacag
     6301 tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac ctaaaggatc
     6361 aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact gctgtgcctt
     6421 ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg acctggatgg
     6481 agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt gaagaatcgc
     6541 aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg gcaagtttgt
     6601 ggaattggtt taacataaca aattggctgt ggtatataaa attattcata atgatagtag
     6661 gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat agagttaggc
     6721 agggatattc accattatcg tttcagaccc acctcccaac cccgagggga cccgacaggc
     6781 ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt cgattagtga
     6841 acggatctac aaatggcagt attcatccac aattttaaaa gaaaaggggg gattgggggg
     6901 tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac taaagaatta
     6961 caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acagggacag cagaaattca
     7021 ctttgaattc aacatatcct ggtgtggagt aggggacgct gctctgacag aggctcgggg
     7081 gcctgagctg gctctgtgag ctggggagga ggcagacagc caggccttgt ctgcaagcag
     7141 acctggcagc attgggctgg ccgcccccca gggcctcctc ttcatgccca gtgaatgact
     7201 caccttggca cagacacaat gttcggggtg ggcacagtgc ctgcttcccg ccgcacccca
     7261 gcccccctca aatgccttcc gagaagccca ttgagcaggg ggcttgcatt gcaccccagc
     7321 ctgacagcct ggcatcttgg gataaaagca gcacagcccc ctaggggctg cccttgctgt
     7381 gtggcgccac cggcggtgga gaacaaggct ctattcagcc tgtgcccagg aaaggggatc
     7441 aggggatgcc caggcatgga cagtgggtgg caggggggga gaggagggct gtctgcttcc
     7501 cagaagtcca aggacacaaa tgggtgaggg gagagctctc cccatagctg ggctgcggcc
     7561 caaccccacc ccctcaggct atgccagggg gtgttgccag gggcacccgg gcatcgccag
     7621 tctagcccac tccttcataa agccctcgca tcccaggagc gagcagagcc agagcaggtt
     7681 ggagaggaga cgcatcacct ccgctgctcg caccggtgcc accatgactg gagtctttga
     7741 cagtctagtg gctgatatgc actcgaccca gatcgccgcc tccagcacgt accaccagca
     7801 ccagcagccc ccgagcggcg gcggcgccgg cccgggtggc aacagcagca gcagcagcag
     7861 cctccacaag ccccaggagt cgcccaccct tccggtgtcc accgccaccg acagcagcta
     7921 ctacaccaac cagcagcacc cggcgggcgg cggcggcggc gggggctcgc cctacgcgca
     7981 catgggttcc taccagtacc aagccagcgg cctcaacaac gtcccttact ccgccaagag
     8041 cagctatgac ctgggctaca ccgccgccta cacctcctac gctccctatg gaaccagttc
     8101 gtccccagcc aacaacgagc ctgagaagga ggaccttgag cctgaaattc ggatagtgaa
     8161 cgggaagcca aagaaagtcc ggaaaccccg caccatctac tccagtttcc agctggcggc
     8221 tcttcagcgg cgtttccaaa agactcaata cttggccttg ccggagcgag ccgagctggc
     8281 ggcctctctg ggcctcaccc agactcaggt caaaatctgg ttccagaacc gccggtccaa
     8341 gttcaagaag atgtggaaaa gtggtgagat cccctcggag cagcaccctg gggccagcgc
     8401 ttctccacct tgtgcttcgc cgccagtctc agcgccggcc tcctgggact ttggtgtgcc
     8461 gcagcggatg gcgggcggcg gtggtccggg cagtggcggc agcggcgccg gcagctcggg
     8521 ctccagcccg agcagcgcgg cctcggcttt tctgggcaac tacccctggt accaccagac
     8581 ctcgggatcc gcctcacacc tgcaggccac ggcgccgctg ctgcacccca ctcagacccc
     8641 gcagccgcat caccaccacc accatcacgg cggcgggggc gccccggtga gcgcggggac
     8701 gattttctaa tgtacaagtc cggactcaga tcctactagt aggatctcga gatgcagaat
     8761 tccgcccccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
     8821 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
     8881 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
     8941 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
     9001 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
     9061 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
     9121 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
     9181 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
     9241 gctttacatg tgtttagtcg aggttaaaaa acgtctaggc cccccgaacc acggggacgt
     9301 ggttttcctt tgaaaaacac gatgataata ccatggtgag caagggcgag gagctgttca
     9361 ccggggtggt gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg
     9421 tgtccggcga gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca
     9481 ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc
     9541 agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc
     9601 ccgaaggcta cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc
     9661 gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg
     9721 acttcaagga ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca
     9781 acgtctatat catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc
     9841 acaacatcga ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg
     9901 gcgacggccc cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca
     9961 aagaccccaa cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga
    10021 tcactctcgg catggacgag ctgtacaagt aaagcggccg cgactctaga gtcgagtttg
    10081 agggatcaag cttatcgata atcaacctct ggattacaaa atttgtgaaa gattgactgg
    10141 tattcttaac tatgttgctc cttttacgct atgtggatac gctgctttaa tgcctttgta
    10201 tcatgctatt gcttcccgta tggctttcat tttctcctcc ttgtataaat cctggttgct
    10261 gtctctttat gaggagttgt ggcccgttgt caggcaacgt ggcgtggtgt gcactgtgtt
    10321 tgctgacgca acccccactg gttggggcat tgccaccacc tgtcagctcc tttccgggac
    10381 tttcgctttc cccctcccta ttgccacggc ggaactcatc gccgcctgcc ttgcccgctg
    10441 ctggacaggg gctcggctgt tgggcactga caattccgtg gtgttgtcgg ggaagctgac
    10501 gtcctttcca tggctgctcg cctgtgttgc cacctggatt ctgcgcggga cgtccttctg
    10561 ctacgtccct tcggccctca atccagcgga ccttccttcc cgcggcctgc tgccggctct
    10621 gcggcctctt ccgcgtcttc gccttcgccc tcagacgagt cggatctccc tttgggccgc
    10681 ctccccgcat cgataccgtc gagacctaga aaaacatgga gcaatcacaa gtagcaatac
    10741 agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt
    10801 tccagtcaca cctcaggtac ctttaagacc aatgacttac aaggcagctg tagatcttag
    10861 ccacttttta aaagaaaagg ggggactgga agggctaatt cactcccaac gaagacaaga
    10921 tatccttgat ctgtggatct accac
//
