LOCUS       sequence-424112-       10371 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synchronize trafficking of ManII from the ER (RUSH system).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10371)
  AUTHORS   Boncompain G, Divoux S, Gareil N, de Forges H, Lescure A, Latreche 
            L, Mercanti V, Jollivet F, Raposo G, Perez F
  TITLE     Synchronization of secretory protein traffic in populations of 
            cells.
  JOURNAL   Nat Methods. 2012 Mar 11;9(5):493-8. doi: 10.1038/nmeth.1928.
  PUBMED    22406856
REFERENCE   2  (bases 1 to 10371)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/nmeth"; 
            journalName: "Nat Methods"; date: "2012-03-11- 11"; volume: "9"; 
            issue: "5"; pages: "493-8"
COMMENT     Sequence Label: pCDH_Str-KDEL_ManII-SBP-EGFP
FEATURES             Location/Qualifiers
     source          1..10371
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        34..237
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     188..208
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     234..258
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          1027..1256
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(1329..1903,1904..1906,1907..1915)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(1496..1513)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     1723..1742
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             2304..2417
                     /codon_start=1
                     /product="streptavidin-binding peptide"
                     /label=SBP
                     /note="selected from a peptide library; binds streptavidin 
                     with nanomolar affinity (Keefe et al., 2001)"
                     /translation="MDEKTTGWRGGHVVEGLAGELEQLRARLEHHPQGQREP"
     CDS             join(2427..2429,2430..2432,2433..3143)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTASGITLGMDELYK"
     primer_bind     complement(2472..2493)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2733..2752)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3080..3101
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     promoter        3346..3557
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             3570..3838
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     CDS             3865..4464
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(3865..3884)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     4361..4381
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    4471..5059
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4524..4544)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4942..4953)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5133..5366
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5438..5559
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5475..5494)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     5529..5548
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      5599..5734
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5767..5783)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(5767..5783)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5780..5802)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5791..5807
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5815..5821,5822..5839,5840..5845))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5860..5881
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5998..6015)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6169..6757)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(6928..7719,7720..7788))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7551..7570
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7789..7893)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     7961..7979
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8017..8039)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     8139..8158
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     8352..8374
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     8366..8383
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     8367..8383
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        8398..8624
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             8625..8805
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8852..8977
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    9470..9703
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9888..9932
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    10199..10315
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca gtacatcaat
       61 gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat tgacgtcaat
      121 gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa caactccgcc
      181 ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tttatataag cagagctcgt
      241 ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct ccatagaaga
      301 ttctagagct agcgcttaag gcctgttaac cggtatggat gtatgcgtcc gtcttgccct
      361 gtggctcctc tggggactcc tcctgcacca gggccagagc ctcagccata gtcacagtga
      421 gaaggcgaca ggaaccagct cgggcgccgg tttaaacgac cctagcaaag actcaaaagc
      481 tcaggtgtcc gctgccgagg ctggcattac tggaacatgg tacaatcagc tcgggagcac
      541 ctttattgtg actgctggag ccgatggagc cctcaccgga acatacgaat ctgctgtggg
      601 aaacgccgaa tcacggtacg tcctcactgg ccgatacgat agtgcccctg ccaccgacgg
      661 atctgggact gccctgggat ggactgtcgc ttggaaaaac aactaccgga atgctcattc
      721 tgccacaaca tggagtggac agtacgtggg aggcgctgag gctagaatca atacacagtg
      781 gctgctcaca tctggcacaa ccgaggcaaa tgcttggaaa tccaccctgg tgggacatga
      841 cacattcacc aaagtgaaac cctccgccgc ttcaatcgat gccgccaaaa aagccggagt
      901 caacaacggc aatcctctgg atgccgtcca gcagctcgga ccgaaagatg aactgtgagc
      961 ggccgcatag ataactgatc cagtgtgctg gaattaattc gctgtctgcg agggccagct
     1021 gttggggtga gtactccctc tcaaaagcgg gcatgacttc tgcgctaaga ttgtcagttt
     1081 ccaaaaacga ggaggatttg atattcacct ggcccgcggt gatgcctttg agggtggccg
     1141 cgtccatctg gtcagaaaag acaatctttt tgttgtcaag cttgaggtgt ggcaggcttg
     1201 agatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaactgc aggtcgagca tgcatctagg gcggccaatt ccgcccctct
     1321 cccccccccc cctctccctc ccccccccct aacgttactg gccgaagccg cttggaataa
     1381 ggccggtgtg cgtttgtcta tatgttattt tccaccatat tgccgtcttt tggcaatgtg
     1441 agggcccgga aacctggccc tgtcttcttg acgagcattc ctaggggtct ttcccctctc
     1501 gccaaaggaa tgcaaggtct gttgaatgtc gtgaaggaag cagttcctct ggaagcttct
     1561 tgaagacaaa caacgtctgt agcgaccctt tgcaggcagc ggaacccccc acctggcgac
     1621 aggtgcctct gcggccaaaa gccacgtgta taagatacac ctgcaaaggc ggcacaaccc
     1681 cagtgccacg ttgtgagttg gatagttgtg gaaagagtca aatggctctc ctcaagcgta
     1741 ttcaacaagg ggctgaagga tgcccagaag gtaccccatt gtatgggatc tgatctgggg
     1801 cctcggtgca catgctttac atgtgtttag tcgaggttaa aaaaacgtct aggccccccg
     1861 aaccacgggg acgtggtttt cctttgaaaa acacgatgat aagcttgcca caacccggga
     1921 ggcgcgccat gaagttaagt cgccagttca ccgtgtttgg cagcgcgatc ttctgcgtcg
     1981 taatcttctc actctacctg atgctggaca ggggtcactt ggactaccct cggggcccgc
     2041 gccaggaggg ctcctttccg cagggccagc tttcaatatt gcaagaaaag attgaccatt
     2101 tggagcgttt gctcgctgag aacaacgaga ttatctcaaa tatcagagac tcagtcatca
     2161 acctgagcga gtctgtggag gacggcccgc gggggtcacc aggcaacgcc agccaaggct
     2221 ccatccacct ccactcgcca cagttggccc tgcaggctga ccccagagac tgtttggatc
     2281 ccaccggtcg ccaccggaat tccatggacg agaagaccac tggttggcga ggtggacacg
     2341 ttgttgaagg actggctggg gaacttgaac aacttcgtgc acgactggag catcacccac
     2401 aaggtcaacg tgaaccacct gcaggtatgg tgagcaaagg cgaggagctg ttcaccgggg
     2461 tggtgcccat cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg
     2521 gcgagggcga gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg
     2581 gcaagctgcc cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct
     2641 tcagccgcta ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag
     2701 gctacgtcca ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg
     2761 aggtgaagtt cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca
     2821 aggaggacgg caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct
     2881 atatcatggc cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca
     2941 tcgaggacgg cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg
     3001 gccccgtgct gctgcccgac aaccactacc tgagcaccca gtccgccctg agcaaagacc
     3061 ccaacgagaa gcgcgatcac atggtcctgc tggagttcgt gaccgcctcc gggatcactc
     3121 tcggcatgga cgagctgtac aagggccggc cttaaggcct cgagggccaa ttaattaact
     3181 ctagataact gatcataatc agccatacca catttgtaga ggttttactt gctttaaaaa
     3241 acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattcga atttaaatgg
     3301 atccgcggcc gcgaaggatc tgcgatcgct ccggtgcccg tcagtgggca gagcgcacat
     3361 cgcccacagt ccccgagaag ttggggggag gggtcggcaa ttgaacgggt gcctagagaa
     3421 ggtggcgcgg ggtaaactgg gaaagtgatg tcgtgtactg gctccgcctt tttcccgagg
     3481 gtgggggaga accgtatata agtgcagtag tcgccgtgaa cgttcttttt cgcaacgggt
     3541 ttgccgccag aacacagctg aagcttcgag gggctcgcat ctctccttca cgcgcccgcc
     3601 gccctacctg aggccgccat ccacgccggt tgagtcgcgt tctgccgcct cccgcctgtg
     3661 gtgcctcctg aactgcgtcc gccgtctagg taagtttaaa gctcaggtcg agaccgggcc
     3721 tttgtccggc gctcccttgg agcctaccta gactcagccg gctctccacg ctttgcctga
     3781 ccctgcttgc tcaactctac gtctttgttt cgttttctgt tctgcgccgt tacagatcca
     3841 agctgtgacc ggcgcctacg ctagatgacc gagtacaagc ccacggtgcg cctcgccacc
     3901 cgcgacgacg tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc
     3961 acgcgccaca ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc
     4021 ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg
     4081 gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc
     4141 ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc
     4201 ctggcgccgc accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc
     4261 gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag
     4321 cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag
     4381 cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc
     4441 atgacccgca agcccggtgc ctgagtcgac aatcaacctc tggattacaa aatttgtgaa
     4501 agattgactg gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta
     4561 atgcctttgt atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa
     4621 tcctggttgc tgtctcttta tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg
     4681 tgcactgtgt ttgctgacgc aacccccact ggttggggca ttgccaccac ctgtcagctc
     4741 ctttccggga ctttcgcttt ccccctccct attgccacgg cggaactcat cgccgcctgc
     4801 cttgcccgct gctggacagg ggctcggctg ttgggcactg acaattccgt ggtgttgtcg
     4861 gggaaatcat cgtcctttcc ttggctgctc gcctgtgttg ccacctggat tctgcgcggg
     4921 acgtccttct gctacgtccc ttcggccctc aatccagcgg accttccttc ccgcggcctg
     4981 ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc ctcagacgag tcggatctcc
     5041 ctttgggccg cctccccgcc tggtaccttt aagaccaatg acttacaagg cagctgtaga
     5101 tcttagccac tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag
     5161 ataagatctg ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga
     5221 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     5281 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     5341 ttagtcagtg tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt
     5401 ataacttgca aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa
     5461 tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca
     5521 ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc
     5581 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     5641 catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta
     5701 ttccagaagt agtgaggagg cttttttgga ggcctagact tttgcagaga ccaaattcgt
     5761 aatcatgtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     5821 acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct aactcacatt
     5881 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     5941 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     6001 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     6061 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     6121 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     6181 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     6241 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     6301 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     6361 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     6421 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     6481 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     6541 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     6601 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     6661 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     6721 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     6781 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     6841 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     6901 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     6961 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     7021 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     7081 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     7141 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     7201 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     7261 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     7321 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     7381 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     7441 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     7501 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     7561 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     7621 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     7681 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     7741 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     7801 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     7861 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga
     7921 cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc
     7981 ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     8041 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     8101 agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact
     8161 gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa aataccgcat
     8221 caggcgccat tcgccattca ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc
     8281 ttcgctatta cgccagctgg cgaaaggggg atgtgctgca aggcgattaa gttgggtaac
     8341 gccagggttt tcccagtcac gacgttgtaa aacgacggcc agtgccaagc tgacgcgtgt
     8401 agtcttatgc aatactcttg tagtcttgca acatggtaac gatgagttag caacatgcct
     8461 tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga agtaaggtgg tacgatcgtg
     8521 ccttattagg aaggcaacag acgggtctga catggattgg acgaaccact gaattgccgc
     8581 attgcagaga tattgtattt aagtgcctag ctcgatacat aaacgggtct ctctggttag
     8641 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
     8701 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
     8761 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
     8821 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
     8881 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
     8941 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
     9001 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
     9061 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
     9121 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
     9181 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     9241 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     9301 caccgcacag caagcggcca ctgatcttca gacctggagg aggagatatg agggacaatt
     9361 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     9421 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     9481 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     9541 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     9601 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     9661 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     9721 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     9781 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     9841 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     9901 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     9961 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
    10021 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
    10081 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
    10141 agagagacag agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt
    10201 ttaaaagaaa aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag
    10261 caacagacat acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga
    10321 tactagtatt atgcccagta catgacctta tgggactttc ctacttggca g
//
