LOCUS       sequence-424142-        3230 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3230)
  AUTHORS   Storz P, Doppler H, Toker A
  TITLE     Protein kinase Cdelta selectively regulates protein kinase 
            D-dependent activation of NF-kappaB in oxidative stress signaling.
  JOURNAL   Mol Cell Biol. 2004 Apr . 24(7):2614-26.
  PUBMED    15024053
REFERENCE   2  (bases 1 to 3230)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Biol. 2004 Apr . 24(7):2614-26."
COMMENT     Sequence Label: pSUPER.PKCdelta.RNAi
FEATURES             Location/Qualifiers
     source          1..3230
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      708..1296
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     1197..1216
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     1450..1467
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    1584..1605
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(1620..1625,1626..1643,1644..1650)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1658..1674
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     1663..1685
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     1682..1698
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     1682..1698
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        1719..1737
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     1765..1781
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     1766..1782
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     promoter        complement(1855..2069)
                     /label=H1 promoter
                     /note="human H1 RNA promoter"
     primer_bind     complement(1915..1934)
                     /label=H1
                     /note="Human H1 promoter, forward primer"
     primer_bind     complement(2089..2105)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2089..2105)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(2137..2156)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2138..2156)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2163..2180)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2163..2179)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2172..2194)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      2321..2776
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2408..2427)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2618..2639
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2802..2906
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2907..2975,2976..3230,1..537)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(3125..3144)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
       61 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
      121 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
      181 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
      241 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
      301 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
      361 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
      421 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
      481 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
      541 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
      601 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
      661 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
      721 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
      781 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
      841 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
      901 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
      961 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     1021 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     1081 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     1141 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     1201 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     1261 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
     1321 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
     1381 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
     1441 accgagcgca gcgagtcagt gagcgaggaa gcggaagagc gcccaatacg caaaccgcct
     1501 ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc cgactggaaa
     1561 gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc accccaggct
     1621 ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata acaatttcac
     1681 acaggaaaca gctatgacca tgattacgcc aagctcgaaa ttaaccctca ctaaagggaa
     1741 caaaagctgg taccgggccc cccctcgagg tcgacggtat cgataagctt ttccaaaaag
     1801 acaagatcat cggcagattc tcttgaaatc tgccgatgat cttgtcgggg atctgtggtc
     1861 tcatacagaa cttataagat tcccaaatcc aaagacattt cacgtttatg gtgatttccc
     1921 agaacacata gcgacatgca aatattgcag ggcgccactc ccctgtccct cacagccatc
     1981 ttcctgccag ggcgcacgcg cgctgggtgt tcccgcctag tgacactggg cccgcgattc
     2041 cttggagcgg gttgatgacg tcagcgttcg aattcctgca gcccggggga tccactagtt
     2101 ctagagcggc cgccaccgcg gtggagctcc aattcgccct atagtgagtc gtattacaat
     2161 tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat
     2221 cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat
     2281 cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg tagcggcgca
     2341 ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcccta
     2401 gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt
     2461 caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac
     2521 cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg atagacggtt
     2581 tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga
     2641 acaacactca accctatctc ggtctattct tttgatttat aagggatttt gccgatttcg
     2701 gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata
     2761 ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     2821 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     2881 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     2941 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     3001 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     3061 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     3121 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     3181 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
//
