LOCUS       sequence-42420-e         835 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the reporter gene under the control of the 
            MiniPromoter in this plasmid has not been tested.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 835)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 835)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1111
FEATURES             Location/Qualifiers
     source          1..835
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     3..21
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     CDS             250..261
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
ORIGIN
        1 aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt aattccgatc atattcaata
       61 acccttaatg aattcgagct cctaggccgg ccagggagag gtgccgtggt gagactgacc
      121 ctcgggccca cgggtggatg atggcaaagg tcctgtggca ggtgtgggag aagcagggct
      181 gcggccagct gccctgagag gcttcaaatt caggccagat ctgctctggg caagaggaag
      241 ctgtgggttt gggagcttca gagacagggg agcaggcctg tcactcaccc tcttttcctt
      301 aaaggctgtc acccccagca gtgcaccctg cagcctgcct atcccctgtg cagctccagc
      361 tccgtctgtc cccagcaatg caccccacgc ccatgtcccc cactccctat gatgctctcg
      421 cgccttctgg agcagcagtg gtaccagctg gaggctgtgg aaaaattgag aggagacaaa
      481 agtgggaaga gcaggccgtg gagagagtgt ctaaacacag ggactatttg gagattgtct
      541 tatttttggt ttgaaatggg cccacctgct agtattgtac aaacgggctc ttcagagcca
      601 tctcagaagg gacagcatcc gcctgtctac ttcaactccc actgatgacg tccatgtgtc
      661 attagtgcca attagaggag ggcagcaggc tgagtgcttg gcctggggcg caagcttgtg
      721 ggagggaaaa ttggattccc cgctagacaa atgtgattac ccgtgctgcc tggacccacc
      781 ccattcagga ccagggcata aatggccagg tgggaccaga gagctcaccc cagcc
//
