LOCUS       sequence-424237-       17742 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gene expression by Piggybac transposase in mammalian cells.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 17742)
  TITLE     Yoshimatsu Lab plasmids 2023
REFERENCE   2  (bases 1 to 17742)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pPB-CAG-PlatTET-wpA-pPuroTK
FEATURES             Location/Qualifiers
     source          1..17742
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    19..39
                     /gene="mutant version of attB"
                     /label=attB3
                     /bound_moiety="BP Clonase(TM)"
                     /note="core recombination site for the Gateway(R) BP 
                     reaction"
     CDS             66..86
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     regulatory      135..144
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(141..167,168..170,171..2657,2658..2660,2661..4244)
                     /codon_start=1
                     /product="catalytically dead mutant of the Cas9 
                     endonuclease from the Streptococcus pyogenes Type II 
                     CRISPR/Cas system"
                     /label=dCas9
                     /note="RNA-guided DNA-binding protein that lacks 
                     endonuclease activity due to the D10A mutation in the RuvC 
                     catalytic domain and the H840A mutation in the HNH 
                     catalytic domain"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             141..4244
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLAIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDAIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             4248..4274
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             4254..4280
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             4293..4313
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4320..4340
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             4404..4460
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4527..4583
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4650..4706
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4773..4829
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             4896..4952
                     /codon_start=1
                     /product="GCN4 peptide optimized to improve solubility 
                     while preserving binding to an scFv-GFP fusion protein 
                     (Tanenbaum et al., 2014)"
                     /label=GCN4_v4
                     /translation="EELLSKNYHLENEVARLKK"
     CDS             5040..5096
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5844..5870
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             5961..6671
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Pedelacq et al., 2006)"
                     /label=superfolder GFP
                     /note="mammalian codon-optimized"
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     CDS             5961..6671
                     /codon_start=1
                     /product="GFP variant that folds robustly even when fused 
                     to poorly folded proteins (Nager et al., 2011)"
                     /label=superfolder GFP
                     /translation="SKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLKF
                     ICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKRHDFFKSAMPEGYVQERTISFKDDGT
                     YKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKAN
                     FKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLEF
                     VTAAGITHGMDELYK"
     primer_bind     complement(5988..6016)
                     /label=GFP-R
                     /note="GFP, reverse primer. Does NOT anneal to EGFP"
     primer_bind     6611..6631
                     /label=GFP-F
                     /note="GFP, forward primer. Does NOT anneal to EGFP"
     protein_bind    complement(8885..8909)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     misc_feature    8935..9524
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8988..9008)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(9406..9417)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    9810..9931
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9847..9866)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9901..9920
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     promoter        10112..10611
                     /label=PGK promoter
                     /note="mouse phosphoglycerate kinase 1 promoter"
     primer_bind     complement(10147..10170)
                     /label=MSCV-rev
                     /note="Murine stem cell virus, reverse primer"
     primer_bind     10544..10563
                     /label=mPGK-F
                     /note="Mouse PGK promoter, forward primer"
     CDS             10635..11231
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(10635..10654)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     11131..11151
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     CDS             11238..12233
                     /codon_start=1
                     /product="truncated herpes simplex virus thymidine kinase 
                     (Salomon et al., 1995)"
                     /label=Delta-TK
                     /note="confers sensitivity to nucleoside analogs such as 
                     acyclovir or ganciclovir"
                     /translation="MPTLLRVYIDGPHGMGKTTTTQLLVALGSRDDIVYVPEPMTYWRV
                     LGASETIANIYTTQHRLDQGEISAGDAAVVMTSAQITMGMPYAVTDAVLAPHIGGEAGS
                     SHAPPPALTLIFDRHPIAALLCYPAARYLMGSMTPQAVLAFVALIPPTLPGTNIVLGAL
                     PEDRHIDRLAKRQRPGERLDLAMLAAIRRVYGLLANTVRYLQCGGSWREDWGQLSGTAV
                     PPQGAEPQSNAGPRPHIGDTLFTLFRAPELLAPNGDLYNVFAWALDVLAKRLRSMHVFI
                     LDYDQSPAGCRDALLQLTSGMVQTHVTTPGSIPTICDLARTFAREMGEAN"
     repeat_region   12759..12821
                     /label=piggyBac right (3') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     promoter        complement(12845..12863)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(12884..12900)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(12884..12900)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(12897..12919)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    12908..12924
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(12932..12938,12939..12956,12957..12962))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    12977..12998
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(13115..13132)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(13286..13874)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(13366..13385)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(14045..14836,14837..14905))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     14668..14687
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(14906..15010)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(15036..15491)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(15173..15194)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     15385..15404
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     15618..15640
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     15632..15649
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     15633..15649
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     15656..15675
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        15656..15674
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     repeat_region   15693..15727
                     /label=piggyBac left (5') inverted repeat
                     /note="piggyBac transposon-specific inverted terminal 
                     repeat sequence (ITR)"
     enhancer        16005..16388
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        16393..16670
                     /label=chicken beta-actin promoter
     intron          16671..17687
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     17611..17633
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     17695..17714
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
ORIGIN
        1 tcgagtctag agtcggggca actttgtata ataaagttga attcccccgc gatcgcgcca
       61 ccatgcccaa gaagaagcgc aaggtgggac gcgtctgcag gatatcaagc ttgcggtacc
      121 gcgggcccgg gatcgccacc atggacaaga agtacagcat cggcctggcc atcggcacca
      181 actctgtggg ctgggccgtg atcaccgacg agtacaaggt gcccagcaag aaattcaagg
      241 tgctgggcaa caccgaccgg cacagcatca agaagaacct gatcggcgcc ctgctgttcg
      301 acagcggaga aacagccgag gccacccggc tgaagagaac cgccagaaga agatacacca
      361 gacggaagaa ccggatctgc tatctgcaag agatcttcag caacgagatg gccaaggtgg
      421 acgacagctt cttccacaga ctggaagagt ccttcctggt ggaagaggat aagaagcacg
      481 agcggcaccc catcttcggc aacatcgtgg acgaggtggc ctaccacgag aagtacccca
      541 ccatctacca cctgagaaag aaactggtgg acagcaccga caaggccgac ctgcggctga
      601 tctatctggc cctggcccac atgatcaagt tccggggcca cttcctgatc gagggcgacc
      661 tgaaccccga caacagcgac gtggacaagc tgttcatcca gctggtgcag acctacaacc
      721 agctgttcga ggaaaacccc atcaacgcca gcggcgtgga cgccaaggcc atcctgtctg
      781 ccagactgag caagagcaga cggctggaaa atctgatcgc ccagctgccc ggcgagaaga
      841 agaatggcct gttcggcaac ctgattgccc tgagcctggg cctgaccccc aacttcaaga
      901 gcaacttcga cctggccgag gatgccaaac tgcagctgag caaggacacc tacgacgacg
      961 acctggacaa cctgctggcc cagatcggcg accagtacgc cgacctgttt ctggccgcca
     1021 agaacctgtc cgacgccatc ctgctgagcg acatcctgag agtgaacacc gagatcacca
     1081 aggcccccct gagcgcctct atgatcaaga gatacgacga gcaccaccag gacctgaccc
     1141 tgctgaaagc tctcgtgcgg cagcagctgc ctgagaagta caaagagatt ttcttcgacc
     1201 agagcaagaa cggctacgcc ggctacatcg atggcggagc cagccaggaa gagttctaca
     1261 agttcatcaa gcccatcctg gaaaagatgg acggcaccga ggaactgctc gtgaagctga
     1321 acagagagga cctgctgcgg aagcagcgga ccttcgacaa cggcagcatc ccccaccaga
     1381 tccacctggg agagctgcac gccattctgc ggcggcagga agatttttac ccattcctga
     1441 aggacaaccg ggaaaagatc gagaagatcc tgaccttccg catcccctac tacgtgggcc
     1501 ctctggccag gggaaacagc agattcgcct ggatgaccag aaagagcgag gaaaccatca
     1561 ccccctggaa cttcgaggaa gtggtggaca agggcgccag cgcccagagc ttcatcgagc
     1621 ggatgaccaa cttcgataag aacctgccca acgagaaggt gctgcccaag cacagcctgc
     1681 tgtacgagta cttcaccgtg tacaacgagc tgaccaaagt gaaatacgtg accgagggaa
     1741 tgagaaagcc cgccttcctg agcggcgagc agaaaaaagc catcgtggac ctgctgttca
     1801 agaccaaccg gaaagtgacc gtgaagcagc tgaaagagga ctacttcaag aaaatcgagt
     1861 gcttcgactc cgtggaaatc tccggcgtgg aagatcggtt caacgcctcc ctgggcacat
     1921 accacgatct gctgaaaatt atcaaggaca aggacttcct ggacaatgag gaaaacgagg
     1981 acattctgga agatatcgtg ctgaccctga cactgtttga ggacagagag atgatcgagg
     2041 aacggctgaa aacctatgcc cacctgttcg acgacaaagt gatgaagcag ctgaagcggc
     2101 ggagatacac cggctggggc aggctgagcc ggaagctgat caacggcatc cgggacaagc
     2161 agtccggcaa gacaatcctg gatttcctga agtccgacgg cttcgccaac agaaacttca
     2221 tgcagctgat ccacgacgac agcctgacct ttaaagagga catccagaaa gcccaggtgt
     2281 ccggccaggg cgatagcctg cacgagcaca ttgccaatct ggccggcagc cccgccatta
     2341 agaagggcat cctgcagaca gtgaaggtgg tggacgagct cgtgaaagtg atgggccggc
     2401 acaagcccga gaacatcgtg atcgaaatgg ccagagagaa ccagaccacc cagaagggac
     2461 agaagaacag ccgcgagaga atgaagcgga tcgaagaggg catcaaagag ctgggcagcc
     2521 agatcctgaa agaacacccc gtggaaaaca cccagctgca gaacgagaag ctgtacctgt
     2581 actacctgca gaatgggcgg gatatgtacg tggaccagga actggacatc aaccggctgt
     2641 ccgactacga tgtggacgct atcgtgcctc agagctttct gaaggacgac tccatcgata
     2701 acaaagtgct gactcggagc gacaagaacc ggggcaagag cgacaacgtg ccctccgaag
     2761 aggtcgtgaa gaagatgaag aactactggc gccagctgct gaatgccaag ctgattaccc
     2821 agaggaagtt cgacaatctg accaaggccg agagaggcgg cctgagcgaa ctggataagg
     2881 ccggcttcat caagagacag ctggtggaaa cccggcagat cacaaagcac gtggcacaga
     2941 tcctggactc ccggatgaac actaagtacg acgagaacga caaactgatc cgggaagtga
     3001 aagtgatcac cctgaagtcc aagctggtgt ccgatttccg gaaggatttc cagttttaca
     3061 aagtgcgcga gatcaacaac taccaccacg cccacgacgc ctacctgaac gccgtcgtgg
     3121 gaaccgccct gatcaaaaag taccctaagc tggaaagcga gttcgtgtac ggcgactaca
     3181 aggtgtacga cgtgcggaag atgatcgcca agagcgagca ggaaatcggc aaggctaccg
     3241 ccaagtactt cttctacagc aacatcatga actttttcaa gaccgagatt accctggcca
     3301 acggcgagat ccggaagcgg cctctgatcg agacaaacgg cgaaacaggc gagatcgtgt
     3361 gggataaggg ccgggacttt gccaccgtgc ggaaagtgct gtctatgccc caagtgaata
     3421 tcgtgaaaaa gaccgaggtg cagacaggcg gcttcagcaa agagtctatc ctgcccaaga
     3481 ggaacagcga caagctgatc gccagaaaga aggactggga ccctaagaag tacggcggct
     3541 tcgacagccc caccgtggcc tattctgtgc tggtggtggc caaagtggaa aagggcaagt
     3601 ccaagaaact gaagagtgtg aaagagctgc tggggatcac catcatggaa agaagcagct
     3661 tcgagaagaa tcccatcgac tttctggaag ccaagggcta caaagaagtg aaaaaggacc
     3721 tgatcatcaa gctgcctaag tactccctgt tcgagctgga aaacggccgg aagagaatgc
     3781 tggcctctgc cggcgaactg cagaagggaa acgaactggc cctgccctcc aaatatgtga
     3841 acttcctgta cctggccagc cactatgaga agctgaaggg ctcccccgag gataatgagc
     3901 agaaacagct gtttgtggaa cagcacaaac actacctgga cgagatcatc gagcagatca
     3961 gcgagttctc caagagagtg atcctggccg acgctaatct ggacaaggtg ctgagcgcct
     4021 acaacaagca cagagacaag cctatcagag agcaggccga gaatatcatc cacctgttta
     4081 ccctgaccaa tctgggagcc cctgccgcct tcaagtactt tgacaccacc atcgaccgga
     4141 agaggtacac cagcaccaaa gaggtgctgg acgccaccct gatccaccag agcatcaccg
     4201 gcctgtacga gacacggatc gacctgtctc agctgggagg cgacgcctat ccctatgacg
     4261 tgcccgatta tgccagcctg ggcagcggct cccccaagaa aaaacgcaag gtggaagatc
     4321 ctaagaaaaa gcggaaagtg gacggcattg gtagtgggag caacggcagc agcggatcca
     4381 acggtccgac tgacgccgcg gaagaggagc ttctgagcaa aaactatcac ctcgaaaacg
     4441 aggttgcgcg actgaagaaa ggaagcgggt ccggtggaag tggctccgga tctggaggtt
     4501 ctggcagcgg aggtagcggc agtggcgaag agctccttag taagaactat catctggaaa
     4561 atgaggtagc gcgcttaaag aaagggtcgg gaagtggcgg cagcggaagt gggagtggag
     4621 ggagcggttc tggcggttcc ggcagtggag aggagttgct gtctaagaac taccacttag
     4681 aaaacgaagt cgcacggcta aaaaaaggtt ccggctccgg cggctccggt tctggaagcg
     4741 ggggctcggg atcaggtgga tctggatcag gagaggaatt gctttccaaa aactaccacc
     4801 ttgagaatga ggtggccagg ttaaagaagg ggagcggctc ggggggtagt ggatcggggt
     4861 cgggcgggtc aggaagcggt ggtagcggat ctggggagga gctgctctcg aagaattacc
     4921 atttggagaa cgaagtggcg agactaaaga agggaagcgg tagtggtggt tcagggtctg
     4981 gttcaggtgg cagtgggtct gggggctcag ggtccgggac ggccggcctc ggaagcggag
     5041 ctactaactt cagcctgctg aagcaggctg gagacgtgga ggagaaccct ggacctagta
     5101 ccatgggccc cgacatcgtg atgacccaga gccccagcag cctgagcgcc agcgtgggcg
     5161 accgcgtgac catcacctgc cgcagcagca ccggcgccgt gaccaccagc aactacgcca
     5221 gctgggtgca ggagaagccc ggcaagctgt tcaagggcct gatcggcggc accaacaacc
     5281 gcgcccccgg cgtgcccagc cgcttcagcg gcagcctgat cggcgacaag gccaccctga
     5341 ccatcagcag cctgcagccc gaggacttcg ccacctactt ctgcgccctg tggtacagca
     5401 accactgggt gttcggccag ggcaccaagg tggagctgaa gcgcggcggc ggcggcagcg
     5461 gcggcggcgg cagcggcggc ggcggcagca gcggcggcgg cagcgaggtg aagctgctgg
     5521 agagcggcgg cggcctggtg cagcccggcg gcagcctgaa gctgagctgc gccgtgagcg
     5581 gcttcagcct gaccgactac ggcgtgaact gggtgcgcca ggcccccggc cgcggcctgg
     5641 agtggatcgg cgtgatctgg ggcgacggca tcaccgacta caacagcgcc ctgaaggacc
     5701 gcttcatcat cagcaaggac aacggcaaga acaccgtgta cctgcagatg agcaaggtgc
     5761 gcagcgacga caccgccctg tactactgcg tgaccggcct gttcgactac tggggccagg
     5821 gcaccctggt gaccgtgagc agctacccat acgatgttcc agattacgct ggtggaggcg
     5881 gaggttctgg gggaggaggt agtggcggtg gtggttcagg aggcggcgga agcttggatc
     5941 caggtggagg tggaagcggt agcaaaggag aagaactttt cactggagtt gtcccaattc
     6001 ttgttgaatt agatggtgat gttaatgggc acaaattttc tgtccgtgga gagggtgaag
     6061 gtgatgctac aaacggaaaa ctcaccctta aatttatttg cactactgga aaactacctg
     6121 ttccgtggcc aacacttgtc actactctga cctatggtgt tcaatgcttt tcccgttatc
     6181 cggatcacat gaaacggcat gactttttca agagtgccat gcccgaaggt tatgtacagg
     6241 aacgcactat atctttcaaa gatgacggga cctacaagac gcgtgctgaa gtcaagtttg
     6301 aaggtgatac ccttgttaat cgtatcgagt taaagggtat tgattttaaa gaagatggaa
     6361 acattcttgg acacaaactc gagtacaact ttaactcaca caatgtatac atcacggcag
     6421 acaaacaaaa gaatggaatc aaagctaact tcaaaattcg ccacaacgtt gaagatggtt
     6481 ccgttcaact agcagaccat tatcaacaaa atactccaat tggcgatggc cctgtccttt
     6541 taccagacaa ccattacctg tcgacacaat ctgtcctttc gaaagatccc aacgaaaagc
     6601 gtgaccacat ggtccttctt gagtttgtaa ctgctgctgg gattacacat ggcatggatg
     6661 agctctacaa aggtggaggt cggaccggtg gcggtggcgg aggggctagc agatccgaac
     6721 tgcccacctg cagctgtctt gatcgagtta tacaaaaaga caaaggccca tattatacac
     6781 accttggggc aggaccaagt gttgctgctg tcagggaaat catggagaat aggtatggtc
     6841 aaaaaggaaa cgcaataagg atagaaatag tagtgtacac cggtaaagaa gggaaaagct
     6901 ctcatgggtg tccaattgct aagtgggttt taagaagaag cagtgatgaa gaaaaagttc
     6961 tttgtttggt ccggcagcgt acaggccacc actgtccaac tgctgtgatg gtggtgctca
     7021 tcatggtgtg ggatggcatc cctcttccaa tggccgaccg gctatacaca gagctcacag
     7081 agaatctaaa gtcatacaat gggcacccta ccgacagaag atgcaccctc aatgaaaatc
     7141 gtacctgtac atgtcaagga attgatccag agacttgtgg agcttcattc tcttttggct
     7201 gttcatggag tatgtacttt aatggctgta agtttggtag aagcccaagc cccagaagat
     7261 ttagaattga tccaagctct cccttacatg aaaaaaacct tgaagataac ttacagagtt
     7321 tggctacacg attagctcca atttataagc agtatgctcc agtagcttac caaaatcagg
     7381 tggaatatga aaatgttgcc cgagaatgtc ggcttggcag caaggaaggt cgtcccttct
     7441 ctggggtcac tgcttgcctg gacttctgtg ctcatcccca cagggacatt cacaacatga
     7501 ataatggaag cactgtggtt tgtaccttaa ctcgagaaga taaccgctct ttgggtgtta
     7561 ttcctcaaga tgagcagctc catgtgctac ctctttataa gctttcagac acagatgagt
     7621 ttggctccaa ggaaggaatg gaagccaaga tcaaatctgg ggccatcgag gtcctggcac
     7681 cccgccgcaa aaaaagaacg tgtttcactc agcctgttcc ccgttctgga aagaagaggg
     7741 ctgcgatgat gacagaggtt cttgcacata agataagggc agtggaaaag aaacctattc
     7801 cccgaatcaa gcggaagaat aactcaacaa caacaaacaa cagtaagcct tcgtcactgc
     7861 caaccttagg gagtaacact gagaccgtgc aacctgaagt aaaaagtgaa accgaacccc
     7921 attttatctt aaaaagttca gacaacacta aaacttattc gctgatgcca tccgctcctc
     7981 acccagtgaa agaggcatct ccaggcttct cctggtcccc gaagactgct tcagccacac
     8041 cagctccact gaagaatgac gcaacagcct catgcgggtt ttcagaaaga agcagcactc
     8101 cccactgtac gatgccttcg ggaagactca gtggtgccaa tgcagctgct gctgatggcc
     8161 ctggcatttc acagcttggc gaagtggctc ctctccccac cctgtctgct cctgtgatgg
     8221 agcccctcat taattctgag ccttccactg gtgtgactga gccgctaacg cctcatcagc
     8281 caaaccacca gccctccttc ctcacctctc ctcaagacct tgcctcttct ccaatggaag
     8341 aagatgagca gcattctgaa gcagatgagc ctccatcaga cgaaccccta tctgatgacc
     8401 ccctgtcacc tgctgaggag aaattgcccc acattgatga gtattggtca gacagtgagc
     8461 acatcttttt ggatgcaaat attggtgggg tggccatcgc acctgctcac ggctcggttt
     8521 tgattgagtg tgcccggcga gagctgcacg ctaccactcc tgttgagcac cccaaccgta
     8581 atcatccaac ccgcctctcc cttgtctttt accagcacaa aaacctaaat aagccccaac
     8641 atggttttga actaaacaag attaagtttg aggctaaaga agctaagaat aagaaaatga
     8701 aggcctcaga gcaaaaagac caggcagcta atgaaggtcc agaacagtcc tctgaagtaa
     8761 atgaattgaa ccaaattcct tctcataaag cattaacatt aacccatgac aatgttgtca
     8821 ccgtgtcccc ttatgctctc acacacgttg cggggcccta taaccattgg gtctgagcgg
     8881 ccgcacccag ctttcttgta caaagtggcc ccgacctgca ggcatgcagt taacaatcaa
     8941 cctctggatt acaaaatttg tgaaagattg actggtattc ttaactatgt tgctcctttt
     9001 acgctatgtg gatacgctgc tttaatgcct ttgtatcatg ctattgcttc ccgtatggct
     9061 ttcattttct cctccttgta taaatcctgg ttgctgtctc tttatgagga gttgtggccc
     9121 gttgtcaggc aacgtggcgt ggtgtgcact gtgtttgctg acgcaacccc cactggttgg
     9181 ggcattgcca ccacctgtca gctcctttcc gggactttcg ctttccccct ccctattgcc
     9241 acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg gctgttgggc
     9301 actgacaatt ccgtggtgtt gtcggggaag ctgacgtcct ttccatggct gctcgcctgt
     9361 gttgccacct ggattctgcg cgggacgtcc ttctgctacg tcccttcggc cctcaatcca
     9421 gcggaccttc cttcccgcgg cctgctgccg gctctgcggc ctcttccgcg tccttcgcct
     9481 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgaga
     9541 cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg ctgattgtgc
     9601 ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc aggtaccttt
     9661 aagaccaatg acttacaagg cagctgtaga tctctagata actgatcata atcagccata
     9721 ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga
     9781 aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca
     9841 aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt
     9901 gtggtttgtc caaactcatc aatgtatctt aacgcgctag caaaaagctt taatgcggta
     9961 gtttatcaca gttaaattgc taacgcagtc aggcaccgtg tatgaaatct aacaatgcgc
    10021 tcatcgtcat cctcggcacc gtcaccctgg atgctgtagg cataggcttg gttatgccgg
    10081 tactgccggg cctcttgcgg ggaattctac cgggtagggg aggcgctttt cccaaggcag
    10141 tctggagcat gcgctttagc agccccgctg ggcacttggc gctacacaag tggcctctgg
    10201 cctcgcacac attccacatc caccggtagg cgccaaccgg ctccgttctt tggtggcccc
    10261 ttcgcgccac cttctactcc tcccctagtc aggaagttcc cccccgcccc gcagctcgcg
    10321 tcgtgcagga cgtgacaaat ggaagtagca cgtctcacta gtctcgtgca gatggacagc
    10381 accgctgagc aatggaagcg ggtaggcctt tggggcagcg gccaatagca gctttgctcc
    10441 ttcgctttct gggctcagag gctgggaagg ggtgggtccc ggggcgggct caggggcggg
    10501 ctcaggggcg gggcgggcgc ccgaaggtcc tccggaggcc cggcattctg cacgcttcaa
    10561 aagcgcacgt ctgccgcgct gttctcctct tcctcatctc cgggcctttc gaccgatcat
    10621 caagcttgat cctcatgacc gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg
    10681 tccccagggc cgtacgcacc ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca
    10741 ccgtcgatcc ggaccgccac atcgagcggg tcaccgagct gcaagaactc ttcctcacgc
    10801 gcgtcgggct cgacatcggc aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct
    10861 ggaccacgcc ggagagcgtc gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg
    10921 ccgagttgag cggttcccgg ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc
    10981 accggcccaa ggagcccgcg tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg
    11041 gcaagggtct gggcagcgcc gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg
    11101 tgcccgcctt cctggagacc tccgcgcccc gcaacctccc cttctacgag cggctcggct
    11161 tcaccgtcac cgccgacgtc gaggtgcccg aaggaccgcg cacctggtgc atgacccgca
    11221 agcccggtgc cggatccatg cccacgctac tgcgggttta tatagacggt ccccacggga
    11281 tggggaaaac caccaccacg caactgctgg tggccctggg ttcgcgcgac gatatcgtct
    11341 acgtacccga gccgatgact tactggcggg tgctgggggc ttccgagaca atcgcgaaca
    11401 tctacaccac acaacaccgc ctcgaccagg gtgagatatc ggccggggac gcggcggtgg
    11461 taatgacaag cgcccagata acaatgggca tgccttatgc cgtgaccgac gccgttctgg
    11521 ctcctcatat cgggggggag gctgggagct cacatgcccc gcccccggcc ctcaccctca
    11581 tcttcgaccg ccatcccatc gccgccctcc tgtgctaccc ggccgcgcgg taccttatgg
    11641 gcagcatgac cccccaggcc gtgctggcgt tcgtggccct catcccgccg accttgcccg
    11701 gcaccaacat cgtgcttggg gcccttccgg aggacagaca catcgaccgc ctggccaaac
    11761 gccagcgccc cggcgagcgg ctggacctgg ctatgctggc tgcgattcgc cgcgtttacg
    11821 ggctacttgc caatacggtg cggtatctgc agtgcggcgg gtcgtggcgg gaggactggg
    11881 gacagctttc ggggacggcc gtgccgcccc agggtgccga gccccagagc aacgcgggcc
    11941 cacgacccca tatcggggac acgttattta ccctgtttcg ggcccccgag ttgctggccc
    12001 ccaacggcga cctgtataac gtgtttgcct gggccttgga cgtcttggcc aaacgcctcc
    12061 gttccatgca cgtctttatc ctggattacg accaatcgcc cgccggctgc cgggacgccc
    12121 tgctgcaact tacctccggg atggtccaga cccacgtcac cacccccggc tccataccga
    12181 cgatatgcga cctggcgcgc acgtttgccc gggagatggg ggaggctaac tgagctctag
    12241 cttatcgact cgccgatagt ggaaaccgac gccccagcac tcgtccgagg gcaaaggaat
    12301 agtcgagaaa ttgatgatct attaaacaat aaagatgtcc acatggaagt ttttcctgtc
    12361 atactttgtt aagaagggtg agaacagagt acctacattt tgaatggaag gattggagct
    12421 acgggggtgg gggtggggtg ggattagata aatgcctgct ctttactgaa ggctctttac
    12481 tattgcttta tgataatgtt tcatagttgg atatcataat ttaaacaagc aaaaccaaat
    12541 taagggccag ctcattcctc ccactcatga tctatagatc catcagatcc tttgttactt
    12601 tatagaagaa attttgagtt tttgtttttt tttaataaat aaataaacat aaataaattg
    12661 tttgttgaat ttattattag tatgtaagtg taaatataat aaaacttaat atctattcaa
    12721 attaataaat aaacctcgat atacagaccg ataaaacaca tgcgtcaatt ttacgcatga
    12781 ttatctttaa cgtacgtcac aatatgatta tctttctagg gttaaagagc tccagctttt
    12841 gttcccttta gtgagggtta atttcgagct tggcgtaatc atggtcatag ctgtttcctg
    12901 tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
    12961 aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
    13021 ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
    13081 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
    13141 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
    13201 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
    13261 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
    13321 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
    13381 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
    13441 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
    13501 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
    13561 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
    13621 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
    13681 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca gtatttggta
    13741 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
    13801 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
    13861 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
    13921 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
    13981 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
    14041 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
    14101 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
    14161 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
    14221 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
    14281 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
    14341 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
    14401 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
    14461 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
    14521 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
    14581 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
    14641 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
    14701 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
    14761 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
    14821 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
    14881 cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
    14941 agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
    15001 gggttccgcg cacatttccc cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt
    15061 aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg
    15121 caaaatccct tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg
    15181 gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta
    15241 tcagggcgat ggcccactac gtgaaccatc accctaatca agttttttgg ggtcgaggtg
    15301 ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa
    15361 gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct
    15421 ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct
    15481 acagggcgcg tcccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    15541 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    15601 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aattgtaata
    15661 cgactcacta tagggcgaat tgggtacctt aaccctagaa agatagtctg cgtaaaattg
    15721 acgcatgcat tcttgaaata ttgctctctc tttctaaata gcgcgaatcc gtcgctgtgc
    15781 atttaggaca tctcagtcgc cgcttggagc tcccgtgagg cgtgcttgtc aatgcggtaa
    15841 gtgtcactga ttttgaacta taacgaccgc gtgagtcaaa atgacgcatg attatctttt
    15901 acgtgacttt taagatttaa ctcatacgat aattatattg ttatttcatg ttctacttac
    15961 gtgataactt attatatata tattttcttg ttatagatat cgtcgacatt gattattgac
    16021 tagctagtta ttaatagtaa tcaattacgg ggtcattagt tcatagccca tatatggagt
    16081 tccgcgttac ataacttacg gtaaatggcc cgcctggctg accgcccaac gacccccgcc
    16141 cattgacgtc aataatgacg tatgttccca tagtaacgcc aatagggact ttccattgac
    16201 gtcaatgggt ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata
    16261 tgccaagtac gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattatgccc
    16321 agtacatgac cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta
    16381 ttaccatgat ggtcgaggtg agccccacgt tctgcttcac tctccccatc tcccccccct
    16441 ccccaccccc aattttgtat ttatttattt tttaattatt ttgtgcagcg atgggggcgg
    16501 gggggggggg ggggcgcgcg ccaggcgggg cggggcgggg cgaggggcgg ggcggggcga
    16561 ggcggagagg tgcggcggca gccaatcaga gcggcgcgct ccgaaagttt ccttttatgg
    16621 cgaggcggcg gcggcggcgg ccctataaaa agcgaagcgc gcggcgggcg ggagtcgctg
    16681 cgcgctgcct tcgccccgtg ccccgctccg ccgccgcctc gcgccgcccg ccccggctct
    16741 gactgaccgc gttactccca caggtgagcg ggcgggacgg cccttctcct ccgggctgta
    16801 attagcgctt ggtttaatga cggcttgttt cttttctgtg gctgcgtgaa agccttgagg
    16861 ggctccggga gggccctttg tgcgggggga gcggctcggg gggtgcgtgc gtgtgtgtgt
    16921 gcgtggggag cgccgcgtgc ggctccgcgc tgcccggcgg ctgtgagcgc tgcgggcgcg
    16981 gcgcggggct ttgtgcgctc cgcagtgtgc gcgaggggag cgcggccggg ggcggtgccc
    17041 cgcggtgcgg ggggggctgc gaggggaaca aaggctgcgt gcggggtgtg tgcgtggggg
    17101 ggtgagcagg gggtgtgggc gcgtcggtcg ggctgcaacc ccccctgcac ccccctcccc
    17161 gagttgctga gcacggcccg gcttcgggtg cggggctccg tacggggcgt ggcgcggggc
    17221 tcgccgtgcc gggcgggggg tggcggcagg tgggggtgcc gggcggggcg gggccgcctc
    17281 gggccgggga gggctcgggg gaggggcgcg gcggcccccg gagcgccggc ggctgtcgag
    17341 gcgcggcgag ccgcagccat tgccttttat ggtaatcgtg cgagagggcg cagggacttc
    17401 ctttgtccca aatctgtgcg gagccgaaat ctgggaggcg ccgccgcacc ccctctagcg
    17461 ggcgcggggc gaagcggtgc ggcgccggca ggaaggaaat gggcggggag ggccttcgtg
    17521 cgtcgccgcg ccgccgtccc cttctccctc tccagcctcg gggctgtccg cggggggacg
    17581 gctgccttcg ggggggacgg ggcagggcgg ggttcggctt ctggcgtgtg accggcggct
    17641 ctagagcctc tgctaaccat gttcatgcct tcttcttttt cctacagctc ctgggcaacg
    17701 tgctggttat tgtgctgtct catcattttg gcaaagaatt cc
//
