LOCUS       sequence-424241-       11369 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Mammalian continuous expression.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11369)
  TITLE     Yoshimatsu Lab plasmids 2023
REFERENCE   2  (bases 1 to 11369)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: lentiCherry U6-sgRNA (BsmBI)
FEATURES             Location/Qualifiers
     source          1..11369
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..404
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        406..604
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     559..579
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             622..802
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    849..974
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1467..1700
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1885..1929
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2227..2344
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     promoter        2395..2643
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     2395..2415
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     2566..2585
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        4525..4600
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     promoter        4662..4873
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     regulatory      4886..4895
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             4892..5569
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="MASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAK
                     LKVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGV
                     VTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKM
                     RLKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHS
                     TGA"
     CDS             5579..5635
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             5636..6232
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="TEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIERV
                     TELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLAA
                     QQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETSA
                     PRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     6129..6149
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    6248..6836
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6301..6321)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6719..6730)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6908..7141
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     primer_bind     complement(7167..7184)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7173..7397
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7443..7871
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7530..7549)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7740..7761
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7880..7900)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7885..8214
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8065..8200
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8127..8146
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8262..8309
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8328..8702
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8832..8953
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8869..8888)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8923..8942
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9002..9018)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9002..9018)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9015..9037)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9026..9042
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9050..9056,9057..9074,9075..9080))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9095..9116
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9233..9250)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9404..9992)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9484..9503)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10163..10954,10955..11023))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10786..10805
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11024..11128)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11203..11222)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
ORIGIN
        1 atgtacgggc cagatatacg cgttgacatt gattattgac tagttattaa tagtaatcaa
       61 ttacggggtc attagttcat agcccatata tggagttccg cgttacataa cttacggtaa
      121 atggcccgcc tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg
      181 ttcccatagt aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt
      241 aaactgccca cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg
      301 tcaatgacgg taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc
      361 ctacttggca gtacatctac gtattagtca tcgctattac catggtgatg cggttttggc
      421 agtacatcaa tgggcgtgga tagcggtttg actcacgggg atttccaagt ctccacccca
      481 ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg ggactttcca aaatgtcgta
      541 acaactccgc cccattgacg caaatgggcg gtaggcgtgt acggtgggag gtctatataa
      601 gcagcgcgtt ttgcctgtac tgggtctctc tggttagacc agatctgagc ctgggagctc
      661 tctggctaac tagggaaccc actgcttaag cctcaataaa gcttgccttg agtgcttcaa
      721 gtagtgtgtg cccgtctgtt gtgtgactct ggtaactaga gatccctcag acccttttag
      781 tcagtgtgga aaatctctag cagtggcgcc cgaacaggga cttgaaagcg aaagggaaac
      841 cagaggagct ctctcgacgc aggactcggc ttgctgaagc gcgcacggca agaggcgagg
      901 ggcggcgact ggtgagtacg ccaaaaattt tgactagcgg aggctagaag gagagagatg
      961 ggtgcgagag cgtcagtatt aagcggggga gaattagatc gcgatgggaa aaaattcggt
     1021 taaggccagg gggaaagaaa aaatataaat taaaacatat agtatgggca agcagggagc
     1081 tagaacgatt cgcagttaat cctggcctgt tagaaacatc agaaggctgt agacaaatac
     1141 tgggacagct acaaccatcc cttcagacag gatcagaaga acttagatca ttatataata
     1201 cagtagcaac cctctattgt gtgcatcaaa ggatagagat aaaagacacc aaggaagctt
     1261 tagacaagat agaggaagag caaaacaaaa gtaagaccac cgcacagcaa gcggccgctg
     1321 atcttcagac ctggaggagg agatatgagg gacaattgga gaagtgaatt atataaatat
     1381 aaagtagtaa aaattgaacc attaggagta gcacccacca aggcaaagag aagagtggtg
     1441 cagagagaaa aaagagcagt gggaatagga gctttgttcc ttgggttctt gggagcagca
     1501 ggaagcacta tgggcgcagc gtcaatgacg ctgacggtac aggccagaca attattgtct
     1561 ggtatagtgc agcagcagaa caatttgctg agggctattg aggcgcaaca gcatctgttg
     1621 caactcacag tctggggcat caagcagctc caggcaagaa tcctggctgt ggaaagatac
     1681 ctaaaggatc aacagctcct ggggatttgg ggttgctctg gaaaactcat ttgcaccact
     1741 gctgtgcctt ggaatgctag ttggagtaat aaatctctgg aacagatttg gaatcacacg
     1801 acctggatgg agtgggacag agaaattaac aattacacaa gcttaataca ctccttaatt
     1861 gaagaatcgc aaaaccagca agaaaagaat gaacaagaat tattggaatt agataaatgg
     1921 gcaagtttgt ggaattggtt taacataaca aattggctgt ggtatataaa attattcata
     1981 atgatagtag gaggcttggt aggtttaaga atagtttttg ctgtactttc tatagtgaat
     2041 agagttaggc agggatattc accattatcg tttcagaccc acctcccaac cccgagggga
     2101 cccgacaggc ccgaaggaat agaagaagaa ggtggagaga gagacagaga cagatccatt
     2161 cgattagtga acggatcggc actgcgtgcg ccaattctgc agacaaatgg cagtattcat
     2221 ccacaatttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     2281 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aaattcaaaa
     2341 ttttcgggtt tattacaggg acagcagaga tccagtttgg ttaattaagg taccgagggc
     2401 ctatttccca tgattccttc atatttgcat atacgataca aggctgttag agagataatt
     2461 agaattaatt tgactgtaaa cacaaagata ttagtacaaa atacgtgacg tagaaagtaa
     2521 taatttcttg ggtagtttgc agttttaaaa ttatgtttta aaatggacta tcatatgctt
     2581 accgtaactt gaaagtattt cgatttcttg gctttatata tcttgtggaa aggacgaaac
     2641 accggagacg gttgtaaatg agcacacaaa atacacatgc taaaatatta tattctatga
     2701 cctttataaa atcaaccaaa atcttctttt taataacttt agtatcaata attagaattt
     2761 ttatgttcct ttttgcaaac ttttaataaa aatgagcaaa ataaaaaaac gctagtttta
     2821 gtaactcgcg ttgttttctt cacctttaat aatagctact ccaccacttg ttcctaagcg
     2881 gtcagctcct gcttcaatca ttttttgagc atcttcaaat gttctaactc caccagctgc
     2941 tttaactaaa gcattgtctt taacaactga cttcattagt ttaacatctt caaatgttgc
     3001 acctgatttt gaaaatcctg ttgatgtttt aacaaattct aatccagctt caacagctat
     3061 ttcacaagct ttcatgattt cttcttttgt taataaacaa ttttccataa tacatttaac
     3121 aacatgtgat ccagctgctt tttttacagc tttcatgtct tctaaaacta attcataatt
     3181 tttgtctttt aatgcaccaa tatttaatac catatcaatt tctgttgcac catctttaat
     3241 tgcttcagaa acttcgaatg cttttgtagc tgttgtgcat gcacctagag gaaaacctac
     3301 aacatttgtt attcctacat ttgtgccttt taataattct ttacaatagc ttgttcaata
     3361 tgaattaaca caaactgttg caaaatcaaa ttcaattgct tcatcacata attgtttaat
     3421 ttcagctttc gtagcatctt gttttaataa tgtgtgatct atatatttgt ttagtttcat
     3481 tttttctcct atatattcat ttttaatttt aattctttaa taatttcgtc tactttaact
     3541 ttagcgtttt gaacagattc accaacacct ataaaataaa tttttagttt aggttcagtt
     3601 ccacttgggc gaacagcaaa tcatgactta tcttctaaat aaaattttag taagtcttgt
     3661 cctggcatat tatacattcc atcgatgtag tcttcaacat taacaacttt aagtccagca
     3721 atttgagtta agggtgttgc tctcaatgat ttcattaatg gttcaatttt taatttcttt
     3781 tcttctggtt taaaattcaa gtttaaagtg aaagtgtaat atgcacccat ttctttaaat
     3841 aaatcttcta aatagtctac taatgtttta ttttgttttt tataaaatca agcagcctct
     3901 gctattaata tagaagcttg tattccatct ttatctctag ctgagtcatc aattacatat
     3961 ccataacttt cttcataagc aaaaacaaaa tttaatccgt tatcttcttc tttagcaatt
     4021 tctctaccca ttcatttaaa tccagttaaa gtttttacaa tattaactcc atatttttca
     4081 tgagcgattc tatcacccaa atcacttgtt acaaaacttg aatatagagc cggatttttt
     4141 ggaatgctat ttaagcgttt tagatttgat aattttcaat caattaaaat tggtcctgtt
     4201 tgatttccat ctaatcttac aaaatgacca tcatgtttta ttgccattcc aaatctgtca
     4261 gcatctgggt cattcataat aataatatct gcatcatgtt taataccata ttcaagcggt
     4321 atttttcatg caggatcaaa ttctggattt ggatttacaa catttttaaa tgtttcatct
     4381 tcaaatgcat gctcttcaac ctcaataacg ttatatcctg attcacgtaa tatttttggg
     4441 gtaaatttag ttcctgttcc attaactgcg ctaaaaataa tttttaaatc ttttttagct
     4501 tcttgctctt ttttgtacgt ctctgtttta gagctagaaa tagcaagtta aaataaggct
     4561 agtccgttat caacttgaaa aagtggcacc gagtcggtgc ttttttgaat tcgctagcta
     4621 ggtcttgaaa ggagtgggaa ttggctccgg tgcccgtcag tgggcagagc gcacatcgcc
     4681 cacagtcccc gagaagttgg ggggaggggt cggcaattga tccggtgcct agagaaggtg
     4741 gcgcggggta aactgggaaa gtgatgtcgt gtactggctc cgcctttttc ccgagggtgg
     4801 gggagaaccg tatataagtg cagtagtcgc cgtgaacgtt ctttttcgca acgggtttgc
     4861 cgccagaaca caggaccggt ctagagccac catggcgagt agcgaagacg ttatcaaaga
     4921 gttcatgcgt ttcaaagttc gtatggaagg ttccgttaac ggtcacgagt tcgaaatcga
     4981 aggtgaaggt gaaggtcgtc cgtacgaagg tacccagacc gctaaactga aagttaccaa
     5041 aggtggtccg ctgccgttcg cttgggacat cctgtccccg cagttccagt acggttccaa
     5101 agcttacgtt aaacacccgg ctgacatccc ggactacctg aaactgtcct tcccggaagg
     5161 tttcaaatgg gaacgtgtta tgaacttcga agacggtggt gttgttaccg ttacccagga
     5221 ctcctccctg caagacggtg agttcatcta caaagttaaa ctgcgtggta ccaacttccc
     5281 gtccgacggt ccggttatgc agaaaaaaac catgggttgg gaagcttcca ccgaacgtat
     5341 gtacccggaa gacggtgctc tgaaaggtga aatcaaaatg cgtctgaaac tgaaagacgg
     5401 tggtcactac gacgctgaag ttaaaaccac ctacatggct aaaaaaccgg ttcagctgcc
     5461 gggtgcttac aaaaccgaca tcaaactgga catcacctcc cacaacgaag actacaccat
     5521 cgttgaacag tacgaacgtg ctgaaggtcg tcactccacc ggtgcttaag gatccggcgc
     5581 aacaaacttc tctctgctga aacaagccgg agatgtcgaa gagaatcctg gaccgaccga
     5641 gtacaagccc acggtgcgcc tcgccacccg cgacgacgtc cccagggccg tacgcaccct
     5701 cgccgccgcg ttcgccgact accccgccac gcgccacacc gtcgatccgg accgccacat
     5761 cgagcgggtc accgagctgc aagaactctt cctcacgcgc gtcgggctcg acatcggcaa
     5821 ggtgtgggtc gcggacgacg gcgccgcggt ggcggtctgg accacgccgg agagcgtcga
     5881 agcgggggcg gtgttcgccg agatcggccc gcgcatggcc gagttgagcg gttcccggct
     5941 ggccgcgcag caacagatgg aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg
     6001 gttcctggcc accgtcggag tctcgcccga ccaccagggc aagggtctgg gcagcgccgt
     6061 cgtgctcccc ggagtggagg cggccgagcg cgccggggtg cccgccttcc tggagacctc
     6121 cgcgccccgc aacctcccct tctacgagcg gctcggcttc accgtcaccg ccgacgtcga
     6181 ggtgcccgaa ggaccgcgca cctggtgcat gacccgcaag cccggtgcct gaacgcgtta
     6241 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     6301 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     6361 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     6421 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     6481 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     6541 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     6601 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg
     6661 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     6721 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     6781 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcgtcg
     6841 actttaagac caatgactta caaggcagct gtagatctta gccacttttt aaaagaaaag
     6901 gggggactgg aagggctaat tcactcccaa cgaagacaag atctgctttt tgcttgtact
     6961 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     7021 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     7081 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     7141 agggcccgtt taaacccgct gatcagcctc gactgtgcct tctagttgcc agccatctgt
     7201 tgtttgcccc tcccccgtgc cttccttgac cctggaaggt gccactccca ctgtcctttc
     7261 ctaataaaat gaggaaattg catcgcattg tctgagtagg tgtcattcta ttctgggggg
     7321 tggggtgggg caggacagca agggggagga ttgggaagac aatagcaggc atgctgggga
     7381 tgcggtgggc tctatggctt ctgaggcgga aagaaccagc tggggctcta gggggtatcc
     7441 ccacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     7501 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     7561 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     7621 tagtgcttta cggcacctcg accccaaaaa acttgattag ggtgatggtt cacgtagtgg
     7681 gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt tctttaatag
     7741 tggactcttg ttccaaactg gaacaacact caaccctatc tcggtctatt cttttgattt
     7801 ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt aacaaaaatt
     7861 taacgcgaat taattctgtg gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc
     7921 ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
     7981 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     8041 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
     8101 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
     8161 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
     8221 ccgggagctt gtatatccat tttcggatct gatcagcacg tgttgacaat taatcatcgg
     8281 catagtatat cggcatagta taatacgaca aggtgaggaa ctaaaccatg gccaagttga
     8341 ccagtgccgt tccggtgctc accgcgcgcg acgtcgccgg agcggtcgag ttctggaccg
     8401 accggctcgg gttctcccgg gacttcgtgg aggacgactt cgccggtgtg gtccgggacg
     8461 acgtgaccct gttcatcagc gcggtccagg accaggtggt gccggacaac accctggcct
     8521 gggtgtgggt gcgcggcctg gacgagctgt acgccgagtg gtcggaggtc gtgtccacga
     8581 acttccggga cgcctccggg ccggccatga ccgagatcgg cgagcagccg tgggggcggg
     8641 agttcgccct gcgcgacccg gccggcaact gcgtgcactt cgtggccgag gagcaggact
     8701 gacacgtgct acgagatttc gattccaccg ccgccttcta tgaaaggttg ggcttcggaa
     8761 tcgttttccg ggacgccggc tggatgatcc tccagcgcgg ggatctcatg ctggagttct
     8821 tcgcccaccc caacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     8881 caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     8941 tcaatgtatc ttatcatgtc tgtataccgt cgacctctag ctagagcttg gcgtaatcat
     9001 ggtcatagct gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag
     9061 ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg
     9121 cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa
     9181 tcggccaacg cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca
     9241 ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     9301 taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     9361 agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     9421 cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     9481 tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     9541 tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     9601 gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     9661 acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     9721 acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     9781 cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     9841 gaagaacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     9901 gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     9961 agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
    10021 ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
    10081 ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
    10141 atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
    10201 tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
    10261 gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
    10321 ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
    10381 caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
    10441 cgccagttaa tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct
    10501 cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
    10561 cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
    10621 agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
    10681 tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
    10741 agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac
    10801 atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
    10861 ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
    10921 cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
    10981 caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
    11041 attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
    11101 agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtcg
    11161 acggatcggg agatctcccg atcccctatg gtgcactctc agtacaatct gctctgatgc
    11221 cgcatagtta agccagtatc tgctccctgc ttgtgtgttg gaggtcgctg agtagtgcgc
    11281 gagcaaaatt taagctacaa caaggcaagg cttgaccgac aattgcatga agaatctgct
    11341 tagggttagg cgttttgcgc tgcttcgcg
//
