LOCUS       sequence-424260-        1053 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  part B of SPARK-OFF.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1053)
  AUTHORS   Chung CI, Yang J, Yang X, Liu H, Ma Z, Szulzewsky F, Holland EC, 
            Shen Y, Shu X
  TITLE     Phase separation of YAP-MAML2 differentially regulates the 
            transcriptome.
  JOURNAL   Proc Natl Acad Sci U S A. 2024 Feb 13;121(7):e2310430121. doi: 
            10.1073/pnas.2310430121. Epub 2024 Feb 5.
  PUBMED    38315854
REFERENCE   2  (bases 1 to 1053)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2024-02-13"; volume: "121(7):e2310430121. 
            doi"; pages: " 10.1073/pnas.2310430121. Epub 2024 Feb 5"
COMMENT     Sequence Label: Frb-NLS-mCherry
FEATURES             Location/Qualifiers
     source          1..1053
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             4..276
                     /codon_start=1
                     /product="FKBP-rapamycin binding domain of human FRAP"
                     /label=FRB
                     /translation="EMWHEGLEEASRLYFGERNVKGMFEVLEPLHAMMERGPQTLKETS
                     FNQAYGRDLMEAQEWCRKYMKSGNVKDLTQAWDLYYHVFRRISKQL"
     CDS             316..342
                     /codon_start=1
                     /product="nuclear localization signal of human c-Myc 
                     proto-oncogene (Dang and Lee, 1988)"
                     /label=c-myc NLS
                     /translation="PAAKRVKLD"
     CDS             343..1053
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(488..506)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     756..775
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 atggagatgt ggcacgaggg actcgaagag gccagcaggc tgtactttgg cgagaggaac
       61 gtcaagggca tgttcgaagt gctggagccc ctccatgcga tgatggaaag gggcccacag
      121 accctgaagg agaccagctt caaccaggct tacggcaggg acctgatgga ggcacaggaa
      181 tggtgcagga agtacatgaa gagcggcaac gtgaaagacc tgacccaggc gtgggacctc
      241 tactaccacg tgttcaggag gatcagcaag cagctcgagt ccggactcag atctggcagc
      301 ggtggaggcg atatccccgc cgccaagcgg gtgaagctgg acatggtgag caagggcgag
      361 gaggataaca tggccatcat caaggagttc atgcgcttca aggtgcacat ggagggctcc
      421 gtgaacggcc acgagttcga gatcgagggc gagggcgagg gccgccccta cgagggcacc
      481 cagaccgcca agctgaaggt gaccaagggt ggccccctgc ccttcgcctg ggacatcctg
      541 tcccctcagt tcatgtacgg ctccaaggcc tacgtgaagc accccgccga catccccgac
      601 tacttgaagc tgtccttccc cgagggcttc aagtgggagc gcgtgatgaa cttcgaggac
      661 ggcggcgtgg tgaccgtgac ccaggactcc tccctgcagg acggcgagtt catctacaag
      721 gtgaagctgc gcggcaccaa cttcccctcc gacggccccg taatgcagaa gaagaccatg
      781 ggctgggagg cctcctccga gcggatgtac cccgaggacg gcgccctgaa gggcgagatc
      841 aagcagaggc tgaagctgaa ggacggcggc cactacgacg ctgaggtcaa gaccacctac
      901 aaggccaaga agcccgtgca gctgcccggc gcctacaacg tcaacatcaa gttggacatc
      961 acctcccaca acgaggacta caccatcgtg gaacagtacg aacgcgccga gggccgccac
     1021 tccaccggcg gcatggacga gctgtacaag taa
//
