LOCUS       sequence-42431-e         837 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expression of the reporter gene under the control of the 
            MiniPromoter in this plasmid has not been tested.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 837)
  AUTHORS   Portales-Casamar E, Swanson DJ, Liu L, de Leeuw CN, Banks KG, Ho Sui
            SJ, Fulton DL, Ali J, Amirabbasi M, Arenillas DJ, Babyak N, Black 
            SF, Bonaguro RJ, Brauer E, Candido TR, Castellarin M, Chen J, Chen 
            Y, Cheng JC, Chopra V, Docking TR, Dreolini L, D'Souza CA, Flynn EK,
            Glenn R, Hatakka K, Hearty TG, Imanian B, Jiang S, Khorasan-zadeh S,
            Komljenovic I, Laprise S, Liao NY, Lim JS, Lithwick S, Liu F, Liu J,
            Lu M, McConechy M, McLeod AJ, Milisavljevic M, Mis J, O'Connor K, 
            Palma B, Palmquist DL, Schmouth JF, Swanson MI, Tam B, Ticoll A, 
            Turner JL, Varhol R, Vermeulen J, Watkins RF, Wilson G, Wong BK, 
            Wong SH, Wong TY, Yang GS, Ypsilanti AR, Jones SJ, Holt RA, 
            Goldowitz D, Wasserman WW, Simpson EM
  TITLE     A regulatory toolbox of MiniPromoters to drive selective expression 
            in the brain.
  JOURNAL   Proc Natl Acad Sci U S A. 2010 Sep 21. 107(38):16589-94.
  PUBMED    20807748
REFERENCE   2  (bases 1 to 837)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A. 2010 Sep 21. 107(38):16589-94."
COMMENT     Sequence Label: pEMS1181
FEATURES             Location/Qualifiers
     source          1..837
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      510..519
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(561..582)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ggcgtatcac gaggcccttt cgtcttcaag aattaattcc gatcatattc aataaccctt
       61 aatgaattcg agctcctagg ccggcctcaa actgtacaca aaccatacac agcagccagc
      121 tcgggtgctg ttaggaaatg gtctcactgc tgggtctgtg gggtatgtgt gtgtctgggt
      181 gtgtggctac tgtctgcatc ctcctccccc ctacagcctc cccgcctccc ctccagccac
      241 cctgggattg gtgactctca gcccctcccc tcagctcccc tagaccctcc cagagccttt
      301 atcagggagc tgggactgag tgactgcagc cttcctagat cccctccact cggtttctct
      361 ctttgcagga gcaccggcag caccagtgtg tgaggggagc aggcagcggt cctagccagt
      421 tccttgatcc tgccagacca cccagccccc ggcacagagc tgctccggcg cgccgaagtt
      481 cctattcttc aaaaggtata ggaacttccg ccaccatggt gagcaagggc gaggagctgt
      541 tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca
      601 gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct
      661 gcaccaccgg caagctgccc gtgccctggc ccaccctcgt gaccaccctg acctacggcg
      721 tgcagtgctt cagccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca
      781 tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactaca
//
