LOCUS       sequence-424311-       10429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human PHGDH.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10429)
  AUTHORS   Wu YQ, Zhang CS, Xiong J, Cai DQ, Wang CZ, Wang Y, Liu YH, Wang Y, 
            Li Y, Wu J, Wu J, Lan B, Wang X, Chen S, Cao X, Wei X, Hu HH, Guo H,
            Yu Y, Ghafoor A, Xie C, Wu Y, Xu Z, Zhang C, Zhu M, Huang X, Sun X, 
            Lin SY, Piao HL, Zhou J, Lin SC
  TITLE     Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine
            synthesis to p53 activation to control cell fate.
  JOURNAL   Cell Res. 2023 Nov;33(11):835-850. doi: 10.1038/s41422-023-00874-4. 
            Epub 2023 Sep 19.
  PUBMED    37726403
REFERENCE   2  (bases 1 to 10429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41422-023-00874-4"; journalName: "Cell Res"; date: 
            "2023-11"; volume: "33"; issue: "11"; pages: "835-850"
COMMENT     Sequence Label: pBOBI C-FLAG PHGDH V261M
FEATURES             Location/Qualifiers
     source          1..10429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        189..387
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             405..585
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    632..757
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1250..1483
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1668..1712
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1964..2080
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2103..2406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2407..2610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2607..2631
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          4403..4632
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     4615..4634
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    4809..5397
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(4862..4882)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5280..5291)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5804..5984
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6010..6027)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6016..6240
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      6286..6714
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6373..6392)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     6583..6604
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(6723..6743)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        6728..7057
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      6908..7043
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     6970..6989
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        7105..7152
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             7171..7545
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    7675..7796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(7712..7731)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     7766..7785
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(7845..7861)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7845..7861)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7858..7880)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7869..7885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7893..7899,7900..7917,7918..7923))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7938..7959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8076..8093)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8247..8835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8327..8346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9006..9797,9798..9866))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9629..9648
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9867..9971)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10046..10065)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(10237..10429,1..187)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
       61 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      121 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      181 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      241 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      301 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      361 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      421 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      481 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      541 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      601 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      661 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      721 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      781 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      841 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      901 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      961 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1021 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1081 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1141 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1201 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1261 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1321 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1381 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1441 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1501 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1561 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1621 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1681 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1741 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1801 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1861 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1921 agagagacag agacagatcc attcgattag tgaacggatc aacttttaaa agaaaagggg
     1981 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2041 ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgataagc ttgggagttc
     2101 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2161 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2221 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2281 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2341 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2401 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2461 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2521 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2581 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     2641 cgccatccac gctgttttga cctccataga agacaccgac tgagctcctt aaggttaact
     2701 ctagagaatt cggatccatg gcttttgcaa atctgcggaa agtgctcatc agtgacagcc
     2761 tggacccttg ctgccggaag atcttgcaag atggagggct gcaggtggtg gaaaagcaga
     2821 accttagcaa agaggagctg atagcggagc tgcaggactg tgaaggcctt attgttcgct
     2881 ctgccaccaa ggtgaccgct gatgtcatca acgcagctga gaaactccag gtggtgggca
     2941 gggctggcac aggtgtggac aatgtggatc tggaggccgc aacaaggaag ggcatcttgg
     3001 ttatgaacac ccccaatggg aacagcctca gtgccgcaga actcacttgt ggaatgatca
     3061 tgtgcctggc caggcagatt ccccaggcga cggcttcgat gaaggacggc aaatgggagc
     3121 ggaagaagtt catgggaaca gagctgaatg gaaagaccct gggaattctt ggcctgggca
     3181 ggattgggag agaggtagct acccggatgc agtcctttgg gatgaagact atagggtatg
     3241 accccatcat ttccccagag gtctcggcct cctttggtgt tcagcagctg cccctggagg
     3301 agatctggcc tctctgtgat ttcatcactg tgcacactcc tctcctgccc tccacgacag
     3361 gcttgctgaa tgacaacacc tttgcccagt gcaagaaggg ggtgcgtgtg gtgaactgtg
     3421 cccgtggagg gatcgtggac gaaggcgccc tgctccgggc cctgcagtct ggccagtgtg
     3481 ccggggctgc actggacatg tttacggaag agccgccacg ggaccgggcc ttggtggacc
     3541 atgagaatgt catcagctgt ccccacctgg gtgccagcac caaggaggct cagagccgct
     3601 gtggggagga aattgctgtt cagttcgtgg acatggtgaa ggggaaatct ctcacggggg
     3661 ttgtgaatgc ccaggccctt accagtgcct tctctccaca caccaagcct tggattggtc
     3721 tggcagaagc tctggggaca ctgatgcgag cctgggctgg gtcccccaaa gggaccatcc
     3781 aggtgataac acagggaaca tccctgaaga atgctgggaa ctgcctaagc cccgcagtca
     3841 ttgtcggcct cctgaaagag gcttccaagc aggcggatgt gaacttggtg aacgctaagc
     3901 tgctggtgaa agaggctggc ctcaatgtca ccacctccca cagccctgct gcaccagggg
     3961 agcaaggctt cggggaatgc ctcctggccg tggccctggc aggcgcccct taccaggctg
     4021 tgggcttggt ccaaggcact acacctgtac tgcaggggct caatggagct gtcttcaggc
     4081 cagaagtgcc tctccgcagg gacctgcccc tgctcctatt ccggactcag acctctgacc
     4141 ctgcaatgct gcctaccatg attggcctcc tggcagaggc aggcgtgcgg ctgctgtcct
     4201 accagacttc actggtgtca gatggggaga cctggcacgt catgggcatc tcctccttgc
     4261 tgcccagcct ggaagcgtgg aagcagcatg tgactgaagc cttccagttc cacttcctcg
     4321 agccatggaa gcttgatatc taactgactg aaccggtggt accgaggaat taattcgctg
     4381 tctgcgaggg ccagctgttg gggtgagtac tccctctcaa aagcgggcat gacttctgcg
     4441 ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc cgcggtgatg
     4501 cctttgaggg tggccgcgtc catctggtca gaaaagacaa tctttttgtt gtcaagcttg
     4561 aggtgtggca ggcttgagat ctggccatac acttgagtga caatgacatc cactttgcct
     4621 ttctctccac aggtgtccac tcccaggtcc aactgcaggt cgagcatgca tctagggcgg
     4681 ccaattccgc cgatcctgga aaaacatgga gcaatcacaa gtagcaatac agcagctacc
     4741 aatgctgatt gtgcctggct agaagcacaa gaggaggagg aggtgggttt tccagtcaca
     4801 cctcagacaa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
     4861 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
     4921 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
     4981 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
     5041 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
     5101 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
     5161 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
     5221 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
     5281 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
     5341 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcctg
     5401 gaattcgagc tcggtacctt taagaccaat gacttacaag gcagctgtag atcttagcca
     5461 ctttttaaaa gaaaaggggg gactggaagg gctaattcac tcccaacgaa gacaagatat
     5521 ccttgatctg tggatctacc acacacaagg ctacttccct gattggcaga actacacacc
     5581 agggccaggg atcagatatc cactgacctt tggatggtgc tacaagctag taccagttga
     5641 gcaagagaag gtagaagaag ccaatgaagg agagaacacc cgcttgttac accctgtgag
     5701 cctgcatggg atggatgacc cggagagaga agtattagag tggaggtttg acagccgcct
     5761 agcatttcat cacatggccc gagagctgca tccggactgt actgggtctc tctggttaga
     5821 ccagatctga gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata
     5881 aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta
     5941 gagatccctc agaccctttt agtcagtgtg gaaaatctct agcagggccg ctttaaaccc
     6001 gctgatcagc ctcgactgtg ccttctagtt gccagccatc tgttgtttgc ccctcccccg
     6061 tgccttcctt gaccctggaa ggtgccactc ccactgtcct ttcctaataa aatgaggaaa
     6121 ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg gggtggggtg gggcaggaca
     6181 gcaaggggga ggattgggaa gacaatagca ggcatgctgg ggatgcggtg ggctctatgg
     6241 cttctgaggc ggaaagaacc agctggggct ctagggggta tccccacgcg ccctgtagcg
     6301 gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg
     6361 ccctagcgcc cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc
     6421 cccgtcaagc tctaaatcgg gggctccctt tagggttccg atttagtgct ttacggcacc
     6481 tcgaccccaa aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga
     6541 cggtttttcg ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa
     6601 ctggaacaac actcaaccct atctcggtct attcttttga tttataaggg attttgccga
     6661 tttcggccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattaattct
     6721 gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
     6781 gcaaagcatg catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc
     6841 aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac
     6901 tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact
     6961 aatttttttt atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta
     7021 gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc
     7081 cattttcgga tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata
     7141 gtataatacg acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg
     7201 ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc
     7261 cgggacttcg tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc
     7321 agcgcggtcc aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc
     7381 ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc
     7441 gggccggcca tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac
     7501 ccggccggca actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat
     7561 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
     7621 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
     7681 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
     7741 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
     7801 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
     7861 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7921 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7981 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     8041 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     8101 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     8161 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     8221 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     8281 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     8341 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8401 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     8461 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8521 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8581 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8641 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     8701 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8761 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8821 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8881 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8941 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     9001 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     9061 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     9121 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     9181 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     9241 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     9301 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9361 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     9421 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9481 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9541 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9601 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9661 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9721 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9781 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9841 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9901 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9961 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc
    10021 ccgatcccct atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt
    10081 atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta
    10141 caacaaggca aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg
    10201 cgctgcttcg cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt
    10261 aatagtaatc aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat
    10321 aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa
    10381 taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacg
//
