LOCUS       sequence-424312-        6054 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GABA Sensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6054)
  AUTHORS   Marvin JS, Shimoda Y, Magloire V, Leite M, Kawashima T, Jensen TP, 
            Kolb I, Knott EL, Novak O, Podgorski K, Leidenheimer NJ, Rusakov DA,
            Ahrens MB, Kullmann DM, Looger LL
  TITLE     A genetically encoded fluorescent sensor for in vivo imaging of 
            GABA.
  JOURNAL   Nat Methods. 2019 Aug;16(8):763-770. doi: 10.1038/s41592-019-0471-2.
            Epub 2019 Jul 15.
  PUBMED    31308547
REFERENCE   2  (bases 1 to 6054)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41592-019-0471-2"; journalName: "Nat Methods"; date: 
            "2019-08"; volume: "16"; issue: "8"; pages: "763-770"
COMMENT     Sequence Label: pAAV.hSynap.iGABASnFR
FEATURES             Location/Qualifiers
     source          1..6054
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   4..133
                     /label=AAV2 ITR
     promoter        209..656
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     regulatory      685..694
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     sig_peptide     691..750
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
     primer_bind     1797..1818
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(1921..1942)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     CDS             2449..2478
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             2482..2628
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     misc_feature    2644..3232
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2697..2717)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(3115..3126)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(3235..3251)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3236..3252)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     polyA_signal    3281..3402
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3292..3311)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3346..3365
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3781..4369)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3861..3880)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4540..5331,5332..5400))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5163..5182
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5401..5505)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5531..5986)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5668..5689)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5880..5899
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 cagctgcgcg ctcgctcgct cactgaggcc gcccgggcaa agcccgggcg tcgggcgacc
       61 tttggtcgcc cggcctcagt gagcgagcga gcgcgcagag agggagtggc caactccatc
      121 actaggggtt ccttgtagtt aatgattaac ccgccatgct acttatctac gtagccatgc
      181 tctaggaaga tccagggccc tgcgtatgag tgcaagtggg ttttaggacc aggatgaggc
      241 ggggtggggg tgcctacctg acgaccggcc ccgacccact ggacaagcac ccaaccccca
      301 ttccccaaat tgcgcatccc ctatcagaga gggggagggg aaacaggatg cggcgaggcg
      361 cgtgcgcact gccagcttca gcaccgcgga cagtgccttc gcccccgcct ggcggcgcgc
      421 gccaccgccg cctcagcact gaaggcgcgc tgacgtcact cgccggtccc ccgcaaactc
      481 cccttcccgg ccaccttggt cgcgtccgcg ccgccgccgg cccagccgga ccgcaccacg
      541 cgaggcgcga gatagggggg cacgggcgcg accatctgcg ctgcggcgcc ggcgactcag
      601 cgctgcctca gtctgcggtg ggcagcggag gagtcgtgtc gtgcctgaga gcgcagtcca
      661 attcaagctg ctagcaagga tcccgccacc atggagacag acacactcct gctatgggta
      721 ctgctgctct gggttccagg ttccactggt gacagatctg aaagcatcaa tttcgtgagc
      781 tggggcggta gcacccagga tgcgcagaag caggcctggg ccgacccgtt cagcaaggcc
      841 agcggcatta ccgtggtcca ggatgggccc accgactacg gcaaactcaa ggccatggtc
      901 gaaagcggca acgtgcagtg ggacgtggtg gatgtagagg ccgacttcgc cttgcgcgcc
      961 gccgccgaag gcctgctcga acccctggat ttctcggtga tccagcgcga caagatcgac
     1021 ccgcgcttcg tctccgacca tggcgtgggc tcgttcctgt tctctttcgt gctcggctac
     1081 aacgaaggca agctcggagc cagcaagccc caggactgga ccgcgctgtt cgacaccaag
     1141 acctaccccg gcaaacgcgc cctctacaaa tggccgagcc ctggcgtgct cgaactggcc
     1201 ctgctcgccg acggcgtacc ggccgacaag ctctacccgc tggacctgga ccgcgccttc
     1261 aagaaactcg acaccatcaa gaaagacatc gtctggtggg gcggcggtgc acagtcgcag
     1321 cagctgctgg cctccggcga agtcagcatg ggccagttct ggaacggtcg catccacgcc
     1381 ctgcaagaag acggcgcgcc agtgggcgtg agctggaagc agaacctggt gatggccgac
     1441 atcctcgtcg tgcctaaagg cacgaagaac aaagccgccg cgatgaagtt cctggccagt
     1501 gccagcagcg ccaaaggcca ggacgacttt tccgccctga ccgcctatgc tccagtgaac
     1561 atcgacagtg tgcagcgcct cgaccttgcg caggtccgga tcaccgccga caagcagaag
     1621 aacggcatca tggcgaactt caagatccgc cacaacgtgg aggacggcag cgtgcagctc
     1681 gccgaccact accagcagaa cacccccatc ggcgacggcc ccgtgctgct gcccgacaac
     1741 cactacctga gcacccagtc cgtgctgagc aaagacccca acgagaagcg cgatcacatg
     1801 gtcctgctgg agttcgtgac cgccgccggg atcactctcg gcatggacga gctgtacaag
     1861 ggcggtaccg gagggagcat gagcaagggc gaggagctgt tcaccggggt ggtgcccatc
     1921 ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgcgcgg cgagggcgag
     1981 ggcgatgcca ccaacggcaa gctgaccctg aagttcatct gcaccaccgg caagctgccc
     2041 gtgccctggc ccaccctcgt gaccaccctg acctacggcg tgcagtgctt cagccgctac
     2101 cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg ctacgtccag
     2161 gagcgcacca tcagcttcaa ggacgacggc acctacaaga cccgcgccga ggtgaagttc
     2221 gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc
     2281 aacatcctgg ggcacaagct ggagtacaac tggaacgcca acctggcacc gaacctgccg
     2341 accgcctacg tcaaggatca gatcaccctc gatttcgcct actgggccaa gaacggtccg
     2401 gccattgcga cacggtggaa tgaatggcta gtcaaactgc aggtcgacga acaaaaactc
     2461 atctcagaag aggatctgaa tgctgtgggc caggacacgc aggaggtcat cgtggtgcca
     2521 cactccttgc cctttaaggt ggtggtgatc tcagccatcc tggccctggt ggtgctcacc
     2581 atcatctccc ttatcatcct catcatgctt tggcagaaga agccacgtta gaagcttatc
     2641 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     2701 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     2761 cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct ttatgaggag
     2821 ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc
     2881 actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc tttccccctc
     2941 cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac aggggctcgg
     3001 ctgttgggca ctgacaattc cgtggtgttg tcggggaaat catcgtcctt tccttggctg
     3061 ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt cccttcggcc
     3121 ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt
     3181 cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc gcatcgatac
     3241 cgtcgacctc gacccgggcg gccgcttcga gcagacatga taagatacat tgatgagttt
     3301 ggacaaacca caactagaat gcagtgaaaa aaatgcttta tttgtgaaat ttgtgatgct
     3361 attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa caattgcatt
     3421 cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagcaa gtaaaacctc
     3481 tacaaatgtg gtaaaatcga taaggatctt cctagagcat ggctacgtag ataagtagca
     3541 tggcgggtta atcattaact acaaggaacc cctagtgatg gagttggcca ctccctctct
     3601 gcgcgctcgc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc
     3661 agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa
     3721 catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt
     3781 tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg
     3841 gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg
     3901 ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag
     3961 cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc
     4021 caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa
     4081 ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg
     4141 taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc
     4201 taactacggc tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac
     4261 cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg
     4321 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     4381 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     4441 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     4501 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     4561 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     4621 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     4681 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     4741 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     4801 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     4861 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     4921 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     4981 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5041 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5101 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5161 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5221 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     5281 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     5341 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     5401 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     5461 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     5521 agtgccacct aaattgtaag cgttaatatt ttgttaaaat tcgcgttaaa tttttgttaa
     5581 atcagctcat tttttaacca ataggccgaa atcggcaaaa tcccttataa atcaaaagaa
     5641 tagaccgaga tagggttgag tgttgttcca gtttggaaca agagtccact attaaagaac
     5701 gtggactcca acgtcaaagg gcgaaaaacc gtctatcagg gcgatggccc actacgtgaa
     5761 ccatcaccct aatcaagttt tttggggtcg aggtgccgta aagcactaaa tcggaaccct
     5821 aaagggagcc cccgatttag agcttgacgg ggaaagccgg cgaacgtggc gagaaaggaa
     5881 gggaagaaag cgaaaggagc gggcgctagg gcgctggcaa gtgtagcggt cacgctgcgc
     5941 gtaaccacca cacccgccgc gcttaatgcg ccgctacagg gcgcgtccca ttcgccattc
     6001 aggctgcgca actgttggga agggcgatcg gtgcgggcct cttcgctatt acgc
//
