LOCUS       sequence-424313-        4212 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  T7 inducible LanTERN (pRSET).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Jones EM, Su Y, Sander C, Justman QA, Springer M, Silver PA
  TITLE     LanTERN: A Fluorescent Sensor That Specifically Responds to 
            Lanthanides.
  JOURNAL   ACS Synth Biol. 2024 Feb 20. doi: 10.1021/acssynbio.3c00600.
  PUBMED    38377571
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2024 Feb 20. doi: 10.1021/acssynbio.3c00600."
COMMENT     Sequence Label: pEMJ_042
FEATURES             Location/Qualifiers
     source          1..4212
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     220..237
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     322..341
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        322..340
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     RBS             372..394
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             414..431
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             435..467
                     /codon_start=1
                     /product="leader peptide from bacteriophage T7 gene 10"
                     /label=T7 tag (gene 10 leader)
                     /note="promotes efficient translation in E. coli"
                     /translation="MASMTGGQQMG"
     CDS             join(471..479,480..494)
                     /codon_start=1
                     /product="Xpress(TM) epitope tag, including an enterokinase
                     recognition and cleavage site"
                     /label=Xpress(TM) tag
                     /translation="DLYDDDDK"
     regulatory      501..510
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             522..578
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     complement(1915..1933)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      1929..1976
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
     rep_origin      2073..2528
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2160..2179)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     2370..2391
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        2554..2658
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2659..2727,2728..3519)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2877..2896)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(3690..4212,1..66)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     4179..4198
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
       61 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
      121 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
      181 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
      241 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
      301 aggatctcga tcccgcgaaa ttaatacgac tcactatagg gagaccacaa cggtttccct
      361 ctagaaataa ttttgtttaa ctttaagaag gagatataca tatgcggggt tctcatcatc
      421 atcatcatca tggtatggct agcatgactg gtggacagca aatgggtcgg gatctgtacg
      481 acgatgacga taaggatctc gccaccatgg tcgactcatc acgtcgtaag tggaataaga
      541 caggtcacgc agtcagagct ataggtcggc tgagctcact cgagaacgtg tatattaccg
      601 cggataaaca gaaaaacggc attaaagcga actttcacgt gcgccataac gtggaagatg
      661 gcagcgtgca gctggcggat cattatcagc agaacacccc gattggcgat ggcccggtgc
      721 tgctgccgga taaccattat ctgagcaccc agaccaagct gagcaaagat ccgaacgaaa
      781 aacgcgatca catggtgctg ctggaatttg tgaccgcagc gggcattaca cacggcatgg
      841 atgaactgta tggcggcacc ggcggcagcg cgagccaggg cgaagaactg tttaccggcg
      901 tggtgccgat tctggtggaa ctggatggcg atgtgaacgg ccataaattt agcgtgcgcg
      961 gcgaaggcga aggcgatgcg accattggca aactgaccct gaaatttatt tccaccaccg
     1021 gcaaactacc ggtgccgtgg ccgaccctgg tgaccacctt aacctatggc gtgcagtgct
     1081 ttagccgcta tccggatcat atgaaacgcc atgatttttt taaaagcgcg atgccggaag
     1141 gctatgtgca ggaacgcacc attagcttta aagatgatgg caaatataaa acccgcgcgg
     1201 tggtgaaatt tgaaggcgat accctggtga accgcattga actgaaaggc accgatttta
     1261 aagaagatgg caacattctg gggcataaac tggaatataa ccttcctgac caactgactg
     1321 aagagcagat cgcagaattt aaagaggctt tctccctatt tgacccggac ggggatggga
     1381 caatagatac caaggagctg gggacggtga tgcggtctct ggggcagaac cccacagaag
     1441 cagagctgca ggacatgatc aatgaagtag atccggacgg tgatggcaca atcgacttcc
     1501 ctgagttcct gacaatgatg gcaagaaaaa tgaaagacac ggactcggaa gaagaaatta
     1561 gagaagcgtt cagggtgttt gatccggatg gcgatggcta catcgacgca gcagagcttc
     1621 gccacgtgat gacaaacctt ggagagaagt taacagatga agaggttgat gaaatgatca
     1681 gggaagcaga cccggatggg gatggtcagg tagattacga agagtttgta caaatgatga
     1741 cagcgaagta agcgactcta gatcataatc agccatacca catttgtaga ggttttactt
     1801 gctttaaaaa acctcccaca cctccccctg aacctgaaac ataaaatgaa tgcaattcga
     1861 agcttgatcc ggctgctaac aaagcccgaa aggaagctga gttggctgct gccaccgctg
     1921 agcaataact agcataaccc cttggggcct ctaaacgggt cttgaggggt tttttgctga
     1981 aaggaggaac tatatccgga tctggcgtaa tagcgaagag gcccgcaccg atcgcccttc
     2041 ccaacagttg cgcagcctga atggcgaatg ggacgcgccc tgtagcggcg cattaagcgc
     2101 ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc
     2161 tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct
     2221 aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa
     2281 acttgattag ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc
     2341 tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact
     2401 caaccctatc tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg
     2461 gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgct
     2521 tacaatttag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc
     2581 taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa
     2641 tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt
     2701 gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct
     2761 gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc
     2821 cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta
     2881 tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac
     2941 tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc
     3001 atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac
     3061 ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg
     3121 gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac
     3181 gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc
     3241 gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt
     3301 gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga
     3361 gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg taagccctcc
     3421 cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag
     3481 atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca
     3541 tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc
     3601 ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca
     3661 gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc
     3721 tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta
     3781 ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt
     3841 ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     3901 gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     3961 ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     4021 tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     4081 ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     4141 agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     4201 agtcctgtcg gg
//
