LOCUS       sequence-424315-       11431 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses mouse AXIN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11431)
  AUTHORS   Wu YQ, Zhang CS, Xiong J, Cai DQ, Wang CZ, Wang Y, Liu YH, Wang Y, 
            Li Y, Wu J, Wu J, Lan B, Wang X, Chen S, Cao X, Wei X, Hu HH, Guo H,
            Yu Y, Ghafoor A, Xie C, Wu Y, Xu Z, Zhang C, Zhu M, Huang X, Sun X, 
            Lin SY, Piao HL, Zhou J, Lin SC
  TITLE     Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine
            synthesis to p53 activation to control cell fate.
  JOURNAL   Cell Res. 2023 Nov;33(11):835-850. doi: 10.1038/s41422-023-00874-4. 
            Epub 2023 Sep 19.
  PUBMED    37726403
REFERENCE   2  (bases 1 to 11431)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41422-023-00874-4"; journalName: "Cell Res"; date: 
            "2023-11"; volume: "33"; issue: "11"; pages: "835-850"
COMMENT     Sequence Label: pBOBI N-HA AXIN1
FEATURES             Location/Qualifiers
     source          1..11431
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        189..387
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             405..585
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    632..757
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1250..1483
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1668..1712
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1964..2080
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2103..2406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2407..2610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2607..2631
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2709..2735
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2742..2768
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2772..2798
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          5405..5634
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5617..5636
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5811..6399
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5864..5884)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6282..6293)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6806..6986
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(7012..7029)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    7018..7242
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7288..7716
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7375..7394)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7585..7606
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7725..7745)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7730..8059
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7910..8045
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7972..7991
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8107..8154
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8173..8547
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8677..8798
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8714..8733)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8768..8787
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8847..8863)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8847..8863)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8860..8882)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8871..8887
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8895..8901,8902..8919,8920..8925))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8940..8961
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9078..9095)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9249..9837)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9329..9348)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(10008..10799,10800..10868))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10631..10650
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10869..10973)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(11048..11067)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(11239..11431,1..187)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
       61 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      121 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      181 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      241 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      301 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      361 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      421 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      481 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      541 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      601 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      661 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      721 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      781 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      841 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      901 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      961 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1021 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1081 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1141 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1201 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1261 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1321 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1381 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1441 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1501 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1561 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1621 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1681 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1741 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1801 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1861 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1921 agagagacag agacagatcc attcgattag tgaacggatc aacttttaaa agaaaagggg
     1981 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2041 ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgataagc ttgggagttc
     2101 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2161 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2221 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2281 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2341 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2401 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2461 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2521 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2581 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     2641 cgccatccac gctgttttga cctccataga agacaccgac tgagctcctt aaggttaacg
     2701 ccaccatgta cccttatgac gtacctgact atgctggcgc ctacccttat gacgtacctg
     2761 actatgctgg atacccttat gacgtacctg actatgctgg atctagagaa ttcggatcca
     2821 tgcagagtcc caaaatgaat gtccaggagc agggtttccc cttggacctc ggagcaagtt
     2881 tcaccgaaga tgccccccgg cccccagtgc ctggagaaga gggagaactg gtatctactg
     2941 attcgaggcc tgtcaaccac agtttctgtt ctgggaaagg taccagcatt aaaagtgaga
     3001 cctcaacagc caccccaaga cgttcagatc tggatctggg atatgagccc gagggcagtg
     3061 cctcccccac cccaccatat ttgaggtggg ctgagtcact gcattcctta ctggatgacc
     3121 aagatgggat cagcctgttc aggactttcc tgaagcagga gggctgtgct gacctgctgg
     3181 acttctggtt tgcctgcagt ggcttcagga agcttgagcc ctgtgactca aatgaggaaa
     3241 agaggctgaa gctggcaaga gccatctacc gaaagtacat cctggatagc aatggcattg
     3301 tgtccagaca aaccaagcca gccactaaga gcttcataaa ggactgtgtc atgaagcagc
     3361 agatagatcc tgccatgttt gaccaggcac agacagaaat ccagtccacc atggaggaga
     3421 atacctaccc ttcctttctt aagtctgaca tttatttgga gtacacaagg acaggctcag
     3481 agagtccgaa ggtctgcagt gaccagagct cagggtctgg aacagggaag ggcatgtctg
     3541 gatacctgcc cactttgaat gaggatgaag aatggaaatg tgaccaagat gcagatgagg
     3601 atgatggccg agaccctctc ccccccagca ggctcaccca gaagctgcta ttggagactg
     3661 ctgccccgag ggccccctca agtagacggt acaacgaagg cagagagctc aggtatggat
     3721 cttggaggga gcccgtcaac ccctactacg tcaactctgg ctatgccctg gccccagcca
     3781 ccagtgccaa tgacagtgag cagcagagcc tgtccagtga tgctgacacg ctatccctta
     3841 cggacagtag tgtggatgga atccccccat acaggatccg taagcagcac cgaagggaga
     3901 tgcaggagag tatccaagtc aatgggcggg tacctctacc tcacattcct cgcacttacc
     3961 gaatgccaaa ggagatccgg gtagagccac agaaatttgc tgaagagctt attcaccgtc
     4021 tagaggctgt ccagcgcact cgagaggctg aagaaaagtt ggaggaacgg ctgaagcgtg
     4081 tacgcatgga ggaagaaggg gaggatggtg aaatgccttc tggccccatg gcaagtcaca
     4141 agctgccttc tgtcccagct tggcaccatt tcccaccccg ctatgtggat atgggctgct
     4201 ctggactgcg ggatgcccat gaggagaatc ctgagagcat cctggatgag cacgtgcaaa
     4261 gggtcatgag gacacctggc tgccagtcac ctggcccagg ccaccgctct cctgacagtg
     4321 ggcatgtggc taagactgca gtgctagggg gtacagcctc cgggcatggg aagcatgttc
     4381 ctaagttagg gttgaagctg gatacagctg gcctgcacca tcatagacat gtccaccacc
     4441 atgttcacca taattcagct agacctaagg agcaaatgga ggctgaagtt gcccgcaggg
     4501 tccagagcag cttctcgtgg ggcccagaaa cacatggtca tgccaagccc cggagctatt
     4561 ccgagaacgc aggcaccacc ctcagtgctg gggatttggc ctttggtggt aaaactagtg
     4621 caccttccaa aagaaacacc aagaaggctg aatctgggaa gaatgccaat gctgaggtac
     4681 ccagtaccac agaggacgct gagaagaacc agaagatcat gcagtggatc attgagggag
     4741 agaaggagat cagtagacac cggaaggcag gccatgggtc ttctgggttg aggaagcagc
     4801 aggcccatga aagctccagg cccttgtcca tcgagcgtcc tggggccgtg cacccctggg
     4861 tcagcgctca gcttcggaat tctgtccagc cttctcatct tttcatccaa gatcccacaa
     4921 tgccacccaa tccagcccct aatcccctga cccagctgga agaggcccgc aggcgtttgg
     4981 aagaagaaga aaagagagca aacaaactgc cctccaagca gaggacaaaa tcacaaagaa
     5041 aggcaggtgg cgggagtgca ccaccatgtg acagcattgt tgtggcctac tatttctgtg
     5101 gggaacccat cccctaccgg accctggtga ggggccgtgc tgtcaccctg ggccagttca
     5161 aggagctgct aaccaagaag gggagctaca gatactactt taagaaagtg agtgatgagt
     5221 ttgactgtgg tgtggtattt gaggaagtac gggaggatga ggccgtcttg cctgtctttg
     5281 aagaaaagat catcggcaag gtggaaaagg tggactgact cgagccatgg aagcttgata
     5341 tctaactgac tgaaccggtg gtaccgagga attaattcgc tgtctgcgag ggccagctgt
     5401 tggggtgagt actccctctc aaaagcgggc atgacttctg cgctaagatt gtcagtttcc
     5461 aaaaacgagg aggatttgat attcacctgg cccgcggtga tgcctttgag ggtggccgcg
     5521 tccatctggt cagaaaagac aatctttttg ttgtcaagct tgaggtgtgg caggcttgag
     5581 atctggccat acacttgagt gacaatgaca tccactttgc ctttctctcc acaggtgtcc
     5641 actcccaggt ccaactgcag gtcgagcatg catctagggc ggccaattcc gccgatcctg
     5701 gaaaaacatg gagcaatcac aagtagcaat acagcagcta ccaatgctga ttgtgcctgg
     5761 ctagaagcac aagaggagga ggaggtgggt tttccagtca cacctcagac aatcaacctc
     5821 tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct ccttttacgc
     5881 tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt atggctttca
     5941 ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg tggcccgttg
     6001 tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact ggttggggca
     6061 ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct attgccacgg
     6121 cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg ttgggcactg
     6181 acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc gcctgtgttg
     6241 ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc aatccagcgg
     6301 accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt cgccttcgcc
     6361 ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga gctcggtacc
     6421 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
     6481 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
     6541 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
     6601 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
     6661 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
     6721 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
     6781 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
     6841 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
     6901 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
     6961 ttagtcagtg tggaaaatct ctagcagggc cgctttaaac ccgctgatca gcctcgactg
     7021 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
     7081 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
     7141 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
     7201 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
     7261 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
     7321 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
     7381 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
     7441 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
     7501 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
     7561 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
     7621 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
     7681 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
     7741 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
     7801 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
     7861 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
     7921 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
     7981 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
     8041 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
     8101 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
     8161 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
     8221 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
     8281 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
     8341 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
     8401 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
     8461 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
     8521 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
     8581 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
     8641 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
     8701 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8761 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
     8821 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     8881 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     8941 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     9001 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
     9061 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
     9121 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
     9181 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
     9241 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
     9301 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
     9361 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
     9421 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
     9481 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
     9541 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
     9601 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
     9661 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
     9721 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
     9781 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
     9841 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
     9901 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
     9961 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    10021 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    10081 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    10141 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    10201 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    10261 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    10321 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    10381 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    10441 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    10501 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    10561 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    10621 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    10681 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    10741 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    10801 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    10861 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    10921 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    10981 ccccgaaaag tgccacctga cgtcgacgga tcgggagatc tcccgatccc ctatggtgca
    11041 ctctcagtac aatctgctct gatgccgcat agttaagcca gtatctgctc cctgcttgtg
    11101 tgttggaggt cgctgagtag tgcgcgagca aaatttaagc tacaacaagg caaggcttga
    11161 ccgacaattg catgaagaat ctgcttaggg ttaggcgttt tgcgctgctt cgcgatgtac
    11221 gggccagata tacgcgttga cattgattat tgactagtta ttaatagtaa tcaattacgg
    11281 ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg gtaaatggcc
    11341 cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg tatgttccca
    11401 tagtaacgcc aatagggact ttccattgac g
//
