LOCUS       sequence-424319-       12429 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses mouse HIPK2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12429)
  AUTHORS   Wu YQ, Zhang CS, Xiong J, Cai DQ, Wang CZ, Wang Y, Liu YH, Wang Y, 
            Li Y, Wu J, Wu J, Lan B, Wang X, Chen S, Cao X, Wei X, Hu HH, Guo H,
            Yu Y, Ghafoor A, Xie C, Wu Y, Xu Z, Zhang C, Zhu M, Huang X, Sun X, 
            Lin SY, Piao HL, Zhou J, Lin SC
  TITLE     Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine
            synthesis to p53 activation to control cell fate.
  JOURNAL   Cell Res. 2023 Nov;33(11):835-850. doi: 10.1038/s41422-023-00874-4. 
            Epub 2023 Sep 19.
  PUBMED    37726403
REFERENCE   2  (bases 1 to 12429)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41422-023-00874-4"; journalName: "Cell Res"; date: 
            "2023-11"; volume: "33"; issue: "11"; pages: "835-850"
COMMENT     Sequence Label: pBOBI N-FLAG HIPK2
FEATURES             Location/Qualifiers
     source          1..12429
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        189..387
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             405..585
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    632..757
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1250..1483
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1668..1712
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1964..2080
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2103..2406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2407..2610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2607..2631
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     regulatory      2699..2708
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             2708..2731
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     intron          6403..6632
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     6615..6634
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    6809..7397
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(6862..6882)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7280..7291)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             7804..7984
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8010..8027)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8016..8240
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8286..8714
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8373..8392)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8583..8604
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(8723..8743)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8728..9057
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8908..9043
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8970..8989
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9105..9152
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9171..9545
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    9675..9796
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9712..9731)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9766..9785
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9845..9861)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(9845..9861)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(9858..9880)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    9869..9885
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(9893..9899,9900..9917,9918..9923))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    9938..9959
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10076..10093)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10247..10835)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10327..10346)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11006..11797,11798..11866))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     11629..11648
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(11867..11971)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(12046..12065)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(12237..12429,1..187)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
       61 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      121 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      181 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      241 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      301 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      361 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      421 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      481 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      541 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      601 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      661 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      721 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      781 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      841 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      901 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      961 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1021 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1081 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1141 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1201 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1261 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1321 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1381 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1441 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1501 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1561 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1621 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1681 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1741 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1801 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1861 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1921 agagagacag agacagatcc attcgattag tgaacggatc aacttttaaa agaaaagggg
     1981 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2041 ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgataagc ttgggagttc
     2101 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2161 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2221 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2281 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2341 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2401 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2461 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2521 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2581 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     2641 cgccatccac gctgttttga cctccataga agacaccgac tgagctccta aggttaacgc
     2701 caccatggac tacaaagacg atgacgacaa gtctagagaa ttcggatcca tggcctcaca
     2761 tgtgcaagtt ttctcccctc acacccttca atcaagtgcc ttctgtagtg tgaagaaact
     2821 aaaagtagag ccaagttcca actgggacat gactgggtac ggctcccaca gcaaagtgta
     2881 cagccagagc aagaacatac caccttctca gccagcctcc acaaccgtca gcacctcctt
     2941 gccaatcccc aacccaagcc taccttacga gcagaccatc atcttcccag gaagtactgg
     3001 acacatagtt gtaacatcag caagtagcac ttctgtcacc gggcaagtcc tcggtggacc
     3061 acataaccta atgcgtcgaa gcactgtgag cctccttgac acctaccaaa aatgtggact
     3121 caagcgcaag agtgaggaga ttgagaacac aagcagcgtg cagatcattg aagagcatcc
     3181 acccatgatt cagaataatg ccagcggggc cactgtagcc actgccacca catccactgc
     3241 cacctccaaa aacagtggct ccaacagtga aggggactat cagctggtgc agcatgaagt
     3301 actgtgctcc atgaccaaca cctatgaggt tttagagttc ttggggcgag ggacatttgg
     3361 gcaagtggtc aagtgctgga aacggggcac caatgaaatt gtggccatta agatcctcaa
     3421 gaaccacccc tcctatgccc ggcaaggcca gattgaagtg agcatcttgg cccggctgag
     3481 cacggagagc gctgatgact acaattttgt gcgggcctat gagtgcttcc agcacaagaa
     3541 ccacacgtgc ttagtctttg agatgttgga gcagaacctt tatgactttc tgaaacagaa
     3601 caagtttagt cccttgcccc tcaagtatat tcgcccagtc ctccagcagg tagccacagc
     3661 cctgatgaaa ctcaaaagcc tgggtcttat ccatgctgac ctcaaaccag aaaacatcat
     3721 gctggtggat ccatccagac aaccgtaccg agtgaaggtc attgactttg gttcagctag
     3781 tcatgtgtcc aaagctgtct gctctacgta cttgcaatcc agatactacc gggcccctga
     3841 gatcatcctt ggtttgccat tctgtgaggc aattgacatg tggtcccttg gttgtgtcat
     3901 tgctgaactg ttcctgggct ggccattata cccaggtgct tctgagtatg atcagattcg
     3961 gtatatttca caaacacagg gtttaccagc tgaatattta ttaagtgcag ggacaaagac
     4021 aactaggttt ttcaaccgtg acacagactc accgtatcct ttgtggaggc taaagacacc
     4081 agatgaccat gaagcagaga cgggaattaa gtcaaaggaa gcaagaaagt acattttcaa
     4141 ctgtttggat gatatggccc aggtgaacat gacaacagat ttggaaggga gtgacatgtt
     4201 agtagagaag gcagaccgac gggagttcat tgacctgtta aagaagatgc tgaccatcga
     4261 tgctgataag agagtcactc ccattgaaac tctgaaccac ccctttgtca ccatgacaca
     4321 cctgcttgac ttcccccata gtgcccatgt caaatcttgt ttccaaaaca tggagatttg
     4381 caagcgccgg gtgaatatgt atgacacagt gaaccagagc aaaacacctt tcatcactca
     4441 cgtggccccc agcacatcca ccaacttgac catgaccttt aacaaccagc tgaccactgt
     4501 ccacaaccag gctcccacca cctccagtgc cactctttcc ttagccaatc ccgaagtctc
     4561 catactaaac taccaatctg cactctacca gccctcagcg gcatccatgg ccgcggtggc
     4621 cccgcggagc atgcccctgc agacgggaac agcccaaatt tgtgcccgac ctgatccctt
     4681 ccagcaagct ctcatcgtgt gtccccctgg cttccaaggc ctgcaggcct ctccctccaa
     4741 gcacgctggc tactcagtgc ggatggaaaa tgctgtcccc atcgtcaccc aggcgccagg
     4801 agctcagcct cttcagatcc aaccaggcct gcttgctcag caggcctggc caggtggggc
     4861 ccaacagatc ctacttcctc cagcatggca gcagctgact ggcgtggcca cccacacatc
     4921 tgtacagcat gcagctgtga ttcctgagac catggcaggc actcagcaac tggctgactg
     4981 gaggaacacg catgctcatg gaagtcatta caatcccatc atgcagcagc ctgcactctt
     5041 gactggtcat gtgacccttc cagctgccca gcccttaaat gtgggtgtag cccatgtgat
     5101 gaggcaacaa ccaaccagca ccacctcctc caggaagagt aagcagcacc agtcatctgt
     5161 gagaaatgtc tccacctgtg aggtgacctc ttcacaggct atcagctccc ctcagcgatc
     5221 caagcgtgtc aaggagaaca ctcccccacg gtgcgccatg gtacacagca gcccagcttg
     5281 cagcacctca gtcacctgtg ggtggggcga cgtggcctcc agcaccaccc gggagcgaca
     5341 gcggcagacg attgtcatcc ccgacacccc gagccccaca gtcagtgtca tcaccatcag
     5401 cagtgacacc gatgaagaag aggagcagaa gcacgccccc accagcacag tctccaagca
     5461 aagaaaaaat gtcatcagct gtgtcaccgt ccacgactct ccctactctg actcctccag
     5521 caacaccagc ccctactcgg tgcagcagcg cacagggcac aacggcacca acaccttgga
     5581 caccaagggg ggcctagaga atcactgcac tggcaacccc cgcaccatca tcgtaccccc
     5641 actgaagacc caggccagtg aagtgttggt agaatgtgac agcctagggc cagcgatcag
     5701 tgccagtcac cattcgtcct ccttcaagtc caagtcctcc agcaccgtga cctccaccag
     5761 tggacactct tcagggagct cttcaggagc catcgcctac cgtcagcagc ggccaggccc
     5821 ccacttccag cagcagcagc ccctcaatct cagccaggcc cagcagcaca tggctgcgga
     5881 ccgcaccggg agtcaccgtc gacagcaggc ctacatcact ccgaccatgg cgcaagctcc
     5941 gtacaccttc ccgcacaaca gccccagcca tggcactgtt cacccccacc tggctgcggc
     6001 tgcccacctc cccacccagc ctcacctcta cacctataca gcgcccacag ccctaggctc
     6061 caccggcact gtagcccacc tggtggcatc ccaaggctca gcacgccaca ccgtgcagca
     6121 cactgcctat ccggccagca tcgtccacca ggtccctgtg agcatggggc cccgggtcct
     6181 gccctcgccc accatccacc ccagtcagta tccagcccag tttgcccacc agacctatat
     6241 cagcgcctcg ccagcctcca ccgtctacac tggataccca ctgagtcctg ccaaggtcaa
     6301 ccaataccct tacatactcg agccatggaa gcttgatatc taactgactg aaccggtggt
     6361 accgaggaat taattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa
     6421 aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat
     6481 tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa
     6541 tctttttgtt gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga
     6601 caatgacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcaggt
     6661 cgagcatgca tctagggcgg ccaattccgc cgatcctgga aaaacatgga gcaatcacaa
     6721 gtagcaatac agcagctacc aatgctgatt gtgcctggct agaagcacaa gaggaggagg
     6781 aggtgggttt tccagtcaca cctcagacaa tcaacctctg gattacaaaa tttgtgaaag
     6841 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
     6901 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
     6961 ctggttgctg tctctttatg aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg
     7021 cactgtgttt gctgacgcaa cccccactgg ttggggcatt gccaccacct gtcagctcct
     7081 ttccgggact ttcgctttcc ccctccctat tgccacggcg gaactcatcg ccgcctgcct
     7141 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgttgtcggg
     7201 gaagctgacg tcctttccat ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac
     7261 gtccttctgc tacgtccctt cggccctcaa tccagcggac cttccttccc gcggcctgct
     7321 gccggctctg cggcctcttc cgcgtcttcg ccttcgccct cagacgagtc ggatctccct
     7381 ttgggccgcc tccccgcctg gaattcgagc tcggtacctt taagaccaat gacttacaag
     7441 gcagctgtag atcttagcca ctttttaaaa gaaaaggggg gactggaagg gctaattcac
     7501 tcccaacgaa gacaagatat ccttgatctg tggatctacc acacacaagg ctacttccct
     7561 gattggcaga actacacacc agggccaggg atcagatatc cactgacctt tggatggtgc
     7621 tacaagctag taccagttga gcaagagaag gtagaagaag ccaatgaagg agagaacacc
     7681 cgcttgttac accctgtgag cctgcatggg atggatgacc cggagagaga agtattagag
     7741 tggaggtttg acagccgcct agcatttcat cacatggccc gagagctgca tccggactgt
     7801 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     7861 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     7921 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     7981 agcagggccg ctttaaaccc gctgatcagc ctcgactgtg ccttctagtt gccagccatc
     8041 tgttgtttgc ccctcccccg tgccttcctt gaccctggaa ggtgccactc ccactgtcct
     8101 ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt aggtgtcatt ctattctggg
     8161 gggtggggtg gggcaggaca gcaaggggga ggattgggaa gacaatagca ggcatgctgg
     8221 ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc agctggggct ctagggggta
     8281 tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     8341 gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     8401 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     8461 atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     8521 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     8581 tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     8641 tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     8701 atttaacgcg aattaattct gtggaatgtg tgtcagttag ggtgtggaaa gtccccaggc
     8761 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac caggtgtgga
     8821 aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     8881 accatagtcc cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat
     8941 tctccgcccc atggctgact aatttttttt atttatgcag aggccgaggc cgcctctgcc
     9001 tctgagctat tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag
     9061 ctcccgggag cttgtatatc cattttcgga tctgatcagc acgtgttgac aattaatcat
     9121 cggcatagta tatcggcata gtataatacg acaaggtgag gaactaaacc atggccaagt
     9181 tgaccagtgc cgttccggtg ctcaccgcgc gcgacgtcgc cggagcggtc gagttctgga
     9241 ccgaccggct cgggttctcc cgggacttcg tggaggacga cttcgccggt gtggtccggg
     9301 acgacgtgac cctgttcatc agcgcggtcc aggaccaggt ggtgccggac aacaccctgg
     9361 cctgggtgtg ggtgcgcggc ctggacgagc tgtacgccga gtggtcggag gtcgtgtcca
     9421 cgaacttccg ggacgcctcc gggccggcca tgaccgagat cggcgagcag ccgtgggggc
     9481 gggagttcgc cctgcgcgac ccggccggca actgcgtgca cttcgtggcc gaggagcagg
     9541 actgacacgt gctacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg
     9601 gaatcgtttt ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt
     9661 tcttcgccca ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca
     9721 tcacaaattt cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac
     9781 tcatcaatgt atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat
     9841 catggtcata gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac
     9901 gagccggaag cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa
     9961 ttgcgttgcg ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat
    10021 gaatcggcca acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc
    10081 tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
    10141 cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
    10201 gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
    10261 gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
    10321 gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
    10381 ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
    10441 atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
    10501 tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
    10561 ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
    10621 gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
    10681 ctagaagaac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
    10741 ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
    10801 agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
    10861 ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
    10921 aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
    10981 tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
    11041 cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
    11101 tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
    11161 cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
    11221 ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
    11281 gttcgccagt taatagtttg cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac
    11341 gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
    11401 gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
    11461 gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
    11521 tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
    11581 aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aatacgggat aataccgcgc
    11641 cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
    11701 caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
    11761 cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
    11821 ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
    11881 aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
    11941 tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
    12001 tcgacggatc gggagatctc ccgatcccct atggtgcact ctcagtacaa tctgctctga
    12061 tgccgcatag ttaagccagt atctgctccc tgcttgtgtg ttggaggtcg ctgagtagtg
    12121 cgcgagcaaa atttaagcta caacaaggca aggcttgacc gacaattgca tgaagaatct
    12181 gcttagggtt aggcgttttg cgctgcttcg cgatgtacgg gccagatata cgcgttgaca
    12241 ttgattattg actagttatt aatagtaatc aattacgggg tcattagttc atagcccata
    12301 tatggagttc cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
    12361 cccccgccca ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt
    12421 ccattgacg
//
