LOCUS       sequence-424321-       11368 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses mouse AXIN1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11368)
  AUTHORS   Wu YQ, Zhang CS, Xiong J, Cai DQ, Wang CZ, Wang Y, Liu YH, Wang Y, 
            Li Y, Wu J, Wu J, Lan B, Wang X, Chen S, Cao X, Wei X, Hu HH, Guo H,
            Yu Y, Ghafoor A, Xie C, Wu Y, Xu Z, Zhang C, Zhu M, Huang X, Sun X, 
            Lin SY, Piao HL, Zhou J, Lin SC
  TITLE     Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine
            synthesis to p53 activation to control cell fate.
  JOURNAL   Cell Res. 2023 Nov;33(11):835-850. doi: 10.1038/s41422-023-00874-4. 
            Epub 2023 Sep 19.
  PUBMED    37726403
REFERENCE   2  (bases 1 to 11368)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41422-023-00874-4"; journalName: "Cell Res"; date: 
            "2023-11"; volume: "33"; issue: "11"; pages: "835-850"
COMMENT     Sequence Label: pBOBI N-HA AXIN1 delta PHGDH (delta 321-340)
FEATURES             Location/Qualifiers
     source          1..11368
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        189..387
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             405..585
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    632..757
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1250..1483
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1668..1712
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1964..2080
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2103..2406
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2407..2610
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2607..2631
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             2709..2735
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2742..2768
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2772..2798
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     intron          5342..5571
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     5554..5573
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    5748..6336
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5801..5821)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(6219..6230)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6743..6923
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(6949..6966)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    6955..7179
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      7225..7653
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(7312..7331)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7522..7543
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(7662..7682)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7667..7996
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      7847..7982
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     7909..7928
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        8044..8091
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             8110..8484
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    8614..8735
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8651..8670)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8705..8724
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8784..8800)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8784..8800)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8797..8819)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8808..8824
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8832..8838,8839..8856,8857..8862))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8877..8898
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(9015..9032)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9186..9774)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9266..9285)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9945..10736,10737..10805))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10568..10587
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10806..10910)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(10985..11004)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        join(11176..11368,1..187)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
       61 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      121 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      181 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
      241 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
      301 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
      361 tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct ctctggttag
      421 accagatctg agcctgggag ctctctggct aactagggaa cccactgctt aagcctcaat
      481 aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac tctggtaact
      541 agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc gcccgaacag
      601 ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc ggcttgctga
      661 agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa ttttgactag
      721 cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg ggagaattag
      781 atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata aattaaaaca
      841 tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc tgttagaaac
      901 atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga caggatcaga
      961 agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc aaaggataga
     1021 gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca aaagtaagac
     1081 caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg agggacaatt
     1141 ggagaagtga attatataaa tataaagtag taaaaattga accattagga gtagcaccca
     1201 ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata ggagctttgt
     1261 tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg acgctgacgg
     1321 tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg ctgagggcta
     1381 ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag ctccaggcaa
     1441 gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt tggggttgct
     1501 ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt aataaatctc
     1561 tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca
     1621 caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag
     1681 aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc
     1741 tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt
     1801 ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga
     1861 cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag
     1921 agagagacag agacagatcc attcgattag tgaacggatc aacttttaaa agaaaagggg
     1981 ggattggggg gtacagtgca ggggaaagaa tagtagacat aatagcaaca gacatacaaa
     2041 ctaaagaatt acaaaaacaa attacaaaat tcaaaatttt atcgataagc ttgggagttc
     2101 cgcgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga cccccgccca
     2161 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     2221 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     2281 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     2341 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     2401 accatggtga tgcggttttg gcagtacatc aatgggcgtg gatagcggtt tgactcacgg
     2461 ggatttccaa gtctccaccc cattgacgtc aatgggagtt tgttttggca ccaaaatcaa
     2521 cgggactttc caaaatgtcg taacaactcc gccccattga cgcaaatggg cggtaggcgt
     2581 gtacggtggg aggtctatat aagcagagct cgtttagtga accgtcagat cgcctggaga
     2641 cgccatccac gctgttttga cctccataga agacaccgac tgagctcctt aaggttaacg
     2701 ccaccatgta cccttatgac gtacctgact atgctggcgc ctacccttat gacgtacctg
     2761 actatgctgg atacccttat gacgtacctg actatgctgg atctagagaa ttcggatcca
     2821 tgcagagtcc caaaatgaat gtccaggagc agggtttccc cttggacctc ggagcaagtt
     2881 tcaccgaaga tgccccccgg cccccagtgc ctggagaaga gggagaactg gtatctactg
     2941 attcgaggcc tgtcaaccac agtttctgtt ctgggaaagg taccagcatt aaaagtgaga
     3001 cctcaacagc caccccaaga cgttcagatc tggatctggg atatgagccc gagggcagtg
     3061 cctcccccac cccaccatat ttgaggtggg ctgagtcact gcattcctta ctggatgacc
     3121 aagatgggat cagcctgttc aggactttcc tgaagcagga gggctgtgct gacctgctgg
     3181 acttctggtt tgcctgcagt ggcttcagga agcttgagcc ctgtgactca aatgaggaaa
     3241 agaggctgaa gctggcaaga gccatctacc gaaagtacat cctggatagc aatggcattg
     3301 tgtccagaca aaccaagcca gccactaaga gcttcataaa ggactgtgtc atgaagcagc
     3361 agatagatcc tgccatgttt gaccaggcac agacagaaat ccagtccacc atggaggaga
     3421 atacctaccc ttcctttctt aagtctgaca tttatttgga gtacacaagg acaggctcag
     3481 agagtccgaa ggtctgcagt gaccagagct cagggtctgg aacagggaag ggcatgtctg
     3541 gatacctgcc cactttgaat gaggatgaag aatggaaatg tgaccaagat gcagatgagg
     3601 atgatggccg agaccctctc ccccccagca ggctcaccca gaagctgcta ttggagactg
     3661 ctgccccgag ggccccctca agtagacggt acaacgaagg cagagagctc aggtatggat
     3721 cttggaggga gcccgtcaac ccctactacg tcaactctgg ctatgccctg gccccagcca
     3781 cggacagtag tgtggatgga atccccccat acaggatccg taagcagcac cgaagggaga
     3841 tgcaggagag tatccaagtc aatgggcggg tacctctacc tcacattcct cgcacttacc
     3901 gaatgccaaa ggagatccgg gtagagccac agaaatttgc tgaagagctt attcaccgtc
     3961 tagaggctgt ccagcgcact cgagaggctg aagaaaagtt ggaggaacgg ctgaagcgtg
     4021 tacgcatgga ggaagaaggg gaggatggtg aaatgccttc tggccccatg gcaagtcaca
     4081 agctgccttc tgtcccagct tggcaccatt tcccaccccg ctatgtggat atgggctgct
     4141 ctggactgcg ggatgcccat gaggagaatc ctgagagcat cctggatgag cacgtgcaaa
     4201 gggtcatgag gacacctggc tgccagtcac ctggcccagg ccaccgctct cctgacagtg
     4261 ggcatgtggc taagactgca gtgctagggg gtacagcctc cgggcatggg aagcatgttc
     4321 ctaagttagg gttgaagctg gatacagctg gcctgcacca tcatagacat gtccaccacc
     4381 atgttcacca taattcagct agacctaagg agcaaatgga ggctgaagtt gcccgcaggg
     4441 tccagagcag cttctcgtgg ggcccagaaa cacatggtca tgccaagccc cggagctatt
     4501 ccgagaacgc aggcaccacc ctcagtgctg gggatttggc ctttggtggt aaaactagtg
     4561 caccttccaa aagaaacacc aagaaggctg aatctgggaa gaatgccaat gctgaggtac
     4621 ccagtaccac agaggacgct gagaagaacc agaagatcat gcagtggatc attgagggag
     4681 agaaggagat cagtagacac cggaaggcag gccatgggtc ttctgggttg aggaagcagc
     4741 aggcccatga aagctccagg cccttgtcca tcgagcgtcc tggggccgtg cacccctggg
     4801 tcagcgctca gcttcggaat tctgtccagc cttctcatct tttcatccaa gatcccacaa
     4861 tgccacccaa tccagcccct aatcccctga cccagctgga agaggcccgc aggcgtttgg
     4921 aagaagaaga aaagagagca aacaaactgc cctccaagca gaggacaaaa tcacaaagaa
     4981 aggcaggtgg cgggagtgca ccaccatgtg acagcattgt tgtggcctac tatttctgtg
     5041 gggaacccat cccctaccgg accctggtga ggggccgtgc tgtcaccctg ggccagttca
     5101 aggagctgct aaccaagaag gggagctaca gatactactt taagaaagtg agtgatgagt
     5161 ttgactgtgg tgtggtattt gaggaagtac gggaggatga ggccgtcttg cctgtctttg
     5221 aagaaaagat catcggcaag gtggaaaagg tggacctcga gccatggaag cttgatatct
     5281 aactgactga accggtggta ccgaggaatt aattcgctgt ctgcgagggc cagctgttgg
     5341 ggtgagtact ccctctcaaa agcgggcatg acttctgcgc taagattgtc agtttccaaa
     5401 aacgaggagg atttgatatt cacctggccc gcggtgatgc ctttgagggt ggccgcgtcc
     5461 atctggtcag aaaagacaat ctttttgttg tcaagcttga ggtgtggcag gcttgagatc
     5521 tggccataca cttgagtgac aatgacatcc actttgcctt tctctccaca ggtgtccact
     5581 cccaggtcca actgcaggtc gagcatgcat ctagggcggc caattccgcc gatcctggaa
     5641 aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg tgcctggcta
     5701 gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcagacaat caacctctgg
     5761 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     5821 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     5881 tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg cccgttgtca
     5941 ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt tggggcattg
     6001 ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt gccacggcgg
     6061 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     6121 attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc tgtgttgcca
     6181 cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat ccagcggacc
     6241 ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc cttcgccctc
     6301 agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattcgagct cggtaccttt
     6361 aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag aaaagggggg
     6421 actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt ggatctacca
     6481 cacacaaggc tacttccctg attggcagaa ctacacacca gggccaggga tcagatatcc
     6541 actgaccttt ggatggtgct acaagctagt accagttgag caagagaagg tagaagaagc
     6601 caatgaagga gagaacaccc gcttgttaca ccctgtgagc ctgcatggga tggatgaccc
     6661 ggagagagaa gtattagagt ggaggtttga cagccgccta gcatttcatc acatggcccg
     6721 agagctgcat ccggactgta ctgggtctct ctggttagac cagatctgag cctgggagct
     6781 ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt gagtgcttca
     6841 agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca gaccctttta
     6901 gtcagtgtgg aaaatctcta gcagggccgc tttaaacccg ctgatcagcc tcgactgtgc
     6961 cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg accctggaag
     7021 gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat tgtctgagta
     7081 ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag gattgggaag
     7141 acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg gaaagaacca
     7201 gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     7261 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc gctcctttcg
     7321 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     7381 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     7441 agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     7501 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaacccta
     7561 tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat tggttaaaaa
     7621 atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt gtcagttagg
     7681 gtgtggaaag tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta
     7741 gtcagcaacc aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     7801 gcatctcaat tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac
     7861 tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga
     7921 ggccgaggcc gcctctgcct ctgagctatt ccagaagtag tgaggaggct tttttggagg
     7981 cctaggcttt tgcaaaaagc tcccgggagc ttgtatatcc attttcggat ctgatcagca
     8041 cgtgttgaca attaatcatc ggcatagtat atcggcatag tataatacga caaggtgagg
     8101 aactaaacca tggccaagtt gaccagtgcc gttccggtgc tcaccgcgcg cgacgtcgcc
     8161 ggagcggtcg agttctggac cgaccggctc gggttctccc gggacttcgt ggaggacgac
     8221 ttcgccggtg tggtccggga cgacgtgacc ctgttcatca gcgcggtcca ggaccaggtg
     8281 gtgccggaca acaccctggc ctgggtgtgg gtgcgcggcc tggacgagct gtacgccgag
     8341 tggtcggagg tcgtgtccac gaacttccgg gacgcctccg ggccggccat gaccgagatc
     8401 ggcgagcagc cgtgggggcg ggagttcgcc ctgcgcgacc cggccggcaa ctgcgtgcac
     8461 ttcgtggccg aggagcagga ctgacacgtg ctacgagatt tcgattccac cgccgccttc
     8521 tatgaaaggt tgggcttcgg aatcgttttc cgggacgccg gctggatgat cctccagcgc
     8581 ggggatctca tgctggagtt cttcgcccac cccaacttgt ttattgcagc ttataatggt
     8641 tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     8701 agttgtggtt tgtccaaact catcaatgta tcttatcatg tctgtatacc gtcgacctct
     8761 agctagagct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg ttatccgctc
     8821 acaattccac acaacatacg agccggaagc ataaagtgta aagcctgggg tgcctaatga
     8881 gtgagctaac tcacattaat tgcgttgcgc tcactgcccg ctttccagtc gggaaacctg
     8941 tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
     9001 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
     9061 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
     9121 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
     9181 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
     9241 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
     9301 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
     9361 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
     9421 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
     9481 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
     9541 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
     9601 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
     9661 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
     9721 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
     9781 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
     9841 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
     9901 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
     9961 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
    10021 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
    10081 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
    10141 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
    10201 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
    10261 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
    10321 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
    10381 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
    10441 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
    10501 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
    10561 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
    10621 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
    10681 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
    10741 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
    10801 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
    10861 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
    10921 cgaaaagtgc cacctgacgt cgacggatcg ggagatctcc cgatccccta tggtgcactc
    10981 tcagtacaat ctgctctgat gccgcatagt taagccagta tctgctccct gcttgtgtgt
    11041 tggaggtcgc tgagtagtgc gcgagcaaaa tttaagctac aacaaggcaa ggcttgaccg
    11101 acaattgcat gaagaatctg cttagggtta ggcgttttgc gctgcttcgc gatgtacggg
    11161 ccagatatac gcgttgacat tgattattga ctagttatta atagtaatca attacggggt
    11221 cattagttca tagcccatat atggagttcc gcgttacata acttacggta aatggcccgc
    11281 ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat gttcccatag
    11341 taacgccaat agggactttc cattgacg
//
