LOCUS       sequence-424327-        5970 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses mouse PIRH2.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5970)
  AUTHORS   Wu YQ, Zhang CS, Xiong J, Cai DQ, Wang CZ, Wang Y, Liu YH, Wang Y, 
            Li Y, Wu J, Wu J, Lan B, Wang X, Chen S, Cao X, Wei X, Hu HH, Guo H,
            Yu Y, Ghafoor A, Xie C, Wu Y, Xu Z, Zhang C, Zhu M, Huang X, Sun X, 
            Lin SY, Piao HL, Zhou J, Lin SC
  TITLE     Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine
            synthesis to p53 activation to control cell fate.
  JOURNAL   Cell Res. 2023 Nov;33(11):835-850. doi: 10.1038/s41422-023-00874-4. 
            Epub 2023 Sep 19.
  PUBMED    37726403
REFERENCE   2  (bases 1 to 5970)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41422-023-00874-4"; journalName: "Cell Res"; date: 
            "2023-11"; volume: "33"; issue: "11"; pages: "835-850"
COMMENT     Sequence Label: pCMV5 n-myc mPirh2
FEATURES             Location/Qualifiers
     source          1..5970
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     116..138
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     130..147
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     131..147
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        308..687
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        688..891
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     842..862
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             958..987
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     primer_bind     complement(2277..2293)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2277..2293)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     polyA_signal    2301..2923
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2500..2519)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(2947..2967)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2952..3281
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3132..3267
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(3319..3338)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(3320..3338)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(3352..3368)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(3352..3368)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(3365..3387)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    3376..3392
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3400..3406,3407..3424,3425..3430))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3445..3466
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(3583..3600)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3754..4342)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3834..3853)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4513..5304,5305..5373))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5136..5155
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5374..5478)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      5505..5960
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5592..5611)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5802..5823
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
       61 ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg gttttcccag
      121 tcacgacgtt gtaaaacgac ggccagtgcc aagctgatct atacattgaa tcaatattgg
      181 caattagcca tattagtcat tggttatata gcataaatca atattggcta ttggccattg
      241 catacgttgt atctatatca taatatgtac atttatattg gctcatgtcc aatatgaccg
      301 ccatgttgac attgattatt gactagttat taatagtaat caattacggg gtcattagtt
      361 catagcccat atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga
      421 ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca
      481 atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca
      541 gtacatcaag tgtatgatat gccaagtccg ccccctattg acgtcaatga cggtaaatgg
      601 cccgcctggc attatgccca gtacatgacc ttacgggact ttcctacttg gcagtacatc
      661 tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacac caatgggcgt
      721 ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt
      781 ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaataaccc cgccccgttg
      841 acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc tcgtttagtg
      901 aaccgtcaga attcagatct ggtaccacgc gtatcgataa gcttgatcat ggcagaggag
      961 caaaagctca tttctgaaga ggacttgttg cggaaacata tggcggcgac ggcgcgggaa
     1021 gatggcgtcc gcaacctggc gcaggggccg cggggctgcg agcactatga cagagcttgt
     1081 ctcctcaagg caccttgttg tgacaagctt tatacctgcc ggctgtgtca cgataccaat
     1141 gaagatcatc agctagatcg tttcaaagtc aaggaagtgc agtgcatcaa ctgtgaaaaa
     1201 ctccaacatg cccagcagac ttgtgaagac tgtagcacat tgtttggaga atattactgc
     1261 agtatttgcc acctgtttga caaagataag aggcagtacc actgtgagag ctgtgggatt
     1321 tgtaggattg gtccaaagga agattttttc cattgcttga aatgtaattt atgcctaacc
     1381 acgaatcttc gaggaaaaca caagtgtatt gaaaatgttt cccggcagaa ttgtccaata
     1441 tgcttggagg acattcacac ctcccgtgtt gttgctcacg tcttgccgtg tgggcatctc
     1501 ctacatagaa cgtgttatga agaaatgttg aaagaaggtt acagatgccc attgtgtatg
     1561 cattctgcct tagacatgac tcggtactgg agacaactag atactgaggt agcacagact
     1621 cccatgccat ccgaatacca gaacgtgact gttgacattc tctgcaatga ctgtaatgga
     1681 cgatccacag tccagttcca tatcttaggc atgaaatgta agctttgtga ctcctacaat
     1741 actgcccaag ctggaggccg tagagtgcca gtggatcagc agtgagaagc ctgtccttgg
     1801 ctagagaaca gctctgtgat ggagatgaag ctctgtaaaa gatcccagtg ttcgttcatc
     1861 tgcatcagag atgtttcata ccactgtcac aacagggggt cacagcgtga acacctgagg
     1921 attcaagatc tctggatgga aatgtaatag agcttactgg gtgattgtaa tagcgattac
     1981 tgggtgaagc ctctgagcgt gtgtagattg gaaattcctt tagtgtcaag ttttgaagcc
     2041 aatgatgtta gtcccagata tagtcacatt catcgaaata ttttatattt tcatggaact
     2101 ttaagtgata ctatgcacgc agagttaatt tcttgttctt gatagaacta cattcaattt
     2161 taactagtct ttgattttaa gggctgtact gtgattttta agttcttatt aaaatctaat
     2221 tacgtgtgca aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa actcgagatc
     2281 cactagttct agaggatccc gggtggcatc cctgtgaccc ctccccagtg cctctcctgg
     2341 ccctggaagt tgccactcca gtgcccacca gccttgtcct aataaaatta agttgcatca
     2401 ttttgtctga ctaggtgtcc ttctataata ttatggggtg gaggggggtg gtatggagca
     2461 aggggcaagt tgggaagaca acctgtaggg cctgcggggt ctattgggaa ccaagctgga
     2521 gtgcagtggc acaatcttgg ctcactgcaa tctccgcctc ctgggttcaa gcgattctcc
     2581 tgcctcagcc tcccgagttg ttgggattcc aggcatgcat gaccaggctc agctaatttt
     2641 tgtttttttg gtagagacgg ggtttcacca tattggccag gctggtctcc aactcctaat
     2701 ctcaggtgat ctacccacct tggcctccca aattgctggg attacaggcg tgaaccactg
     2761 ctcccttccc tgtccttctg attttaaaat aactatacca gcaggaggac gtccagacac
     2821 agcataggct acctggccat gcccaaccgg tgggacattt gagttgcttg cttggcactg
     2881 tcctctcatg cgttgggtcc actcagtaga tgcctgttga attgggtacg cggccagctt
     2941 ggctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc aggctcccca gcaggcagaa
     3001 gtatgcaaag catgcatctc aattagtcag caaccaggtg tggaaagtcc ccaggctccc
     3061 cagcaggcag aagtatgcaa agcatgcatc tcaattagtc agcaaccata gtcccgcccc
     3121 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3181 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3241 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctcctc gaggaactga
     3301 aaaaccagaa agttaattcc ctatagtgag tcgtattaaa ttcgtaatca tgtcatagct
     3361 gtttcctgtg tgaaattgtt atccgctcac aattccacac aacatacgag ccggaagcat
     3421 aaagtgtaaa gcctggggtg cctaatgagt gagctaactc acattaattg cgttgcgctc
     3481 actgcccgct ttccagtcgg gaaacctgtc gtgccagctg cattaatgaa tcggccaacg
     3541 cgcggggaga ggcggtttgc gtattgggcg ctcttccgct tcctcgctca ctgactcgct
     3601 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     3661 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     3721 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     3781 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     3841 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     3901 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     3961 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     4021 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     4081 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     4141 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     4201 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     4261 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     4321 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     4381 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     4441 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     4501 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     4561 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     4621 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     4681 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     4741 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     4801 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     4861 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     4921 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     4981 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     5041 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     5101 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     5161 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     5221 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     5281 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     5341 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     5401 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     5461 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg
     5521 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     5581 cctagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     5641 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     5701 cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac
     5761 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     5821 tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat
     5881 ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     5941 aatattaacg cttacaattt ccattcgcca
//
