LOCUS       sequence-424329-        9005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG epitope-tagged CeNLuc ""Blue"" BRET reporter in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Labra B, Parag-Sharma K, Powers JJ, Srivastava S, Walker JR, 
            Kirkland TA, Brennan CK, Prescher JA, Amelio AL
  TITLE     Optimized in vivo multispectral bioluminescent imaging of tumor 
            biology using engineered BRET reporters.
  JOURNAL   iScience. 2024 Aug 8;27(9):110655. doi: 10.1016/j.isci.2024.110655. 
            eCollection 2024 Sep 20.
  PUBMED    39252965
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-08-8"; volume: "27(9):110655. doi"; pages: " 
            10.1016/j.isci.2024.110655. eCollection 2024 Sep 20"
COMMENT     Sequence Label: pLenti_CMV:Flag-CeNLuc
FEATURES             Location/Qualifiers
     source          1..9005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(408..419)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             612..845
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    917..1038
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(954..973)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     1008..1027
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1078..1213
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1233..1252)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1234..1252)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1262..1279)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1262..1278)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1271..1293)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1420..1875
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1507..1526)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1717..1738
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1901..2005
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(2006..2074,2075..2866)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2224..2243)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3037..3625
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3526..3545
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3779..3796
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3913..3934
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3949..3954,3955..3972,3973..3979)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3987..4003
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3992..4014
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     4011..4027
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     4011..4027
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4046..4066
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4048..4066
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4094..4320
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4321..4501
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4548..4673
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5166..5399
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5584..5628
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5895..6011
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6034..6337
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6338..6541
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6492..6512
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6538..6562
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6621..6644
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(6645..6647,6648..7358)
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIKA
                     NFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(6687..6708)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(6948..6967)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7295..7316
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             7374..7889
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(7932..7952)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7937..8266
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8117..8252
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8276..8875
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8276..8295)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8772..8792
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(8942..9005,1..525)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(join(8995..9005,1..10))
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
       61 ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc
      121 ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt
      181 ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg
      241 ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg
      301 gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg ctgctcgcct
      361 gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc
      421 cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtcttcgcc
      481 ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga attcgagctc
      541 ggtaccttta agaccaatga cttacaaggc agctgtagat cttagccact ttttaaaaga
      601 aaagggggga ctggaagggc taattcactc ccaacgaaga caagatctgc tttttgcttg
      661 tactgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
      721 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
      781 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
      841 tagcagtagt agttcatgtc atcttattat tcagtattta taacttgcaa agaaatgaat
      901 atcagagagt gagaggaact tgtttattgc agcttataat ggttacaaat aaagcaatag
      961 catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg gtttgtccaa
     1021 actcatcaat gtatcttatc atgtctggct ctagctatcc cgcccctaac tccgcccatc
     1081 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     1141 atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     1201 ttttttggag gcctagggac gtacccaatt cgccctatag tgagtcgtat tacgcgcgct
     1261 cactggccgt cgttttacaa cgtcgtgact gggaaaaccc tggcgttacc caacttaatc
     1321 gccttgcagc acatccccct ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc
     1381 gcccttccca acagttgcgc agcctgaatg gcgaatggga cgcgccctgt agcggcgcat
     1441 taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc agcgccctag
     1501 cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc tttccccgtc
     1561 aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg cacctcgacc
     1621 ccaaaaaact tgattagggt gatggttcac gtagtgggcc atcgccctga tagacggttt
     1681 ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc caaactggaa
     1741 caacactcaa ccctatctcg gtctattctt ttgatttata agggattttg ccgatttcgg
     1801 cctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt aacaaaatat
     1861 taacgcttac aatttaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
     1921 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
     1981 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
     2041 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
     2101 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
     2161 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
     2221 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
     2281 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
     2341 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
     2401 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
     2461 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
     2521 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
     2581 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
     2641 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
     2701 atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc cagatggtaa
     2761 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
     2821 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
     2881 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
     2941 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
     3001 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
     3061 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
     3121 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
     3181 tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
     3241 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
     3301 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
     3361 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
     3421 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
     3481 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
     3541 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
     3601 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
     3661 cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt ctgtggataa
     3721 ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga ccgagcgcag
     3781 cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc tccccgcgcg
     3841 ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag cgggcagtga
     3901 gcgcaacgca attaatgtga gttagctcac tcattaggca ccccaggctt tacactttat
     3961 gcttccggct cgtatgttgt gtggaattgt gagcggataa caatttcaca caggaaacag
     4021 ctatgaccat gattacgcca agcgcgcaat taaccctcac taaagggaac aaaagctgga
     4081 gctgcaagct taatgtagtc ttatgcaata ctcttgtagt cttgcaacat ggtaacgatg
     4141 agttagcaac atgccttaca aggagagaaa aagcaccgtg catgccgatt ggtggaagta
     4201 aggtggtacg atcgtgcctt attaggaagg caacagacgg gtctgacatg gattggacga
     4261 accactgaat tgccgcattg cagagatatt gtatttaagt gcctagctcg atacataaac
     4321 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     4381 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     4441 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     4501 agtggcgccc gaacagggac ttgaaagcga aagggaaacc agaggagctc tctcgacgca
     4561 ggactcggct tgctgaagcg cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc
     4621 caaaaatttt gactagcgga ggctagaagg agagagatgg gtgcgagagc gtcagtatta
     4681 agcgggggag aattagatcg cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa
     4741 aatataaatt aaaacatata gtatgggcaa gcagggagct agaacgattc gcagttaatc
     4801 ctggcctgtt agaaacatca gaaggctgta gacaaatact gggacagcta caaccatccc
     4861 ttcagacagg atcagaagaa cttagatcat tatataatac agtagcaacc ctctattgtg
     4921 tgcatcaaag gatagagata aaagacacca aggaagcttt agacaagata gaggaagagc
     4981 aaaacaaaag taagaccacc gcacagcaag cggccgctga tcttcagacc tggaggagga
     5041 gatatgaggg acaattggag aagtgaatta tataaatata aagtagtaaa aattgaacca
     5101 ttaggagtag cacccaccaa ggcaaagaga agagtggtgc agagagaaaa aagagcagtg
     5161 ggaataggag ctttgttcct tgggttcttg ggagcagcag gaagcactat gggcgcagcg
     5221 tcaatgacgc tgacggtaca ggccagacaa ttattgtctg gtatagtgca gcagcagaac
     5281 aatttgctga gggctattga ggcgcaacag catctgttgc aactcacagt ctggggcatc
     5341 aagcagctcc aggcaagaat cctggctgtg gaaagatacc taaaggatca acagctcctg
     5401 gggatttggg gttgctctgg aaaactcatt tgcaccactg ctgtgccttg gaatgctagt
     5461 tggagtaata aatctctgga acagatttgg aatcacacga cctggatgga gtgggacaga
     5521 gaaattaaca attacacaag cttaatacac tccttaattg aagaatcgca aaaccagcaa
     5581 gaaaagaatg aacaagaatt attggaatta gataaatggg caagtttgtg gaattggttt
     5641 aacataacaa attggctgtg gtatataaaa ttattcataa tgatagtagg aggcttggta
     5701 ggtttaagaa tagtttttgc tgtactttct atagtgaata gagttaggca gggatattca
     5761 ccattatcgt ttcagaccca cctcccaacc ccgaggggac ccgacaggcc cgaaggaata
     5821 gaagaagaag gtggagagag agacagagac agatccattc gattagtgaa cggatctcga
     5881 cggtatcggt taacttttaa aagaaaaggg gggattgggg ggtacagtgc aggggaaaga
     5941 atagtagaca taatagcaac agacatacaa actaaagaat tacaaaaaca aattacaaaa
     6001 ttcaaaattt tatcgataag cttgggagtt ccgcgttaca taacttacgg taaatggccc
     6061 gcctggctga ccgcccaacg acccccgccc attgacgtca ataatgacgt atgttcccat
     6121 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
     6181 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
     6241 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
     6301 gcagtacatc tacgtattag tcatcgctat taccatggtg atgcggtttt ggcagtacat
     6361 caatgggcgt ggatagcggt ttgactcacg gggatttcca agtctccacc ccattgacgt
     6421 caatgggagt ttgttttggc accaaaatca acgggacttt ccaaaatgtc gtaacaactc
     6481 cgccccattg acgcaaatgg gcggtaggcg tgtacggtgg gaggtctata taagcagagc
     6541 tcgtttagtg aaccgtcaga tcgcctggag acgccatcca cgctgttttg acctccatag
     6601 aagacaccga ctctagaatg gactacaagg acgacgatga caaggtgagc aagggcgagg
     6661 agctgttcac cggggtggtg cccatcctgg tcgagctgga cggcgacgta aacggccaca
     6721 agttcagcgt gtccggcgag ggcgagggcg atgccaccta cggcaagctg accctgaagt
     6781 tcatctgcac caccggcaag ctgcccgtgc cctggcccac cctcgtgacc accctgagct
     6841 ggggcgtgca gtgcttcgcc cgctaccccg accacatgaa gcagcacgac ttcttcaagt
     6901 ccgccatgcc cgaaggctac gtccaggagc gcaccatctt cttcaaggac gacggcaact
     6961 acaagacccg cgccgaggtg aagttcgagg gcgacaccct ggtgaaccgc atcgagctga
     7021 agggcatcga cttcaaggag gacggcaaca tcctggggca caagctggag tacaacgcca
     7081 tccacggcaa cgtctatatc accgccgaca agcagaagaa cggcatcaag gccaacttcg
     7141 gcctcaactg caacatcgag gacggcagcg tgcagctcgc cgaccactac cagcagaaca
     7201 cccccatcgg cgacggcccc gtgctgctgc ccgacaacca ctacctgagc acccagtcca
     7261 agctgagcaa agaccccaac gagaagcgcg atcacatggt cctgctggag ttcgtgaccg
     7321 ccgccgggat cactctcggc atggacgagc tgtacaagga tatctccgga ggtatggtct
     7381 tcacactcga agatttcgtt ggggactggc gacagacagc cggctacaac ctggaccaag
     7441 tccttgaaca gggaggtgtg tccagtttgt ttcagaatct cggggtgtcc gtaactccga
     7501 tccaaaggat tgtcctgagc ggtgaaaatg ggctgaagat cgacatccat gtcatcatcc
     7561 cgtatgaagg tctgagcggc gaccaaatgg gccagatcga aaaaattttt aaggtggtgt
     7621 accctgtgga tgatcatcac tttaaggtga tcctgcacta tggcacactg gtaatcgacg
     7681 gggttacgcc gaacatgatc gactatttcg gacggccgta tgaaggcatc gccgtgttcg
     7741 acggcaaaaa gatcactgta acagggaccc tgtggaacgg caacaaaatt atcgacgagc
     7801 gcctgatcaa ccccgacggc tccctgctgt tccgagtaac catcaacgga gtgaccggct
     7861 ggcggctgtg cgaacgcatt ctggcgtaag gatccggact agttaacgcg tcgaccagtg
     7921 gtcgaccctg tggaatgtgt gtcagttagg gtgtggaaag tccccaggct ccccagcagg
     7981 cagaagtatg caaagcatgc atctcaatta gtcagcaacc aggtgtggaa agtccccagg
     8041 ctccccagca ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccatagtccc
     8101 gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt ctccgcccca
     8161 tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct ctgagctatt
     8221 ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc ttaccatgac
     8281 cgagtacaag cccacggtgc gcctcgccac ccgcgacgac gtccccaggg ccgtacgcac
     8341 cctcgccgcc gcgttcgccg actaccccgc cacgcgccac accgtcgatc cggaccgcca
     8401 catcgagcgg gtcaccgagc tgcaagaact cttcctcacg cgcgtcgggc tcgacatcgg
     8461 caaggtgtgg gtcgcggacg acggcgccgc ggtggcggtc tggaccacgc cggagagcgt
     8521 cgaagcgggg gcggtgttcg ccgagatcgg cccgcgcatg gccgagttga gcggttcccg
     8581 gctggccgcg cagcaacaga tggaaggcct cctggcgccg caccggccca aggagcccgc
     8641 gtggttcctg gccaccgtcg gcgtctcgcc cgaccaccag ggcaagggtc tgggcagcgc
     8701 cgtcgtgctc cccggagtgg aggcggccga gcgcgccggg gtgcccgcct tcctggagac
     8761 ctccgcgccc cgcaacctcc ccttctacga gcggctcggc ttcaccgtca ccgccgacgt
     8821 cgaggtgccc gaaggaccgc gcacctggtg catgacccgc aagcccggtg cctgacgccc
     8881 gccccacgac ccgcagcgcc cgaccgaaag gagcgcacga ccccatgcat cgccagtcga
     8941 caatcaacct ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc
     9001 tcctt
//
