LOCUS       sequence-424330-        9005 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG epitope-tagged GpNLuc ""Green"" BRET reporter in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9005)
  AUTHORS   Labra B, Parag-Sharma K, Powers JJ, Srivastava S, Walker JR, 
            Kirkland TA, Brennan CK, Prescher JA, Amelio AL
  TITLE     Optimized in vivo multispectral bioluminescent imaging of tumor 
            biology using engineered BRET reporters.
  JOURNAL   iScience. 2024 Aug 8;27(9):110655. doi: 10.1016/j.isci.2024.110655. 
            eCollection 2024 Sep 20.
  PUBMED    39252965
REFERENCE   2  (bases 1 to 9005)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-08-8"; volume: "27(9):110655. doi"; pages: " 
            10.1016/j.isci.2024.110655. eCollection 2024 Sep 20"
COMMENT     Sequence Label: pLenti_CMV:Flag-GpNLuc
FEATURES             Location/Qualifiers
     source          1..9005
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     185..202
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    319..340
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(355..360,361..378,379..385)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    393..409
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     398..420
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     417..433
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     417..433
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     452..472
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        454..472
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        500..726
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             727..907
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    954..1079
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1572..1805
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1990..2034
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2301..2417
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2440..2743
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2744..2947
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2898..2918
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2944..2968
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3027..3050
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(3051..3053,3054..3764)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     CDS             join(3051..3053,3054..3764)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(3093..3114)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3354..3373)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3701..3722
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             3780..4295
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
     primer_bind     complement(4338..4358)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4343..4672
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4523..4658
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4682..5281
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4682..4701)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5178..5198
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5348..5936
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5401..5421)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5819..5830)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6023..6256
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6328..6449
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6365..6384)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6419..6438
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6489..6624
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6644..6663)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6645..6663)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6673..6690)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6673..6689)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6682..6704)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6831..7286
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6918..6937)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7128..7149
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7312..7416
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7417..7485,7486..8277)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7635..7654)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8448..9005,1..31)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8937..8956
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 atgctcgtca ggggggcgga gcctatggaa aaacgccagc aacgcggcct ttttacggtt
       61 cctggccttt tgctggcctt ttgctcacat gttctttcct gcgttatccc ctgattctgt
      121 ggataaccgt attaccgcct ttgagtgagc tgataccgct cgccgcagcc gaacgaccga
      181 gcgcagcgag tcagtgagcg aggaagcgga agagcgccca atacgcaaac cgcctctccc
      241 cgcgcgttgg ccgattcatt aatgcagctg gcacgacagg tttcccgact ggaaagcggg
      301 cagtgagcgc aacgcaatta atgtgagtta gctcactcat taggcacccc aggctttaca
      361 ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc ggataacaat ttcacacagg
      421 aaacagctat gaccatgatt acgccaagcg cgcaattaac cctcactaaa gggaacaaaa
      481 gctggagctg caagcttaat gtagtcttat gcaatactct tgtagtcttg caacatggta
      541 acgatgagtt agcaacatgc cttacaagga gagaaaaagc accgtgcatg ccgattggtg
      601 gaagtaaggt ggtacgatcg tgccttatta ggaaggcaac agacgggtct gacatggatt
      661 ggacgaacca ctgaattgcc gcattgcaga gatattgtat ttaagtgcct agctcgatac
      721 ataaacgggt ctctctggtt agaccagatc tgagcctggg agctctctgg ctaactaggg
      781 aacccactgc ttaagcctca ataaagcttg ccttgagtgc ttcaagtagt gtgtgcccgt
      841 ctgttgtgtg actctggtaa ctagagatcc ctcagaccct tttagtcagt gtggaaaatc
      901 tctagcagtg gcgcccgaac agggacttga aagcgaaagg gaaaccagag gagctctctc
      961 gacgcaggac tcggcttgct gaagcgcgca cggcaagagg cgaggggcgg cgactggtga
     1021 gtacgccaaa aattttgact agcggaggct agaaggagag agatgggtgc gagagcgtca
     1081 gtattaagcg ggggagaatt agatcgcgat gggaaaaaat tcggttaagg ccagggggaa
     1141 agaaaaaata taaattaaaa catatagtat gggcaagcag ggagctagaa cgattcgcag
     1201 ttaatcctgg cctgttagaa acatcagaag gctgtagaca aatactggga cagctacaac
     1261 catcccttca gacaggatca gaagaactta gatcattata taatacagta gcaaccctct
     1321 attgtgtgca tcaaaggata gagataaaag acaccaagga agctttagac aagatagagg
     1381 aagagcaaaa caaaagtaag accaccgcac agcaagcggc cgctgatctt cagacctgga
     1441 ggaggagata tgagggacaa ttggagaagt gaattatata aatataaagt agtaaaaatt
     1501 gaaccattag gagtagcacc caccaaggca aagagaagag tggtgcagag agaaaaaaga
     1561 gcagtgggaa taggagcttt gttccttggg ttcttgggag cagcaggaag cactatgggc
     1621 gcagcgtcaa tgacgctgac ggtacaggcc agacaattat tgtctggtat agtgcagcag
     1681 cagaacaatt tgctgagggc tattgaggcg caacagcatc tgttgcaact cacagtctgg
     1741 ggcatcaagc agctccaggc aagaatcctg gctgtggaaa gatacctaaa ggatcaacag
     1801 ctcctgggga tttggggttg ctctggaaaa ctcatttgca ccactgctgt gccttggaat
     1861 gctagttgga gtaataaatc tctggaacag atttggaatc acacgacctg gatggagtgg
     1921 gacagagaaa ttaacaatta cacaagctta atacactcct taattgaaga atcgcaaaac
     1981 cagcaagaaa agaatgaaca agaattattg gaattagata aatgggcaag tttgtggaat
     2041 tggtttaaca taacaaattg gctgtggtat ataaaattat tcataatgat agtaggaggc
     2101 ttggtaggtt taagaatagt ttttgctgta ctttctatag tgaatagagt taggcaggga
     2161 tattcaccat tatcgtttca gacccacctc ccaaccccga ggggacccga caggcccgaa
     2221 ggaatagaag aagaaggtgg agagagagac agagacagat ccattcgatt agtgaacgga
     2281 tctcgacggt atcggttaac ttttaaaaga aaagggggga ttggggggta cagtgcaggg
     2341 gaaagaatag tagacataat agcaacagac atacaaacta aagaattaca aaaacaaatt
     2401 acaaaattca aaattttatc gataagcttg ggagttccgc gttacataac ttacggtaaa
     2461 tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt
     2521 tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt atttacggta
     2581 aactgcccac ttggcagtac atcaagtgta tcatatgcca agtacgcccc ctattgacgt
     2641 caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttat gggactttcc
     2701 tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc ggttttggca
     2761 gtacatcaat gggcgtggat agcggtttga ctcacgggga tttccaagtc tccaccccat
     2821 tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa aatgtcgtaa
     2881 caactccgcc ccattgacgc aaatgggcgg taggcgtgta cggtgggagg tctatataag
     2941 cagagctcgt ttagtgaacc gtcagatcgc ctggagacgc catccacgct gttttgacct
     3001 ccatagaaga caccgactct agaatggact acaaggacga cgatgacaag gtgagcaagg
     3061 gcgaggagct gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtaaacg
     3121 gccacaagtt cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc
     3181 tgaagttcat ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccaccc
     3241 tgacctacgg cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct
     3301 tcaagtccgc catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg
     3361 gcaactacaa gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg
     3421 agctgaaggg catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca
     3481 actacaacag ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga
     3541 acttcaagat ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc
     3601 agaacacccc catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc
     3661 agtccgccct gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg
     3721 tgaccgccgc cgggatcact ctcggcatgg acgagctgta caaggatatc tccggaggta
     3781 tggtcttcac actcgaagat ttcgttgggg actggcgaca gacagccggc tacaacctgg
     3841 accaagtcct tgaacaggga ggtgtgtcca gtttgtttca gaatctcggg gtgtccgtaa
     3901 ctccgatcca aaggattgtc ctgagcggtg aaaatgggct gaagatcgac atccatgtca
     3961 tcatcccgta tgaaggtctg agcggcgacc aaatgggcca gatcgaaaaa atttttaagg
     4021 tggtgtaccc tgtggatgat catcacttta aggtgatcct gcactatggc acactggtaa
     4081 tcgacggggt tacgccgaac atgatcgact atttcggacg gccgtatgaa ggcatcgccg
     4141 tgttcgacgg caaaaagatc actgtaacag ggaccctgtg gaacggcaac aaaattatcg
     4201 acgagcgcct gatcaacccc gacggctccc tgctgttccg agtaaccatc aacggagtga
     4261 ccggctggcg gctgtgcgaa cgcattctgg cgtaaggatc cggactagtt aacgcgtcga
     4321 ccagtggtcg accctgtgga atgtgtgtca gttagggtgt ggaaagtccc caggctcccc
     4381 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccaggt gtggaaagtc
     4441 cccaggctcc ccagcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccat
     4501 agtcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg cccattctcc
     4561 gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct cggcctctga
     4621 gctattccag aagtagtgag gaggcttttt tggaggccta ggcttttgca aaaagcttac
     4681 catgaccgag tacaagccca cggtgcgcct cgccacccgc gacgacgtcc ccagggccgt
     4741 acgcaccctc gccgccgcgt tcgccgacta ccccgccacg cgccacaccg tcgatccgga
     4801 ccgccacatc gagcgggtca ccgagctgca agaactcttc ctcacgcgcg tcgggctcga
     4861 catcggcaag gtgtgggtcg cggacgacgg cgccgcggtg gcggtctgga ccacgccgga
     4921 gagcgtcgaa gcgggggcgg tgttcgccga gatcggcccg cgcatggccg agttgagcgg
     4981 ttcccggctg gccgcgcagc aacagatgga aggcctcctg gcgccgcacc ggcccaagga
     5041 gcccgcgtgg ttcctggcca ccgtcggcgt ctcgcccgac caccagggca agggtctggg
     5101 cagcgccgtc gtgctccccg gagtggaggc ggccgagcgc gccggggtgc ccgccttcct
     5161 ggagacctcc gcgccccgca acctcccctt ctacgagcgg ctcggcttca ccgtcaccgc
     5221 cgacgtcgag gtgcccgaag gaccgcgcac ctggtgcatg acccgcaagc ccggtgcctg
     5281 acgcccgccc cacgacccgc agcgcccgac cgaaaggagc gcacgacccc atgcatcgcc
     5341 agtcgacaat caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta
     5401 tgttgctcct tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc
     5461 ttcccgtatg gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga
     5521 ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac
     5581 ccccactggt tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc
     5641 cctccctatt gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc
     5701 tcggctgttg ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg
     5761 gctgctcgcc tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc
     5821 ggccctcaat ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc
     5881 gcgtcttcgc cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg
     5941 aattcgagct cggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac
     6001 tttttaaaag aaaagggggg actggaaggg ctaattcact cccaacgaag acaagatctg
     6061 ctttttgctt gtactgggtc tctctggtta gaccagatct gagcctggga gctctctggc
     6121 taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct tcaagtagtg
     6181 tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt ttagtcagtg
     6241 tggaaaatct ctagcagtag tagttcatgt catcttatta ttcagtattt ataacttgca
     6301 aagaaatgaa tatcagagag tgagaggaac ttgtttattg cagcttataa tggttacaaa
     6361 taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     6421 ggtttgtcca aactcatcaa tgtatcttat catgtctggc tctagctatc ccgcccctaa
     6481 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
     6541 taattttttt tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt
     6601 agtgaggagg cttttttgga ggcctaggga cgtacccaat tcgccctata gtgagtcgta
     6661 ttacgcgcgc tcactggccg tcgttttaca acgtcgtgac tgggaaaacc ctggcgttac
     6721 ccaacttaat cgccttgcag cacatccccc tttcgccagc tggcgtaata gcgaagaggc
     6781 ccgcaccgat cgcccttccc aacagttgcg cagcctgaat ggcgaatggg acgcgccctg
     6841 tagcggcgca ttaagcgcgg cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc
     6901 cagcgcccta gcgcccgctc ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg
     6961 ctttccccgt caagctctaa atcgggggct ccctttaggg ttccgattta gtgctttacg
     7021 gcacctcgac cccaaaaaac ttgattaggg tgatggttca cgtagtgggc catcgccctg
     7081 atagacggtt tttcgccctt tgacgttgga gtccacgttc tttaatagtg gactcttgtt
     7141 ccaaactgga acaacactca accctatctc ggtctattct tttgatttat aagggatttt
     7201 gccgatttcg gcctattggt taaaaaatga gctgatttaa caaaaattta acgcgaattt
     7261 taacaaaata ttaacgctta caatttaggt ggcacttttc ggggaaatgt gcgcggaacc
     7321 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
     7381 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
     7441 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
     7501 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
     7561 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
     7621 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
     7681 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
     7741 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
     7801 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
     7861 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
     7921 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
     7981 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
     8041 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
     8101 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
     8161 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
     8221 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
     8281 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
     8341 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
     8401 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
     8461 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
     8521 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
     8581 ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta
     8641 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
     8701 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
     8761 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
     8821 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
     8881 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
     8941 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
     9001 ttgtg
//
