LOCUS       sequence-424331-        9641 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG epitope-tagged Antares ""Orange"" BRET reporter in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9641)
  AUTHORS   Labra B, Parag-Sharma K, Powers JJ, Srivastava S, Walker JR, 
            Kirkland TA, Brennan CK, Prescher JA, Amelio AL
  TITLE     Optimized in vivo multispectral bioluminescent imaging of tumor 
            biology using engineered BRET reporters.
  JOURNAL   iScience. 2024 Aug 8;27(9):110655. doi: 10.1016/j.isci.2024.110655. 
            eCollection 2024 Sep 20.
  PUBMED    39252965
REFERENCE   2  (bases 1 to 9641)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-08-8"; volume: "27(9):110655. doi"; pages: " 
            10.1016/j.isci.2024.110655. eCollection 2024 Sep 20"
COMMENT     Sequence Label: pLenti_CMV:Flag-Antares
FEATURES             Location/Qualifiers
     source          1..9641
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        301..504
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     455..475
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     501..525
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             584..607
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             1289..1795
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="FTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQRIV
                     LSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGVTP
                     NMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGWRL
                     CERILA"
     primer_bind     complement(2531..2551)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        2536..2865
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      2716..2851
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             2875..3474
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(2875..2894)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     3371..3391
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    3541..4129
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(3594..3614)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(4012..4023)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             4216..4449
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    4521..4642
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4558..4577)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4612..4631
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      4682..4817
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4837..4856)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4838..4856)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4866..4883)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(4866..4882)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4875..4897)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      5024..5479
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5111..5130)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     5321..5342
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        5505..5609
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5610..5678,5679..6470)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5828..5847)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6641..7229
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7130..7149
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7383..7400
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7517..7538
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7553..7558,7559..7576,7577..7583)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7591..7607
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7596..7618
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7615..7631
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7615..7631
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     7650..7670
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        7652..7670
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        7698..7924
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             7925..8105
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8152..8277
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8770..9003
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9188..9232
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9499..9615
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        join(9638..9641,1..300)
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
ORIGIN
        1 acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg
       61 tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg
      121 gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt
      181 acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg
      241 accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg
      301 gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc acggggattt
      361 ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa tcaacgggac
      421 tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag gcgtgtacgg
      481 tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg gagacgccat
      541 ccacgctgtt ttgacctcca tagaagacac cgactctaga atggactaca aggacgacga
      601 tgacaaggtg agcaagggcg aggagctgat caaggagaac atgagaagca agctgtacct
      661 ggaaggcagc gtgaacggcc accagttcaa gtgcacccac gaaggggagg gcaagcccta
      721 cgagggcaag cagaccaaca ggatcaaggt ggtggaggga ggccccctgc cgttcgcatt
      781 cgacatcctg gccacccact ttatgtacgg gagcaaggtg ttcatcaagt accccgccga
      841 cctccccgat tattttaagc agtccttccc tgagggcttc acatgggaga gagtcatggt
      901 gttcgaagac gggggcgtgc tgaccgccac ccaggacacc agcctccagg acggcgagct
      961 catctacaac gtcaaggtca gaggggtgaa cttcccagcc aacggccccg tgatgcagaa
     1021 gaaaacactg ggctgggagc ccagcaccga gaccatgtac cccgctgacg gcggcctgga
     1081 aggcagatgc gacaaggccc tgaagctcgt gggcgggggc cacctgcacg tcaacttcaa
     1141 gaccacatac aagtccaaga aacccgtgaa gatgcccggc gtccactacg tggaccgcag
     1201 actggaaaga atcaaggagg ccgacaacga gacctacgtc gagcagtacg agcacgctgt
     1261 ggccagatac tccaacctgg gcggaggctt cacactcgaa gatttcgttg gggactggcg
     1321 acagacagcc ggctacaacc tggaccaagt ccttgaacag ggaggtgtgt ccagtttgtt
     1381 tcagaatctc ggggtgtccg taactccgat ccaaaggatt gtcctgagcg gtgaaaatgg
     1441 gctgaagatc gacatccatg tcatcatccc gtatgaaggt ctgagcggcg accaaatggg
     1501 ccagatcgaa aaaattttta aggtggtgta ccctgtggat gatcatcact ttaaggtgat
     1561 cctgcactat ggcacactgg taatcgacgg ggttacgccg aacatgatcg actatttcgg
     1621 acggccgtat gaaggcatcg ccgtgttcga cggcaaaaag atcactgtaa cagggaccct
     1681 gtggaacggc aacaaaatta tcgacgagcg cctgatcaac cccgacggct ccctgctgtt
     1741 ccgagtaacc atcaacggag tgaccggctg gcggctgtgc gaacgcattc tggcgaggca
     1801 cgagctgatc aaggagaaca tgagaagcaa gctgtacctg gaaggcagcg tgaacggcca
     1861 ccagttcaag tgcacccacg aaggggaggg caagccctac gagggcaagc agaccaacag
     1921 gatcaaggtg gtggagggag gccccctgcc gttcgcattc gacatcctgg ccacccactt
     1981 tatgtacggg agcaaggtgt tcatcaagta ccccgccgac ctccccgatt attttaagca
     2041 gtccttccct gagggcttca catgggagag agtcatggtg ttcgaagacg ggggcgtgct
     2101 gaccgccacc caggacacca gcctccagga cggcgagctc atctacaacg tcaaggtcag
     2161 aggggtgaac ttcccagcca acggccccgt gatgcagaag aaaacactgg gctgggagcc
     2221 cagcaccgag accatgtacc ccgctgacgg cggcctggaa ggcagatgcg acaaggccct
     2281 gaagctcgtg ggcgggggcc acctgcacgt caacttcaag accacataca agtccaagaa
     2341 acccgtgaag atgcccggcg tccactacgt ggaccgcaga ctggaaagaa tcaaggaggc
     2401 cgacaacgag acctacgtcg agcagtacga gcacgctgtg gccagatact ccaacctggg
     2461 cggaggcatg gacgagctgt acaagtaagg atccggacta gttaacgcgt cgaccagtgg
     2521 tcgaccctgt ggaatgtgtg tcagttaggg tgtggaaagt ccccaggctc cccagcaggc
     2581 agaagtatgc aaagcatgca tctcaattag tcagcaacca ggtgtggaaa gtccccaggc
     2641 tccccagcag gcagaagtat gcaaagcatg catctcaatt agtcagcaac catagtcccg
     2701 cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc tccgccccat
     2761 ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc tgagctattc
     2821 cagaagtagt gaggaggctt ttttggaggc ctaggctttt gcaaaaagct taccatgacc
     2881 gagtacaagc ccacggtgcg cctcgccacc cgcgacgacg tccccagggc cgtacgcacc
     2941 ctcgccgccg cgttcgccga ctaccccgcc acgcgccaca ccgtcgatcc ggaccgccac
     3001 atcgagcggg tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc
     3061 aaggtgtggg tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc
     3121 gaagcggggg cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg
     3181 ctggccgcgc agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg
     3241 tggttcctgg ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc
     3301 gtcgtgctcc ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc
     3361 tccgcgcccc gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc
     3421 gaggtgcccg aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctgacgcccg
     3481 ccccacgacc cgcagcgccc gaccgaaagg agcgcacgac cccatgcatc gccagtcgac
     3541 aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa ctatgttgct
     3601 ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat tgcttcccgt
     3661 atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta tgaggagttg
     3721 tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc aacccccact
     3781 ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt ccccctccct
     3841 attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg ggctcggctg
     3901 ttgggcactg acaattccgt ggtgttgtcg gggaagctga cgtcctttcc atggctgctc
     3961 gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc ttcggccctc
     4021 aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct tccgcgtctt
     4081 cgccttcgcc ctcagacgag tcggatctcc ctttgggccg cctccccgcc tggaattcga
     4141 gctcggtacc tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa
     4201 aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat ctgctttttg
     4261 cttgtactgg gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag
     4321 ggaacccact gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc
     4381 gtctgttgtg tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa
     4441 tctctagcag tagtagttca tgtcatctta ttattcagta tttataactt gcaaagaaat
     4501 gaatatcaga gagtgagagg aacttgttta ttgcagctta taatggttac aaataaagca
     4561 atagcatcac aaatttcaca aataaagcat ttttttcact gcattctagt tgtggtttgt
     4621 ccaaactcat caatgtatct tatcatgtct ggctctagct atcccgcccc taactccgcc
     4681 catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt
     4741 ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga agtagtgagg
     4801 aggctttttt ggaggcctag ggacgtaccc aattcgccct atagtgagtc gtattacgcg
     4861 cgctcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     4921 aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     4981 gatcgccctt cccaacagtt gcgcagcctg aatggcgaat gggacgcgcc ctgtagcggc
     5041 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     5101 ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     5161 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     5221 gaccccaaaa aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     5281 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     5341 ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     5401 tcggcctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     5461 atattaacgc ttacaattta ggtggcactt ttcggggaaa tgtgcgcgga acccctattt
     5521 gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa
     5581 tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta
     5641 ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag
     5701 taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca
     5761 gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta
     5821 aagttctgct atgtggcgcg gtattatccc gtattgacgc cgggcaagag caactcggtc
     5881 gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc
     5941 ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca
     6001 ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc
     6061 acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca
     6121 taccaaacga cgagcgtgac accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac
     6181 tattaactgg cgaactactt actctagctt cccggcaaca attaatagac tggatggagg
     6241 cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg
     6301 ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg
     6361 gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac
     6421 gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc
     6481 aagtttactc atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct
     6541 aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc
     6601 actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc
     6661 gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg
     6721 atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa
     6781 atactgttct tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc
     6841 ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt
     6901 gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa
     6961 cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc
     7021 tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc
     7081 cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct
     7141 ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat
     7201 gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc
     7261 tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg
     7321 ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc
     7381 gcagcgagtc agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg
     7441 cgcgttggcc gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca
     7501 gtgagcgcaa cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact
     7561 ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa
     7621 acagctatga ccatgattac gccaagcgcg caattaaccc tcactaaagg gaacaaaagc
     7681 tggagctgca agcttaatgt agtcttatgc aatactcttg tagtcttgca acatggtaac
     7741 gatgagttag caacatgcct tacaaggaga gaaaaagcac cgtgcatgcc gattggtgga
     7801 agtaaggtgg tacgatcgtg ccttattagg aaggcaacag acgggtctga catggattgg
     7861 acgaaccact gaattgccgc attgcagaga tattgtattt aagtgcctag ctcgatacat
     7921 aaacgggtct ctctggttag accagatctg agcctgggag ctctctggct aactagggaa
     7981 cccactgctt aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct
     8041 gttgtgtgac tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc
     8101 tagcagtggc gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga
     8161 cgcaggactc ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt
     8221 acgccaaaaa ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt
     8281 attaagcggg ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag
     8341 aaaaaatata aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt
     8401 aatcctggcc tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca
     8461 tcccttcaga caggatcaga agaacttaga tcattatata atacagtagc aaccctctat
     8521 tgtgtgcatc aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa
     8581 gagcaaaaca aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg
     8641 aggagatatg agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga
     8701 accattagga gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc
     8761 agtgggaata ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc
     8821 agcgtcaatg acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca
     8881 gaacaatttg ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg
     8941 catcaagcag ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct
     9001 cctggggatt tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc
     9061 tagttggagt aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga
     9121 cagagaaatt aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca
     9181 gcaagaaaag aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg
     9241 gtttaacata acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt
     9301 ggtaggttta agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata
     9361 ttcaccatta tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg
     9421 aatagaagaa gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc
     9481 tcgacggtat cggttaactt ttaaaagaaa aggggggatt ggggggtaca gtgcagggga
     9541 aagaatagta gacataatag caacagacat acaaactaaa gaattacaaa aacaaattac
     9601 aaaattcaaa attttatcga taagcttggg agttccgcgt t
//
