LOCUS       sequence-424332-        6488 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  expresses human TIP60.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6488)
  AUTHORS   Wu YQ, Zhang CS, Xiong J, Cai DQ, Wang CZ, Wang Y, Liu YH, Wang Y, 
            Li Y, Wu J, Wu J, Lan B, Wang X, Chen S, Cao X, Wei X, Hu HH, Guo H,
            Yu Y, Ghafoor A, Xie C, Wu Y, Xu Z, Zhang C, Zhu M, Huang X, Sun X, 
            Lin SY, Piao HL, Zhou J, Lin SC
  TITLE     Low glucose metabolite 3-phosphoglycerate switches PHGDH from serine
            synthesis to p53 activation to control cell fate.
  JOURNAL   Cell Res. 2023 Nov;33(11):835-850. doi: 10.1038/s41422-023-00874-4. 
            Epub 2023 Sep 19.
  PUBMED    37726403
REFERENCE   2  (bases 1 to 6488)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/s41422-023-00874-4"; journalName: "Cell Res"; date: 
            "2023-11"; volume: "33"; issue: "11"; pages: "835-850"
COMMENT     Sequence Label: pCDNA3.3 N-MYC TIP60
FEATURES             Location/Qualifiers
     source          1..6488
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     112..133
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     396..418
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     410..427
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     411..427
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     enhancer        588..967
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        968..1171
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     1122..1142
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     CDS             1238..1267
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     CDS             complement(2889..2900)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     polyA_signal    3099..3721
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(3298..3317)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     primer_bind     complement(3745..3765)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3750..4079
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      3930..4065
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(4117..4136)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(4118..4136)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(4150..4166)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4150..4166)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(4163..4185)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    4174..4190
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(4198..4204,4205..4222,4223..4228))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    4243..4264
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(4381..4398)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4552..5140)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4632..4651)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(5311..6102,6103..6171))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5934..5953
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(6172..6276)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      join(6303..6488,1..270)
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6390..6409)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 tacggcacct cgaccccaaa aaacttgatt agggtgatgg ttcacgtagt gggccatcgc
       61 cctgatagac ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct
      121 tgttccaaac tggaacaaca ctcaacccta tctcggtcta ttcttttgat ttataaggga
      181 ttttgccgat ttcggcctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga
      241 attttaacaa aatattaacg cttacaattt ccattcgcca ttcaggctgc gcaactgttg
      301 ggaagggcga tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc
      361 tgcaaggcga ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac
      421 ggccagtgcc aagctgatct atacattgaa tcaatattgg caattagcca tattagtcat
      481 tggttatata gcataaatca atattggcta ttggccattg catacgttgt atctatatca
      541 taatatgtac atttatattg gctcatgtcc aatatgaccg ccatgttgac attgattatt
      601 gactagttat taatagtaat caattacggg gtcattagtt catagcccat atatggagtt
      661 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
      721 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
      781 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatgatat
      841 gccaagtccg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
      901 gtacatgacc ttacgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
      961 taccatggtg atgcggtttt ggcagtacac caatgggcgt ggatagcggt ttgactcacg
     1021 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     1081 acgggacttt ccaaaatgtc gtaataaccc cgccccgttg acgcaaatgg gcggtaggcg
     1141 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga attcagatct
     1201 ggtaccacgc gtatcgataa gcttgatcat ggcagaggag caaaagctca tttctgaaga
     1261 ggacttgttg cggaaacata tggcggaggt gggggagata atcgagggct gccgcctacc
     1321 cgtgctgcgg cggaaccagg acaacgaaga tgagtggccc ctggccgaga tcctgagcgt
     1381 gaaggacatc agtggccgga agcttttcta cgtccattac attgacttca acaaacgtct
     1441 ggatgaatgg gtgacgcatg agcggctgga cctaaagaag atccagttcc ccaagaaaga
     1501 ggccaagacc cccactaaga acggacttcc tgggtcccgt cctggctctc cagagagaga
     1561 ggtgccggcc tcggcgcagg ccagcgggaa gaccttgcca atcccggtcc agatcacact
     1621 ccgcttcaac ctgcccaagg agcgggaggc cattcccggt ggcgagcctg accagccgct
     1681 ctcctccagc tcctgcctgc agcccaacca ccgctcaacg aaacggaagg tggaggtggt
     1741 ttcaccagca actccagtgc ccagcgagac agccccggcc tcggtttttc cccagaatgg
     1801 agccgcccgt agggcagtgg cagcccagcc aggacggaag cgaaaatcga attgtttggg
     1861 cactgatgag gactcccagg acagctctga tggaataccg tcagcaccac gcatgactgg
     1921 cagcctggtg tctgatcgaa gccacgacga catcgtcacc cggatgaaga acattgagtg
     1981 cattgagctg ggccggcacc gcctcaagcc gtggtacttc tccccgtacc cacaggaact
     2041 caccacattg cctgtcctct acctgtgcga gttctgcctc aagtacggcc gtagtctcaa
     2101 gtgtcttcag cgtcatttga ccaagtgtga cctacgacat cctccaggca atgagattta
     2161 ccgcaagggc accatctcct tctttgagat tgatggacgt aagaacaaga gttattccca
     2221 gaacctgtgt cttttggcca agtgtttcct tgaccataag acactgtact atgacacaga
     2281 ccctttcctc ttctacgtca tgacagagta tgactgtaag ggcttccaca tcgtgggcta
     2341 cttctccaag gagaaagaat caacggaaga ctacaatgtg gcctgcatcc taaccctgcc
     2401 tccctaccag cgccggggct acggcaagct gctgatcgag ttcagctatg aactctccaa
     2461 agtggaaggg aaaacaggga cccctgagaa gcccctctca gaccttggcc tcctatccta
     2521 tcgaagctac tggtcccaga ccatcctgga gatcctgatg gggctgaagt cggagagcgg
     2581 ggagaggcca cagatcacca tcaatgagat tagtgaaatc accagcatca agaaggagga
     2641 tgtcatctcc actctgcagt acctcaatct catcaactac tacaagggcc agtacatcct
     2701 cacactgtca gaggacatcg tggatggcca tgagcgggcc atgctcaagc ggctcctgcg
     2761 gatcgactcc aagtgtctgc acttcactcc caaggactgg agcaagaggg ggaagtggtg
     2821 accagacact gcccactgca gtgccaagac ggcagcagga ctggggctga tagcccaccc
     2881 cgcccccact gcagctccca caaagcactc taagggagat ggggctgagg acagctcaaa
     2941 aaggagagga caggcctggc aggggcccac tggtgcccag caccaaggcg agctccgggc
     3001 tcagaccaac tccaaggtca gctggccaca ggcccaggcc tcctctgaag cagggaccag
     3061 agggagccag gcagctgtgt acagtgagaa gggatcccgg gtggcatccc tgtgacccct
     3121 ccccagtgcc tctcctggcc ctggaagttg ccactccagt gcccaccagc cttgtcctaa
     3181 taaaattaag ttgcatcatt ttgtctgact aggtgtcctt ctataatatt atggggtgga
     3241 ggggggtggt atggagcaag gggcaagttg ggaagacaac ctgtagggcc tgcggggtct
     3301 attgggaacc aagctggagt gcagtggcac aatcttggct cactgcaatc tccgcctcct
     3361 gggttcaagc gattctcctg cctcagcctc ccgagttgtt gggattccag gcatgcatga
     3421 ccaggctcag ctaatttttg tttttttggt agagacgggg tttcaccata ttggccaggc
     3481 tggtctccaa ctcctaatct caggtgatct acccaccttg gcctcccaaa ttgctgggat
     3541 tacaggcgtg aaccactgct cccttccctg tccttctgat tttaaaataa ctataccagc
     3601 aggaggacgt ccagacacag cataggctac ctggccatgc ccaaccggtg ggacatttga
     3661 gttgcttgct tggcactgtc ctctcatgcg ttgggtccac tcagtagatg cctgttgaat
     3721 tgggtacgcg gccagcttgg ctgtggaatg tgtgtcagtt agggtgtgga aagtccccag
     3781 gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca accaggtgtg
     3841 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     3901 caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc agttccgccc
     3961 attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag gccgcctcgg
     4021 cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc ttttgcaaaa
     4081 agctcctcga ggaactgaaa aaccagaaag ttaattccct atagtgagtc gtattaaatt
     4141 cgtaatcatg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     4201 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     4261 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     4321 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     4381 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     4441 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     4501 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     4561 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     4621 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     4681 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     4741 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     4801 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     4861 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     4921 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     4981 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     5041 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     5101 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     5161 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     5221 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     5281 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     5341 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     5401 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     5461 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     5521 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     5581 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     5641 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     5701 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     5761 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     5821 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     5881 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     5941 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     6001 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     6061 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     6121 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     6181 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     6241 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     6301 tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac
     6361 cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt cctttctcgc
     6421 cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag ggttccgatt
     6481 tagtgctt
//
