LOCUS       sequence-424336-        8942 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG epitope-tagged GeNL ""Green"" BRET reporter in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8942)
  AUTHORS   Labra B, Parag-Sharma K, Powers JJ, Srivastava S, Walker JR, 
            Kirkland TA, Brennan CK, Prescher JA, Amelio AL
  TITLE     Optimized in vivo multispectral bioluminescent imaging of tumor 
            biology using engineered BRET reporters.
  JOURNAL   iScience. 2024 Aug 8;27(9):110655. doi: 10.1016/j.isci.2024.110655. 
            eCollection 2024 Sep 20.
  PUBMED    39252965
REFERENCE   2  (bases 1 to 8942)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-08-8"; volume: "27(9):110655. doi"; pages: " 
            10.1016/j.isci.2024.110655. eCollection 2024 Sep 20"
COMMENT     Sequence Label: pLenti_CMV:Flag-GeNL
FEATURES             Location/Qualifiers
     source          1..8942
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(376..387)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             580..813
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    885..1006
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(922..941)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     976..995
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      1046..1181
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(1201..1220)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1202..1220)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1230..1247)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(1230..1246)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1239..1261)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      1388..1843
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(1475..1494)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     1685..1706
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        1869..1973
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(1974..2042,2043..2834)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(2192..2211)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      3005..3593
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3494..3513
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3747..3764
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    3881..3902
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(3917..3922,3923..3940,3941..3947)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3955..3971
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     3960..3982
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     3979..3995
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3979..3995
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     4014..4034
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        4016..4034
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        4062..4288
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             4289..4469
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    4516..4641
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5134..5367
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             5552..5596
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    5863..5979
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        6002..6305
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6306..6509
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     6460..6480
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     6506..6530
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             6589..6612
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     primer_bind     complement(7837..7857)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        7842..8171
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8022..8157
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             8181..8780
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(8181..8200)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     8677..8697
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    join(8847..8942,1..493)
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(8900..8920)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
ORIGIN
        1 ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta taaatcctgg
       61 ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt ggtgtgcact
      121 gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca gctcctttcc
      181 gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc ctgccttgcc
      241 cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt gtcggggaag
      301 ctgacgtcct ttccatggct gctcgcctgt gttgccacct ggattctgcg cgggacgtcc
      361 ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg cctgctgccg
      421 gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat ctccctttgg
      481 gccgcctccc cgcctggaat tcgagctcgg tacctttaag accaatgact tacaaggcag
      541 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc
      601 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag
      661 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt
      721 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca
      781 gaccctttta gtcagtgtgg aaaatctcta gcagtagtag ttcatgtcat cttattattc
      841 agtatttata acttgcaaag aaatgaatat cagagagtga gaggaacttg tttattgcag
      901 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt
      961 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctggctct
     1021 agctatcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt ccgcccattc
     1081 tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg cctcggcctc
     1141 tgagctattc cagaagtagt gaggaggctt ttttggaggc ctagggacgt acccaattcg
     1201 ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg tcgtgactgg
     1261 gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg
     1321 cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc
     1381 gaatgggacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     1441 gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     1501 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     1561 cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga tggttcacgt
     1621 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     1681 aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     1741 gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     1801 aaatttaacg cgaattttaa caaaatatta acgcttacaa tttaggtggc acttttcggg
     1861 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
     1921 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     1981 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     2041 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     2101 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     2161 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     2221 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     2281 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     2341 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     2401 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     2461 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     2521 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     2581 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     2641 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     2701 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     2761 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     2821 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
     2881 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
     2941 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
     3001 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
     3061 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
     3121 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
     3181 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
     3241 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
     3301 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
     3361 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
     3421 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
     3481 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
     3541 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
     3601 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc
     3661 ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg
     3721 ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc
     3781 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
     3841 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
     3901 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
     3961 gcggataaca atttcacaca ggaaacagct atgaccatga ttacgccaag cgcgcaatta
     4021 accctcacta aagggaacaa aagctggagc tgcaagctta atgtagtctt atgcaatact
     4081 cttgtagtct tgcaacatgg taacgatgag ttagcaacat gccttacaag gagagaaaaa
     4141 gcaccgtgca tgccgattgg tggaagtaag gtggtacgat cgtgccttat taggaaggca
     4201 acagacgggt ctgacatgga ttggacgaac cactgaattg ccgcattgca gagatattgt
     4261 atttaagtgc ctagctcgat acataaacgg gtctctctgg ttagaccaga tctgagcctg
     4321 ggagctctct ggctaactag ggaacccact gcttaagcct caataaagct tgccttgagt
     4381 gcttcaagta gtgtgtgccc gtctgttgtg tgactctggt aactagagat ccctcagacc
     4441 cttttagtca gtgtggaaaa tctctagcag tggcgcccga acagggactt gaaagcgaaa
     4501 gggaaaccag aggagctctc tcgacgcagg actcggcttg ctgaagcgcg cacggcaaga
     4561 ggcgaggggc ggcgactggt gagtacgcca aaaattttga ctagcggagg ctagaaggag
     4621 agagatgggt gcgagagcgt cagtattaag cgggggagaa ttagatcgcg atgggaaaaa
     4681 attcggttaa ggccaggggg aaagaaaaaa tataaattaa aacatatagt atgggcaagc
     4741 agggagctag aacgattcgc agttaatcct ggcctgttag aaacatcaga aggctgtaga
     4801 caaatactgg gacagctaca accatccctt cagacaggat cagaagaact tagatcatta
     4861 tataatacag tagcaaccct ctattgtgtg catcaaagga tagagataaa agacaccaag
     4921 gaagctttag acaagataga ggaagagcaa aacaaaagta agaccaccgc acagcaagcg
     4981 gccgctgatc ttcagacctg gaggaggaga tatgagggac aattggagaa gtgaattata
     5041 taaatataaa gtagtaaaaa ttgaaccatt aggagtagca cccaccaagg caaagagaag
     5101 agtggtgcag agagaaaaaa gagcagtggg aataggagct ttgttccttg ggttcttggg
     5161 agcagcagga agcactatgg gcgcagcgtc aatgacgctg acggtacagg ccagacaatt
     5221 attgtctggt atagtgcagc agcagaacaa tttgctgagg gctattgagg cgcaacagca
     5281 tctgttgcaa ctcacagtct ggggcatcaa gcagctccag gcaagaatcc tggctgtgga
     5341 aagataccta aaggatcaac agctcctggg gatttggggt tgctctggaa aactcatttg
     5401 caccactgct gtgccttgga atgctagttg gagtaataaa tctctggaac agatttggaa
     5461 tcacacgacc tggatggagt gggacagaga aattaacaat tacacaagct taatacactc
     5521 cttaattgaa gaatcgcaaa accagcaaga aaagaatgaa caagaattat tggaattaga
     5581 taaatgggca agtttgtgga attggtttaa cataacaaat tggctgtggt atataaaatt
     5641 attcataatg atagtaggag gcttggtagg tttaagaata gtttttgctg tactttctat
     5701 agtgaataga gttaggcagg gatattcacc attatcgttt cagacccacc tcccaacccc
     5761 gaggggaccc gacaggcccg aaggaataga agaagaaggt ggagagagag acagagacag
     5821 atccattcga ttagtgaacg gatctcgacg gtatcggtta acttttaaaa gaaaaggggg
     5881 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     5941 taaagaatta caaaaacaaa ttacaaaatt caaaatttta tcgataagct tgggagttcc
     6001 gcgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac ccccgcccat
     6061 tgacgtcaat aatgacgtat gttcccatag taacgccaat agggactttc cattgacgtc
     6121 aatgggtgga gtatttacgg taaactgccc acttggcagt acatcaagtg tatcatatgc
     6181 caagtacgcc ccctattgac gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt
     6241 acatgacctt atgggacttt cctacttggc agtacatcta cgtattagtc atcgctatta
     6301 ccatggtgat gcggttttgg cagtacatca atgggcgtgg atagcggttt gactcacggg
     6361 gatttccaag tctccacccc attgacgtca atgggagttt gttttggcac caaaatcaac
     6421 gggactttcc aaaatgtcgt aacaactccg ccccattgac gcaaatgggc ggtaggcgtg
     6481 tacggtggga ggtctatata agcagagctc gtttagtgaa ccgtcagatc gcctggagac
     6541 gccatccacg ctgttttgac ctccatagaa gacaccgact ctagaatgga ctacaaggac
     6601 gacgatgaca aggtgtccaa gggcgaagag gacaacatgg ccagcctgcc tgccacccac
     6661 gagctgcaca tcttcggcag catcaacggc gtggacttcg acatggtggg acagggcacc
     6721 ggcaacccca acgacggcta cgaggaactg aacctgaagt ccacaaaggg cgacctgcag
     6781 ttcagcccct ggattctggt gccccacatc ggctacggct tccaccagta cctgccctac
     6841 cccgacggca tgagcccttt ccaggccgct atggtggatg gcagcggcta ccaggtgcac
     6901 cggaccatgc agtttgagga cggcgccagc ctgaccgtga actaccggta cacatacgag
     6961 ggcagccaca tcaagggcga ggcccaagtg aagggcacag gctttccagc cgacggcccc
     7021 gtgatgacca atagcctgac agccgccgac tggtgcagaa gcaagaaaac ctaccccaat
     7081 gacaagacca tcatcagcac cttcaagtgg tcctacacca ccggcaatgg caagcggtac
     7141 agaagcaccg cccggaccac ctacaccttc gccaaaccta tggccgccaa ctacctgaag
     7201 aaccagccta tgtacgtgtt ccgcaagacc gagctgaagc actccaagac agaactgaac
     7261 ttcaaagagt ggcagaaagc cttcaccggg tttgaagatt tcgttgggga ctggcgacag
     7321 acagccggct acaacctgga ccaagtcctt gaacagggag gtgtgtccag tttgtttcag
     7381 aatctcgggg tgtccgtaac tccgatccaa aggattgtcc tgagcggtga aaatgggctg
     7441 aagatcgaca tccatgtcat catcccgtat gaaggtctga gcggcgacca aatgggccag
     7501 atcgaaaaaa tttttaaggt ggtgtaccct gtggatgatc atcactttaa ggtgatcctg
     7561 cactatggca cactggtaat cgacggggtt acgccgaaca tgatcgacta tttcggacgg
     7621 ccgtatgaag gcatcgccgt gttcgacggc aaaaagatca ctgtaacagg gaccctgtgg
     7681 aacggcaaca aaattatcga cgagcgcctg atcaaccccg acggctccct gctgttccga
     7741 gtaaccatca acggagtgac cggctggcgg ctgtgcgaac gcattctggc gtaaggatcc
     7801 ggactagtta acgcgtcgac cagtggtcga ccctgtggaa tgtgtgtcag ttagggtgtg
     7861 gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag
     7921 caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc
     7981 tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc
     8041 ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg
     8101 aggccgcctc ggcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag
     8161 gcttttgcaa aaagcttacc atgaccgagt acaagcccac ggtgcgcctc gccacccgcg
     8221 acgacgtccc cagggccgta cgcaccctcg ccgccgcgtt cgccgactac cccgccacgc
     8281 gccacaccgt cgatccggac cgccacatcg agcgggtcac cgagctgcaa gaactcttcc
     8341 tcacgcgcgt cgggctcgac atcggcaagg tgtgggtcgc ggacgacggc gccgcggtgg
     8401 cggtctggac cacgccggag agcgtcgaag cgggggcggt gttcgccgag atcggcccgc
     8461 gcatggccga gttgagcggt tcccggctgg ccgcgcagca acagatggaa ggcctcctgg
     8521 cgccgcaccg gcccaaggag cccgcgtggt tcctggccac cgtcggcgtc tcgcccgacc
     8581 accagggcaa gggtctgggc agcgccgtcg tgctccccgg agtggaggcg gccgagcgcg
     8641 ccggggtgcc cgccttcctg gagacctccg cgccccgcaa cctccccttc tacgagcggc
     8701 tcggcttcac cgtcaccgcc gacgtcgagg tgcccgaagg accgcgcacc tggtgcatga
     8761 cccgcaagcc cggtgcctga cgcccgcccc acgacccgca gcgcccgacc gaaaggagcg
     8821 cacgacccca tgcatcgcca gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat
     8881 tgactggtat tcttaactat gttgctcctt ttacgctatg tggatacgct gctttaatgc
     8941 ct
//
