LOCUS       sequence-424337-        8957 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses FLAG epitope-tagged CeNL ""Blue"" BRET reporter in 
            mammalian cells..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8957)
  AUTHORS   Labra B, Parag-Sharma K, Powers JJ, Srivastava S, Walker JR, 
            Kirkland TA, Brennan CK, Prescher JA, Amelio AL
  TITLE     Optimized in vivo multispectral bioluminescent imaging of tumor 
            biology using engineered BRET reporters.
  JOURNAL   iScience. 2024 Aug 8;27(9):110655. doi: 10.1016/j.isci.2024.110655. 
            eCollection 2024 Sep 20.
  PUBMED    39252965
REFERENCE   2  (bases 1 to 8957)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "iScience.";
            date: "2024-08-8"; volume: "27(9):110655. doi"; pages: " 
            10.1016/j.isci.2024.110655. eCollection 2024 Sep 20"
COMMENT     Sequence Label: pLenti_CMV:Flag-CeNL
FEATURES             Location/Qualifiers
     source          1..8957
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     209..226
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    343..364
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(379..384,385..402,403..409)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    417..433
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     422..444
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     441..457
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     441..457
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     476..496
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        478..496
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     promoter        524..750
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             751..931
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    978..1103
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1596..1829
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2014..2058
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2325..2441
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        2464..2767
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2768..2971
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     2922..2942
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     2968..2992
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             3051..3074
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             join(3075..3077,3078..3590)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYFSDNVYITADKQKNGIKA
                     NFKIRHNIE"
     primer_bind     complement(3117..3138)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3378..3397)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3725..3746
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(4314..4334)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        4319..4648
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4499..4634
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             4658..5257
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(4658..4677)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     5154..5174
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     misc_feature    5324..5912
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(5377..5397)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(5795..5806)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5999..6232
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6304..6425
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6341..6360)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6395..6414
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     rep_origin      6465..6600
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6620..6639)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(6621..6639)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(6649..6666)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(6649..6665)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(6658..6680)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      6807..7262
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(6894..6913)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     7104..7125
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     promoter        7288..7392
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(7393..7461,7462..8253)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(7611..7630)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      join(8424..8957,1..55)
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     8913..8932
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
       61 cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
      121 tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
      181 cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
      241 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
      301 caggtttccc gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac
      361 tcattaggca ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt
      421 gagcggataa caatttcaca caggaaacag ctatgaccat gattacgcca agcgcgcaat
      481 taaccctcac taaagggaac aaaagctgga gctgcaagct taatgtagtc ttatgcaata
      541 ctcttgtagt cttgcaacat ggtaacgatg agttagcaac atgccttaca aggagagaaa
      601 aagcaccgtg catgccgatt ggtggaagta aggtggtacg atcgtgcctt attaggaagg
      661 caacagacgg gtctgacatg gattggacga accactgaat tgccgcattg cagagatatt
      721 gtatttaagt gcctagctcg atacataaac gggtctctct ggttagacca gatctgagcc
      781 tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag cttgccttga
      841 gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag atccctcaga
      901 cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac ttgaaagcga
      961 aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg cgcacggcaa
     1021 gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga ggctagaagg
     1081 agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg cgatgggaaa
     1141 aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata gtatgggcaa
     1201 gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca gaaggctgta
     1261 gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa cttagatcat
     1321 tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata aaagacacca
     1381 aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc gcacagcaag
     1441 cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag aagtgaatta
     1501 tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa ggcaaagaga
     1561 agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct tgggttcttg
     1621 ggagcagcag gaagcactat gggcgcagcg tcaatgacgc tgacggtaca ggccagacaa
     1681 ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga ggcgcaacag
     1741 catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat cctggctgtg
     1801 gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg aaaactcatt
     1861 tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga acagatttgg
     1921 aatcacacga cctggatgga gtgggacaga gaaattaaca attacacaag cttaatacac
     1981 tccttaattg aagaatcgca aaaccagcaa gaaaagaatg aacaagaatt attggaatta
     2041 gataaatggg caagtttgtg gaattggttt aacataacaa attggctgtg gtatataaaa
     2101 ttattcataa tgatagtagg aggcttggta ggtttaagaa tagtttttgc tgtactttct
     2161 atagtgaata gagttaggca gggatattca ccattatcgt ttcagaccca cctcccaacc
     2221 ccgaggggac ccgacaggcc cgaaggaata gaagaagaag gtggagagag agacagagac
     2281 agatccattc gattagtgaa cggatctcga cggtatcggt taacttttaa aagaaaaggg
     2341 gggattgggg ggtacagtgc aggggaaaga atagtagaca taatagcaac agacatacaa
     2401 actaaagaat tacaaaaaca aattacaaaa ttcaaaattt tatcgataag cttgggagtt
     2461 ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg acccccgccc
     2521 attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt tccattgacg
     2581 tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat
     2641 gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc attatgccca
     2701 gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat
     2761 taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt ttgactcacg
     2821 gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc accaaaatca
     2881 acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg gcggtaggcg
     2941 tgtacggtgg gaggtctata taagcagagc tcgtttagtg aaccgtcaga tcgcctggag
     3001 acgccatcca cgctgttttg acctccatag aagacaccga ctctagaatg gactacaagg
     3061 acgacgatga caaggtgagc aagggcgagg agctgttcac cggggtggtg cccatcctgg
     3121 tcgagctgga cggcgacgta aacggccaca agttcagcgt gtccggcgag ggcgagggcg
     3181 atgccaccta cggcaagctg accctgaagt tcatctgcac caccggcaag ctgcccgtgc
     3241 cctggcccac cctcgtgacc accctgtcct ggggcgtgca gtgcttcgcc cgctaccccg
     3301 accacatgaa gcagcacgac ttcttcaagt ccgccatgcc cgaaggctac gtccaggagc
     3361 gcaccatctt cttcaaggac gacggcaact acaagacccg cgccgaggtg aagttcgagg
     3421 gcgacaccct ggtgaaccgc atcgagctga agggcatcga cttcaaggag gacggcaaca
     3481 tcctggggca caagctggag tacaactact ttagcgacaa cgtctatatc accgccgaca
     3541 agcagaagaa cggcatcaag gccaacttca agatccgcca caacatcgag gacggcggcg
     3601 tgcagctcgc cgaccactac cagcagaaca cccccatcgg cgacggcccc gtgctgctgc
     3661 ccgacaacca ctacctgagc acccagtcca agctgagcaa agaccccaac gagaagcgcg
     3721 atcacatggt cctgctggag ttcgtgaccg ccgccgggtt gcatacactc gaagatttcg
     3781 ttggggactg gcgacagaca gccggctaca acctggacca agtccttgaa cagggaggtg
     3841 tgtccagttt gtttcagaat ctcggggtgt ccgtaactcc gatccaaagg attgtcctga
     3901 gcggtgaaaa tgggctgaag atcgacatcc atgtcatcat cccgtatgaa ggtctgagcg
     3961 gcgaccaaat gggccagatc gaaaaaattt ttaaggtggt gtaccctgtg gatgatcatc
     4021 actttaaggt gatcctgcac tatggcacac tggtaatcga cggggttacg ccgaacatga
     4081 tcgactattt cggacggccg tatgaaggca tcgccgtgtt cgacggcaaa aagatcactg
     4141 taacagggac cctgtggaac ggcaacaaaa ttatcgacga gcgcctgatc aaccccgacg
     4201 gctccctgct gttccgagta accatcaacg gagtgaccgg ctggcggctg tgcgaacgca
     4261 ttctggcgta aggatccgga ctagttaacg cgtcgaccag tggtcgaccc tgtggaatgt
     4321 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
     4381 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
     4441 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
     4501 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
     4561 tatttatgca gaggccgagg ccgcctcggc ctctgagcta ttccagaagt agtgaggagg
     4621 cttttttgga ggcctaggct tttgcaaaaa gcttaccatg accgagtaca agcccacggt
     4681 gcgcctcgcc acccgcgacg acgtccccag ggccgtacgc accctcgccg ccgcgttcgc
     4741 cgactacccc gccacgcgcc acaccgtcga tccggaccgc cacatcgagc gggtcaccga
     4801 gctgcaagaa ctcttcctca cgcgcgtcgg gctcgacatc ggcaaggtgt gggtcgcgga
     4861 cgacggcgcc gcggtggcgg tctggaccac gccggagagc gtcgaagcgg gggcggtgtt
     4921 cgccgagatc ggcccgcgca tggccgagtt gagcggttcc cggctggccg cgcagcaaca
     4981 gatggaaggc ctcctggcgc cgcaccggcc caaggagccc gcgtggttcc tggccaccgt
     5041 cggcgtctcg cccgaccacc agggcaaggg tctgggcagc gccgtcgtgc tccccggagt
     5101 ggaggcggcc gagcgcgccg gggtgcccgc cttcctggag acctccgcgc cccgcaacct
     5161 ccccttctac gagcggctcg gcttcaccgt caccgccgac gtcgaggtgc ccgaaggacc
     5221 gcgcacctgg tgcatgaccc gcaagcccgg tgcctgacgc ccgccccacg acccgcagcg
     5281 cccgaccgaa aggagcgcac gaccccatgc atcgccagtc gacaatcaac ctctggatta
     5341 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
     5401 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
     5461 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
     5521 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
     5581 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
     5641 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
     5701 cgtggtgttg tcggggaagc tgacgtcctt tccatggctg ctcgcctgtg ttgccacctg
     5761 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
     5821 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
     5881 gagtcggatc tccctttggg ccgcctcccc gcctggaatt cgagctcggt acctttaaga
     5941 ccaatgactt acaaggcagc tgtagatctt agccactttt taaaagaaaa ggggggactg
     6001 gaagggctaa ttcactccca acgaagacaa gatctgcttt ttgcttgtac tgggtctctc
     6061 tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc actgcttaag
     6121 cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt gtgtgactct
     6181 ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag cagtagtagt
     6241 tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc agagagtgag
     6301 aggaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     6361 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     6421 tcttatcatg tctggctcta gctatcccgc ccctaactcc gcccatcccg cccctaactc
     6481 cgcccagttc cgcccattct ccgccccatg gctgactaat tttttttatt tatgcagagg
     6541 ccgaggccgc ctcggcctct gagctattcc agaagtagtg aggaggcttt tttggaggcc
     6601 tagggacgta cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt
     6661 tttacaacgt cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca
     6721 tccccctttc gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca
     6781 gttgcgcagc ctgaatggcg aatgggacgc gccctgtagc ggcgcattaa gcgcggcggg
     6841 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
     6901 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     6961 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     7021 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     7081 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
     7141 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
     7201 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgcttacaat
     7261 ttaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata
     7321 cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga
     7381 aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca
     7441 ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat
     7501 cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag
     7561 agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc
     7621 gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat acactattct
     7681 cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga tggcatgaca
     7741 gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc caacttactt
     7801 ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat gggggatcat
     7861 gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa cgacgagcgt
     7921 gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac tggcgaacta
     7981 cttactctag cttcccggca acaattaata gactggatgg aggcggataa agttgcagga
     8041 ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc tggagccggt
     8101 gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc ctcccgtatc
     8161 gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag acagatcgct
     8221 gagataggtg cctcactgat taagcattgg taactgtcag accaagttta ctcatatata
     8281 ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     8341 gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     8401 gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     8461 caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     8521 ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt tcttctagtg
     8581 tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     8641 ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     8701 tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     8761 cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     8821 gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     8881 ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     8941 gtcgggtttc gccacct
//
