LOCUS       sequence-424345-        3220 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Helper plasmid for pORI plasmids. Chloramphenicol resistant, 
            temperature sensitive replication in E. coli and gram positives. 
            Encodes RepA..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 3220)
  AUTHORS   Russell WM, Klaenhammer TR
  TITLE     Efficient system for directed integration into the Lactobacillus 
            acidophilus and Lactobacillus gasseri chromosomes via homologous 
            recombination.
  JOURNAL   Appl Environ Microbiol. 2001 Sep;67(9):4361-4.
  PUBMED    11526048
REFERENCE   2  (bases 1 to 3220)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Appl 
            Environ Microbiol."; date: "2001-09"; volume: "67(9)"; pages: 
            "4361-4"
COMMENT     Sequence Label: pTRK669
FEATURES             Location/Qualifiers
     source          1..3220
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(62..73)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
ORIGIN
        1 gcagcgaaga tgttgtctgt tagattatga aagccgatga ctgaatgaaa taataagcgc
       61 agcgcccttc tatttcggtt ggaggaggct caagggagta tgagggaatg aaattccctc
      121 atgggtttga ttttaaaaat tgcttgcaat tttgccgagc ggtagcgctg gaaaattttt
      181 gaaaaaaatt tggaatttgg aaaaaaatgg ggggaaagga agcgaatttt gcttccgtac
      241 tacgaccccc cattaagtgc cgagtgccaa tttttgtgcc aaaaacgctc tatcccaact
      301 ggctcaaggg tttaaggggt ttttcaatcg ccaacgaatc gccaacgttt tcgccaacgt
      361 tttttataaa tctatattta agtagcttta ttgttgtttt tatgattaca aagtgataca
      421 ctaactttat aaaattattt gattggagtt ttttaaatgg tgatttcaga atcgaaaaaa
      481 agagttatga tttctctgac aaaagagcaa gataaaaaat taacagatat ggcgaaacaa
      541 aaaggttttt caaaatctgc ggttgcggcg ttagctatag aagaatatgc aagaaaggaa
      601 tcagaacaaa aaaaataagc gaaagctcgc gtttttagaa ggatacgagt tttcgctact
      661 tgtttttgat aaggtaatta tatcatggct attaaaaata ctaaagctag aaattttgga
      721 tttttattat atcctgactc aattcctaat gattggaaag aaaaattaga gagtttgggc
      781 gtatctatgg ctgtcagtcc tttacacgat atggacgaaa aaaaagataa agatacatgg
      841 aatagtagtg atgttatacg aaatggaaag cactataaaa aaccacacta tcacgttata
      901 tatattgcac gaaatcctgt aacaatagaa agcgttagga acaagattaa gcgaaaattg
      961 gggaatagtt cagttgctca tgttgagata cttgattata tcaaaggttc atatgaatat
     1021 ttgactcatg aatcaaagga cgctattgct aagaataaac atatatacga caaaaaagat
     1081 attttgaaca ttaatgattt tgatattgac cgctatataa cacttgatga aagccaaaaa
     1141 agagaattga agaatttact tttagatata gtggatgact ataatttggt aaatacaaaa
     1201 gatttaatgg cttttattcg ccttagggga gcggagtttg gaattttaaa tacgaatgat
     1261 gtaaaagata ttgtttcaac aaactctagc gcctttagat tatggtttga gggcaattat
     1321 cagtgtggat atagagcaag ttatgcaaag gttcttgatg ctgaaacggg ggaaataaaa
     1381 tgacaaacaa agaaaaagag ttatttgctg aaaatgagga attaaaaaaa gaaattaagg
     1441 acttaaaaga gcgtattgaa agatacagag aaatggaagt tgaattaagt acaacaatag
     1501 atttattgag aggagggatt attgaataaa taaaagcccc ctgacgaaag tcgaaggggg
     1561 tttttatttt ggtttgatgt tgcgattaat agcaatacaa ttgcaataaa caaaatgatc
     1621 ttccttcagg ttatgaccat ctgtgccagt tcgtaatgtc tggtcaactt tccgactctg
     1681 agaaacttct ggaatcgcta gagaatttct ggaatgggat tcaggagtgg acagaacgac
     1741 acggatatat agtggatgtg tcaaaacgca taccattttg aacgatgacc tctaataatt
     1801 gttaatcatg ttggttacgt atttattaac ttctcctagt attagtaatt atcatggctg
     1861 tcatggcgca ttaacggaat aaagggtgtg cttaaatcgg gccattttgc gtaataagaa
     1921 aaaggattaa ttatgagcga attgaattaa taataaggta atagatttac attagaaaat
     1981 gaaaggggat tttatgcgtg agaatgttac agtctatccc ggcaatagtt acccttatta
     2041 tcaagataag aaagaaaagg atttttcgct acgctcaaat cctttaaaaa aacacaaaag
     2101 accacatttt ttaatgtggt cttttattct tcaactaaag cacccattag ttcaacaaac
     2161 gaaaattgga taaagtggga tatttttaaa atatatattt atgttacagt aatattgact
     2221 tttaaaaaag gattgattct aatgaagaaa gcagacaagt aagcctccta aattcacttt
     2281 agataaaaat ttaggaggca tatcaaatga actttaataa aattgattta gacaattgga
     2341 agagaaaaga gatatttaat cattatttga accaacaaac gacttttagt ataaccacag
     2401 aaattgatat tagtgtttta taccgaaaca taaaacaaga aggatataaa ttttaccctg
     2461 catttatttt cttagtgaca agggtgataa actcaaatac agcttttaga actggttaca
     2521 atagcgacgg agagttaggt tattgggata agttagagcc actttataca atttttgatg
     2581 gtgtatctaa aacattctct ggtatttgga ctcctgtaaa gaatgacttc aaagagtttt
     2641 atgatttata cctttctgat gtagagaaat ataatggttc ggggaaattg tttcccaaaa
     2701 cacctatacc tgaaaatgct ttttctcttt ctattattcc atggacttca tttactgggt
     2761 ttaacttaaa tatcaataat aatagtaatt accttctacc cattattaca gcaggaaaat
     2821 tcattaataa aggtaattca atatatttac cgctatcttt acaggtacat cattctgttt
     2881 gtgatggtta tcatgcagga ttgtttatga actctattca ggaattgtca gataggccta
     2941 atgactggct tttataatat gagataatgc cgactgtact ttttacagtc ggttttctaa
     3001 tgtcactaga tctagtctac ttaattacga agtaaataag tctagtgtgt tagactttat
     3061 gaaatctata tacgtttata tatatttatt atccgatttt ttattaaaac gtctcaaaat
     3121 cgtttctgag acgttttagc gtttatttcg tttagttatc ggcataatcg ttaaaacagg
     3181 cgttatcgta gcgtaaaagc cttgagcgta gcgtggcttt
//
