LOCUS       sequence-424347-        5607 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Truncated rat TH promoter expressing iCre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5607)
  AUTHORS   Lycas MD, Ejdrup AL, Sorensen AT, Haahr NO, Jorgensen SH, Guthrie 
            DA, Stoier JF, Werner C, Newman AH, Sauer M, Herborg F, Gether U
  TITLE     Nanoscopic dopamine transporter distribution and conformation are 
            inversely regulated by excitatory drive and D2 autoreceptor 
            activity.
  JOURNAL   Cell Rep. 2022 Sep 27;40(13):111431. doi: 
            10.1016/j.celrep.2022.111431.
  PUBMED    36170827
REFERENCE   2  (bases 1 to 5607)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Rep.";
            date: "2022-09-27"; volume: "40(13):111431. doi"; pages: " 
            10.1016/j.celrep.2022.111431"
COMMENT     Sequence Label: pAAV-pTH-iCre-WPREpA
FEATURES             Location/Qualifiers
     source          1..5607
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   19..148
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     CDS             678..698
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    1752..2340
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1805..1825)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2223..2234)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    2372..2848
                     /label=hGH poly(A) signal
                     /note="human growth hormone polyadenylation signal"
     primer_bind     complement(2571..2590)
                     /label=hGH-PA-R
                     /note="Human growth hormone terminator, reverse primer"
     repeat_region   2888..3028
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   2888..3017
                     /label=AAV2 ITR
     rep_origin      3103..3558
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3190..3209)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3400..3421
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3575..3594)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     3694..3716
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(3754..3772)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        3840..3944
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3945..4013,4014..4805)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4163..4182)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4976..5564
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5465..5484
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 ggccttttgc tcacatgtcc tgcaggcagc tgcgcgctcg ctcgctcact gaggccgccc
       61 gggcgtcggg cgacctttgg tcgcccggcc tcagtgagcg agcgagcgcg cagagaggga
      121 gtggccaact ccatcactag gggttcctgc ggccgcacgc gtatagatag tacactttgt
      181 tacatgggct ggggggaaca tggcccatgt cctggagggg actttatgac agacatccaa
      241 aaatccagtg agagggcttc tagatttgtc tccaaaggtt atagttctaa catgagccct
      301 taggaaatcc agcatggttc tccctgtgtg ccctggtttg gttagagagc tctagcggtc
      361 tcctgtccca cagaatacca gccagcccct gccctacgtc gtgcctcggg ctgagggtga
      421 ttcagaggca ggtgcctgtg acagtggatg caattagatc taatgggacg gaggcctttc
      481 tcgtcgccct cgctccatgc ccacccccgc ctccctcagg cacagcaggc gtggagagga
      541 tgcgcaggag gtaggaggtg ggggacccag aggggctttg acgtcagcct ggcctttaaa
      601 gagggcgcct gcctggcgag ggctgtggag acagaactcg ggaccaccag ggatccttcg
      661 aaaccggtac catggtgccc aagaagaaga ggaaagtctc caacctgctg actgtgcacc
      721 aaaacctgcc tgccctccct gtggatgcca cctctgatga agtcaggaag aacctgatgg
      781 acatgttcag ggacaggcag gccttctctg aacacacctg gaagatgctc ctgtctgtgt
      841 gcagatcctg ggctgcctgg tgcaagctga acaacaggaa atggttccct gctgaacctg
      901 aggatgtgag ggactacctc ctgtacctgc aagccagagg cctggctgtg aagaccatcc
      961 aacagcacct gggccagctc aacatgctgc acaggagatc tggcctgcct cgcccttctg
     1021 actccaatgc tgtgtccctg gtgatgagga gaatcagaaa ggagaatgtg gatgctgggg
     1081 agagagccaa gcaggccctg gcctttgaac gcactgactt tgaccaagtc agatccctga
     1141 tggagaactc tgacagatgc caggacatca ggaacctggc cttcctgggc attgcctaca
     1201 acaccctgct gcgcattgcc gaaattgcca gaatcagagt gaaggacatc tcccgcaccg
     1261 atggtgggag aatgctgatc cacattggca ggaccaagac cctggtgtcc acagctggtg
     1321 tggagaaggc cctgtccctg ggggttacca agctggtgga gagatggatc tctgtgtctg
     1381 gtgtggctga tgaccccaac aactacctgt tctgccgggt cagaaagaat ggtgtggctg
     1441 ccccttctgc cacctcccaa ctgtccaccc gggccctgga agggatcttt gaggccaccc
     1501 accgcctgat ctatggtgcc aaggatgact ctgggcagag atacctggcc tggtctggcc
     1561 actctgccag agtgggtgct gccagggaca tggccagggc tggtgtgtcc atccctgaaa
     1621 tcatgcaggc tggtggctgg accaatgtga acattgtgat gaactacatc agaaacctgg
     1681 actctgagac tggggccatg gtgaggctgc tcgaggatgg ggactaagaa ttcgatatca
     1741 agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact ggtattctta
     1801 actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg tatcatgcta
     1861 ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg ctgtctcttt
     1921 atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg
     1981 caacccccac tggttggggc attgccacca cctgtcagct cctttccggg actttcgctt
     2041 tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc tgctggacag
     2101 gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca tcgtcctttc
     2161 cttggctgct cgcctatgtt gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc
     2221 cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct ctgcggcctc
     2281 ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc gcctccccgc
     2341 atcgataccg agcgctgctc gagagatcta cgggtggcat ccctgtgacc cctccccagt
     2401 gcctctcctg gccctggaag ttgccactcc agtgcccacc agccttgtcc taataaaatt
     2461 aagttgcatc attttgtctg actaggtgtc cttctataat attatggggt ggaggggggt
     2521 ggtatggagc aaggggcaag ttgggaagac aacctgtagg gcctgcgggg tctattggga
     2581 accaagctgg agtgcagtgg cacaatcttg gctcactgca atctccgcct cctgggttca
     2641 agcgattctc ctgcctcagc ctcccgagtt gttgggattc caggcatgca tgaccaggct
     2701 cagctaattt ttgttttttt ggtagagacg gggtttcacc atattggcca ggctggtctc
     2761 caactcctaa tctcaggtga tctacccacc ttggcctccc aaattgctgg gattacaggc
     2821 gtgaaccact gctcccttcc ctgtccttct gattttgtag gtaaccacgt gcggaccgag
     2881 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     2941 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     3001 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     3061 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     3121 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
     3181 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     3241 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     3301 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     3361 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     3421 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     3481 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     3541 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     3601 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     3661 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     3721 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     3781 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     3841 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     3901 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     3961 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     4021 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     4081 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     4141 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     4201 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     4261 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     4321 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     4381 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     4441 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     4501 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     4561 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     4621 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     4681 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     4741 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     4801 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     4861 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     4921 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     4981 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     5041 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     5101 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     5161 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     5221 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     5281 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     5341 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     5401 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     5461 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     5521 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     5581 cctttttacg gttcctggcc ttttgct
//
