LOCUS       sequence-424349-       12378 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  CIRTS RNA targeting system for targeted knockdown in neurons. 
            CIRTS-Calm3 and scramble control guide RNA is expressed under human 
            Syn1 and U6 promoter, respectively..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 12378)
  AUTHORS   Liau WS, Zhao Q, Bademosi A, Gormal RS, Gong H, Marshall PR, 
            Periyakaruppiah A, Madugalle SU, Zajaczkowski EL, Leighton LJ, Ren 
            H, Musgrove M, Davies J, Rauch S, He C, Dickinson BC, Li X, Wei W, 
            Meunier FA, Fernandez-Moya SM, Kiebler MA, Srinivasan B, Banerjee S,
            Clark M, Spitale RC, Bredy TW
  TITLE     Fear extinction is regulated by the activity of long noncoding RNAs 
            at the synapse.
  JOURNAL   Nat Commun. 2023 Nov 22;14(1):7616. doi: 10.1038/s41467-023-43535-1.
  PUBMED    37993455
REFERENCE   2  (bases 1 to 12378)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2023-11-22"; volume: "14(1):7616. doi"; pages: " 
            10.1038/s41467-023-43535-1"
COMMENT     Sequence Label: CIRTS-control
FEATURES             Location/Qualifiers
     source          1..12378
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(79..98)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        270..649
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        651..849
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     804..824
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     LTR             867..1047
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    1094..1219
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1712..1945
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             2130..2174
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    2472..2589
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     regulatory      3758..3767
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             join(3764..3766,3767..3769,3770..4480)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3764..3766,3767..3769,3770..4480)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3809..3830)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(4070..4089)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     4417..4438
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             4490..4543
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             5060..5086
                     /codon_start=1
                     /product="nuclear export signal from the HIV Rev protein 
                     (Fischer et al., 1995)"
                     /label=NES
                     /translation="LPPLERLTL"
     CDS             5690..5713
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        6747..6995
                     /label=U6 promoter
                     /note="RNA polymerase III promoter for human U6 snRNA 
                     (Domitrovich & Kunkel, 2003)"
     primer_bind     6747..6767
                     /label=hU6-F
                     /note="Human U6 promoter, forward primer"
     primer_bind     6918..6937
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_feature    7102..7690
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(7155..7175)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(7573..7584)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(7693..7709)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7694..7710)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             8215..8395
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(8421..8438)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    8427..8651
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      8697..9125
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(8784..8803)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     8994..9015
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(9134..9154)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        9139..9468
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      9319..9454
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     9381..9400
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        9516..9563
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             9582..9956
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    10086..10207
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(10123..10142)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     10177..10196
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(10256..10272)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(10256..10272)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(10269..10291)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    10280..10296
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(10304..10310,10311..10328,10329..10334))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    10349..10370
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(10487..10504)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(10658..11246)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(10738..10757)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(11417..12208,12209..12277))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     12040..12059
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(join(12278..12378,1..4))
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 cgcgcacatt tccccgaaaa gtgccacctg acgtcgacgg atcgggagat ctcccgatcc
       61 cctatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agtatctgct
      121 ccctgcttgt gtgttggagg tcgctgagta gtgcgcgagc aaaatttaag ctacaacaag
      181 gcaaggcttg accgacaatt gcatgaagaa tctgcttagg gttaggcgtt ttgcgctgct
      241 tcgcgatgta cgggccagat atacgcgttg acattgatta ttgactagtt attaatagta
      301 atcaattacg gggtcattag ttcatagccc atatatggag ttccgcgtta cataacttac
      361 ggtaaatggc ccgcctggct gaccgcccaa cgacccccgc ccattgacgt caataatgac
      421 gtatgttccc atagtaacgc caatagggac tttccattga cgtcaatggg tggagtattt
      481 acggtaaact gcccacttgg cagtacatca agtgtatcat atgccaagta cgccccctat
      541 tgacgtcaat gacggtaaat ggcccgcctg gcattatgcc cagtacatga ccttatggga
      601 ctttcctact tggcagtaca tctacgtatt agtcatcgct attaccatgg tgatgcggtt
      661 ttggcagtac atcaatgggc gtggatagcg gtttgactca cggggatttc caagtctcca
      721 ccccattgac gtcaatggga gtttgttttg gcaccaaaat caacgggact ttccaaaatg
      781 tcgtaacaac tccgccccat tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta
      841 tataagcagc gcgttttgcc tgtactgggt ctctctggtt agaccagatc tgagcctggg
      901 agctctctgg ctaactaggg aacccactgc ttaagcctca ataaagcttg ccttgagtgc
      961 ttcaagtagt gtgtgcccgt ctgttgtgtg actctggtaa ctagagatcc ctcagaccct
     1021 tttagtcagt gtggaaaatc tctagcagtg gcgcccgaac agggacttga aagcgaaagg
     1081 gaaaccagag gagctctctc gacgcaggac tcggcttgct gaagcgcgca cggcaagagg
     1141 cgaggggcgg cgactggtga gtacgccaaa aattttgact agcggaggct agaaggagag
     1201 agatgggtgc gagagcgtca gtattaagcg ggggagaatt agatcgcgat gggaaaaaat
     1261 tcggttaagg ccagggggaa agaaaaaata taaattaaaa catatagtat gggcaagcag
     1321 ggagctagaa cgattcgcag ttaatcctgg cctgttagaa acatcagaag gctgtagaca
     1381 aatactggga cagctacaac catcccttca gacaggatca gaagaactta gatcattata
     1441 taatacagta gcaaccctct attgtgtgca tcaaaggata gagataaaag acaccaagga
     1501 agctttagac aagatagagg aagagcaaaa caaaagtaag accaccgcac agcaagcggc
     1561 cgctgatctt cagacctgga ggaggagata tgagggacaa ttggagaagt gaattatata
     1621 aatataaagt agtaaaaatt gaaccattag gagtagcacc caccaaggca aagagaagag
     1681 tggtgcagag agaaaaaaga gcagtgggaa taggagcttt gttccttggg ttcttgggag
     1741 cagcaggaag cactatgggc gcagcgtcaa tgacgctgac ggtacaggcc agacaattat
     1801 tgtctggtat agtgcagcag cagaacaatt tgctgagggc tattgaggcg caacagcatc
     1861 tgttgcaact cacagtctgg ggcatcaagc agctccaggc aagaatcctg gctgtggaaa
     1921 gatacctaaa ggatcaacag ctcctgggga tttggggttg ctctggaaaa ctcatttgca
     1981 ccactgctgt gccttggaat gctagttgga gtaataaatc tctggaacag atttggaatc
     2041 acacgacctg gatggagtgg gacagagaaa ttaacaatta cacaagctta atacactcct
     2101 taattgaaga atcgcaaaac cagcaagaaa agaatgaaca agaattattg gaattagata
     2161 aatgggcaag tttgtggaat tggtttaaca taacaaattg gctgtggtat ataaaattat
     2221 tcataatgat agtaggaggc ttggtaggtt taagaatagt ttttgctgta ctttctatag
     2281 tgaatagagt taggcaggga tattcaccat tatcgtttca gacccacctc ccaaccccga
     2341 ggggacccga caggcccgaa ggaatagaag aagaaggtgg agagagagac agagacagat
     2401 ccattcgatt agtgaacgga tcggcactgc gtgcgccaat tctgcagaca aatggcagta
     2461 ttcatccaca attttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     2521 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     2581 caaaattttc gggtttatta cagggacagc agagatccag tttggttaat taagggtttt
     2641 ggctacgtcc agagcagagg aatgagggca tgtagactaa atatgttcgt gtggaagagg
     2701 ctgaatacac atcagagtta gtgctgcagg aaatgcttct gcattgcata cccagagttt
     2761 ccttgctcat ctgagagcat gtgttttttc cagatgtgtg tacttgtgtg agattctctg
     2821 ggtgtgtgtc aatgtgttgc ctgaacgtgc attgctcaat atgctcatgt gtgttaccct
     2881 gggcttgtac atctacatat atacctggat gcccgtgtgt tctgtgatgt acatataccc
     2941 tgtgtcattc cttgtttttc tatttgtgtt attccatgtg ttccttcagg ctctcactac
     3001 ccaagtgtcc acctccgcct gtctggtgat gtttacgcta ccccgtgctc ttttctttgc
     3061 ctgacagtgt tgtcgtggaa gacatctcgc caggaacact gcagtaagga gaatttctag
     3121 ttttatgttc ccctccgagt atgcttctat cccgaccctc aaccccaaaa tgccttcaga
     3181 ggtgaaaatc aacactggaa acacaagtat ctgggaaggg taacaatgca agttagcctg
     3241 aggatttagg aggaggctga aaaacagagt aggagcctta ctacgggtcc agaccctacg
     3301 gacaagaacc cccactccca ctccccaaat tgcgcattcc ctcccccatc agagggggag
     3361 gggaagagga tgcagcgcgg cgcggcgcgt gcgcactgtc ggatttagta ccgcggacag
     3421 agccttcgcc cccgctgccg gcgcgcgcca ccacctcccc agcaccaaag gcgggctgac
     3481 gtcactctcc agccctcccc aaactcccct acctcaccgc cttggtcgcg tccgtgcagc
     3541 ggtgagtcca gtcgggccgc accacaagag gtgcaagata ggggggtgca ggcgcgacca
     3601 tacgctctgc ggcggcagag cctcagcgct gcctcagtct gcagcgggca gcagaggagt
     3661 cgcgtcgtgc cagagagcgc cgccgtgctc ctgagcccct tgcgctccgc ccccgcggcc
     3721 caccgaccca ctgccccttg gatccccggg taccggtgcc accatggtga gcaagggcga
     3781 ggagctgttc accggggtgg tgcccatcct ggtcgagctg gacggcgacg taaacggcca
     3841 caagttcagc gtgtccggcg agggcgaggg cgatgccacc tacggcaagc tgaccctgaa
     3901 gttcatctgc accaccggca agctgcccgt gccctggccc accctcgtga ccaccctgac
     3961 ctacggcgtg cagtgcttca gccgctaccc cgaccacatg aagcagcacg acttcttcaa
     4021 gtccgccatg cccgaaggct acgtccagga gcgcaccatc ttcttcaagg acgacggcaa
     4081 ctacaagacc cgcgccgagg tgaagttcga gggcgacacc ctggtgaacc gcatcgagct
     4141 gaagggcatc gacttcaagg aggacggcaa catcctgggg cacaagctgg agtacaacta
     4201 caacagccac aacgtctata tcatggccga caagcagaag aacggcatca aggtgaactt
     4261 caagatccgc cacaacatcg aggacggcag cgtgcagctc gccgaccact accagcagaa
     4321 cacccccatc ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcacccagtc
     4381 cgccctgagc aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac
     4441 cgccgccggg atcactctcg gcatggacga gctgtacaag ggctccggcg agggcagggg
     4501 aagtctacta acatgcgggg acgtggagga aaatcccggc ccaaccggta tgggaattat
     4561 caacaccctt caaaaatact actgtcgcgt ccgtgggggt cgttgcgcag tcctgtcgtg
     4621 cctgcctaaa gaggagcaga tcggtaaatg ctcaacccgt ggacgtaagt gctgtcgtcg
     4681 caagaaaggc ggttctggag gctcaggggg gagcggaggg tccggtggat caggaggctc
     4741 catggcagtt cccgagaccc gccctaacca cactatttat atcaacaacc tcaatagcaa
     4801 gatcaagaag gatgagctaa aaaagtccct gtacgccatc ttctcccagt ttggccagat
     4861 cctggatatc ctggtaccgc ggcagcgtac cccgaggggc caggcctttg tcatcttcaa
     4921 ggaggtcagc agcgccacca acgccctgcg ctccatgcag ggtttccctt tctatgacaa
     4981 acctatgcgt atccagtatg ccaagaccga caaacgtatc ccggccaaga tgaaaggcac
     5041 cttcgtggga tcccttcaac tgcctccact tgaaagactg acactgggcg gtagtggagg
     5101 aagcgggggg agcggaggga gtggtggatc cggcggctcc atggaattgg agatccgtcc
     5161 gcttttcctg gttcccgata ccaatgggtt cattgaccac ttagcgagct tagcccgtct
     5221 gcttgagagc cgtaagtaca ttctggtggt tcctcttatt gtcatcaacg agttagacgg
     5281 ccttgcgaaa ggccaggaaa cagaccatcg tgcgggaggc tatgcgcgtg tcgtgcaaga
     5341 aaaggctcgc aagagcattg agtttttgga gcaacgtttc gagtcgcgtg actcctgttt
     5401 acgtgcactt accagccgtg gaaatgaact tgagtcaatc gcatttcgct ctgaagatat
     5461 cactgggcag ttaggtaaca atgacgattt gattttgtca tgctgcctgc actattgtaa
     5521 agacaaggca aaggacttta tgcccgcgtc gaaggaagag ccgatccgtt tgttgcgtga
     5581 ggtggtattg cttactgatg atcgcaacct gcgcgttaag gctcttacac gcaacgtccc
     5641 tgtgcgtgac atcccagctt tcttaacatg ggcccaggtc gggggatccg attacaagga
     5701 cgacgatgac aagtaggcta gcgggagcct ctgccctcct cctgcagccc ggatggctct
     5761 cctctgtttt tggtttgctt ccttttgttt gcccttcttt tgggtgaccg gggtggccac
     5821 cattcctgtc cctgcccagt ggggagggga gcaaggcctt ctgccaagag gaggagcatg
     5881 tcccctgcca ctgttgcatg caccccggcc ctgtgactct gtgtgcaagc ccagcagcct
     5941 gtggggcagg gtgccaagag gggacgggac ggacgttcca ggacagaggg agtcgaggat
     6001 gtgatccagg acgggtgggg ggtggaaggg tgggaaaggg caggaaggcc ttggaagcta
     6061 acatgctttc actactggag ggggacggat agagcggatg gcactcgggt ctcactgacg
     6121 ctgttctgaa accatctggc tggctttctg aggtcaggct gggtaggggg ctgtcatcgg
     6181 ccatactgct gatcacactt gccctcatcc gtccagcgat ctcacgctgt tctgtaaata
     6241 cctggtgcta acatcccatg ccgctccccc atggcacccc cagcctcctg cccagttcag
     6301 gctggtccag gaatggatgt gggggatggt cgctgtgtaa cgtgctggtg atctttttcc
     6361 cctacccctt ttatgaccct taaaactttg attttgtcct aaacatgccg ggccaactaa
     6421 agggtgggga gaggagagac gagccccacc atgctcacaa gaaccaacct gcaataaaca
     6481 cttctgtggg ccgccgcgca gagcacgcag cgggcacctc ctgcccctcc atggcgactt
     6541 ggcatcgctt tctgcccgcc tctgccctcg ccctgcctgc cccacccccc agcggagagc
     6601 atgatccgcg cccttgcttc tgactctagc ctctgggaca catcagtcaa cgtgggcagt
     6661 tcagtctggg ttcgttcctt tctctgttct catctggccc cccagggcga tcttgttgct
     6721 ttttgctggg acctgcccag tctagagagg gcctatttcc catgattcct tcatatttgc
     6781 atatacgata caaggctgtt agagagataa ttggaattaa tttgactgta aacacaaaga
     6841 tattagtaca aaatacgtga cgtagaaagt aataatttct tgggtagttt gcagttttaa
     6901 aattatgttt taaaatggac tatcatatgc ttaccgtaac ttgaaagtat ttcgatttct
     6961 tggctttata tatcttgtgg aaaggacgaa acaccggcca gatctgagcc tgggagctct
     7021 ctggccttat tgatacatca tctctgtatt aggctcccaa cttttttgag acgtctagaa
     7081 ttcgatatca agcttatcga taatcaacct ctggattaca aaatttgtga aagattgact
     7141 ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt aatgcctttg
     7201 tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa atcctggttg
     7261 ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt gtgcactgtg
     7321 tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct cctttccggg
     7381 actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg ccttgcccgc
     7441 tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc ggggaaatca
     7501 tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg gacgtccttc
     7561 tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct gctgccggct
     7621 ctgcggcctc ttccgcgtct tcgccttcgc cctcagacga gtcggatctc cctttgggcc
     7681 gcctccccgc atcgataccg tcgacctcga gacctagaaa aacatggagc aatcacaagt
     7741 agcaatacag cagctaccaa tgctgattgt gcctggctag aagcacaaga ggaggaggag
     7801 gtgggttttc cagtcacacc tcaggtacct ttaagaccaa tgacttacaa ggcagctgta
     7861 gatcttagcc actttttaaa agaaaagggg ggactggaag ggctaattca ctcccaacga
     7921 agacaagata tccttgatct gtggatctac cacacacaag gctacttccc tgattggcag
     7981 aactacacac cagggccagg gatcagatat ccactgacct ttggatggtg ctacaagcta
     8041 gtaccagttg agcaagagaa ggtagaagaa gccaatgaag gagagaacac ccgcttgtta
     8101 caccctgtga gcctgcatgg gatggatgac ccggagagag aagtattaga gtggaggttt
     8161 gacagccgcc tagcatttca tcacatggcc cgagagctgc atccggactg tactgggtct
     8221 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
     8281 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
     8341 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagggcc
     8401 cgtttaaacc cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg
     8461 cccctccccc gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata
     8521 aaatgaggaa attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt
     8581 ggggcaggac agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt
     8641 gggctctatg gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc
     8701 gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac
     8761 acttgccagc gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt
     8821 cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc
     8881 tttacggcac ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc
     8941 gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact
     9001 cttgttccaa actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg
     9061 gattttgccg atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc
     9121 gaattaattc tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca
     9181 ggcagaagta tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca
     9241 ggctccccag caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc
     9301 ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc
     9361 catggctgac taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta
     9421 ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga
     9481 gcttgtatat ccattttcgg atctgatcag cacgtgttga caattaatca tcggcatagt
     9541 atatcggcat agtataatac gacaaggtga ggaactaaac catggccaag ttgaccagtg
     9601 ccgttccggt gctcaccgcg cgcgacgtcg ccggagcggt cgagttctgg accgaccggc
     9661 tcgggttctc ccgggacttc gtggaggacg acttcgccgg tgtggtccgg gacgacgtga
     9721 ccctgttcat cagcgcggtc caggaccagg tggtgccgga caacaccctg gcctgggtgt
     9781 gggtgcgcgg cctggacgag ctgtacgccg agtggtcgga ggtcgtgtcc acgaacttcc
     9841 gggacgcctc cgggccggcc atgaccgaga tcggcgagca gccgtggggg cgggagttcg
     9901 ccctgcgcga cccggccggc aactgcgtgc acttcgtggc cgaggagcag gactgacacg
     9961 tgctacgaga tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt
    10021 tccgggacgc cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc
    10081 accccaactt gtttattgca gcttataatg gttacaaata aagcaatagc atcacaaatt
    10141 tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa ctcatcaatg
    10201 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
    10261 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
    10321 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
    10381 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
    10441 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
    10501 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
    10561 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
    10621 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
    10681 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
    10741 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
    10801 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac
    10861 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
    10921 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
    10981 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
    11041 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagaa
    11101 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
    11161 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
    11221 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
    11281 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
    11341 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
    11401 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
    11461 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
    11521 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
    11581 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
    11641 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
    11701 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
    11761 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
    11821 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
    11881 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    11941 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    12001 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    12061 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    12121 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    12181 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    12241 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    12301 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    12361 ataaacaaat aggggttc
//
