LOCUS       sequence-424351-        9842 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  GPCR/G protein BRET biosensor.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9842)
  AUTHORS   Janicot R, Maziarz M, Park JC, Zhao J, Luebbers A, Green E, 
            Philibert CE, Zhang H, Layne MD, Wu JC, Garcia-Marcos M
  TITLE     Direct interrogation of context-dependent GPCR activity with a 
            universal biosensor platform.
  JOURNAL   Cell. 2024 Feb 16:S0092-8674(24)00065-5. doi: 
            10.1016/j.cell.2024.01.028.
  PUBMED    38412860
REFERENCE   2  (bases 1 to 9842)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell. 2024 
            Feb 16:S0092-8674(24)00065-5. doi: 10.1016/j.cell.2024.01.028."
COMMENT     Sequence Label: pLVX-hSyn-Galpha-s ONE-GO
FEATURES             Location/Qualifiers
     source          1..9842
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     misc_feature    254..842
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(307..327)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(725..736)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             1049..1682
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(1810..1826)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1810..1826)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(1823..1845)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    1834..1850
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(1858..1864,1865..1882,1883..1888))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    1903..1924
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(2041..2058)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2212..2800)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2292..2311)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(2971..3762,3763..3831))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3594..3613
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(3832..3936)
                     /gene="bla"
                     /label=AmpR promoter
     polyA_signal    3984..4118
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4021..4040)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4075..4094
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     LTR             4286..4919
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    4966..5091
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    5588..5821
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             6006..6050
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    6313..6428
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     promoter        6470..6917
                     /label=hSyn promoter
                     /note="human synapsin I promoter; confers neuron-specific 
                     expression (Kugler et al., 2003)"
     CDS             join(7259..7261,7262..7972)
                     /codon_start=1
                     /product="Venus YFP with monomerizing A206K mutation (Nagai
                     et al., 2002; Kremers et al., 2006)"
                     /label=mVenus
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     LICTTGKLPVPWPTLVTTLGYGLQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYITADKQKNGIKA
                     NFKIRHNIEDGGVQLADHYQQNTPIGDGPVLLPDNHYLSYQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     primer_bind     complement(7301..7322)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7562..7581)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7909..7930
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(8841..9415,9416..9418,9419..9427)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(9008..9025)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     9235..9254
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             9438..9458
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSK"
     CDS             join(9567..9842,1..240)
                     /codon_start=1
                     /product="NanoLuc(R) luciferase"
                     /label=Nluc
                     /note="human codon-optimized"
                     /translation="MVFTLEDFVGDWRQTAGYNLDQVLEQGGVSSLFQNLGVSVTPIQR
                     IVLSGENGLKIDIHVIIPYEGLSGDQMGQIEKIFKVVYPVDDHHFKVILHYGTLVIDGV
                     TPNMIDYFGRPYEGIAVFDGKKITVTGTLWNGNKIIDERLINPDGSLLFRVTINGVTGW
                     RLCERILA"
ORIGIN
        1 atcctgcact atggcacact ggtaatcgac ggggttacgc cgaacatgat cgactatttc
       61 ggacggccgt atgaaggcat cgccgtgttc gacggcaaaa agatcactgt aacagggacc
      121 ctgtggaacg gcaacaaaat tatcgacgag cgcctgatca accccgacgg ctccctgctg
      181 ttccgagtaa ccatcaacgg agtgaccggc tggcggctgt gcgaacgcat tctggcgtaa
      241 acgcgtctgg aacaatcaac ctctggatta caaaatttgt gaaagattga ctggtattct
      301 taactatgtt gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc
      361 tattgcttcc cgtatggctt tcattttctc ctccttgtat aaatcctggt tgctgtctct
      421 ttatgaggag ttgtggcccg ttgtcaggca acgtggcgtg gtgtgcactg tgtttgctga
      481 cgcaaccccc actggttggg gcattgccac cacctgtcag ctcctttccg ggactttcgc
      541 tttccccctc cctattgcca cggcggaact catcgccgcc tgccttgccc gctgctggac
      601 aggggctcgg ctgttgggca ctgacaattc cgtggtgttg tcggggaagc tgacgtcctt
      661 tccatggctg ctcgcctgtg ttgccacctg gattctgcgc gggacgtcct tctgctacgt
      721 cccttcggcc ctcaatccag cggaccttcc ttcccgcggc ctgctgccgg ctctgcggcc
      781 tcttccgcgt cttcgccttc gccctcagac gagtcggatc tccctttggg ccgcctcccc
      841 gcctggaatt aattctgcag tcgagaccta gaaaaacatg gagcaatcac aagtagcaat
      901 acagcagcta ccaatgctga ttgtgcctgg ctagaagcac aagaggagga ggaggtgggt
      961 tttccagtca cacctcaggt acctttaaga ccaatgactt acaaggcagc tgtagatctt
     1021 agccactttt taaaagaaaa gaggggactg gaagggctaa ttcactccca acgaagacaa
     1081 gatatccttg atctgtggat ctaccacaca caaggctact tccctgatta gcagaactac
     1141 acaccagggc caggggtcag atatccactg acctttggat ggtgctacaa gctagtacca
     1201 gttgagccag ataaggtaga agaggccaat aaaggagaga acaccagctt gttacaccct
     1261 gtgagcctgc atgggatgga tgacccggag agagaagtgt tagagtggag gtttgacagc
     1321 cgcctagcat ttcatcacgt ggcccgagag ctgcatccgg agtacttcaa gaactgctga
     1381 tatcgagctt gctacaaggg actttccgct ggggactttc cagggaggcg tggcctgggc
     1441 gggactgggg agtggcgagc cctcagatcc tgcatataag cagctgcttt ttgcctgtac
     1501 tgggtctctc tggttagacc agatctgagc ctgggagctc tctggctaac tagggaaccc
     1561 actgcttaag cctcaataaa gcttgccttg agtgcttcaa gtagtgtgtg cccgtctgtt
     1621 gtgtgactct ggtaactaga gatccctcag acccttttag tcagtgtgga aaatctctag
     1681 cagtagtagt tcatgtcatc ttattattca gtatttataa cttgcaaaga aatgaatatc
     1741 agagagtgag aggccttgac attgctagcg tttaccgtcg acctctagct agagcttggc
     1801 gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat ccgctcacaa ttccacacaa
     1861 catacgagcc ggaagcataa agtgtaaagc ctggggtgcc taatgagtga gctaactcac
     1921 attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt gccagctgca
     1981 ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct cttccgcttc
     2041 ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat cagctcactc
     2101 aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga acatgtgagc
     2161 aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt ttttccatag
     2221 gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt ggcgaaaccc
     2281 gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt
     2341 tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct
     2401 ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg
     2461 ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta actatcgtct
     2521 tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg gtaacaggat
     2581 tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc ctaactacgg
     2641 ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta ccttcggaaa
     2701 aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg gtttttttgt
     2761 ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt tgatcttttc
     2821 tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg tcatgagatt
     2881 atcaaaaagg atcttcacct agatcctttt aaattaaaaa tgaagtttta aatcaatcta
     2941 aagtatatat gagtaaactt ggtctgacag ttaccaatgc ttaatcagtg aggcacctat
     3001 ctcagcgatc tgtctatttc gttcatccat agttgcctga ctccccgtcg tgtagataac
     3061 tacgatacgg gagggcttac catctggccc cagtgctgca atgataccgc gagacccacg
     3121 ctcaccggct ccagatttat cagcaataaa ccagccagcc ggaagggccg agcgcagaag
     3181 tggtcctgca actttatccg cctccatcca gtctattaat tgttgccggg aagctagagt
     3241 aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc attgctacag gcatcgtggt
     3301 gtcacgctcg tcgtttggta tggcttcatt cagctccggt tcccaacgat caaggcgagt
     3361 tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc ttcggtcctc cgatcgttgt
     3421 cagaagtaag ttggccgcag tgttatcact catggttatg gcagcactgc ataattctct
     3481 tactgtcatg ccatccgtaa gatgcttttc tgtgactggt gagtactcaa ccaagtcatt
     3541 ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg gcgtcaatac gggataatac
     3601 cgcgccacat agcagaactt taaaagtgct catcattgga aaacgttctt cggggcgaaa
     3661 actctcaagg atcttaccgc tgttgagatc cagttcgatg taacccactc gtgcacccaa
     3721 ctgatcttca gcatctttta ctttcaccag cgtttctggg tgagcaaaaa caggaaggca
     3781 aaatgccgca aaaaagggaa taagggcgac acggaaatgt tgaatactca tactcttcct
     3841 ttttcaatat tattgaagca tttatcaggg ttattgtctc atgagcggat acatatttga
     3901 atgtatttag aaaaataaac aaataggggt tccgcgcaca tttccccgaa aagtgccacc
     3961 tgacgtcgac ggatcgggag atcaacttgt ttattgcagc ttataatggt tacaaataaa
     4021 gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct agttgtggtt
     4081 tgtccaaact catcaatgta tcttatcatg tctggatcaa ctggataact caagctaacc
     4141 aaaatcatcc caaacttccc accccatacc ctattaccac tgccaattac ctgtggtttc
     4201 atttactcta aacctgtgat tcctctgaat tattttcatt ttaaagaaat tgtatttgtt
     4261 aaatatgtac tacaaactta gtagttggaa gggctaattc actcccaaag aagacaagat
     4321 atccttgatc tgtggatcta ccacacacaa ggctacttcc ctgattagca gaactacaca
     4381 ccagggccag gggtcagata tccactgacc tttggatggt gctacaagct agtaccagtt
     4441 gagccagata aggtagaaga ggccaataaa ggagagaaca ccagcttgtt acaccctgtg
     4501 agcctgcatg ggatggatga cccggagaga gaagtgttag agtggaggtt tgacagccgc
     4561 ctagcatttc atcacgtggc ccgagagctg catccggagt acttcaagaa ctgctgatat
     4621 cgagcttgct acaagggact ttccgctggg gactttccag ggaggcgtgg cctgggcggg
     4681 actggggagt ggcgagccct cagatcctgc atataagcag ctgctttttg cctgtactgg
     4741 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
     4801 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
     4861 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
     4921 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
     4981 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
     5041 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
     5101 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
     5161 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
     5221 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
     5281 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
     5341 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
     5401 aacaaaagta agaccaccgc acagcaagcg gccggccgct gatcttcaga cctggaggag
     5461 gagatatgag ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac
     5521 cattaggagt agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag
     5581 tgggaatagg agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag
     5641 cgtcaatgac gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga
     5701 acaatttgct gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca
     5761 tcaagcagct ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc
     5821 tggggatttg gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta
     5881 gttggagtaa taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca
     5941 gagaaattaa caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc
     6001 aagaaaagaa tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt
     6061 ttaacataac aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg
     6121 taggtttaag aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt
     6181 caccattatc gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa
     6241 tagaagaaga aggtggagag agagacagag acagatccat tcgattagtg aacggatctc
     6301 gacggtatcg cctttaaaag aaaagggggg attggggggt acagtgcagg ggaaagaata
     6361 gtagacataa tagcaacaga catacaaact aaagaattac aaaaacaaat tacaaaaatt
     6421 caaaattttc gggtttatta cagggacagc agagatccag tttatcgata gtgcaagtgg
     6481 gttttaggac caggatgagg cggggtgggg gtgcctacct gacgaccgac cccgacccac
     6541 tggacaagca cccaaccccc attccccaaa ttgcgcatcc cctatcagag agggggaggg
     6601 gaaacaggat gcggcgaggc gcgtgcgcac tgccagcttc agcaccgcgg acagtgcctt
     6661 cgcccccgcc tggcggcgcg cgccaccgcc gcctcagcac tgaaggcgcg ctgacgtcac
     6721 tcgccggtcc cccgcaaact ccccttcccg gccaccttgg tcgcgtccgc gccgccgccg
     6781 gcccagccgg accgcaccac gcgaggcgcg agataggggg gcacgggcgc gaccatctgc
     6841 gctgcggcgc cggcgactca gcgctgcctc agtctgcggt gggcagcgga ggagtcgtgt
     6901 cgtgcctgag agcgcagctc gagactagtt ctagagcggc cgcggatcca tgggctgcct
     6961 cgggaacagt aagaccgagg accagcgcaa cgaggagaag gcgcagcgtg aggccaacaa
     7021 aaagatcgag aagcagctgc agaaggacaa gcaggtctac cgggccacgc accgcctgct
     7081 gctgctgggt gctggagaat ctggtaaaag caccattgtg aagcagatga ggatcctgca
     7141 tgttaatggg tttaatggag acagtgagaa ggcaaccaaa gtgcaggaca tcaaaaacaa
     7201 cctgaaagag gcgattgaaa ccattgtggc cgccatgagc aacctgggtg gcggaggggt
     7261 gagcaagggc gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga
     7321 cgtaaacggc cacaagttca gcgtgtccgg cgagggcgag ggcgatgcca cctacggcaa
     7381 gctgaccctg aagctgatct gcaccaccgg caagctgccc gtgccctggc ccaccctcgt
     7441 gaccaccctg ggctacggcc tgcagtgctt cgcccgctac cccgaccaca tgaagcagca
     7501 cgacttcttc aagtccgcca tgcccgaagg ctacgtccag gagcgcacca tcttcttcaa
     7561 ggacgacggc aactacaaga cccgcgccga ggtgaagttc gagggcgaca ccctggtgaa
     7621 ccgcatcgag ctgaagggca tcgacttcaa ggaggacggc aacatcctgg ggcacaagct
     7681 ggagtacaac tacaacagcc acaacgtcta tatcaccgcc gacaagcaga agaacggcat
     7741 caaggccaac ttcaagatcc gccacaacat cgaggacggc ggcgtgcagc tcgccgacca
     7801 ctaccagcag aacaccccca tcggcgacgg ccccgtgctg ctgcccgaca accactacct
     7861 gagctaccag tccaagctga gcaaagaccc caacgagaag cgcgatcaca tggtcctgct
     7921 ggagttcgtg accgccgccg ggatcactct cggcatggac gagctgtaca agggcggtgg
     7981 gggagtgccc cccgtggagc tggccaaccc cgagaaccag ttcagagtgg actacattct
     8041 gagtgtgatg aacgtgcctg actttgactt ccctcccgaa ttctatgagc atgccaaggc
     8101 tctgtgggag gatgaaggag tgcgtgcctg ctacgaacgc tccaacgagt accagctgat
     8161 tgactgtgcc cagtacttcc tggacaagat cgacgtgatc aagcaggctg actatgtgcc
     8221 gagcgatcag gacctgcttc gctgccgtgt cctgacttct ggaatctttg agaccaagtt
     8281 ccaggtggac aaagtcaact tccacatgtt tgacgtgggt ggccagcgcg atgaacgccg
     8341 caagtggatc cagtgcttca acgatgtgac tgccatcatc ttcgtggtgg ccagcagcag
     8401 ctacaacatg gtcatccggg aggacaacca gaccaaccgc ctgcaggagg ctctgaacct
     8461 cttcaagagc atctggaaca acagatggct gcgcaccatc tctgtgatcc tgttcctcaa
     8521 caagcaagat ctgctcgctg agaaagtcct tgctgggaaa tcgaagattg aggactactt
     8581 tccagaattt gctcgctaca ctactcctga ggatgctact cccgagcccg gagaggaccc
     8641 acgcgtgacc cgggccaagt acttcattcg agatgagttt ctgaggatca gcactgccag
     8701 tggagatggg cgtcactact gctaccctca tttcacctgc gctgtggaca ctgagaacat
     8761 ccgccgtgtg ttcaacgact gccgtgacat cattcagcgc atgcaccttc gtcagtacga
     8821 gctgctataa gcggccgccg cccctctccc tccccccccc ctaacgttac tggccgaagc
     8881 cgcttggaat aaggccggtg tgcgtttgtc tatatgttat tttccaccat attgccgtct
     8941 tttggcaatg tgagggcccg gaaacctggc cctgtcttct tgacgagcat tcctaggggt
     9001 ctttcccctc tcgccaaagg aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct
     9061 ctggaagctt cttgaagaca aacaacgtct gtagcgaccc tttgcaggca gcggaacccc
     9121 ccacctggcg acaggtgcct ctgcggccaa aagccacgtg tataagatac acctgcaaag
     9181 gcggcacaac cccagtgcca cgttgtgagt tggatagttg tggaaagagt caaatggctc
     9241 tcctcaagcg tattcaacaa ggggctgaag gatgcccaga aggtacccca ttgtatggga
     9301 tctgatctgg ggcctcggtg cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt
     9361 ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg ataagcttgc
     9421 cacaacccgt accaaagatg gggagtagca agagcaagac gtcgaattcc ggaatcaagc
     9481 ttggtggatc atcaggaggt agcagggagc tggcctgggg catcagcgag tggctggagg
     9541 agtggggcag cggaggtggt ggatccatgg tcttcacact cgaagatttc gttggggact
     9601 ggcgacagac agccggctac aacctggacc aagtccttga acagggaggt gtgtccagtt
     9661 tgtttcagaa tctcggggtg tccgtaactc cgatccaaag gattgtcctg agcggtgaaa
     9721 atgggctgaa gatcgacatc catgtcatca tcccgtatga aggtctgagc ggcgaccaaa
     9781 tgggccagat cgaaaaaatt tttaaggtgg tgtaccctgt ggatgatcat cactttaagg
     9841 tg
//
