LOCUS       sequence-424357-        9423 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual-color reporter express eGFP constitutively; tdTomato expression
            is contingent on the exclusion of the frame-shifting cassette exon 
            7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9423)
  AUTHORS   Recinos Y, Ustianenko D, Yeh YT, Wang X, Jacko M, Yesantharao LV, Wu
            Q, Zhang C
  TITLE     CRISPR-dCas13d-based deep screening of proximal and distal 
            splicing-regulatory elements.
  JOURNAL   Nat Commun. 2024 May 7;15(1):3839. doi: 10.1038/s41467-024-47140-8.
  PUBMED    38714659
REFERENCE   2  (bases 1 to 9423)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-05-7"; volume: "15(1):3839. doi"; pages: " 
            10.1038/s41467-024-47140-8"
COMMENT     Sequence Label: pDualEX-SMN2
FEATURES             Location/Qualifiers
     source          1..9423
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             complement(583..648)
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     misc_feature    join(679..687,688..690,691..1265)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(852..871)
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     primer_bind     1081..1098
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     complement(1301..1322)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     CDS             complement(join(1457..1969,1970..1972,1973..1975))
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     1650..1669
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     1909..1930
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(2009..2028)
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     intron          2036..3052
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     complement(2090..2112)
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     promoter        complement(3053..3330)
                     /label=chicken beta-actin promoter
     enhancer        3332..3711
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        3742..3846
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(3847..3915,3916..4707)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(4065..4084)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      4878..5466
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     5367..5386
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     5620..5637
                     /label=L4440
                     /note="L4440 vector, forward primer"
     polyA_signal    5705..5839
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(5729..5748)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     5783..5802
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     promoter        complement(5845..6040)
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      5859..5994
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     protein_bind    6099..6120
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(6135..6140,6141..6158,6159..6165)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    6173..6189
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     6197..6213
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6556..6575
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     polyA_signal    6574..6629
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     6675..6694
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     primer_bind     6796..6812
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     6831..6851
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        6833..6851
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     polyA_signal    6947..7058
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     7047..7064
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     misc_feature    7091..7679
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             7197..7208
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     7606..7626
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     primer_bind     complement(7686..7705)
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     intron          7688..7820
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     CDS             complement(7843..9267)
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="SKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGLV
                     TVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNF
                     EDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLK
                     GEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERS
                     EGRHHLFLYGMDELYK"
ORIGIN
        1 aaaaaaaagt ctgctggtct gcctactagt gatataaaat ggcatcatat cctaaagctc
       61 tttattgtga aagtatgttt cttccacata accaaccagt taagtatgag aattctagta
      121 gggatctaga ttaacctttt atctaatagt tttggcatca aaattcttta atattgattg
      181 ttttacatta acctttcaac tttttaacat ctgaactttt taaatgttca aaaacatttg
      241 ttttccacaa accataaagt tttacaaaag taagattcac tttcataatg ctggcagact
      301 tactccttaa tttaaggaat gctgagcacc ttccttcttt ttgattttgt ctaaaaccct
      361 gtaaggaaaa taaaggaagt taaaaaaaat agctatatag acatagatag ctatatatag
      421 atagctttat atggatgtta aaaagtattt tgtttcacaa gacattttac ttattttatt
      481 caacaaaata tgatcagcaa aacaaagtca cataactaca aaaaaattgt caggaaaaga
      541 tgctgagtga ttacttacca tataatagcc agtatgatag cccttgtcat cgtcatcctt
      601 gtaatcgatg tcatgatctt tataatcacc gtcatggtct ttgtagtcgg gaggtggtgg
      661 gggaattatg ctagccatgg ttgtggccat attatcatcg tgtttttcaa aggaaaacca
      721 cgtccccgtg gttcgggggg cctagacgtt tttttaacct cgactaaaca catgtaaagc
      781 atgtgcaccg aggccccaga tcagatccca tacaatgggg taccttctgg gcatccttca
      841 gccccttgtt gaatacgctt gaggagagcc atttgactct ttccacaact atccaactca
      901 caacgtggca ctggggttgt gccgcctttg caggtgtatc ttatacacgt ggcttttggc
      961 cgcagaggca cctgtcgcca ggtggggggt tccgctgcct gcaaagggtc gctacagacg
     1021 ttgtttgtct tcaagaagct tccagaggaa ctgcttcctt cacgacattc aacagacctt
     1081 gcattccttt ggcgagaggg gaaagacccc taggaatgct cgtcaagaag acagggccag
     1141 gtttccgggc cctcacattg ccaaaagacg gcaatatggt ggaaaataac atatagacaa
     1201 acgcacaccg gccttattcc aagcggcttc ggccagtaac gttagggggg ggggagggag
     1261 aggggcggaa tttctagatc cggtggatcc cggcggcggt cacgaactcc agcaggacca
     1321 tgtgatcgcg cttctcgttg gggtctttgc tcagggcgga ctgggtgctc aggtagtggt
     1381 tgtcgggcag cagcacgggg ccgtcgccga tgggggtgtt ctgctggtag tggtcggcga
     1441 gctgcacgct gccgtcctcg atgttgtggc ggatcttgaa gttcaccttg atgccgttct
     1501 tctgcttgtc ggccatgata tagacgttgt ggctgttgta gttgtactcc agcttgtgcc
     1561 ccaggatgtt gccgtcctcc ttgaagtcga tgcccttcag ctcgatgcgg ttcaccaggg
     1621 tgtcgccctc gaacttcacc tcggcgcggg tcttgtagtt gccgtcgtcc ttgaagaaga
     1681 tggtgcgctc ctggacgtag ccttcgggca tggcggactt gaagaagtcg tgctgcttca
     1741 tgtggtcggg gtagcggctg aagcactgca cgccgtaggt cagggtggtc acgagggtgg
     1801 gccagggcac gggcagcttg ccggtggtgc agatgaactt cagggtcagc ttgccgtagg
     1861 tggcatcgcc ctcgccctcg ccggacacgc tgaacttgtg gccgtttacg tcgccgtcca
     1921 gctcgaccag gatgggcacc accccggtga acagctcctc gcccttgctc accatggtac
     1981 cgaattcttt gccaaaatga tgagacagca caataaccag cacgttgccc aggagctgta
     2041 ggaaaaagaa gaaggcatga acatggttag cagaggctct agagccgccg gtcacacgcc
     2101 agaagccgaa ccccgccctg ccccgtcccc cccgaaggca gccgtccccc cgcggacagc
     2161 cccgaggctg gagagggaga aggggacggc ggcgcggcga cgcacgaagg ccctccccgc
     2221 ccatttcctt cctgccggcg ccgcaccgct tcgccccgcg cccgctagag ggggtgcggc
     2281 ggcgcctccc agatttcggc tccgcacaga tttgggacaa aggaagtccc tgcgccctct
     2341 cgcacgatta ccataaaagg caatggctgc ggctcgccgc gcctcgacag ccgccggcgc
     2401 tccgggggcc gccgcgcccc tcccccgagc cctccccggc ccgaggcggc cccgccccgc
     2461 ccggcacccc cacctgccgc caccccccgc ccggcacggc gagccccgcg ccacgccccg
     2521 tacggagccc cgcacccgaa gccgggccgt gctcagcaac tcggggaggg gggtgcaggg
     2581 ggggttgcag cccgaccgac gcgcccacac cccctgctca cccccccacg cacacacccc
     2641 gcacgcagcc tttgttcccc tcgcagcccc ccccgcaccg cggggcaccg cccccggccg
     2701 cgctcccctc gcgcacactg cggagcgcac aaagccccgc gccgcgcccg cagcgctcac
     2761 agccgccggg cagcgcggag ccgcacgcgg cgctccccac gcacacacac acgcacgcac
     2821 cccccgagcc gctccccccg cacaaagggc cctcccggag cccctcaagg ctttcacgca
     2881 gccacagaaa agaaacaagc cgtcattaaa ccaagcgcta attacagccc ggaggagaag
     2941 ggccgtcccg cccgctcacc tgtgggagta acgcggtcag tcagagccgg ggcgggcggc
     3001 gcgaggcggc ggcggagcgg ggcacggggc gaaggcagcg cgcagcgact cccgcccgcc
     3061 gcgcgcttcg ctttttatag ggccgccgcc gccgccgcct cgccataaaa ggaaactttc
     3121 ggagcgcgcc gctctgattg gctgccgccg cacctctccg cctcgccccg ccccgcccct
     3181 cgccccgccc cgccccgcct ggcgcgcgcc cccccccccc ccccgccccc atcgctgcac
     3241 aaaataatta aaaaataaat aaatacaaaa ttgggggtgg ggaggggggg gagatgggga
     3301 gagtgaagca gaacgtgggg ctcacctcga ccatggtaat agcgatgact aatacgtaga
     3361 tgtactgcca agtaggaaag tcccataagg tcatgtactg ggcataatgc caggcgggcc
     3421 atttaccgtc attgacgtca atagggggcg tacttggcat atgatacact tgatgtactg
     3481 ccaagtgggc agtttaccgt aaatactcca cccattgacg tcaatggaaa gtccctattg
     3541 gcgttactat gggaacatac gtcattattg acgtcaatgg gcgggggtcg ttgggcggtc
     3601 agccaggcgg gccatttacc gtaagttatg taacgcggaa ctccatatat gggctatgaa
     3661 ctaatgaccc cgtaattgat tactattaat aactagtcaa taatcaatgt cgacccaggt
     3721 ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     3781 aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     3841 aagagtatga gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc
     3901 cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg
     3961 ggtgcacgag tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt
     4021 cgccccgaag aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta
     4081 ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat
     4141 gacttggttg agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga
     4201 gaattatgca gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca
     4261 acgatcggag gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact
     4321 cgccttgatc gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc
     4381 acgatgcctg tagcaatggc aacaacgttg cgcaaactat taactggcga actacttact
     4441 ctagcttccc ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt
     4501 ctgcgctcgg cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt
     4561 gggtctcgcg gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt
     4621 atctacacga cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata
     4681 ggtgcctcac tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag
     4741 attgatttaa aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat
     4801 ctcatgacca aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa
     4861 aagatcaaag gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca
     4921 aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt
     4981 ccgaaggtaa ctggcttcag cagagcgcag ataccaaata ctgttcttct agtgtagccg
     5041 tagttaggcc accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc
     5101 ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga
     5161 cgatagttac cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc
     5221 agcttggagc gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc
     5281 gccacgcttc ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca
     5341 ggagagcgca cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg
     5401 tttcgccacc tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta
     5461 tggaaaaacg ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct
     5521 cacatgttct ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag
     5581 tgagctgata ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa
     5641 gcggaagagc gcccaatacg caaaccgcct ctccccgcgc gttggccgat tcattaatgc
     5701 agcggatcca gacatgataa gatacattga tgagtttgga caaaccacaa ctagaatgca
     5761 gtgaaaaaaa tgctttattt gtgaaatttg tgatgctatt gctttatttg taaccattat
     5821 aagctgcaat aaacaagtta gctttttgca aaagcctagg cctccaaaaa agcctcctca
     5881 ctacttctgg aatagctcag aggccgaggc ggcctcggcc tctgcataaa taaaaaaaat
     5941 tagtcagcca tggggcggag aatgggcgga actgggcgga gttaggggcg ggatgggcgg
     6001 agttaggggc gggactatgg ttgctgacta attgagatgc ggatccgctg gcacgacagg
     6061 tttcccgact ggaaagcggg cagtgagcgc aacgcaatta atgtgagtta gctcactcat
     6121 taggcacccc aggctttaca ctttatgctt ccggctcgta tgttgtgtgg aattgtgagc
     6181 ggataacaat ttcacacagg aaacagctat gaccatgatt acgccaagct tgggctgcag
     6241 gtcgagggat ctccataaga gaagagggac agctatgact gggagtagtc aggagaggag
     6301 gaaaaatctg gctagtaaaa catgtaagga aaattttagg gatgttaaag aaaaaaataa
     6361 cacaaaacaa aatataaaaa aaatctaacc tcaagtcaag gcttttctat ggaataagga
     6421 atggacagca gggggctgtt tcatatactg atgacctctt tatagccaac ctttgttcat
     6481 ggcagccagc atatgggcat atgttgccaa actctaaacc aaatactcat tctgatgttt
     6541 taaatgattt gccctcccat atgtccttcc gagtgagaga cacaaaaaat tccaacacac
     6601 tattgcaatg aaaataaatt tcctttatta gccagaagtc agatgctcaa ggggcttcat
     6661 gatgtcccca taatttttgg cagagggaaa aagatctcag tggtatttgt gagccagggc
     6721 attggccaca ccagccacca ccttctgata ggcagcctgc acctgaggag tgaattagcg
     6781 gataacaatt tcacacagga aacagctatg accatgatta cgccaagcgc gcaattaacc
     6841 ctcactaaag ggaacaaaag ctgggctatt gtcttcccaa tcctccccct tgctgtcctg
     6901 ccccacccca ccccccagaa tagaatgaca cctactcaga caatgcgatg caatttcctc
     6961 attttattag gaaaggacag tgggagtggc accttccagg gtcaaggaag gcacggggga
     7021 ggggcaaaca acagatggct ggcaactaga aggcacagtc gaggctgatc gtaccgagct
     7081 cgaattccag gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga
     7141 agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg
     7201 agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg
     7261 agcagccatg gaaaggacgt cagcttcccc gacaacacca cggaattgtc agtgcccaac
     7321 agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata
     7381 gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca
     7441 gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac
     7501 aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata
     7561 cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga
     7621 gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgattg
     7681 tcgacacctg tggagagaaa ggcaaagtgg atgtcagtaa gaccaatagg tgcctatcag
     7741 aaacgcaaga gtcttctctg tctcgacaag cccagtttct attggtctcc ttaaacctgt
     7801 cttgtaacct tgatacttac ctgcggcctc gagttactta agctacttgt acagctcgtc
     7861 catgccgtac aggaacaggt ggtggcggcc ctcggagcgc tcgtactgtt ccacgatggt
     7921 gtagtcctcg ttgtgggagg tgatgtccag cttggtgtcc acgtagtagt agccgggcag
     7981 ttgcacgggc ttcttggcca tgtagatggt cttgaactcc accaggtagt ggccgccgtc
     8041 cttcagcttc agggcctggt ggatctcgcc cttcagcacg ccgtcgcggg ggtacaggcg
     8101 ctcggtggag gcctcccagc ccatggtctt cttctgcatt acggggccgt cgggggggaa
     8161 gttggtgccg cgcatcttca ccttgtagat cagcgtgccg tcctgcaggg aggagtcctg
     8221 ggtcacggtc accagaccgc cgtcctcgaa gttcatcacg cgctcccact tgaagccctc
     8281 ggggaaggac agcttcttgt aatcggggat gtcggcgggg tgcttcacgt acgccttgga
     8341 gccgtacatg aactgggggg acaggatgtc ccaggcgaag ggcagggggc cgcccttggt
     8401 caccttcagc ttggcggtct gggtgccctc gtaggggcgg ccctcgccct cgccctcgat
     8461 ctcgaactcg tggccgttca tggagccctc catgcgcacc ttgaagcgca tgaactcttt
     8521 gatgacggcc atgttgttgt cctcggagga ggcggtgccg gagctgccgc tgccggtgct
     8581 gccggtgcca tgccccagga acaggtggtg gcggccctcg gagcgctcgt actgttccac
     8641 gatggtgtag tcctcgttgt gggaggtgat gtccagcttg gtgtccacgt agtagtagcc
     8701 gggcagttgc acgggcttct tggccatgta gatggtcttg aactccacca ggtagtggcc
     8761 gccgtccttc agcttcaggg cctggtggat ctcgcccttc agcacgccgt cgcgggggta
     8821 caggcgctcg gtggaggcct cccagcccat ggtcttcttc tgcattacgg ggccgtcggg
     8881 ggggaagttg gtgccgcgca tcttcacctt gtagatcagc gtgccgtcct gcagggagga
     8941 gtcctgggtc acggtcacca gaccgccgtc ctcgaagttc atcacgcgct cccacttgaa
     9001 gccctcgggg aaggacagct tcttgtaatc ggggatgtcg gcggggtgct tcacgtacgc
     9061 cttggagccg tacatgaact ggggggacag gatgtcccag gcgaagggca gggggccgcc
     9121 cttggtcacc ttcagcttgg cggtctgggt gccctcgtag gggcggccct cgccctcgcc
     9181 ctcgatctcg aactcgtggc cgttcatgga gccctccatg cgcaccttga agcgcatgaa
     9241 ctctttgatg acctcctcgc ccttgctagc tgccagcatt tcctgcaaat gagaaattag
     9301 aaccagaggc ttgacgaatt ccagttaaac catgtcctct gtggacacca gttaaacttg
     9361 actagagcac ttcatatgtc agagtgtaca gtgcagtatg cctaggttat cccatatcac
     9421 aat
//
