LOCUS       sequence-424358-        9425 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Dual-color reporter express eGFP constitutively; tdTomato expression
            is contingent on the inclusion of the frame-shifting cassette exon 
            7.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9425)
  AUTHORS   Recinos Y, Ustianenko D, Yeh YT, Wang X, Jacko M, Yesantharao LV, Wu
            Q, Zhang C
  TITLE     CRISPR-dCas13d-based deep screening of proximal and distal 
            splicing-regulatory elements.
  JOURNAL   Nat Commun. 2024 May 7;15(1):3839. doi: 10.1038/s41467-024-47140-8.
  PUBMED    38714659
REFERENCE   2  (bases 1 to 9425)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Commun."; date: "2024-05-7"; volume: "15(1):3839. doi"; pages: " 
            10.1038/s41467-024-47140-8"
COMMENT     Sequence Label: pDualIN-SMN2
FEATURES             Location/Qualifiers
     source          1..9425
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             159..1583
                     /codon_start=1
                     /product="tandem dimeric (pseudo-monomeric) derivative of 
                     DsRed (Shaner et al., 2004)"
                     /label=tdTomato
                     /note="mammalian codon-optimized"
                     /translation="SKGEEVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNFEDGGLV
                     TVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLKGEIHQA
                     LKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERSEGRHHL
                     FLGHGTGSTGSGSSGTASSEDNNMAVIKEFMRFKVRMEGSMNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYKKLSFPEGFKWERVMNF
                     EDGGLVTVTQDSSLQDGTLIYKVKMRGTNFPPDGPVMQKKTMGWEASTERLYPRDGVLK
                     GEIHQALKLKDGGHYLVEFKTIYMAKKPVQLPGYYYVDTKLDITSHNEDYTIVEQYERS
                     EGRHHLFLYGMDELYK"
     intron          1606..1738
                     /label=chimeric intron
                     /note="chimera between introns from human beta-globin and 
                     immunoglobulin heavy chain genes"
     primer_bind     1721..1740
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    1747..2335
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1800..1820)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2218..2229)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2362..2379)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    2368..2479
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     primer_bind     complement(2575..2595)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(2575..2593)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(2614..2630)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2732..2751)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    2797..2852
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2851..2870)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(3213..3229)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    3237..3253
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(3261..3267,3268..3285,3286..3291))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    3306..3327
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        3386..3581
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      3432..3567
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     polyA_signal    3587..3721
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3624..3643)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     3678..3697
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(3789..3806)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(3960..4548)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4040..4059)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4719..5510,5511..5579))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5342..5361
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5580..5684)
                     /gene="bla"
                     /label=AmpR promoter
     enhancer        5715..6094
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        6096..6373
                     /label=chicken beta-actin promoter
     intron          6374..7390
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     7314..7336
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     7398..7417
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     CDS             join(7451..7453,7454..7456,7457..7969)
                     /codon_start=1
                     /product="N-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VN173
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIE"
     primer_bind     complement(7496..7517)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7757..7776)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     8104..8125
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     misc_feature    join(8161..8735,8736..8738,8739..8747)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(8328..8345)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     8555..8574
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             8778..8843
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
ORIGIN
        1 attgtgatat gggataacct aggcatactg cactgtacac tctgacatat gaagtgctct
       61 agtcaagttt aactggtgtc cacagaggac atggtttaac tggaattcgt caagcctctg
      121 gttctaattt ctcatttgca ggagaaatgc tggcagctag caagggcgag gaggtcatca
      181 aagagttcat gcgcttcaag gtgcgcatgg agggctccat gaacggccac gagttcgaga
      241 tcgagggcga gggcgagggc cgcccctacg agggcaccca gaccgccaag ctgaaggtga
      301 ccaagggcgg ccccctgccc ttcgcctggg acatcctgtc cccccagttc atgtacggct
      361 ccaaggcgta cgtgaagcac cccgccgaca tccccgatta caagaagctg tccttccccg
      421 agggcttcaa gtgggagcgc gtgatgaact tcgaggacgg cggtctggtg accgtgaccc
      481 aggactcctc cctgcaggac ggcacgctga tctacaaggt gaagatgcgc ggcaccaact
      541 tcccccccga cggccccgta atgcagaaga agaccatggg ctgggaggcc tccaccgagc
      601 gcctgtaccc ccgcgacggc gtgctgaagg gcgagatcca ccaggccctg aagctgaagg
      661 acggcggcca ctacctggtg gagttcaaga ccatctacat ggccaagaag cccgtgcaac
      721 tgcccggcta ctactacgtg gacaccaagc tggacatcac ctcccacaac gaggactaca
      781 ccatcgtgga acagtacgag cgctccgagg gccgccacca cctgttcctg gggcatggca
      841 ccggcagcac cggcagcggc agctccggca ccgcctcctc cgaggacaac aacatggccg
      901 tcatcaaaga gttcatgcgc ttcaaggtgc gcatggaggg ctccatgaac ggccacgagt
      961 tcgagatcga gggcgagggc gagggccgcc cctacgaggg cacccagacc gccaagctga
     1021 aggtgaccaa gggcggcccc ctgcccttcg cctgggacat cctgtccccc cagttcatgt
     1081 acggctccaa ggcgtacgtg aagcaccccg ccgacatccc cgattacaag aagctgtcct
     1141 tccccgaggg cttcaagtgg gagcgcgtga tgaacttcga ggacggcggt ctggtgaccg
     1201 tgacccagga ctcctccctg caggacggca cgctgatcta caaggtgaag atgcgcggca
     1261 ccaacttccc ccccgacggc cccgtaatgc agaagaagac catgggctgg gaggcctcca
     1321 ccgagcgcct gtacccccgc gacggcgtgc tgaagggcga gatccaccag gccctgaagc
     1381 tgaaggacgg cggccactac ctggtggagt tcaagaccat ctacatggcc aagaagcccg
     1441 tgcaactgcc cggctactac tacgtggaca ccaagctgga catcacctcc cacaacgagg
     1501 actacaccat cgtggaacag tacgagcgct ccgagggccg ccaccacctg ttcctgtacg
     1561 gcatggacga gctgtacaag tagcttaagt aactcgaggc cgcaggtaag tatcaaggtt
     1621 acaagacagg tttaaggaga ccaatagaaa ctgggcttgt cgagacagag aagactcttg
     1681 cgtttctgat aggcacctat tggtcttact gacatccact ttgcctttct ctccacaggt
     1741 gtcgacaatc aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat
     1801 gttgctcctt ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct
     1861 tcccgtatgg ctttcatttt ctcctccttg tataaatcct ggttgctgtc tctttatgag
     1921 gagttgtggc ccgttgtcag gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc
     1981 cccactggtt ggggcattgc caccacctgt cagctccttt ccgggacttt cgctttcccc
     2041 ctccctattg ccacggcgga actcatcgcc gcctgccttg cccgctgctg gacaggggct
     2101 cggctgttgg gcactgacaa ttccgtggtg ttgtcgggga agctgacgtc ctttccatgg
     2161 ctgctcgcct gtgttgccac ctggattctg cgcgggacgt ccttctgcta cgtcccttcg
     2221 gccctcaatc cagcggacct tccttcccgc ggcctgctgc cggctctgcg gcctcttccg
     2281 cgtcttcgcc ttcgccctca gacgagtcgg atctcccttt gggccgcctc cccgcctgga
     2341 attcgagctc ggtacgatca gcctcgactg tgccttctag ttgccagcca tctgttgttt
     2401 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
     2461 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
     2521 tggggcagga cagcaagggg gaggattggg aagacaatag cccagctttt gttcccttta
     2581 gtgagggtta attgcgcgct tggcgtaatc atggtcatag ctgtttcctg tgtgaaattg
     2641 ttatccgcta attcactcct caggtgcagg ctgcctatca gaaggtggtg gctggtgtgg
     2701 ccaatgccct ggctcacaaa taccactgag atctttttcc ctctgccaaa aattatgggg
     2761 acatcatgaa gccccttgag catctgactt ctggctaata aaggaaattt attttcattg
     2821 caatagtgtg ttggaatttt ttgtgtctct cactcggaag gacatatggg agggcaaatc
     2881 atttaaaaca tcagaatgag tatttggttt agagtttggc aacatatgcc catatgctgg
     2941 ctgccatgaa caaaggttgg ctataaagag gtcatcagta tatgaaacag ccccctgctg
     3001 tccattcctt attccataga aaagccttga cttgaggtta gatttttttt atattttgtt
     3061 ttgtgttatt tttttcttta acatccctaa aattttcctt acatgtttta ctagccagat
     3121 ttttcctcct ctcctgacta ctcccagtca tagctgtccc tcttctctta tggagatccc
     3181 tcgacctgca gcccaagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
     3241 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
     3301 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
     3361 ggaaacctgt cgtgccagcg gatccgcatc tcaattagtc agcaaccata gtcccgcccc
     3421 taactccgcc catcccgccc ctaactccgc ccagttccgc ccattctccg ccccatggct
     3481 gactaatttt ttttatttat gcagaggccg aggccgcctc ggcctctgag ctattccaga
     3541 agtagtgagg aggctttttt ggaggcctag gcttttgcaa aaagctaact tgtttattgc
     3601 agcttataat ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt
     3661 ttcactgcat tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggat
     3721 ccgctgcatt aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct
     3781 tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca
     3841 gctcactcaa aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac
     3901 atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt
     3961 ttccataggc tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg
     4021 cgaaacccga caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc
     4081 tctcctgttc cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc
     4141 gtggcgcttt ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc
     4201 aagctgggct gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac
     4261 tatcgtcttg agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt
     4321 aacaggatta gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct
     4381 aactacggct acactagaag aacagtattt ggtatctgcg ctctgctgaa gccagttacc
     4441 ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt
     4501 ttttttgttt gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg
     4561 atcttttcta cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc
     4621 atgagattat caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa
     4681 tcaatctaaa gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag
     4741 gcacctatct cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg
     4801 tagataacta cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga
     4861 gacccacgct caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag
     4921 cgcagaagtg gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa
     4981 gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg ttgttgccat tgctacaggc
     5041 atcgtggtgt cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca
     5101 aggcgagtta catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg
     5161 atcgttgtca gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat
     5221 aattctctta ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc
     5281 aagtcattct gagaatagtg tatgcggcga ccgagttgct cttgcccggc gtcaatacgg
     5341 gataataccg cgccacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg
     5401 gggcgaaaac tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt
     5461 gcacccaact gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca
     5521 ggaaggcaaa atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata
     5581 ctcttccttt ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac
     5641 atatttgaat gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa
     5701 gtgccacctg ggtcgacatt gattattgac tagttattaa tagtaatcaa ttacggggtc
     5761 attagttcat agcccatata tggagttccg cgttacataa cttacggtaa atggcccgcc
     5821 tggctgaccg cccaacgacc cccgcccatt gacgtcaata atgacgtatg ttcccatagt
     5881 aacgccaata gggactttcc attgacgtca atgggtggag tatttacggt aaactgccca
     5941 cttggcagta catcaagtgt atcatatgcc aagtacgccc cctattgacg tcaatgacgg
     6001 taaatggccc gcctggcatt atgcccagta catgacctta tgggactttc ctacttggca
     6061 gtacatctac gtattagtca tcgctattac catggtcgag gtgagcccca cgttctgctt
     6121 cactctcccc atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt
     6181 attttgtgca gcgatggggg cggggggggg gggggggcgc gcgccaggcg gggcggggcg
     6241 gggcgagggg cggggcgggg cgaggcggag aggtgcggcg gcagccaatc agagcggcgc
     6301 gctccgaaag tttcctttta tggcgaggcg gcggcggcgg cggccctata aaaagcgaag
     6361 cgcgcggcgg gcgggagtcg ctgcgcgctg ccttcgcccc gtgccccgct ccgccgccgc
     6421 ctcgcgccgc ccgccccggc tctgactgac cgcgttactc ccacaggtga gcgggcggga
     6481 cggcccttct cctccgggct gtaattagcg cttggtttaa tgacggcttg tttcttttct
     6541 gtggctgcgt gaaagccttg aggggctccg ggagggccct ttgtgcgggg ggagcggctc
     6601 ggggggtgcg tgcgtgtgtg tgtgcgtggg gagcgccgcg tgcggctccg cgctgcccgg
     6661 cggctgtgag cgctgcgggc gcggcgcggg gctttgtgcg ctccgcagtg tgcgcgaggg
     6721 gagcgcggcc gggggcggtg ccccgcggtg cggggggggc tgcgagggga acaaaggctg
     6781 cgtgcggggt gtgtgcgtgg gggggtgagc agggggtgtg ggcgcgtcgg tcgggctgca
     6841 accccccctg cacccccctc cccgagttgc tgagcacggc ccggcttcgg gtgcggggct
     6901 ccgtacgggg cgtggcgcgg ggctcgccgt gccgggcggg gggtggcggc aggtgggggt
     6961 gccgggcggg gcggggccgc ctcgggccgg ggagggctcg ggggaggggc gcggcggccc
     7021 ccggagcgcc ggcggctgtc gaggcgcggc gagccgcagc cattgccttt tatggtaatc
     7081 gtgcgagagg gcgcagggac ttcctttgtc ccaaatctgt gcggagccga aatctgggag
     7141 gcgccgccgc accccctcta gcgggcgcgg ggcgaagcgg tgcggcgccg gcaggaagga
     7201 aatgggcggg gagggccttc gtgcgtcgcc gcgccgccgt ccccttctcc ctctccagcc
     7261 tcggggctgt ccgcgggggg acggctgcct tcggggggga cggggcaggg cggggttcgg
     7321 cttctggcgt gtgaccggcg gctctagagc ctctgctaac catgttcatg ccttcttctt
     7381 tttcctacag ctcctgggca acgtgctggt tattgtgctg tctcatcatt ttggcaaaga
     7441 attcggtacc atggtgagca agggcgagga gctgttcacc ggggtggtgc ccatcctggt
     7501 cgagctggac ggcgacgtaa acggccacaa gttcagcgtg tccggcgagg gcgagggcga
     7561 tgccacctac ggcaagctga ccctgaagtt catctgcacc accggcaagc tgcccgtgcc
     7621 ctggcccacc ctcgtgacca ccctgaccta cggcgtgcag tgcttcagcc gctaccccga
     7681 ccacatgaag cagcacgact tcttcaagtc cgccatgccc gaaggctacg tccaggagcg
     7741 caccatcttc ttcaaggacg acggcaacta caagacccgc gccgaggtga agttcgaggg
     7801 cgacaccctg gtgaaccgca tcgagctgaa gggcatcgac ttcaaggagg acggcaacat
     7861 cctggggcac aagctggagt acaactacaa cagccacaac gtctatatca tggccgacaa
     7921 gcagaagaac ggcatcaagg tgaacttcaa gatccgccac aacatcgagg acggcagcgt
     7981 gcagctcgcc gaccactacc agcagaacac ccccatcggc gacggccccg tgctgctgcc
     8041 cgacaaccac tacctgagca cccagtccgc cctgagcaaa gaccccaacg agaagcgcga
     8101 tcacatggtc ctgctggagt tcgtgaccgc cgccgggatc caccggatct agaaattccg
     8161 cccctctccc tccccccccc ctaacgttac tggccgaagc cgcttggaat aaggccggtg
     8221 tgcgtttgtc tatatgttat tttccaccat attgccgtct tttggcaatg tgagggcccg
     8281 gaaacctggc cctgtcttct tgacgagcat tcctaggggt ctttcccctc tcgccaaagg
     8341 aatgcaaggt ctgttgaatg tcgtgaagga agcagttcct ctggaagctt cttgaagaca
     8401 aacaacgtct gtagcgaccc tttgcaggca gcggaacccc ccacctggcg acaggtgcct
     8461 ctgcggccaa aagccacgtg tataagatac acctgcaaag gcggcacaac cccagtgcca
     8521 cgttgtgagt tggatagttg tggaaagagt caaatggctc tcctcaagcg tattcaacaa
     8581 ggggctgaag gatgcccaga aggtacccca ttgtatggga tctgatctgg ggcctcggtg
     8641 cacatgcttt acatgtgttt agtcgaggtt aaaaaaacgt ctaggccccc cgaaccacgg
     8701 ggacgtggtt ttcctttgaa aaacacgatg ataatatggc cacaaccatg gctagcataa
     8761 ttcccccacc acctcccgac tacaaagacc atgacggtga ttataaagat catgacatcg
     8821 attacaagga tgacgatgac aagggctatc atactggcta ttatatggta agtaatcact
     8881 cagcatcttt tcctgacaat ttttttgtag ttatgtgact ttgttttgct gatcatattt
     8941 tgttgaataa aataagtaaa atgtcttgtg aaacaaaata ctttttaaca tccatataaa
     9001 gctatctata tatagctatc tatgtctata tagctatttt ttttaacttc ctttattttc
     9061 cttacagggt tttagacaaa atcaaaaaga aggaaggtgc tcagcattcc ttaaattaag
     9121 gagtaagtct gccagcatta tgaaagtgaa tcttactttt gtaaaacttt atggtttgtg
     9181 gaaaacaaat gtttttgaac atttaaaaag ttcagatgtt aaaaagttga aaggttaatg
     9241 taaaacaatc aatattaaag aattttgatg ccaaaactat tagataaaag gttaatctag
     9301 atccctacta gaattctcat acttaactgg ttggttatgt ggaagaaaca tactttcaca
     9361 ataaagagct ttaggatatg atgccatttt atatcactag taggcagacc agcagacttt
     9421 ttttt
//
