LOCUS       sequence-424367-        4085 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway-compatible Entry vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4085)
  AUTHORS   Weller B, Lin CW, Pogoutse O, Sauer M, Marin-de la Rosa N, Strobel 
            A, Young V, Knapp JJ, Rayhan A, Falter C, Kim DK, Roth FP, 
            Falter-Braun P
  TITLE     A resource of human coronavirus protein-coding sequences in a 
            flexible, multipurpose Gateway Entry clone collection.
  JOURNAL   G3 (Bethesda). 2023 Jul 5;13(7):jkad105. doi: 
            10.1093/g3journal/jkad105.
  PUBMED    37267226
REFERENCE   2  (bases 1 to 4085)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "G3 
            (Bethesda)."; date: "2023-07-5"; volume: "13(7):jkad105. doi"; 
            pages: " 10.1093/g3journal/jkad105"
COMMENT     Sequence Label: pEntry_MERS-CoV-Nucleocapsid
FEATURES             Location/Qualifiers
     source          1..4085
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1396..1495)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(1512..1531)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(1513..1531)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(1536..1552)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(1536..1552)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     CDS             1983..2774
                     /codon_start=1
                     /gene="aadA"
                     /product="aminoglycoside adenylyltransferase (Murphy, 
                     1985)"
                     /label=SmR
                     /note="confers resistance to spectinomycin and 
                     streptomycin"
                     /translation="MREAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
                     SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
                     RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
                     EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
                     VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
     rep_origin      2867..3455
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3356..3375
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3609..3626
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(3785..3812)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(3904..3990)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4039..4061
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4053..4070
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4054..4070
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacaaat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcagaag ggccgtcaag gccaggatcc
      121 atgggatccg catcccctgc tgcacctcgt gctgtttcct ttgccgataa caatgatata
      181 acaaatacaa acctatctcg aggtagagga cgtaatccaa aaccacgagc tgcaccaaat
      241 aacactgtct cttggtacac tgggcttacc caacacggga aagtccctct tacctttcca
      301 cctgggcagg gtgtacctct taatgccaat tctacccctg cgcaaaatgc tgggtattgg
      361 cggagacagg acagaaaaat taataccggg aatggaatta agcaactggc tcccaggtgg
      421 tacttctact acactggaac tggacccgaa gcagcactcc cattccgggc tgttaaggat
      481 ggcatcgttt gggtccatga agatggcgcc actgatgctc cttcaacttt tgggacgcgg
      541 aaccctaaca atgattcagc tattgttaca caattcgcgc ccggtactaa gcttcctaaa
      601 aacttccaca ttgaggggac tggaggcaat agtcaatcat cttcaagagc ctctagctta
      661 agcagaaact cttccagatc tagttcacaa ggttcaagat caggaaactc tacccgcggc
      721 acttctccag gtccatctgg aatcggagca gtaggaggtg atctacttta ccttgatctt
      781 ctgaacagac tacaagccct tgagtctggc aaagtaaagc aatcgcagcc aaaagtaatc
      841 actaagaaag atgctgctgc tgctaaaaat aagatgcgcc acaagcgcac ttccaccaaa
      901 agtttcaaca tggtgcaagc ttttggtctt cgcggaccag gagacctcca gggaaacttt
      961 ggtgatcttc aattgaataa actcggcact gaggacccac gttggcccca aattgctgag
     1021 cttgctccta cagccagtgc ttttatgggt atgtcgcaat ttaaacttac ccatcagaac
     1081 aatgatgatc atggcaaccc tgtgtacttc cttcggtaca gtggagccat taaacttgac
     1141 ccaaagaatc ccaactacaa taagtggttg gagcttcttg agcaaaatat tgatgcctac
     1201 aaaaccttcc ctaagaagga aaagaaacaa aaggcaccaa aagaagaatc aacagaccaa
     1261 atgtctgaac ctccaaagga gcagcgtgtg caaggtagca tcactcagcg cactcgcacc
     1321 cgtccaagtg ttcagcctgg tccaatgatt gatgttaaca ctgatcttaa ttaagacgcg
     1381 atcgcggcct catgggccca gctttcttgt acaaagttgg cattataaaa aataattgct
     1441 catcaatttg ttgcaacgaa caggtcacta tcagtcaaaa taaaatcatt atttgccatc
     1501 cagctgatat cccctatagt gagtcgtatt acatggtcat agctgtttcc tggcagctct
     1561 ggcccgtgtc tcaaaatctc tgatgttaca ttgcacaaga taaaaatata tcatcatgcc
     1621 tcctctagac cagccaggac agaaatgcct cgacttcgct gctacccaag gttgccgggt
     1681 gacgcacacc gtggaaacgg atgaaggcac gaacccagtg gacataagcc tgttcggttc
     1741 gtaagctgta atgcaagtag cgtatgcgct cacgcaactg gtccagaacc ttgaccgaac
     1801 gcagcggtgg taacggcgca gtggcggttt tcatggcttg ttatgactgt ttttttgggg
     1861 tacagtctat gcctcgggca tccaagcagc aagcgcgtta cgccgtgggt cgatgtttga
     1921 tgttatggag cagcaacgat gttacgcagc agggcagtcg ccctaaaaca aagttaaaca
     1981 ttatgaggga agcggtgatc gccgaagtat cgactcaact atcagaggta gttggcgtca
     2041 tcgagcgcca tctcgaaccg acgttgctgg ccgtacattt gtacggctcc gcagtggatg
     2101 gcggcctgaa gccacacagt gatattgatt tgctggttac ggtgaccgta aggcttgatg
     2161 aaacaacgcg gcgagctttg atcaacgacc ttttggaaac ttcggcttcc cctggagaga
     2221 gcgagattct ccgcgctgta gaagtcacca ttgttgtgca cgacgacatc attccgtggc
     2281 gttatccagc taagcgcgaa ctgcaatttg gagaatggca gcgcaatgac attcttgcag
     2341 gtatcttcga gccagccacg atcgacattg atctggctat cttgctgaca aaagcaagag
     2401 aacatagcgt tgccttggta ggtccagcgg cggaggaact ctttgatccg gttcctgaac
     2461 aggatctatt tgaggcgcta aatgaaacct taacgctatg gaactcgccg cccgactggg
     2521 ctggcgatga gcgaaatgta gtgcttacgt tgtcccgcat ttggtacagc gcagtaaccg
     2581 gcaaaatcgc gccgaaggat gtcgctgccg actgggcaat ggagcgcctg ccggcccagt
     2641 atcagcccgt catacttgaa gctagacagg cttatcttgg acaagaagaa gatcgcttgg
     2701 cctcgcgcgc agatcagttg gaagaatttg tccactacgt gaaaggcgag atcaccaagg
     2761 tagtcggcaa ataaccctcg agccacccat gaccaaaatc ccttaacgtg agttacgcgt
     2821 cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     2881 ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     2941 tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     3001 taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     3061 caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     3121 agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     3181 gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     3241 gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     3301 ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     3361 acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     3421 tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     3481 ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     3541 ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     3601 ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc aaaccgcctc
     3661 tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc gactggaaag
     3721 cgggcagtga gcgcaacgca attaatacgc gtaccgctag ccaggaagag tttgtagaaa
     3781 cgcaaaaagg ccatccgtca ggatggcctt ctgcttagtt tgatgcctgg cagtttatgg
     3841 cgggcgtcct gcccgccacc ctccgggccg ttgcttcaca acgttcaaat ccgctcccgg
     3901 cggatttgtc ctactcagga gagcgttcac cgacaaacaa cagataaaac gaaaggccca
     3961 gtcttccgac tgagcctttc gttttatttg atgcctggca gttccctact ctcgcgttaa
     4021 cgctagcatg gatgttttcc cagtcacgac gttgtaaaac gacggccagt cttaagctcg
     4081 ggccc
//
