LOCUS       sequence-424408-        4419 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Gateway entry clone with codon-optimized ORF sequence for subcloning
            into custom expression vectors. Contains a STOP codon at the end of 
            the codon-optimized ORF sequence. .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4419)
  AUTHORS   Superti-Furga G, Lackner D, Wiedmer T, Ingles-Prieto A, Barbosa B, 
            Girardi E, Goldmann U, Gurtl B, Klavins K, Klimek C, Lindinger S, 
            Lineiro-Retes E, Muller AC, Onstein S, Redinger G, Reil D, Sedlyarov
            V, Wolf G, Crawford M, Everley R, ... Steppan CM
  TITLE     The RESOLUTE consortium: unlocking SLC transporters for drug 
            discovery.
  JOURNAL   Nat Rev Drug Discov. 2020 Jul;19(7):429-430. doi: 
            10.1038/d41573-020-00056-6.
  PUBMED    32265506
REFERENCE   2  (bases 1 to 4419)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10"; journalName: 
            "Nat Rev Drug Discov"; date: "2020-07"; volume: "19"; issue: "7"; 
            pages: "429-430"
COMMENT     Sequence Label: pDONR221-SLC24A4_STOP
FEATURES             Location/Qualifiers
     source          1..4419
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    1..100
                     /gene="mutant version of attL"
                     /label=attL1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     protein_bind    complement(1976..2075)
                     /gene="mutant version of attL"
                     /label=attL2
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     primer_bind     complement(2092..2111)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(2093..2111)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(2116..2132)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2116..2132)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2234..2253)
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     CDS             2245..3054
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYGKP
                     DAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGKTA
                     FQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDASD
                     FDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGIAD
                     RYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     complement(2312..2331)
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     rep_origin      3201..3789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     3690..3709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     3943..3960
                     /label=L4440
                     /note="L4440 vector, forward primer"
     terminator      complement(4119..4146)
                     /label=rrnB T2 terminator
                     /note="transcription terminator T2 from the E. coli rrnB 
                     gene"
     terminator      complement(4238..4324)
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     primer_bind     4373..4395
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     4387..4404
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     4388..4404
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 caaataatga ttttattttg actgatagtg acctgttcgt tgcaacacat tgatgagcaa
       61 tgctttttta taatgccaac tttgtacaaa aaagcaggct ccaccatggc cctgagaggc
      121 accctgaggc cactgaaggt gcggagaagg cgcgagatgc tgccacagca agtgggattc
      181 gtgtgcgccg tgctggccct ggtgtgctgt gcctctggcc tgtttggaag cctgggacac
      241 aagaccgcat ccgcctctaa gcgggtgctg cctgacacat ggcggaacag aaagctgatg
      301 gccccagtga acggcaccca gacagccaag aattgtaccg accctgccat ccacgagttc
      361 ccaacagatc tgtttagcaa taaggagaga cagcacggag ccgtgctgct gcacatcctg
      421 ggcgccctgt acatgttcta tgccctggcc atcgtgtgcg acgatttctt tgtgccctct
      481 ctggagaaga tctgtgagag gctgcacctg agcgaggacg tggcaggagc cacctttatg
      541 gcagcaggca gctccacacc tgagctgttc gccagcgtga tcggcgtgtt tatcacccac
      601 ggcgatgtgg gagtgggaac aatcgtggga tccgccgtgt tcaacatcct gtgcatcatc
      661 ggcgtgtgcg gactgtttgc aggacaggtg gtgcgcctga cctggtgggc cgtgtgccgg
      721 gacagcgtgt actatacaat cagcgtgatc gtgctgatcg tgttcatcta cgatgagcag
      781 atcgtgtggt gggagggcct ggtgctgatc atcctgtacg tgttttatat cctgatcatg
      841 aagtataacg tgaagatgca ggccttcttt accgtgaagc agaagagcat cgccaacggc
      901 aatcctgtga actccgagct ggaggccggc aatgacttct acgatggctc ctatgacgat
      961 ccatctgtgc ccctgctggg ccaggtgaag gagaagcccc agtacggcaa gaatcctgtg
     1021 gtcatggtgg acgagatcat gtctagctcc ccccctaagt tcacatttcc cgaggccggc
     1081 ctgagaatca tgatcaccaa caagttcgga cctaggacaa ggctgaggat ggcatccagg
     1141 atcatcatca atgagagaca gaggctgatc aactctgcca atggcgtgtc tagcaagcca
     1201 ctgcagaacg gcagacacga gaatatcgag aacggcaatg tgcccgtgga gaaccctgag
     1261 gatccacagc agaatcagga gcagcagcca ccacctcagc caccacctcc agagccagag
     1321 cctgtggagg cagacttcct gagccccttt tccgtgcctg aggcaagggg cgataaggtg
     1381 aagtgggtgt tcacctggcc cctgatcttt ctgctgtgcg tgacaatccc aaactgttct
     1441 aagccccggt gggagaagtt ctttatggtg accttcatca ccgccacact gtggatcgcc
     1501 gtgttttcct acatcatggt gtggctggtg accatcatcg gctatacact gggcatccct
     1561 gacgtgatca tgggcatcac cttcctggca gcaggcacat ccgtgccaga ttgtatggcc
     1621 tctctgatcg tggcaaggca gggactgggc gacatggccg tgagcaacac catcggctcc
     1681 aacgtgttcg atatcctggt gggactggga gtgccatggg gactgcagac catggtggtg
     1741 aactacggct ccacagtgaa gatcaattct cggggcctgg tgtatagcgt ggtgctgctg
     1801 ctgggaagcg tggccctgac cgtgctggga atccacctga ataagtggcg cctggatcgg
     1861 aagctgggcg tgtacgtgct ggtgctgtat gccatcttcc tgtgcttttc catcatgatc
     1921 gagttcaacg tgttcacatt tgtgaatctg cccatgtgca gggaggacga ttaagaccca
     1981 gctttcttgt acaaagttgg cattataaga aagcattgct tatcaatttg ttgcaacgaa
     2041 caggtcacta tcagtcaaaa taaaatcatt atttgccatc cagctgatat cccctatagt
     2101 gagtcgtatt acatggtcat agctgtttcc tggcagctct ggcccgtgtc tcaaaatctc
     2161 tgatgttaca ttgcacaaga taaaaatata tcatcatgaa caataaaact gtctgcttac
     2221 ataaacagta atacaagggg tgttatgagc catattcaac gggaaacgtc gaggccgcga
     2281 ttaaattcca acatggatgc tgatttatat gggtataaat gggctcgcga taatgtcggg
     2341 caatcaggtg cgacaatcta tcgcttgtat gggaagcccg atgcgccaga gttgtttctg
     2401 aaacatggca aaggtagcgt tgccaatgat gttacagatg agatggtcag actaaactgg
     2461 ctgacggaat ttatgcctct tccgaccatc aagcatttta tccgtactcc tgatgatgca
     2521 tggttactca ccactgcgat ccccggaaaa acagcattcc aggtattaga agaatatcct
     2581 gattcaggtg aaaatattgt tgatgcgctg gcagtgttcc tgcgccggtt gcattcgatt
     2641 cctgtttgta attgtccttt taacagcgat cgcgtatttc gtctcgctca ggcgcaatca
     2701 cgaatgaata acggtttggt tgatgcgagt gattttgatg acgagcgtaa tggctggcct
     2761 gttgaacaag tctggaaaga aatgcataaa cttttgccat tctcaccgga ttcagtcgtc
     2821 actcatggtg atttctcact tgataacctt atttttgacg aggggaaatt aataggttgt
     2881 attgatgttg gacgagtcgg aatcgcagac cgataccagg atcttgccat cctatggaac
     2941 tgcctcggtg agttttctcc ttcattacag aaacggcttt ttcaaaaata tggtattgat
     3001 aatcctgata tgaataaatt gcagtttcat ttgatgctcg atgagttttt ctaatcagaa
     3061 ttggttaatt ggttgtaaca ctggcagagc attacgctga cttgacggga cggcgcaagc
     3121 tcatgaccaa aatcccttaa cgtgagttac gcgtcgttcc actgagcgtc agaccccgta
     3181 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     3241 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     3301 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     3361 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     3421 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     3481 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     3541 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     3601 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     3661 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     3721 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     3781 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     3841 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     3901 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     3961 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     4021 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     4081 acgcgtaccg ctagccagga agagtttgta gaaacgcaaa aaggccatcc gtcaggatgg
     4141 ccttctgctt agtttgatgc ctggcagttt atggcgggcg tcctgcccgc caccctccgg
     4201 gccgttgctt cacaacgttc aaatccgctc ccggcggatt tgtcctactc aggagagcgt
     4261 tcaccgacaa acaacagata aaacgaaagg cccagtcttc cgactgagcc tttcgtttta
     4321 tttgatgcct ggcagttccc tactctcgcg ttaacgctag catggatgtt ttcccagtca
     4381 cgacgttgta aaacgacggc cagtcttaag ctcgggccc
//
