LOCUS       sequence-424410-        4598 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the Streptomyces albireticuli LRD-modifying cytochrome 
            P450 enzyme.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 4598)
  AUTHORS   Lin GM, Voigt CA
  TITLE     Design of a redox-proficient Escherichia coli for screening 
            terpenoids and modifying cytochrome P450s
  JOURNAL   Nature Catalysis. 6, 1016–1029 (2023). 
            https://doi.org/10.1038/s41929-023-01049-5
REFERENCE   2  (bases 1 to 4598)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nature 
            Catalysis. 6, 1016–1029 (2023). 
            https://doi.org/10.1038/s41929-023-01049-5"
COMMENT     Sequence Label: pCYP30
FEATURES             Location/Qualifiers
     source          1..4598
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     rep_origin      complement(296..841)
                     /direction=LEFT
                     /label=p15A ori
                     /note="Plasmids containing the medium-copy-number p15A 
                     origin of replication can be propagated in E. coli cells 
                     that contain a second plasmid with the ColE1 origin.
                     "
     primer_bind     complement(376..395)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(930..1745)
                     /codon_start=1
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     CDS             complement(930..1745)
                     /codon_start=1
                     /gene="aph(3')-Ia"
                     /product="aminoglycoside phosphotransferase"
                     /label=KanR
                     /note="confers resistance to kanamycin in bacteria or G418 
                     (Geneticin(R)) in eukaryotes"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLYG
                     KPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIPGK
                     TAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGLVDA
                     SDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVGRVGI
                     ADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF"
     primer_bind     1653..1672
                     /label=Kan-R
                     /note="Kanamycin resistance gene, reverse primer"
     primer_bind     1737..1756
                     /label=pENTR-R
                     /note="pENTR vectors, reverse primer"
     protein_bind    1922..1949
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     protein_bind    1980..2007
                     /label=CuO
                     /bound_moiety="CymR"
                     /note="CymR-binding P2 operator sequence from the p-cmt 
                     operon of Pseudomonas putida (Mullick et al., 2006)"
     primer_bind     complement(4510..4529)
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
ORIGIN
        1 actgactcgc tgcacgaggc agacctcagc gctagcggag tgtatactgg cttactatgt
       61 tggcactgat gagggtgtca gtgaagtgct tcatgtggca ggagaaaaaa ggctgcaccg
      121 gtgcgtcagc agaatatgtg atacaggata tattccgctt cctcgctcac tgactcgcta
      181 cgctcggtcg ttcgactgcg gcgagcggaa atggcttacg aacggggcgg agatttcctg
      241 gaagatgcca ggaagatact taacagggaa gtgagagggc cgcggcaaag ccgtttttcc
      301 ataggctccg cccccctgac aagcatcacg aaatctgacg ctcaaatcag tggtggcgaa
      361 acccgacagg actataaaga taccaggcgt ttccccctgg cggctccctc gtgcgctctc
      421 ctgttcctgc ctttcggttt accggtgtca ttccgctgtt atggccgcgt ttgtctcatt
      481 ccacgcctga cactcagttc cgggtaggca gttcgctcca agctggactg tatgcacgaa
      541 ccccccgttc agtccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
      601 gaaagacatg caaaagcacc actggcagca gccactggta attgatttag aggagttagt
      661 cttgaagtca tgcgccggtt aaggctaaac tgaaaggaca agttttggtg actgcgctcc
      721 tccaagccag ttacctcggt tcaaagagtt ggtagctcag agaaccttcg aaaaaccgcc
      781 ctgcaaggcg gttttttcgt tttcagagca agagattacg cgcagaccaa aacgatctca
      841 agaagatcat cttattaagg ggtctgacgc tcagtggaac gaaaaatcaa tctaaagtat
      901 atatgagtaa acttggtctg acagttacct tagaaaaact catcgagcat caaatgaaac
      961 tgcaatttat tcatatcagg attatcaata ccatattttt gaaaaagccg tttctgtaat
     1021 gaaggagaaa actcaccgag gcagttccat aggatggcaa gatcctggta tcggtctgcg
     1081 attccgactc gtccaacatc aatacaacct attaatttcc cctcgtcaaa aataaggtta
     1141 tcaagtgaga aatcaccatg agtgacgact gaatccggtg agaatggcaa aagcttatgc
     1201 atttctttcc agacttgttc aacaggccag ccattacgct cgtcatcaaa atcactcgca
     1261 tcaaccaaac cgttattcat tcgtgattgc gcctgagcga gacgaaatac gcgatcgctg
     1321 ttaaaaggac aattacaaac aggaatcgaa tgcaaccggc gcaggaacac tgccagcgca
     1381 tcaacaatat tttcacctga atcaggatat tcttctaata cctggaatgc tgttttcccg
     1441 gggatcgcag tggtgagtaa ccatgcatca tcaggagtac ggataaaatg cttgatggtc
     1501 ggaagaggca taaattccgt cagccagttt agtctgacca tctcatctgt aacatcattg
     1561 gcaacgctac ctttgccatg tttcagaaac aactctggcg catcgggctt cccatacaat
     1621 cgatagattg tcgcacctga ttgcccgaca ttatcgcgag cccatttata cccatataaa
     1681 tcagcatcca tgttggaatt taatcgcggc ctggagcaag acgtttcccg ttgaatatgg
     1741 ctcataacac cccttgtatt actgtttatg taagcagaca gttttattgt tcatgatgat
     1801 atatttttat cttgtgcaat gtacatcaga gattttgaga cacaaccaat tattgaaggc
     1861 ctccctaacg gggggccttt ttttgtttct ggtctcccaa gcgcttaacg atcgttggct
     1921 gaacaaacag acaatctggt ctgtttgtat tatggaaaat ttttctgtat aatagattca
     1981 acaaacagac aatctggtct gtttgtatta tcagcggtca acgcatgtgc tttgcgttct
     2041 gatgagacag tgatgtcgaa accgcctcta caaataattt tgtttagccc ttgccagaag
     2101 gaggtaaact tcatgatgaa tagctccccg ttccccacgc ctgccaaagg cctgccactt
     2161 ctgggccatc ttgtcccact gttaagagat ccccttggtt tcctcgcgcg tctgccggca
     2221 cgcggtgact tagttcaggt acgccttggc cccttacgcg cgctcgtagt ctgtgatcca
     2281 gatcttacct gcgacgtgct gcgcgatgat agaacgttcg acaaaggcgg cccggtatac
     2341 gagcgccttc gtgaatttgc gggaaacggc ttagctacct gtccgtatgg cgatcatcgc
     2401 cgccagcgta gattactgca gccggcattc cacccggcgc gcttaccagg atatgctcgt
     2461 gcggctgcag ctcgcgcggc cgaaaccaca ggctcttggc aagacggtca gatattagat
     2521 gttcttgcgg tcctgcagga gttgaccgtg cgcattacag tcgacacttt gttctctacc
     2581 accgtgccgc cggatctgct gactcaggcc ttgtcggatc tgaacacgtt agtgtcgggc
     2641 ctcactgttc ggatggccac ccctcctccg atggatcgtc tgccgaccct tgggaaccgt
     2701 cgctatcatc aggcacgtgc acgcctgcct cgcctcctgc accgattagt gtccgaacgc
     2761 caggccgccg gtaccgatca tggtgatctg ctgtccatgc tgctgactgc gcaagatcca
     2821 gttccaggcc ccgcggaaca acgccttact gacaccgagg tgatcgacca gattgtgacc
     2881 ttctttctgg gcgctacgga aaccgcggct tcgaccttag cctgggccct tcatctgctt
     2941 ggacagcacc ctgaaaccga acgccgactg cacgctgaag tagacgcagt gctcggtggc
     3001 agaccggcga cccatgccga cctgccccgc ttggaactga ccggtcgcat tctgaccgaa
     3061 acgctgcgcc tgtggcccag tgcttgggga gtgacgcgca cttgcaccgc cgatacgcat
     3121 ttggatcgtt accctgtgcc tgccggtacg acgattgtgt actcgccgta cctgctgcat
     3181 catcgtgccg acttataccc agacccggag cgcttcgatc cggatcgttg ggcgcctgaa
     3241 aacccccgtc ccccacggcg tgaggcatat atccctttcg gcgggggcgc acgcaaatgc
     3301 attggtgaac gtttcgttac gagcgaaatg gtcttagccc tggcaaccat tgctacccgc
     3361 tggcgcctgc gaccgttacc gggaacgcgt gtacgcccga ctattggtgg tcccactttg
     3421 aacccatcag gcttacgcat gtgtgccata gcgagaacga cgaccggtat tagctcgccg
     3481 gcaaacagag aaaaaccgca gtaataagga tgagctctac aaataagcag aggtggttgt
     3541 gttgcgaaaa aaaaaaaaaa acaccctaac gggtgttttt ttttttttgg tgtccccacg
     3601 tgtggcgctg gagaccgtcc aatggcggcg cgccatcgaa tggtgcaaaa cctttcgcgg
     3661 tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatatga gcccgaaacg
     3721 tcgtacccag gcagaacgtg caatggaaac ccagggtaaa ctgattgcag cagcactggg
     3781 tgttctgcgt gaaaaaggtt atgcaggttt tcgtattgca gatgttccgg gtgcagccgg
     3841 tgttagccgt ggtgcacaga gccatcattt tccgaccaaa ctggaactgc tgctggcaac
     3901 ctttgaatgg ctgtatgagc agattaccga acgtagccgt gcacgtctgg caaaactgaa
     3961 accggaagat gatgttattc agcagatgct ggatgatgca gcagattttt ttctggatga
     4021 tgattttagc atcggcctgg atctgattgt tgcagcagat cgtgatccgg cactgcgtga
     4081 aggtattctg cgtaccgttg aacgtaatcg ttttgttgtt gaagatatgt ggctgggtgt
     4141 gctggtgagc cgtggtctga gccgtgatga tgccgaagat attctgtggc tgatttttaa
     4201 cagcgttcgt ggtctgacag ttcgtagcct gtggcagaaa gataaagaac gttttgaacg
     4261 tgtgcgtaat agcaccctgg aaattgcacg tgaacgttat gcaaaattca aacgttgata
     4321 aggatcctaa ttggtaacga atcagacaat tgacggctcg agggagtagc atagggtttg
     4381 cagaatccct gcttcgtcca tttgacaggc acattatgca tcgatgataa gctgtcaaac
     4441 atgagcagat cctctacgcc ggacgcatcg tggccggcat caccggcgcc acaggtgcgg
     4501 ttgctggcgc ctatatcgcc gacatcaccg atggggaaga tcgggctcgc cacttcgggc
     4561 tcatgagcaa atattttatc tgaggtgctt cctcgctc
//
