LOCUS       sequence-424415-        7754 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7754)
  AUTHORS   Young TL, Cepko CL
  TITLE     A role for ligand-gated ion channels in rod photoreceptor 
            development.
  JOURNAL   Neuron. 2004 Mar 25. 41(6):867-79.
  PUBMED    15046720
REFERENCE   2  (bases 1 to 7754)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Neuron. 
            2004 Mar 25. 41(6):867-79."
COMMENT     Sequence Label: pGFP Glra2 (CC#301)
FEATURES             Location/Qualifiers
     source          1..7754
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     CDS             138..554
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     484..506
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     522..538
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     misc_feature    564..938
                     /label=pol region
                     /note="Moloney murine leukemia virus (MMLV) pol region 
                     containing the splice acceptor site"
     primer_bind     876..892
                     /label=pBMN 5'
                     /note="MMLV sequence, for inserts in pBMN retroviral 
                     vector"
     misc_feature    join(2420..2994,2995..2997,2998..3006)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(2587..2604)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     2814..2833
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(3007..3009,3010..3012,3013..3726)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     CDS             join(3007..3009,3010..3012,3013..3723)
                     /codon_start=1
                     /product="enhanced GFP"
                     /label=EGFP
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(3052..3073)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(3313..3332)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     3660..3681
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             3912..4381
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     primer_bind     complement(4511..4528)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(4682..5270)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(4762..4781)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             join(5394..5462,5463..6254)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5612..5631)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     LTR             6936..7405
                     /note="long terminal repeat from Moloney murine leukemia 
                     virus"
     misc_feature    join(7470..7754,1..73)
                     /label=MMLV Psi
                     /note="packaging signal of Moloney murine leukemia virus 
                     (MMLV)"
ORIGIN
        1 aacagttccc gcctccgtct gaatttttgc tttcggtttg ggaccgaagc cgcgccgcgc
       61 gtcttgtctg ctgcagcatc gttctgtgtt gtctctgtct gactgtgttt ctgtatttgt
      121 ctgaaaatat gggcccgggc cagactgtta ccactccctt aagtttgacc ttaggtcact
      181 ggaaagatgt cgagcggatc gctcacaacc agtcggtaga tgtcaagaag agacgttggg
      241 ttaccttctg ctctgcagaa tggccaacct ttaacgtcgg atggccgcga gacggcacct
      301 ttaaccgaga cctcatcacc caggttaaga tcaaggtctt ttcacctggc ccgcatggac
      361 acccagacca ggtcccctac atcgtgacct gggaagcctt ggcttttgac ccccctccct
      421 gggtcaagcc ctttgtacac cctaagcctc cgcctcctct tcctccatcc gccccgtctc
      481 tcccccttga acctcctcgt tcgaccccgc ctcgatcctc cctttatcca gccctcactc
      541 cttctctagg cgcccccata tggccatatg agatcttata tggggcaccc ccgccccttg
      601 taaacttccc tgaccctgac atgacaagag ttactaacag cccctctctc caagctcact
      661 tacaggctct ctacttagtc cagcacgaag tctggagacc tctggcggca gcctaccaag
      721 aacaactgga ccgaccggtg gtacctcacc cttaccgagt cggcgacaca gtgtgggtcc
      781 gccgacacca gactaagaac ctagaacctc gctggaaagg accttacaca gtcctgctga
      841 ccacccccac cgccctcaaa gtagacggca tcgcagcttg gatacacgcc gcccacgtga
      901 aggctgccga ccccgggggt ggaccatcct ctagactgcc ggatccattt aaattcgaat
      961 tcctgcaggc ctcgagggcc ggcgcgccgc ggccgctaca gcttgatatc gaattcgaat
     1021 gaaccggcag ctagtgaaca ttttgacagc cttgtttgca tttttcttag agacaaacca
     1081 cttcaggacg gctttctgca aagaccatga ctccaggtct ggaaaacaac cttcacagac
     1141 cctatctcct tcagatttct tggacaagtt aatgggaagg acatcaggat atgatgcaag
     1201 aatcaggcca aattttaaag gtcctccagt aaacgttact tgcaatattt ttatcaacag
     1261 ttttggatca gtcacagaaa cgaccatgga ctaccgagtg aatatttttc tgagacaaca
     1321 gtggaatgat tcacggctgg cgtacagtga gtacccagat gactccctgg acttggaccc
     1381 atccatgcta gactccattt ggaaaccaga tttgttcttt gccaatgaga agggtgccaa
     1441 cttccacgat gtcaccactg acaacaaatt gctacggatt tcgaaaaatg gcaaagtgct
     1501 ctacagtatc agactcacct tgaccttatc ctgtcccatg gacttgaaga actttccgat
     1561 ggatgtccag acctgtacaa tgcagctgga gagttttggg tacacgatga atgacctgat
     1621 atttgagtgg ttaagtgatg gtccagtgca agttgctgaa ggattgaccc tgccccagtt
     1681 tattttgaaa gaagagaagg aacttggcta ctgtacaaag cactacaaca ctggaaagtt
     1741 tacctgcatt gaggtcaagt ttcacctgga acgccaaatg ggatattatt tgatccagat
     1801 gtacatccca agcctgctta tagtaatttt gtcctgggtt tccttttgga taaatatgga
     1861 tgcagcccct gccagggtcg cactgggcat caccacagtc ttaacgatga ccacccagag
     1921 ttcaggctcc agggcatctc tgccaaaggt ctcctatgta aaagcgattg acatctggat
     1981 ggcggtgtgc cttctgtttg tgtttgctgc cttactggaa tacgcagcgg tgaacttcgt
     2041 ctccaggcaa cacaaggagt tcctgcgcct ccgaagaaga cagaagaggc agaataagga
     2101 agaagacgtt actcgtgaaa gtcgttttaa ttttagcggt tatgggatgg gtcactgcct
     2161 ccaagtgaaa gatggaacag ctgtcaaggc cacacctgcc aacccactcc cacaaccgcc
     2221 aaaagatgga gatgctatca agaagaagtt tgtggaccgg gcaaaaagga ttgacacgat
     2281 atctcgagct gccttcccat tggccttcct cattttcaac atcttttact ggaccacata
     2341 caagatcatt cggcatgaag atgtccacaa gaaataggaa ttcctgcagc ccgggggatc
     2401 gtaaattccg cccccccccc ccctctccct cccccccccc taacgttact ggccgaagcc
     2461 gcttggaata aggccggtgt gcgtttgtct atatgttatt ttccaccata ttgccgtctt
     2521 ttggcaatgt gagggcccgg aaacctggcc ctgtcttctt gacgagcatt cctaggggtc
     2581 tttcccctct cgccaaagga atgcaaggtc tgttgaatgt cgtgaaggaa gcagttcctc
     2641 tggaagcttc ttgaagacaa acaacgtctg tagcgaccct ttgcaggcag cggaaccccc
     2701 cacctggcga caggtgcctc tgcggccaaa agccacgtgt ataagataca cctgcaaagg
     2761 cggcacaacc ccagtgccac gttgtgagtt ggatagttgt ggaaagagtc aaatggctct
     2821 cctcaagcgt attcaacaag gggctgaagg atgcccagaa ggtaccccat tgtatgggat
     2881 ctgatctggg gcctcggtgc acatgcttta catgtgttta gtcgaggtta aaaaaacgtc
     2941 taggcccccc gaaccacggg gacgtggttt tcctttgaaa aacacgatga taatatggcc
     3001 acaaccatgg tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat cctggtcgag
     3061 ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga gggcgatgcc
     3121 acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc cgtgccctgg
     3181 cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta ccccgaccac
     3241 atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca ggagcgcacc
     3301 atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt cgagggcgac
     3361 accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg caacatcctg
     3421 gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc cgacaagcag
     3481 aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg cagcgtgcag
     3541 ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct gctgcccgac
     3601 aaccactacc tgagcaccca gtccgccctg agcaaagacc ccaacgagaa gcgcgatcac
     3661 atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga cgagctgtac
     3721 aagtaaagcg ccagcacagt ggtcgacgat aaaataaaag attttattta gtctccagaa
     3781 aaagggggga atgaaagacc ccacctgtag gtttggcaag ctagcttaag taacgccatt
     3841 ttgcaaggca tggaaaaata cataactgag aatagagaag ttcagatcaa ggtcaggaac
     3901 agatggaaca gctgaatatg ggccaaacag gatatctgtg gtaagcagtt cctgccccgg
     3961 ctcagggcca agaacagatg gaacagctga atatgggcca aacaggatat ctgtggtaag
     4021 cagttcctgc cccggctcag ggccaagaac agatggtccc cagatgcggt ccagccctca
     4081 gcagtttcta gagaaccatc agatgtttcc agggtgcccc aaggacctga aatgaccctg
     4141 tgccttattt gaactaacca atcagttcgc ttctcgcttc tgttcgcgcg cttctgctcc
     4201 ccgagctcaa taaaagagcc cacaacccct cactcggggc gccagtcctc cgattgactg
     4261 agtcgcccgg gtacccgtgt atccaataaa ccctcttgca gttgcatccg acttgtggtc
     4321 tcgctgttcc ttgggagggt ctcctctgag tgattgacta cccgtcagcg ggggtctttc
     4381 acatgcagca tgtatcaaaa ttaatttggt tttttttctt aagtatttac attaaatggc
     4441 catagttgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgct
     4501 cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg cgagcggtat
     4561 cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac gcaggaaaga
     4621 acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg ttgctggcgt
     4681 ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca agtcagaggt
     4741 ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc tccctcgtgc
     4801 gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc ccttcgggaa
     4861 gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag gtcgttcgct
     4921 ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc ttatccggta
     4981 actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca gcagccactg
     5041 gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg aagtggtggc
     5101 ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg aagccagtta
     5161 ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct ggtagcggtg
     5221 gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa gaagatcctt
     5281 tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa gggattttgg
     5341 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
     5401 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
     5461 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
     5521 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
     5581 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
     5641 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
     5701 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
     5761 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
     5821 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
     5881 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
     5941 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
     6001 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
     6061 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
     6121 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
     6181 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
     6241 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttgcggc
     6301 cggccgcaaa aggatctagg tgaagatcct ttttgataat ctcatgagcg gatacatatt
     6361 tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     6421 accagctttg ctcttaggag tttcctaata catcccaaac tcaaatatat aaagcatttg
     6481 acttgttcta tgccctaggg ggcgggggga agctaagcca gcttttttta acatttaaaa
     6541 tgttaattcc attttaaatg cacagatgtt tttatttcat aagggtttca atgtgcatga
     6601 atgctgcaat attcctgtta ccaaagctag tataaataaa aatagataaa cgtggaaatt
     6661 acttagagtt tctgtcatta acgtttcctt cctcagttga caacataaat gcgctgctga
     6721 gaagccagtt tgcatctgtc aggatcaatt tcccattatg ccagtcatat taattactag
     6781 tcaattagtt gatttttatt tttgacatat acatgtgaaa gaccccacct gtaggtttgg
     6841 caagctagct taagtaacgc cattttgcaa ggcatggaaa aatacataac tgagaataga
     6901 aaagttcaga tcaaggtcag gaacagatgg aacagctgaa tatgggccaa acaggatatc
     6961 tgtggtaagc agttcctgcc ccggctcagg gccaagaaca gatggaacag ctgaatatgg
     7021 gccaaacagg atatctgtgg taagcagttc ctgccccggc tcagggccaa gaacagatgg
     7081 tccccagatg cggtccagcc ctcagcagtt tctagagaac catcagatgt ttccagggtg
     7141 ccccaaggac ctgaaatgac cctgtgcctt atttgaacta accaatcagt tcgcttctcg
     7201 cttctgttcg cgcgcttctg ctccccgagc tcaataaaag agcccacaac ccctcactcg
     7261 gcgcgccagt cctccgattg actgagtcgc ccgggtaccc gtgtatccaa taaaccctct
     7321 tgcagttgca tccgacttgt ggtctcgctg ttccttggga gggtctcctc tgagtgattg
     7381 actacccgtc agcgggggtc tttcatttgg gggctcgtcc gggatcggga gacccctgcc
     7441 cagggaccac cgacccacca ccgggaggta agctggccag caacttatct gtgtctgtcc
     7501 gattgtctag tgtctatgac tgattttatg cgcctgcgtc ggtactagtt agctaactag
     7561 ctctgtatct ggcggacccg tggtggaact gacgagttcg gaacacccgg ccgcaaccct
     7621 gggagacgtc ccagggactt cgggggccgt ttttgtggcc cgacctgagt ccaaaaatcc
     7681 cgatcgtttt ggactctttg gtgcaccccc cttagaggag ggatatgtgg ttctggtagg
     7741 agacgagaac ctaa
//
