LOCUS       sequence-424429-        3240 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3240)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 3240)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRB1 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..3240
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1195..1215
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQS"
     CDS             3193..3216
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aaagtacgtt cgcgatcctg gcgcagctga gcgccgatgc taacatggag
     1261 aaggctacac tgcccagtgt cacactgatc gtgggttgtg gagtgagctc tctcacgctc
     1321 ctgatgctgg tcatcattta tgtgagcgtg tggcgctata ttcgcagtga acgcagcgtc
     1381 attctgatca acttttgcct tagcattatc tcaagcaacg cgctgatcct catcggccaa
     1441 acgcagacac gtaacaaggt tgtatgtaca ctggtggccg cattcctcca tttctttttc
     1501 ctgtcttcct tctgttgggt tttgactgag gcctggcaat cctacatggc tgtgaccggg
     1561 catctgcgta accgtttgat ccggaagcgg tttctgtgct tgggatgggg attgcccgct
     1621 ctggtcgtgg ctattagtgt aggtttcaca aaggcgaagg gttatagcac catgaactat
     1681 tgctggctgt ctctcgaagg aggcttgctg tatgcttttg tgggacccgc tgccgctgtt
     1741 gtcctggtca acatggtgat tggtattctg gtgtttaata agctcgtatc taaggacggc
     1801 attaccgaca aaaagctgaa ggaacgcgct ggagccagcc tctggtccag ttgcgtcgtg
     1861 ctcccacttc tcgcactcac gtggatgagc gccgtcctcg ccgtgaccga ccggcgctcc
     1921 gccctgtttc aaattctgtt cgcagtgttt gactctctgg agggcttcgt tatcgtgatg
     1981 gtccactgta tccttaggcg tgaggtgcag gatgctgtga agtgccgtgt cgtagacagg
     2041 caggaagagg gtaatggcga ttccggtggc tctttccaga acggtcacgc ccagcttatg
     2101 acagacttcg agaaggacgt ggatcttgcg tgccgctctg tgctcaacaa ggacatcgca
     2161 gcgtgtcgga ctgcaaccat caccggcacc ctgaaaagac ctagcctccc ggaagaggaa
     2221 aagctgaagc tggctcatgc gaagggaccg cccacaaatt ttaattctct gcctgctaat
     2281 gtcagtaagc tccacctgca cggcagccca cgctaccccg gtggccctct ccccgatttt
     2341 cctaaccact cactgaccct gaaacgggat aaggctccca aaagctcttt cgtgggagac
     2401 ggtgacatct ttaaaaagct cgatagcgag ctgagccgtg cccaagagaa ggccctggat
     2461 acatcttacg tgattctccc aacagccaca gccacgctgc gcccaaagcc taaggaagag
     2521 ccaaaatact ctattcacat cgatcagatg ccccagacca gacttatcca cctgtccact
     2581 gcccctgagg caagtctgcc cgcgcgcagc ccaccttcta gacagccgcc cagtggtggc
     2641 ccgcctgaag caccgccagc gcaaccgcca ccgcctccac ctccgcctcc accgcctccg
     2701 cagcaaccac tgccacctcc gccaaacctt gaacctgccc ctccgtccct gggtgatccc
     2761 ggagagccgg cggcccaccc cggtccctca accggaccct ccaccaagaa tgagaacgtt
     2821 gccaccttgt ccgtttcctc attggaaagg agaaagtcta gatatgccga actcgacttt
     2881 gagaagatta tgcacacccg gaagcgccat caggacatgt tccaggacct gaaccggaaa
     2941 ctccagcacg ccgctgaaaa agacaaagag gtgctgggtc cggactcaaa gccagagaaa
     3001 cagcaaactc ccaataagag gccttgggag tccctgcgca aggcacatgg aaccccaacc
     3061 tgggttaaaa aggagcttga gcctcttcag ccaagcccac tcgaactgcg ctccgtcgag
     3121 tgggagagat ctggcgcgac tatcccactc gtcggccagg atattatcga cctgcaaaca
     3181 gaggtgggtt ctgactacaa agacgatgac gacaagtgaa gatctgacaa aactcacaca
//
