LOCUS       sequence-424432-        2967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRC1 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..2967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2920..2943
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aaactgccag cttcgccgtg ctgatggaca tcagccgtag agagaacgga
     1261 gaagtgctgc ctctgaaaat cgtgacatac gccgccgtgt ccctctccct ggctgccctg
     1321 ctggtggctt tcgtgctgct gtctctggtg cggatgcttc ggagcaacct gcatagcatc
     1381 cacaagcacc tggccgtggc cctgttcctg agccagctgg tctttgtgat tggcatcaac
     1441 cagacagaga atcctttcct gtgtacagtg gtggccatcc tgctccacta tatctacatg
     1501 agcacattcg cctggactct ggtggaaagc ctgcacgtgt acagaatgct gacagaggtg
     1561 cgcaacattg acaccggccc tatgcggttc tactacgtgg tcggatgggg catccccgct
     1621 atcgtgaccg gcctggccgt gggcttggat ccccagggct acggcaaccc cgacttctgc
     1681 tggctgagct tgcaggacac cctgatctgg tctttcgccg gccccatcgg cgccgtaatc
     1741 atcatcaaca ccgttacatc tgtgctgtct gctaaggtgt cctgtcagag aaagcaccac
     1801 tactacggaa aaaagggcat cgtgagcctg ctgcgaaccg ccttcctgct actgctcctg
     1861 atcagcgcca catggctgct gggcctgctg gccgtgaaca gagatgccct gagctttcac
     1921 tacctgtttg ccatcttcag cggcctgcag ggccccttcg tgctgctgtt ccactgcgtg
     1981 ctgaaccagg aggtgagaaa gcacctgaag ggcgtgctgg gaggcaggaa gctgcacctg
     2041 gaagattccg ccaccacaag agccaccctg ctgaccagaa gcctgaactg caacaccacc
     2101 ttcggcgacg gacctgacat gctgagaaca gatctgggcg agtctaccgc cagcctggac
     2161 tccatcgtgc gggacgaggg catccagaaa ctgggagttt cctccggcct ggtgcgggga
     2221 tcccacggcg aacccgacgc cagcctcatg cctcggagct gcaaggaccc accaggccac
     2281 gacagcgatt ccgacagcga gctgagcctg gacgagcaga gcagcagcta cgcctctagc
     2341 cacagctctg atagcgagga cgacggcgtg ggcgccgagg aaaagtggga ccccgccaga
     2401 ggcgccgtgc acagcacccc taagggcgat gccgttgcta atcacgtgcc agctggctgg
     2461 cctgatcaat ctctggctga gagcgacagc gaggacccca gcggcaagcc tagactgaag
     2521 gtggaaacca aggtgtctgt ggaactgcac agagaggaac aagggagcca tagaggcgag
     2581 taccctcctg atcaggagag cggcggcgcg gctagactgg cctccagcca gcctcctgag
     2641 cagcggaaag gcatcctgaa gaacaaggtc acatatcctc ctccactgac cctgaccgag
     2701 cagaccctga agggaagact gagagaaaag ctggctgatt gcgagcaaag tcctaccagc
     2761 tctagaacca gctccctggg aagcggaggt cctgattgcg ccatcacagt gaagagcccc
     2821 ggcagagaac ctggccggga ccacctaaat ggcgtcgcca tgaacgtgcg gaccggctct
     2881 gcccaggccg acggcagcga ctctgaaaaa cctggttctg actacaaaga cgatgacgac
     2941 aagtgaagat ctgacaaaac tcacaca
//
