LOCUS       sequence-424433-        2967 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2967)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 2967)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRC2 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..2967
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2920..2943
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aaacatcatt cgccgtactc atggacgttt cccgtcgcga aaacggcgag
     1261 atcctgcctc tgaaaacttt gacctacgtg gcgctcggag tcactctcgc cgcattgctc
     1321 ctgaccttct ttttcctgac cttgctgagg atcctccgtt ctaaccagca tggtattcgt
     1381 aggaacctga cagcggccct gggattggcc cagctcgtct tcctcctggg catcaaccag
     1441 gcagacctgc ccttcgcctg cacagtgatc gcgatcttgc tgcacttcct gtatttgtgc
     1501 accttctctt gggccctcct ggaggctctg cacctgtaca gggctcttac agaggttcgc
     1561 gatgtcaaca caggacctat gcgcttctat tacatgttgg gctggggcgt cccagcgttc
     1621 atcaccggcc tcgccgttgg tctggacccc gagggttacg ggaaccccga cttctgttgg
     1681 ctctccatct acgacaccct gatctggtcc ttcgccggac cagtggcgtt cgctgtgtcc
     1741 atgtccgtct tcctttacat cctcgcagcc cgggcctcct gtgccgcaca gaggcaggga
     1801 ttcgaaaaga aaggcccagt aagcggactg cagccttcct tcgccgttct gttgctgctc
     1861 tccgccacat ggcttctcgc actcctgagc gtcaatagcg acacgttgct gtttcactat
     1921 cttttcgcta cctgtaactg tatccagggc cccttcatct ttttgtctta cgtggttctg
     1981 tccaaagaag tcagaaaggc tctgaagctc gcctgcagcc gcaagcctag cccagatccc
     2041 gccctgacta ccaagtccac tctcaccagt agctacaact gccctagccc ctacgctgat
     2101 ggcagactct atcagccata tggtgattct gccgggtccc tgcactccac tagccggtca
     2161 ggcaagtccc agccatccta catcccgttc ctcctgcgcg aagagtccgc cctgaacccg
     2221 ggccaaggtc caccgggcct gggtgacccc ggatcactct tcctggaggg acaggaccaa
     2281 cagcatgacc ctgataccga ctccgattcc gatttgtccc tcgaggatga ccagtctggg
     2341 tcatacgcct ctacccattc ctctgacagc gaagaggagg aagaggaaga agaggaagag
     2401 gcggccttcc caggagagca gggctgggat tcccttctgg gccccggcgc tgaacgcctg
     2461 cctctccata gcactccgaa agacggcggt cccggaccgg gtaaggcccc ctggcctggg
     2521 gatttcggca cgaccgccaa ggagagctcc ggcaacggcg ccccagagga acgtcttagg
     2581 gagaacggcg atgccctgag ccgtgaaggt agcctgggcc ctttgccagg tagcagtgct
     2641 caaccccata agggcatctt gaaaaagaaa tgtctcccaa ctatctccga gaagtcctct
     2701 ttgctgagat tgcctctgga acagtgcact ggcagctctc gcggtagttc tgcatccgaa
     2761 ggctcccggg gaggtcctcc gcctcggccc cctccgcgcc agtctttgca ggagcaactg
     2821 aacggcgtga tgcctattgc catgtctatt aaggcgggga ccgtcgacga agatagttcc
     2881 ggttccgaat ttcttttttt caacttcctg catggttctg actacaaaga cgatgacgac
     2941 aagtgaagat ctgacaaaac tcacaca
//
