LOCUS       sequence-424434-        3654 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3654)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 3654)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRC3 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..3654
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3607..3630
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aaaccggcac cttcggtgta ttgatggatg caagcccaag ggaacgcttg
     1261 gagggggacc tggaactgct ggccgtattt acacatgtag ttgtggccgt atccgtagcc
     1321 gccctggtgc ttaccgcggc gattctgctt tccttgcgca gtctgaaaag caatgttcgg
     1381 ggcatacacg ccaacgttgc tgcggctctt ggggtagccg aactcctgtt tctccttggg
     1441 attcatcgaa cgcacaatca gcttgtttgc acggcggttg ccatcctcct tcattatttc
     1501 tttcttagca ccttcgcttg gctgtttgtg caggggcttc atctgtaccg aatgcaagtt
     1561 gagcctagga acgtagatag gggtgcaatg aggttttatc atgccttggg gtggggtgtg
     1621 cccgctgtcc tccttggact tgcggtgggg ttggacccgg agggctacgg aaaccctgac
     1681 ttctgttgga ttagtgttca tgagccgctc atatggtctt ttgctggtcc ggtagtcttg
     1741 gtgattgtaa tgaacggtac tatgttcctt ctggctgctc gcacctcttg tagtactggc
     1801 caaagggaag caaaaaagac gagcgcgctg acattgcgat caagctttct tttgctgctt
     1861 cttgtaagcg cgtcctggtt gtttggtttg ctggccgtca accattcaat tctggccttt
     1921 cactatctgc acgcagggtt gtgtggactc caaggattgg cagtgcttct gctgttctgc
     1981 gttcttaacg ccgacgccag agcagcatgg atgccggctt gccttggacg caaggctgca
     2041 ccagaagagg ctagaccggc accaggcctg ggacctgggg catacaacaa caccgccttg
     2101 ttcgaagaaa gtgggcttat acggataact cttggcgcgt caactgtttc aagtgtaagc
     2161 tcagcacggt ctggccggac ccaagatcaa gactcacaac gcggccgcag ttaccttagg
     2221 gataacgttc tcgtgaggca tggtagcgca gctgaccaca ccgatcattc tctccaagcc
     2281 catgctggac ccactgacct cgacgttgcg atgttccacc gcgacgctgg tgccgacagt
     2341 gatagcgatt ccgacttgtc acttgaagag gagcggtctc tttctattcc atcttcagaa
     2401 agtgaagaca acggtagaac caggggtcgg ttccagagac cgctctgtcg cgccgcgcaa
     2461 tcagagagac tcttgaccca tcctaaggac gtagacggaa acgatctgtt gtcttattgg
     2521 cctgctcttg gagagtgtga ggcagcacca tgtgcacttc aaacatgggg aagtgagcgc
     2581 cggcttggcc tcgatacgag caaagacgcc gcgaacaata atcagcctga cccggctctt
     2641 actagcggtg atgaaacatc tctcggtagg gcacaacgac aacgcaaagg cattcttaag
     2701 aatcgcttgc agtatccact ggtgcctcag acccgcggag caccggagct ttcttggtgc
     2761 cgggcagcga ctctcggcca ccgggcggta cccgcggcgt cctacggccg gatttatgcg
     2821 ggaggaggca cgggaagcct ctcccagcct gcatcacgct attcaagccg agaacagttg
     2881 gacctcctgc tcagaagaca actgtcacgc gaacggcttg aagaagcccc agcgcctgtc
     2941 ttgagacctt tgagcaggcc cggctctcag gagtgcatgg acgcggcgcc cgggcgactg
     3001 gagcctaaag acaggggcag tacgcttcca cggcgacaac cacccagaga ctaccctggg
     3061 gccatggcag ggcggtttgg cagtagagac gcgcttgacc tgggcgctcc aagagagtgg
     3121 ctctccacac ttccacctcc gcgccgaaca cgagatctcg acccccaacc acccccgctt
     3181 cccctgtcac cacaacggca actctctcgc gatccgcttc tcccctcaag gccgcttgac
     3241 tcactcagta ggtccagcaa tagtagagaa caactcgacc aagtaccttc tcgccacccg
     3301 tcaagggagg ccctgggtcc gctgccccag ctcttgagag cgagagaaga ctccgtctca
     3361 ggcccgtccc atggtccctc tacagagcaa ctggatatac tgtcctccat ccttgcttcc
     3421 ttcaatagtt cagccctgtc ttctgttcag tccagcagca caccgcttgg cccccacacc
     3481 acagcaactc cttccgcgac ggcctccgtt ttgggaccct ccactcctcg atcagcaacg
     3541 tcacactcaa tctctgagct ctctcccgat tccgaagttc cccgatcaga agggcactct
     3601 ggttctgact acaaagacga tgacgacaag tgaagatctg acaaaactca caca
//
