LOCUS       sequence-424435-        2256 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2256)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 2256)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRD1 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..2256
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2209..2232
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aaactaattt cgctattctt atgcaggtgg tccctctgga gctcgccaga
     1261 gggcaccaag tggccctgtc cagcatctcc tatgtgggtt gcagtctcag tgtcctgtgc
     1321 ctggtcgcta ccttggttac attcgcggtc ctgtccagcg tgtccaccat ccggaaccag
     1381 cgctatcaca tccacgccaa cctgtctttc gccgtgctgg tcgcccaggt gctcctgctc
     1441 atttcctttc gtctggagcc tggaaccact ccatgccagg tgatggctgt gcttctgcac
     1501 tacttctttc tcagcgcctt cgcctggatg ctggtggagg gcttgcacct gtactctatg
     1561 gtgatcaagg tgtttggctc tgaagactca aagcatcgtt actattacgg tatgggttgg
     1621 ggattccccc ttttgatttg cattatcagt ctgagcttcg ctatggactc ctacggcact
     1681 tcaaataact gctggctgtc cctggcatct ggcgccatct gggcctttgt ggcccccgct
     1741 ctgttcgtga tcgtggtcaa tatcggcatc ctcattgcgg tcacaagggt catcagccag
     1801 atctctgcag ataactacaa gatccacggg gacccgtccg ccttcaagct gaccgcaaag
     1861 gctgtcgccg tcctcctgcc gattttgggc acgtcttggg ttttcggcgt tctcgctgtc
     1921 aacggatgtg ctgtcgtgtt ccagtatatg ttcgctaccc tgaatagcct gcaggggctc
     1981 ttcattttcc tgttccactg cctgctcaat agtgaggtga gggcggcttt caagcacaag
     2041 accaaggtgt ggagcctgac atcatcttcc gcccggactt ctaacgcgaa accgtttcat
     2101 agcgacctga tgaacgggac ccgcccgggg atggcttcca ccaagctctc tccctgggac
     2161 aagagctctc atagcgccca cagagtggac ctgtccgccg taggttctga ctacaaagac
     2221 gatgacgaca agtgaagatc tgacaaaact cacaca
//
