LOCUS       sequence-424444-        2247 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRF3 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..2247
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2200..2223
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aaaccgcttt ctctgttttg atgagccccc acaccgtgcc ggaggaaccc
     1261 gcgcttgcgc tgctcactca ggttggactg ggcgccagca tcttggcact tctggtctgc
     1321 ctgggagttt actggctggt gtggcgggtt gtcgtgcgca acaaaatttc ttatttccgc
     1381 cacgccgcgc tgttgaacat ggtgttttgc ctcctggctg ccgacacctg tttcctgggc
     1441 gcccccttcc tgagccccgg gccccgctct cctctgtgcc tggccgctgc ctttctgtgc
     1501 cactttctgt acctggcaac atttttctgg atgcttgccc aggctctggt gctggctcac
     1561 cagcttctgt tcgtgtttca ccagctcgca aaacacagag tgctgcccct gatggtgctc
     1621 ctgggatacc tgtgccccct tggcttggct ggcgttacac tgggcctgta cctccctcag
     1681 ggccagtatc tgcgcgaggg cgagtgctgg ctggacggta agggcggagc actgtacacc
     1741 tttgtgggcc ccgttctggc gattatcgga gtcaacgggc tggtcttggc catggcaatg
     1801 ctcaaattgc tgaggccttc tctttccgag ggtccgccag cagaaaagcg ccaggccctc
     1861 ctgggcgtca tcaaggccct ccttatcctc accccaatct tcgggttgac ctggggcctg
     1921 ggtcttgcga ccctgctcga agaggttagt accgtgcccc actatatctt caccatcctg
     1981 aatacactgc agggcgtctt tatcctgctt ttcggctgcc tgatggaccg gaaaatccag
     2041 gaagcgttgc gtaagcgctt ttgccgcgcc caggcaccga gctctactat cagtctcgtg
     2101 agttgttgcc tgcagatcct gagctgtgca agcaaatcta tgtctgaggg tatcccatgg
     2161 ccatcctctg aagatatggg taccgcccgc agtggttctg actacaaaga cgatgacgac
     2221 aagtgaagat ctgacaaaac tcacaca
//
