LOCUS       sequence-424445-        2199 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2199)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 2199)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRF4 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..2199
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1195..1215
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQM"
     CDS             2152..2175
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aaatgtcctt ttctattctg atgtcttcca agtccatgac cgataaggtg
     1261 ctggattaca tcacctgcat tggtctgtcc gtgtccatcc tgagcctggt gctctgtttg
     1321 attatcgaag ccaccgtgtg gagccgggtc gtagtgactg agatttccta catgcggcat
     1381 gtgtgcatcg tgaacattgc cgtgtctttg ctgaccgcaa atgtgtggtt cattatcggt
     1441 tctcatttta acatcaaggc ccaggattac aatatgtgcg tggcagtgac ctttttctcc
     1501 cattttttct atctgtctct gtttttctgg atgctgttca aggcgcttct gattatctac
     1561 ggaatcctgg tcattttcag gcgcatgatg aagagtcgca tgatggtcat cggctttgct
     1621 atcggatacg gctgtccact gattatcgcc gtgacaaccg ttgccatcac cgaacccgaa
     1681 aagggataca tgcgtcctga ggcctgctgg ctgaactggg acaacaccaa agccttgctg
     1741 gctttcgcca tccctgcctt tgttatcgtt gcggtgaact tgattgtggt cctggtagtg
     1801 gccgtgaaca ctcagcgccc ctccattgga agctccaaga gccaggacgt tgtgatcatt
     1861 atgaggatca gcaagaatgt cgcgatcctg accccgctgc ttgggcttac ctggggtttc
     1921 ggcatcgcta ccctgattga gggcaccagc ttgaccttcc acattatctt tgccctcctg
     1981 aatgctttcc aaggattttt cattctgctt ttcggaacga tcatggacca caagatccgc
     2041 gacgctctgc gcatgagaat gtctagcctg aaggggaaat ctagagcagc tgaaaacgca
     2101 tctctgggcc ccactaacgg ctcaaagctg atgaatcgcc agggaggttc tgactacaaa
     2161 gacgatgacg acaagtgaag atctgacaaa actcacaca
//
