LOCUS       sequence-424450-        2343 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2343)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 2343)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRG4 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..2343
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2296..2319
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aaacccactt tggcgtgctc atggacctct caagatctac cgtggactca
     1261 gtgaatgagc agatcctggc cctgatcacc tacaccgggt gtggcatcag tagcattttc
     1321 cttggcgtcg ctgtagtgac atacattgct ttccacaagc tgcgcaagga ttaccctgcg
     1381 aaaatcctga tcaacctctg tacagctctg ctcatgctga acctggtctt cttgatcaac
     1441 tcctggctct ccagcttcca gaaggtggga gtctgtatca cagctgcagt tgccctgcac
     1501 tatttcttgc ttgtctcatt tacatggatg ggacttgagg ccgtgcacat gtacctggcc
     1561 ctcgtcaagg ttttcaacat ctacatcccg aactacatcc tcaagttctg tctggtgggc
     1621 tggggaattc ccgcaatcat ggtggcaatt acggtctccg ttaaaaagga tctgtacggc
     1681 accctctccc cgacaacccc gttttgttgg atcaaggatg actccatctt ttatatcagc
     1741 gttgtggcct atttttgcct gatttttctg atgaacctgt ctatgttttg cacggtcctg
     1801 gtgcagctga attctgtaaa gtcccagatc caaaaaactc gccgtaaaat gatcctgcat
     1861 gacctgaaag gcaccatgtc cctcactttt ctgctcggcc tgacatgggg atttgccttc
     1921 tttgcctggg gaccgatgag aaatttcttt ttgtatcttt ttgcaatctt taacacactc
     1981 cagggatttt tcatctttgt attccattgt gttatgaagg agtcagtcag agagcaatgg
     2041 cagatccact tgtgctgtgg ctggctgcgt ctcgataact cctcagacgg aagctcccgc
     2101 tgtcagatta aggttggcta taagcaggaa ggtctgaaaa agatttttga gcacaaactg
     2161 ctcaccccgt ctctgaaatc taccgcaacc tcatccacct tcaaatccct gggcagcgcc
     2221 caaggcactc ccagcgaaat cagtttcccc aatgacgatt tcgacaaaga tccctattgc
     2281 agtagtccgg gttctgacta caaagacgat gacgacaagt gaagatctga caaaactcac
     2341 aca
//
