LOCUS       sequence-424452-        2409 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2409)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 2409)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRG6 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..2409
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2362..2385
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aaacacactt tggagttctg atggaccttc caagaagtgc ctcacagtta
     1261 gatgcaagaa acactaaagt cctcactttc atcagctata ttgggtgtgg aatatctgct
     1321 attttttcag cagcaactct cctgacatat gttgcttttg agaaattgcg aagggattat
     1381 ccctccaaaa tcttgatgaa cctgagcaca gccctgctgt tcctgaatct cctcttcctc
     1441 ctagatggct ggatcacctc cttcaatgtg gatggacttt gcattgctgt tgcagtcctg
     1501 ttgcatttct tccttctggc aacctttacc tggatggggc tagaagcaat tcacatgtac
     1561 attgctctag ttaaagtatt taacacttac attcgccgat acattctaaa attctgcatc
     1621 attggctggg gtttgcctgc cttagtggtg tcagttgttc tagcgagcag aaacaacaat
     1681 gaagtctatg gaaaagaaag ttatgggaaa gaaaaaggtg atgaattctg ttggattcaa
     1741 gatccagtca tattttatgt gacctgtgct gggtattttg gagtcatgtt ttttctgaac
     1801 attgccatgt tcattgtggt aatggtgcag atctgtggga ggaatggcaa gagaagcaac
     1861 cggaccctga gagaagaagt gttaaggaac ctgcgcagtg tggttagctt gacctttctg
     1921 ttgggcatga catggggttt tgcattcttt gcctggggac ccttaaatat ccccttcatg
     1981 tacctcttct ccatcttcaa ttcattacaa ggcttattta tattcatctt ccactgtgct
     2041 atgaaggaga atgttcagaa acagtggcgg cagcatctct gctgtggtag atttcggtta
     2101 gcagataact cagattggag taagacagct accaatatca tcaagaaaag ttctgataat
     2161 ctaggaaaat ctttgtcttc aagctccatt ggttccaact caacctatct tacatccaaa
     2221 tctaaatcca gctctaccac ctatttcaaa aggaatagcc acacagataa tgtctcctat
     2281 gagcattcct tcaacaaaag tggatcactc agacagtgct tccatggaca agtccttgtc
     2341 aaaactggcc catgcggttc tgactacaaa gacgatgacg acaagtgaag atctgacaaa
     2401 actcacaca
//
