LOCUS       sequence-424453-        2412 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2412)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 2412)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRG7 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..2412
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2365..2388
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aaaccaactt tgcagtgctt atgacattca agaaagacta ccaataccct
     1261 aaaagtcttg acatcctgtc taacgtcggt tgtgccctga gcgtcacagg gctcgcgttg
     1321 acggttattt ttcagatcgt tacccgcaag gtgagaaaga cctccgtgac atgggttctc
     1381 gtcaacctct gcatctctat gctgatcttc aacttgctgt tcgtgtttgg gatcgagaat
     1441 tccaataaga acctgcagac ctccgatgga gatatcaata acattgattt cgacaacaat
     1501 gatattccca ggaccgatac aattaatatc cccaacccca tgtgtacggc gatcgccgct
     1561 ctgctccact attttcttct ggtaaccttc acttggaacg ctctttccgc agctcagttg
     1621 tattaccttc tgatccgcac aatgaagcct ctgccgcgcc atttcatcct gttcattagt
     1681 ctgatcggat ggggagtacc cgctatcgta gtggcaatta ctgtcggagt aatctactct
     1741 caaaacggaa acaatcctca atgggagctg gactaccgtc aggaaaaaat ctgctggctg
     1801 gcaatcccgg aacctaacgg ggtgatcaag agtcctctcc tgtggtcatt catcgtgcct
     1861 gtgaccatca ttctgattag caacgtggtc atgttcatca caatcagcat caaagtgctc
     1921 tggaagaata accagaactt gactagcact aaaaaggtgt cctctatgaa aaagattgtg
     1981 tccacactgt ctgtggcggt tgtcttcggc atcacatgga tcctcgccta tctgatgctg
     2041 gtgaatgacg attctatccg catcgtgttc tcctacattt tctgcctttt caacacaacc
     2101 cagggcctgc agatctttat cctttacact gtgcgcacaa aggtcttcca gagcgaagca
     2161 agcaaggttt tgatgctcct gagttccatt ggtaggcgca aaagcctgcc cagcgtgact
     2221 agaccccgcc tgcgcgtcaa gatgtacaac tttctccgca gcttgcctac actgcatgag
     2281 cgctttcgcc tcttggagac cagtccctcc actgaagaga ttaccctgag cgaaagtgac
     2341 aacgctaagg agtctatcgg ttctgactac aaagacgatg acgacaagtg aagatctgac
     2401 aaaactcaca ca
//
