LOCUS       sequence-424455-        3171 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3171)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 3171)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRL2 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..3171
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3124..3147
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aaaccaactt cgctattctg atggcccacc gggagattgc ttacaaggat
     1261 ggtgtgcacg agcttttgct caccgtcatc acctgggtgg gtattgtgat cagtttggtt
     1321 tgcctcgcca tctgcatttt tactttttgt tttttcaggg gtcttcagtc tgaccggaat
     1381 actatccaca agaacttgtg catcaacctg ttcattgccg agttcatctt cttgatcggc
     1441 atcgacaaaa cgaaatacgc catcgcctgt ccgatctttg ctggtctgct ccactttttc
     1501 tttctggcag cctttgcttg gatgtgcctg gaaggcgttc agctgtacct gatgctggtg
     1561 gaggtgtttg aatccgagta ttctcgcaaa aagtattact atgttgctgg ttacctgttc
     1621 cctgccaccg tcgtgggcgt atctgcagct atcgactata aaagttacgg tacggagaag
     1681 gcctgctggc ttcacgtcga caactatttc atctggtcat tcatcggccc cgtgacgttc
     1741 attatccttc tgaacattat cttccttgtc atcactctct gtaaaatggt caagcacagc
     1801 aacacactga agcctgactc atctcgcctg gagaacatca agagttgggt tctgggcgca
     1861 ttcgccctgt tgtgtcttct ggggctgacc tggtccttcg gcttgctctt cattaacgaa
     1921 gagaccatcg ttatggccta ccttttcacc atcttcaacg ctttccaggg cgtctttatc
     1981 tttatcttcc actgtgcatt gcagaaaaag gttcgtaaag agtacggtaa gtgttttagg
     2041 cattcctatt gctgtggagg cctgcccaca gagtctccac acagctcagt aaaagcttct
     2101 accactagaa cctccgcccg ctactctagc ggtacccagt caagaatcag gcgcatgtgg
     2161 aacgataccg tgagaaaaca gtccgagtct agcttcatct ccggagacat taactccacg
     2221 tcaaccctga accagggcat gaccggcaac tatctgctta caaaccctct tctgcgtccc
     2281 cacgggacta ataacccata caatacgctt ctggccgaga ccgtggtatg caacgcaccc
     2341 agtgcccctg tcttcaattc tccgggtcac agcctgaata acgcaagaga cacgagtgct
     2401 atggacaccc tgccactgaa cggcaacttt aataactcat actcactgca caaaggtgat
     2461 tacaacgact ccgtgcaggt cgttgattgc ggcctgtctc tgaacgacac agccttcgag
     2521 aagatgatta tctctgagct ggtccacaat aacctgagag gcagctccaa gacccataac
     2581 ctggagctga ctctgcccgt gaaacccgtt attggtggct ccagctcaga ggatgacgct
     2641 atcgtggccg acgcctcatc cctgatgcat tccgacaacc ctgggctgga gctccatcac
     2701 aaggagctcg aagctcccct gatcccgcag agaacccatt ctctcctgta ccagccccag
     2761 aaaaaggtaa aaagtgaggg tacggatagc tatgtgtcac agctgaccgc cgaggccgag
     2821 gaccatctgc agagccccaa tcgcgacagc ctctacacat ccatgcccaa cctgcgtgac
     2881 tcaccctatc ccgaatcttc ccccgatatg gaagaggatc tgagcccgtc ccgtcgctct
     2941 gagaacgaag atatctacta taagagcatg ccgaatctgg gagccggtca tcagcttcaa
     3001 atgtgctatc agatctctcg gggaaattct gacggctaca ttatccctat caacaaagaa
     3061 ggatgtatcc cagaaggcga cgtgcgcgaa ggacaaatgc agctggtcac ctctctgggt
     3121 tctgactaca aagacgatga cgacaagtga agatctgaca aaactcacac a
//
