LOCUS       sequence-424456-        3084 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3084)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 3084)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRL3 CTF-FLAG
FEATURES             Location/Qualifiers
     source          1..3084
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3037..3060
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aaaccaattt cgctgtcttg atggcccatg tcgaagttaa acactccgat
     1261 gcggtccatg acttgctgtt ggatgtcatc acatgggtgg gtattctgtt gagcctggtg
     1321 tgtctgctca tctgtatctt cacattttgc ttctttaggg gtctgcagag cgacaggaac
     1381 acgatccata aaaacctgtg tatctcactg ttcgtggcgg aactcctgtt cctgattggc
     1441 attaaccgca ctgatcagcc tattgcctgc gcggtgttcg ctgccctgct tcactttttc
     1501 tttctggcag ccttcacttg gatgtttctc gagggcgttc agctgtatat catgctggtc
     1561 gaagtcttcg aatctgagca ttcacgccgg aagtatttct accttgtggg ctatggaatg
     1621 ccagccctca tcgttgccgt ttccgccgct gtggactacc gtagctacgg gacagataag
     1681 gtgtgctggc tgaggctgga cacatacttc atctggagtt tcatcggccc cgctaccctg
     1741 attatcatgc tgaacgtcat cttcctgggc atcgccctgt acaaaatgtt tcaccataca
     1801 gctatcctca agccggaatc tggctgtctc gacaacatta agtcctgggt tatcggtgct
     1861 atcgctttgc tgtgcctttt gggtctgact tgggcattcg gcctgatgta cattaacgaa
     1921 tccacagtca tcatggccta cctgtttacc atcttcaaca gcctgcaggg catgttcatc
     1981 tttattttcc actgtgtgct ccagaaaaag gtgcggaaag aatatgggaa atgtctgcgt
     2041 actcactgct gtagcggcaa gtccacggag tcttccattg gatccggcaa gaccagtggt
     2101 agccgcacac ccggaagata ctccactggt tcccagtcta gaatccgtcg catgtggaat
     2161 gacaccgtga gaaaacagag cgagagtagc ttcatcacgg gcgacatcaa ctccagtgcc
     2221 agtctgaaca gggagggact cctgaataac gctcgcgaca cctccgtcat ggacaccttg
     2281 cccctgaacg gcaaccacgg caactcatac agcatcgctt ccggcgagta tctctccaac
     2341 tgcgttcaga tcattgatcg cggctacaac cataacgaga ctgctctcga gaaaaagatc
     2401 ctgaaggaac tgaccagcaa ttacattccc agctatctga ataaccacga acgtagttcc
     2461 gagcaaaaca gaaatcttat gaacaaactc gtgaataacc tggggtccgg gagagaagat
     2521 gacgctatcg tgcttgacga tgctacctcc tttaaccacg aagagtcctt gggcctcgag
     2581 ctgatccacg aagagagcga tgccccattg ctgccacctc gggtctacag cacagagaac
     2641 caccagccgc accattacac ccgtaggaga atcccacagg atcactccga gtcctttttc
     2701 cctctgctca ctaacgaaca caccgaggat ctgcagagcc cccatcggga cagtttgtat
     2761 actagcatgc ccacactcgc tggcgtggcg gcaacagaat ccgtaaccac tagcacccag
     2821 acggaacctc cgcccgcgaa gtgcggcgat gccgaggatg tgtattacaa gagcatgcca
     2881 aacttgggtt cccgcaacca cgtgcaccag ctgcatacct attaccagct gggtaggggt
     2941 agtagcgacg gttttatcgt cccgcccaac aaggatggca cgccccctga aggctccagc
     3001 aagggccctg ctcatctggt tacctccctg ggttctgact acaaagacga tgacgacaag
     3061 tgaagatctg acaaaactca caca
//
