LOCUS       sequence-424461-        2997 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2997)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 2997)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRA3 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..2997
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2950..2973
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aagatctgac cgggtccgag ctgtacactc aagcggcttc tctgcttcac
     1261 cccgttgtgt acaccactgc tattatcctg ttgctgtgct tgctggctgt tatcgttagc
     1321 tacatttatc atcacagcct catccgtatc tctcttaagt catggcatat gctggttaat
     1381 ctgtgtttcc acatctttct gacctgcgtt gtgttcgttg ggggcattac acagaccagg
     1441 aacgcttcta tctgccaggc ggttggcatt atcctccatt actccaccct ggctactgtg
     1501 ctctgggtag gggtcaccgc ccgcaatatc tacaagcagg tcacaaagaa agctaagcgc
     1561 tgccaggatc cggatgaacc ccctccccct ccccgtccaa tgttgcgttt ttacctgatc
     1621 gggggcggta tccctattat cgtgtgcggc atcaccgcag ccgctaacat caagaattac
     1681 ggctcccgcc cgaacgcccc atactgctgg atggcgtggg aaccctccct tggcgctttc
     1741 tatgggccag cttctttcat cacattcgtc aactgtatgt actttctgtc cattttcatt
     1801 cagttgaaaa gacaccccga gcgtaagtac gagctcaagg agccgacaga agagcaacag
     1861 cgcctggcag ccaacgagaa cggagaaatt aaccaccagg acagcatgag tctctccctg
     1921 atcagcacat ctgccctcga gaacgaacac accttccact ctcagctgtt gggcgccagc
     1981 ttgaccctgc tcctgtacgt ggcgctgtgg atgttcggcg ctctggccgt ctccctgtac
     2041 tatccactgg acctggtgtt ctcatttgtg tttggtgcca cctccctgag tttcagcgct
     2101 tttttcgtcg tgcatcactg tgtgaaccgc gaagacgttc gtttggcttg gatcatgacc
     2161 tgttgcccgg gcaggtctag ctattccgtg caggtgaatg tccagccccc taactctaac
     2221 ggtaccaacg gggaggcgcc caagtgtccg aactcttccg ctgagtctag ttgcactaac
     2281 aaatctgctt ctagcttcaa aaactcatct caggggtgca agttgacaaa tcttcaggcc
     2341 gcggctgccc agtgccatgc aaactctctg cctttgaaca gcaccccaca gctggacaac
     2401 tccctgactg agcactccat ggacaacgac attaaaatgc acgtggctcc attggaggtg
     2461 caattccgca ctaatgtaca ctcttccagg catcacaaga accggagcaa aggccataga
     2521 gcgtctcgcc tgactgtgct ccgcgagtat gcctacgatg tgccgactag tgtcgagggt
     2581 tccgtacaaa atggcctgcc caagagtcgc ctcggtaata acgaggggca cagcaggtct
     2641 cgtcgggcct acctcgcata ccgtgagcgt cagtacaatc cccctcaaca ggactcctct
     2701 gatgcctgta gtaccctgcc aaagtctagc agaaacttcg aaaagccagt cagcacaacc
     2761 agcaaaaagg acgcccttcg gaagcctgct gtggttgaac tcgagaacca acagaaaagt
     2821 tacggtctta acctcgcgat ccaaaacggt ccaatcaagt ccaacggaca ggaggggccc
     2881 ttgctgggca cagacagcac aggcaacgtt cggaccggtt tgtggaagca cgaaaccacc
     2941 gtcggttctg actacaaaga cgatgacgac aagtgaagat ctgacaaaac tcacaca
//
