LOCUS       sequence-424463-        3267 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3267)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 3267)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRB2 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..3267
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3220..3243
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aacagccgcc caaggacctg acgctggagc tggccggcag ccccagcgtc
     1261 ccgctcgtca tcggctgtgc agtgtcttgt atggcgttgc tcaccctcct ggccatttat
     1321 gcggctttct ggcgctttat caagtccgag cgttctatca ttctcctgaa cttctgtctt
     1381 agtatcctgg cctccaacat cctcattctc gtgggccaga gtcgcgtcct gagtaaaggc
     1441 gtatgtacta tgactgctgc gttcctccac ttctttttcc tcagcagttt ctgctgggtg
     1501 ctcaccgaag catggcagtc ttacctggcc gtgatcggcc gtatgagaac acgcctggtg
     1561 cgcaagcgct ttctgtgtct gggctggggc ctgccagctc tggtggtagc tgtgagcgtg
     1621 gggtttacac gcacaaaggg ctacggcacc tccagctact gttggctgag cctggaggga
     1681 gggctgctct atgcattcgt aggcccggcc gctgtgatcg tcctggtcaa catgctcatc
     1741 ggtattatcg tcttcaataa actgatggcc cgggatggga tctctgacaa gtccaagaaa
     1801 cagagggcag ggagtgagag gtgcccctgg gcgtctctgc ttctgccttg tagtgcctgc
     1861 ggggctgttc caagccccct gcttagctct gcttctgcta gaaacgcgat ggccagcctg
     1921 tggagctctt gcgtggtcct gccactcctg gctctgactt ggatgagtgc cgttcttgcg
     1981 atgactgaca ggcgctcagt gctgttccag gctctgttcg cagtattcaa ttccgcccag
     2041 ggctttgtga tcaccgcagt tcactgcttc ctccgccgtg aggtgcagga cgtcgtgaaa
     2101 tgtcagatgg gcgtgtgcag agccgatgaa tccgaggatt cccccgacag ctgcaagaac
     2161 ggacagctgc agatcctgtc cgacttcgag aaagatgttg accttgcctg ccagacagtt
     2221 ctgttcaagg aggtgaatac ttgtaacccg agtaccatca ctgggactct gtccaggctg
     2281 tccctggatg aagacgaaga gccaaagtct tgtcttgtgg gccccgaggg aagcctgtcc
     2341 ttctcaccct tgcccggcaa catccttgta cccatggccg ctagccctgg ccttggtgag
     2401 ccccctcccc ctcaggaagc caacccggtg tatatgtgcg gcgagggtgg cctccgccaa
     2461 ttggacctga cctggctgcg tcctacggaa ccaggcagcg agggcgatta catggttctg
     2521 cctagacgga ctctgagcct gcagcccgga ggcggtggag gcggtggaga ggacgcacca
     2581 cgggctaggc cggaaggcac accgagacgc gcggccaaga ctgtcgctca tacagagggg
     2641 tatccatcct ttctttccgt ggaccattcc gggctgggct tgggaccggc ttacggttcc
     2701 ttgcagaacc cttacggcat gacgtttcag ccacctcccc ctaccccatc cgccagacag
     2761 gttcctgagc ctggcgagag gtccagaaca atgccacgga ccgtacccgg gtccacaatg
     2821 aagatgggaa gtcttgaaag aaagaaactg cgctacagtg acctggattt cgagaaggtg
     2881 atgcataccc gcaaaagaca ctccgagctg tatcatgagc tgaaccagaa atttcacaca
     2941 ttcgaccggt accggtccca gtccaccgcc aagcgggaga agcgctggtc tgtcagctct
     3001 ggaggtgctg ccgagcgtag cgtatgtacc gataaaccaa gccctggaga gcgcccctcc
     3061 ctgtcccagc acaggcgcca tcagagctgg agcacattta aatccatgac tttgggtagc
     3121 ttgccaccta agccacgcga acgtctgacg ctgcaccgtg ctgccgcgtg ggagcctacc
     3181 gaaccgccag acggtgattt tcaaaccgag gttggttctg actacaaaga cgatgacgac
     3241 aagtgaagat ctgacaaaac tcacaca
//
