LOCUS       sequence-424464-        3243 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3243)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 3243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRB3 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..3243
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             3196..3219
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aacagcaacc tagagaaata atcatggaat cctctggcac accttcagtt
     1261 accctaatag taggcagtgg tctttcttgc ttggccttga ttaccctagc agttgtctat
     1321 gcagcattat ggaggtacat acgctctgag agatccataa tactaattaa cttctgcctg
     1381 tctatcatct catccaatat cctcatactg gttggacaga ctcagacaca taataagagt
     1441 atctgcacaa ccaccactgc atttttgcac tttttcttcc tggcttcatt ctgttgggtt
     1501 ttgactgagg cgtggcaatc atatatggct gtaactggaa aaattaggac acggcttata
     1561 agaaaacgct ttttgtgcct tggatggggt ttaccagcat tagtagtggc cacatcagta
     1621 ggcttcacca gaacaaaagg atatggcact gatcactact gctggctctc tcttgaagga
     1681 ggactactct atgcttttgt gggacctgca gccgctgttg tcctggtcaa catggtgatt
     1741 ggcattttgg tatttaataa acttgtttcc agagatggaa tcctagataa aaagctcaaa
     1801 cacagagccg gtcagatgag tgagcctcat agcggtttga cgctcaaatg tgccaagtgt
     1861 ggagtagttt caacaacagc tttgtcagcc accaccgcca gtaacgccat ggcgtctctt
     1921 tggagctcct gtgtggtgtt gccccttctg gctttgacgt ggatgtctgc ggttctggcc
     1981 atgacagata aacgctccat attgtttcaa atactttttg ctgtgtttga ttcattgcaa
     2041 ggctttgtta tagtcatggt ccactgcatt cttcggagag aggttcagga tgcatttaga
     2101 tgccgattga gaaactgtca ggatcccatc aatgcagatt cttcgagttc gtttcctaat
     2161 gggcatgctc aaatcatgac agactttgaa aaggatgtag acattgcctg tcgatcagtt
     2221 cttcataagg atattggtcc ttgccgagca gccacaataa caggaacact ttctaggatt
     2281 tctctaaatg atgatgaaga agaaaaggga acaaaccctg aagggctaag ctattcaaca
     2341 ttgcctggaa atgtcatttc caaagtcatc atccagcaac ccacaggttt gcacatgccc
     2401 atgagtatga atgagcttag caatccatgt ttgaaaaaag aaaatagtga attgcggaga
     2461 actgtgtact tatgtacgga tgataatttg agaggggctg acatggacat agtccatcct
     2521 caagaaagaa tgatggaaag tgactatatt gtgatgccca gaagttctgt aaataaccag
     2581 ccttcaatga aagaagaaag caaaatgaat attggcatgg aaaccttgcc gcatgaaagg
     2641 ctattgcact acaaagtaaa ccctgaattc aatatgaatc cccctgtaat ggaccagttc
     2701 aatatgaact tagagcaaca tctcgcaccc caggaacata tgcagaattt gccctttgaa
     2761 cctcgcacag ctgtgaagaa tttcatggcc tctgagttgg atgataatgc aggactatca
     2821 agaagtgaaa ctggatcaac gatatcaatg agttctttag agagaagaaa atcacgatat
     2881 tcagaccttg actttgagaa ggtcatgcat acaaggaaga ggcatatgga actatttcaa
     2941 gaactaaatc agaaatttca aactttggac agatttcggg atataccaaa tacaagcagt
     3001 atggaaaacc ccgcaccaaa caagaatcca tgggacactt tcaaaaaccc cagtgaatac
     3061 ccgcattaca ccacaatcaa tgtcttagac acagaggcaa aggatgcttt ggaactgagg
     3121 ccagcagagt gggagaagtg tctgaatttg cctctggatg tgcaagaggg tgactttcaa
     3181 acagaagttg gttctgacta caaagacgat gacgacaagt gaagatctga caaaactcac
     3241 aca
//
