LOCUS       sequence-424465-        2943 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2943)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 2943)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRC1 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..2943
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2896..2919
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aagacatcag ccgtagagag aacggagaag tgctgcctct gaaaatcgtg
     1261 acatacgccg ccgtgtccct ctccctggct gccctgctgg tggctttcgt gctgctgtct
     1321 ctggtgcgga tgcttcggag caacctgcat agcatccaca agcacctggc cgtggccctg
     1381 ttcctgagcc agctggtctt tgtgattggc atcaaccaga cagagaatcc tttcctgtgt
     1441 acagtggtgg ccatcctgct ccactatatc tacatgagca cattcgcctg gactctggtg
     1501 gaaagcctgc acgtgtacag aatgctgaca gaggtgcgca acattgacac cggccctatg
     1561 cggttctact acgtggtcgg atggggcatc cccgctatcg tgaccggcct ggccgtgggc
     1621 ttggatcccc agggctacgg caaccccgac ttctgctggc tgagcttgca ggacaccctg
     1681 atctggtctt tcgccggccc catcggcgcc gtaatcatca tcaacaccgt tacatctgtg
     1741 ctgtctgcta aggtgtcctg tcagagaaag caccactact acggaaaaaa gggcatcgtg
     1801 agcctgctgc gaaccgcctt cctgctactg ctcctgatca gcgccacatg gctgctgggc
     1861 ctgctggccg tgaacagaga tgccctgagc tttcactacc tgtttgccat cttcagcggc
     1921 ctgcagggcc ccttcgtgct gctgttccac tgcgtgctga accaggaggt gagaaagcac
     1981 ctgaagggcg tgctgggagg caggaagctg cacctggaag attccgccac cacaagagcc
     2041 accctgctga ccagaagcct gaactgcaac accaccttcg gcgacggacc tgacatgctg
     2101 agaacagatc tgggcgagtc taccgccagc ctggactcca tcgtgcggga cgagggcatc
     2161 cagaaactgg gagtttcctc cggcctggtg cggggatccc acggcgaacc cgacgccagc
     2221 ctcatgcctc ggagctgcaa ggacccacca ggccacgaca gcgattccga cagcgagctg
     2281 agcctggacg agcagagcag cagctacgcc tctagccaca gctctgatag cgaggacgac
     2341 ggcgtgggcg ccgaggaaaa gtgggacccc gccagaggcg ccgtgcacag cacccctaag
     2401 ggcgatgccg ttgctaatca cgtgccagct ggctggcctg atcaatctct ggctgagagc
     2461 gacagcgagg accccagcgg caagcctaga ctgaaggtgg aaaccaaggt gtctgtggaa
     2521 ctgcacagag aggaacaagg gagccataga ggcgagtacc ctcctgatca ggagagcggc
     2581 ggcgcggcta gactggcctc cagccagcct cctgagcagc ggaaaggcat cctgaagaac
     2641 aaggtcacat atcctcctcc actgaccctg accgagcaga ccctgaaggg aagactgaga
     2701 gaaaagctgg ctgattgcga gcaaagtcct accagctcta gaaccagctc cctgggaagc
     2761 ggaggtcctg attgcgccat cacagtgaag agccccggca gagaacctgg ccgggaccac
     2821 ctaaatggcg tcgccatgaa cgtgcggacc ggctctgccc aggccgacgg cagcgactct
     2881 gaaaaacctg gttctgacta caaagacgat gacgacaagt gaagatctga caaaactcac
     2941 aca
//
