LOCUS       sequence-424488-        3150 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3150)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 3150)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRL2 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..3150
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1195..1215
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQA"
     CDS             3103..3126
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aagcccaccg ggagattgct tacaaggatg gtgtgcacga gcttttgctc
     1261 accgtcatca cctgggtggg tattgtgatc agtttggttt gcctcgccat ctgcattttt
     1321 actttttgtt ttttcagggg tcttcagtct gaccggaata ctatccacaa gaacttgtgc
     1381 atcaacctgt tcattgccga gttcatcttc ttgatcggca tcgacaaaac gaaatacgcc
     1441 atcgcctgtc cgatctttgc tggtctgctc cactttttct ttctggcagc ctttgcttgg
     1501 atgtgcctgg aaggcgttca gctgtacctg atgctggtgg aggtgtttga atccgagtat
     1561 tctcgcaaaa agtattacta tgttgctggt tacctgttcc ctgccaccgt cgtgggcgta
     1621 tctgcagcta tcgactataa aagttacggt acggagaagg cctgctggct tcacgtcgac
     1681 aactatttca tctggtcatt catcggcccc gtgacgttca ttatccttct gaacattatc
     1741 ttccttgtca tcactctctg taaaatggtc aagcacagca acacactgaa gcctgactca
     1801 tctcgcctgg agaacatcaa gagttgggtt ctgggcgcat tcgccctgtt gtgtcttctg
     1861 gggctgacct ggtccttcgg cttgctcttc attaacgaag agaccatcgt tatggcctac
     1921 cttttcacca tcttcaacgc tttccagggc gtctttatct ttatcttcca ctgtgcattg
     1981 cagaaaaagg ttcgtaaaga gtacggtaag tgttttaggc attcctattg ctgtggaggc
     2041 ctgcccacag agtctccaca cagctcagta aaagcttcta ccactagaac ctccgcccgc
     2101 tactctagcg gtacccagtc aagaatcagg cgcatgtgga acgataccgt gagaaaacag
     2161 tccgagtcta gcttcatctc cggagacatt aactccacgt caaccctgaa ccagggcatg
     2221 accggcaact atctgcttac aaaccctctt ctgcgtcccc acgggactaa taacccatac
     2281 aatacgcttc tggccgagac cgtggtatgc aacgcaccca gtgcccctgt cttcaattct
     2341 ccgggtcaca gcctgaataa cgcaagagac acgagtgcta tggacaccct gccactgaac
     2401 ggcaacttta ataactcata ctcactgcac aaaggtgatt acaacgactc cgtgcaggtc
     2461 gttgattgcg gcctgtctct gaacgacaca gccttcgaga agatgattat ctctgagctg
     2521 gtccacaata acctgagagg cagctccaag acccataacc tggagctgac tctgcccgtg
     2581 aaacccgtta ttggtggctc cagctcagag gatgacgcta tcgtggccga cgcctcatcc
     2641 ctgatgcatt ccgacaaccc tgggctggag ctccatcaca aggagctcga agctcccctg
     2701 atcccgcaga gaacccattc tctcctgtac cagccccaga aaaaggtaaa aagtgagggt
     2761 acggatagct atgtgtcaca gctgaccgcc gaggccgagg accatctgca gagccccaat
     2821 cgcgacagcc tctacacatc catgcccaac ctgcgtgact caccctatcc cgaatcttcc
     2881 cccgatatgg aagaggatct gagcccgtcc cgtcgctctg agaacgaaga tatctactat
     2941 aagagcatgc cgaatctggg agccggtcat cagcttcaaa tgtgctatca gatctctcgg
     3001 ggaaattctg acggctacat tatccctatc aacaaagaag gatgtatccc agaaggcgac
     3061 gtgcgcgaag gacaaatgca gctggtcacc tctctgggtt ctgactacaa agacgatgac
     3121 gacaagtgaa gatctgacaa aactcacaca
//
