LOCUS       sequence-424489-        3063 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 3063)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 3063)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRL3 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..3063
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             1195..1215
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQA"
     CDS             3016..3039
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aagcccatgt cgaagttaaa cactccgatg cggtccatga cttgctgttg
     1261 gatgtcatca catgggtggg tattctgttg agcctggtgt gtctgctcat ctgtatcttc
     1321 acattttgct tctttagggg tctgcagagc gacaggaaca cgatccataa aaacctgtgt
     1381 atctcactgt tcgtggcgga actcctgttc ctgattggca ttaaccgcac tgatcagcct
     1441 attgcctgcg cggtgttcgc tgccctgctt cactttttct ttctggcagc cttcacttgg
     1501 atgtttctcg agggcgttca gctgtatatc atgctggtcg aagtcttcga atctgagcat
     1561 tcacgccgga agtatttcta ccttgtgggc tatggaatgc cagccctcat cgttgccgtt
     1621 tccgccgctg tggactaccg tagctacggg acagataagg tgtgctggct gaggctggac
     1681 acatacttca tctggagttt catcggcccc gctaccctga ttatcatgct gaacgtcatc
     1741 ttcctgggca tcgccctgta caaaatgttt caccatacag ctatcctcaa gccggaatct
     1801 ggctgtctcg acaacattaa gtcctgggtt atcggtgcta tcgctttgct gtgccttttg
     1861 ggtctgactt gggcattcgg cctgatgtac attaacgaat ccacagtcat catggcctac
     1921 ctgtttacca tcttcaacag cctgcagggc atgttcatct ttattttcca ctgtgtgctc
     1981 cagaaaaagg tgcggaaaga atatgggaaa tgtctgcgta ctcactgctg tagcggcaag
     2041 tccacggagt cttccattgg atccggcaag accagtggta gccgcacacc cggaagatac
     2101 tccactggtt cccagtctag aatccgtcgc atgtggaatg acaccgtgag aaaacagagc
     2161 gagagtagct tcatcacggg cgacatcaac tccagtgcca gtctgaacag ggagggactc
     2221 ctgaataacg ctcgcgacac ctccgtcatg gacaccttgc ccctgaacgg caaccacggc
     2281 aactcataca gcatcgcttc cggcgagtat ctctccaact gcgttcagat cattgatcgc
     2341 ggctacaacc ataacgagac tgctctcgag aaaaagatcc tgaaggaact gaccagcaat
     2401 tacattccca gctatctgaa taaccacgaa cgtagttccg agcaaaacag aaatcttatg
     2461 aacaaactcg tgaataacct ggggtccggg agagaagatg acgctatcgt gcttgacgat
     2521 gctacctcct ttaaccacga agagtccttg ggcctcgagc tgatccacga agagagcgat
     2581 gccccattgc tgccacctcg ggtctacagc acagagaacc accagccgca ccattacacc
     2641 cgtaggagaa tcccacagga tcactccgag tcctttttcc ctctgctcac taacgaacac
     2701 accgaggatc tgcagagccc ccatcgggac agtttgtata ctagcatgcc cacactcgct
     2761 ggcgtggcgg caacagaatc cgtaaccact agcacccaga cggaacctcc gcccgcgaag
     2821 tgcggcgatg ccgaggatgt gtattacaag agcatgccaa acttgggttc ccgcaaccac
     2881 gtgcaccagc tgcataccta ttaccagctg ggtaggggta gtagcgacgg ttttatcgtc
     2941 ccgcccaaca aggatggcac gccccctgaa ggctccagca agggccctgc tcatctggtt
     3001 acctccctgg gttctgacta caaagacgat gacgacaagt gaagatctga caaaactcac
     3061 aca
//
