LOCUS       sequence-424491-        2493 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of Adhesion G Protein-Coupled Receptor 
            transmembrane domains.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Dates AN, Jones DTD, Smith JS, Skiba MA, Rich MF, Burruss MM, Kruse 
            AC, Blacklow SC
  TITLE     Heterogeneity of Tethered Agonist Signaling in Adhesion G 
            Protein-Coupled Receptors
  JOURNAL   bioRxiv 2022.07.14.500097
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "bioRxiv 
            2022.07.14.500097"
COMMENT     Sequence Label: HA-MBP-TEVpCS-ADGRV1 CTFdeltaTA-FLAG
FEATURES             Location/Qualifiers
     source          1..2493
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     sig_peptide     1..60
                     /codon_start=1
                     /product="human interleukin-2 signal sequence"
                     /label=IL-2 signal sequence
                     /translation="MYRMQLLSCIALSLALVTNS"
     CDS             61..87
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             2446..2469
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 atgtacagga tgcaactcct gtcttgcatt gcactaagtc ttgcacttgt cacgaattcg
       61 tacccatacg atgttccaga ttacgctgga tccaaaattg aagaaggcaa actggtgatt
      121 tggattaacg gcgataaagg ctataacggc ctggcggaag tgggcaaaaa atttgaaaaa
      181 gataccggca ttaaagtgac cgtggaacat ccggataaac tggaagaaaa atttccgcag
      241 gtggcggcga ccggcgatgg cccggatatt attttttggg cgcatgatcg ctttggcggc
      301 tatgcgcaga gcggcctgct ggcggaaatt accccggata aagcgtttca ggataaactg
      361 tatccgttta cctgggatgc ggtgcgctat aacggcaaac tgattgcgta tccgattgcg
      421 gtggaagcgc tgagcctgat ttataacaaa gatctgctgc cgaacccgcc gaaaacctgg
      481 gaagaaattc cggcgctgga taaagaactg aaagcgaaag gcaaaagcgc gctgatgttt
      541 aacctgcagg aaccgtattt tacctggccg ctgattgcgg cggatggcgg ctatgcgttt
      601 aaatatgaaa acggcaaata tgatattaaa gatgtgggcg tggataacgc gggcgcgaaa
      661 gcgggcctga cctttctggt ggatctgatt aaaaacaaac atatgaacgc ggataccgat
      721 tatagcattg cggaagcggc gtttaacaaa ggcgaaaccg cgatgaccat taacggcccg
      781 tgggcgtgga gcaacattga taccagcaaa gtgaactatg gcgtgaccgt gctgccgacc
      841 tttaaaggcc agccgagcaa accgtttgtg ggcgtgctga gcgcgggcat taacgcggcg
      901 agcccgaaca aagaactggc gaaagaattt ctggaaaact atctgctgac cgatgaaggc
      961 ctggaagcgg tgaacaaaga taaaccgctg ggcgcggtgg cgctgaaaag ctatgaagaa
     1021 gaactggcga aagatccgcg cattgcggcg accatggaaa acgcgcagaa aggcgaaatt
     1081 atgccgaaca ttccgcagat gagcgcgttt tggtatgcgg tgcgcaccgc ggtgattaac
     1141 gcggcgagcg gccgccagac cgtggatgaa gcgctgaaag atgcgcagac caacgagaac
     1201 ctttacttcc aacgtactga caatctctct agctacaatg aagccttttt cacaagcgga
     1261 ttcatctgta tcagcggtct ctgcctggca gtgctgtccc acatcttctg tgcgagatac
     1321 agcatgtttg ccgctaagtt gctgacccac atgatggctg ccagcctggg cacccagatt
     1381 ctgttcctcg ccagcgctta tgcctcaccc cagctggctg aggaatcctg ttccgctatg
     1441 gctgccgtca cccactacct ctacctgtgc cagttctcct ggatgctgat ccagagtgtg
     1501 aacttctggt acgtcttggt catgaatgac gagcacaccg agcggaggta tttgctgttt
     1561 ttcctcctgt cttggggcct gccggcgttc gtcgtgatcc ttctgatcgt gatcctgaag
     1621 ggcatctacc accaatccat gtcccagatc tatggcctga ttcacggcga tctctgcttt
     1681 atccctaacg tttacgctgc cctcttcacc gctgcgctgg tgccactgac ttgtctcgtc
     1741 gtagtcttcg tcgtatttat ccatgcctat caggttaaac cccagtggaa ggcatacgac
     1801 gatgtcttcc gcggacgtac aaatgccgcg gaaatccccc tgatcttgta cttgttcgca
     1861 ttgattagcg tgacctggct ttggggtggc ctgcatatgg cgtatcgtca cttttggatg
     1921 ctggtgctgt tcgtcatttt taacagcttg cagggcttgt acgtgttcat ggtctatttt
     1981 atcctgcata accagatgtg ttgcccgatg aaggccagct ataccgtgga gatgaatggt
     2041 catcctggtc cctccacagc ctttttcact ccgggcagcg ggatgccacc ggccggaggc
     2101 gaaatctcca agtctacaca aaatctgatc ggtgccatgg aagaggtacc cccggattgg
     2161 gagcgggctt cctttcaaca gggcagccag gcttcccctg atctgaagcc ctcaccccag
     2221 aacggagcta ctttccctag ttctggaggc tatggccaag ggagcctgat cgcggatgaa
     2281 gagtctcagg agttcgatga cttgatcttc gcgctgaaga caggcgccgg actctccgtc
     2341 tctgacaatg aatcaggtca aggaagccag gagggtggca ccctgaccga ctctcagatt
     2401 gtggagctgc ggagaattcc aattgccgac acccatctgg gttctgacta caaagacgat
     2461 gacgacaagt gaagatctga caaaactcac aca
//
